Xiaoxiao Hou , Jidong Gu , Jicong Chen , Habib Ullah , Tursunay Mamtimin , Xing Wang , Xiaoqin Li , Yuchao Jiang , Jingwen Fan , Huawen Han , Xiangkai Li
{"title":"Identification and functional characterization of a novel CaSrpA enzyme for selenite reduction and selenium nanoparticle formation","authors":"Xiaoxiao Hou , Jidong Gu , Jicong Chen , Habib Ullah , Tursunay Mamtimin , Xing Wang , Xiaoqin Li , Yuchao Jiang , Jingwen Fan , Huawen Han , Xiangkai Li","doi":"10.1016/j.jhazmat.2025.138486","DOIUrl":"10.1016/j.jhazmat.2025.138486","url":null,"abstract":"<div><div>Selenite reductases are widely distributed across various oxidoreductase families (e.g., ThxR, OYE, and FccA enzymes) <span><span>[1]</span></span>. The ability of short-chain dehydrogenase/reductase (SDR) family enzymes for selenite reduction remains unknown. Using metagenomic and metatranscriptomic analyses, 40 putative selenite reductases were identified from selenium-rich regions based on catalytic domain homology and transcriptional upregulation. These enzymes mainly belong to the SDR family and metalloenzymes. Enzyme activity analysis indicated that <em>Ca</em>SrpA possessed the ability (<em>V</em><sub>max</sub>, 18.85 μM/min/g) to reduce selenite to selenium nanoparticles (SeNPs). Phylogenetic analysis showed that <em>Ca</em>SrpA was clustered in the clade of SDR enzymes, with the typical Rossmann fold. <em>Ca</em>SrpA also oxidized S-1-phenylethanol to phenylacetone (<em>V</em><sub>max</sub>, 15.4 μM/min/mg), sharing 53 % sequence similarity with the alcohol dehydrogenase RasADH. Molecular docking and structural superposition identified sixteen key residues associated with <em>Ca</em>SrpA activity. Site-directed mutagenesis revealed that over 14 mutants exhibited a 30–90 % reduction in relative activity. Mutant M206A enhanced catalytic efficiency towards selenite by 2.4-fold and S-1-phenylethanol by 5.4-fold via a lid-opening mechanism. Molecular dynamics simulation elucidated that the mutant M206A utilized lid opening mechanism to accommodate more substrate and co-factor for catalysis via altering the conformation of the α7-α8 loop. This study helps understand the intrinsic connection between the SDR family and selenite-reducing capability, broadening the repertoire of selenite reductases.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"494 ","pages":"Article 138486"},"PeriodicalIF":12.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Huanxin Zhang , Yu Zhang , Shixuan Cui , Lihua Cui , Jiaxin Li , Jizhuang Chu , Yumiao Zhou , Youru Zhang , Qiang Kong
{"title":"Pentachlorophenol causes energy deficiency and liver injury in marine medaka (Oryzias melastigma) through damaging mitochondria and inducing oxidative stress","authors":"Huanxin Zhang , Yu Zhang , Shixuan Cui , Lihua Cui , Jiaxin Li , Jizhuang Chu , Yumiao Zhou , Youru Zhang , Qiang Kong","doi":"10.1016/j.jhazmat.2025.138469","DOIUrl":"10.1016/j.jhazmat.2025.138469","url":null,"abstract":"<div><div>Pentachlorophenol (PCP) is widely distributed in marine environments and poses a threat to the health of marine organisms. Recent studies have demonstrated that PCP induces energy deficiency in marine organisms. However, the underlying toxification mechanism and the resulting adverse outcomes remain unclear. This study analyzed the energy metabolism of marine medaka (<em>Oryzias melastigma</em>) exposed to PCP at environmentally relevant concentrations via proteomic analysis and multiple physiological assessments. The results showed that PCP disrupted mitochondrial morphology, reduced mitochondrial membrane potential (MMP), and decreased adenosine triphosphate (ATP) levels in marine medaka. Proteomic analysis revealed that PCP induced oxidative stress, cytoskeletal disruption, and inhibition of the cytochrome bc1 complex and ATP synthase. These effects culminated in structural and functional impairments to mitochondria, which in turn inhibited ATP synthesis. Chronic PCP exposure led to enhanced glycolytic activity, the accumulation of liver lipids, and reduced liver function. The present study has deepened our understanding of the mechanism of PCP-induced energy production deficiency in marine fish and provides new insights into ecological risk assessment for PCP.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"494 ","pages":"Article 138469"},"PeriodicalIF":12.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Microplastics indirectly affect soil respiration of different-aged forest by altering microbial communities and carbon metabolism","authors":"Wen Hu, Zhenming Zhang, Guiting Mu","doi":"10.1016/j.jhazmat.2025.138532","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.138532","url":null,"abstract":"This study explored how microplastics impact soil respiration processes in forests of varying ages by modulating the structure of microbial communities and carbon metabolic functions. The findings indicated that the abundance of microplastics in different aged forests was approximately 600~3858 items∙kg<sup>-1</sup>. S10 exhibited the lowest, and S60 had the highest microplastic abundance. The microplastics mainly consisted of fibers (26.57% to 38.38%), particles sized 0-0.1 μm (40.28% to 70.19%), and black particles (6.92% to 43.46%). The soil respiration rate decreases with increasing forest age. However, the microplastics indirectly modified soil respiration by influencing total organic carbon (TOC) and soil pH. The functional prediction analysis showed that metabolic pathways such as formaldehyde assimilation, ribulose monophosphate pathway, and the hydroxypropionate-hydroxybutylate cycle, were significantly correlated with microplastic abundance. Structural equation model (SEM) results suggested that microplastics affected microbial carbon metabolic demands by altering microbial community structure (0.44) or directly influencing carbon metabolic pathways (0.68). Consequently, this impacts soil CO<sub>2</sub> emissions. The findings provide new insights into the critical role of forest soils in mitigating carbon emissions caused by microplastics.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"227 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhen Zhang, Xinxin Fan, Rumeng Zhang, Xinghui Pan, Xuexue Zhang, Yi Ding, Ying Liu
{"title":"Biodegradation Characterization and Mechanism of Low-Density Polyethylene by the Enriched Mixed-Culture from Plastic-Contaminated Soil","authors":"Zhen Zhang, Xinxin Fan, Rumeng Zhang, Xinghui Pan, Xuexue Zhang, Yi Ding, Ying Liu","doi":"10.1016/j.jhazmat.2025.138530","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.138530","url":null,"abstract":"Plastic pollution poses significant ecological and health risks. In this study, we enriched microbial consortia from plastic-contaminated soil capable of degrading low-density polyethylene (LDPE) film over a 28-day incubation period. Using two kinds of enriched cultures, the mean film weight loss rate (WLR) of 0.27 ± 0.04% (<em>p</em> < 0.01) was 9 times higher than the control. Scanning electron microscopy (SEM) revealed a average hole occurrence area of 0.67 ± 0.11 μm<sup>2</sup> in the topmost sample, while the control had no change. Fourier transform infrared (FTIR) revealed specific changes in hydrophilicity (increased by 5.70 ± 0.02 times) and crystallinity (decreased by 15.73 ± 3.26%). Meanwhile, FTIR analyses including peak occurrence at 3,741<!-- --> <!-- -->cm<sup>-1</sup>, carbonyl index and Lambert-Beer law calculations revealed moisture infiltration and predominant aldehyde carbonyl formation (88.69% in total carbonyl). The results of high-throughput sequencing indicated <em>Brevibacillus</em>, <em>Bacillus</em> and <em>Sporosarcina</em> were dominate genera in the mixed-cultures, and PICRUSt2 implied they could use LDPE as the sole carbon source. Our study aims to provided theoretical basis driving plastic degradation and to mitigate plastic pollution based on microbial resource development.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"30 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Martina Švábová, Marek Šváb, Maryna Vorokhta, Michael Pohořelý
{"title":"Evaluation of micropollutant removal from artificially recharged water using activated carbon","authors":"Martina Švábová, Marek Šváb, Maryna Vorokhta, Michael Pohořelý","doi":"10.1016/j.jhazmat.2025.138504","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.138504","url":null,"abstract":"Pilot scale micropollutant removal onto three types of granular activated carbon was tested over a period of 25 months. The columns were operated in a mode as similar as possible to the functioning of the main waterworks with the emphasis on minimizing the washing of the activated carbon layers. Sampling was performed monthly and a total of 222 micropollutants were evaluated. The columns were located at a waterworks in central Bohemia (Czech Republic). Real influent water from the river Jizera after artificial recharge and sand filtration was used. The aim of this study was to verify whether the process of micropollutant removal was suitable under the operating conditions at the waterworks.Clear seasonal patterns of micropollutant concentrations in the influent were detected. All three columns were able to effectively remove or substantially decrease the concentration of most micropollutants. The best performance was shown for the activated carbon Aquasorb 5005, which had the highest surface area and a well developed porous structure with the highest proportion of mesopores. The data were evaluated in order to identify micropollutant parameters important for their removal, and the pH-dependent octanol/water partition coefficient was identified as the key parameter. Other important parameters were charge and polar surface area of the micropollutant molecules.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"95 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rui Zhou, Jian Chen, Haobo Bian, Lu Li, Tao Zhang, Lei Liao, Haobo Niu, Leyi Yin, Guoxin Huang
{"title":"A framework for spatial correlations between industrial pollution sources and groundwater vulnerabilities based on machine learning and spatial cluster analysis: implications for risk control","authors":"Rui Zhou, Jian Chen, Haobo Bian, Lu Li, Tao Zhang, Lei Liao, Haobo Niu, Leyi Yin, Guoxin Huang","doi":"10.1016/j.jhazmat.2025.138492","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2025.138492","url":null,"abstract":"The efficacy of groundwater pollution risk control is often limited by a lack of information on spatial correlations between industrial pollution sources and groundwater vulnerabilities. To overcome this limitation, a novel data-driven framework was established to reveal the spatial correlations between industrial pollution sources and groundwater vulnerabilities in Guangdong Province, China, mainly using a combination of genetic algorithm (GA), back propagation neural network (BPNN), kernel density estimation (KDE) and bivariate local Moran’s I (BLMI). The subjectivity in the indicator weighting of a DRASTICL model was successfully reduced by GA-BPNN, and then the groundwater vulnerability map was created by the GA-BPNN-DRASTICL model. A smoother surface of the industrial pollution source distribution was produced by KDE. The spatial clustering map between industrial pollution sources and groundwater vulnerabilities was generated by BLMI, explicitly showing their distribution characteristics and implying that the specific measures should be taken for controlling risks of groundwater pollution in the different parts of the study area.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"47 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An AuNP-based indicator for rapid on-site detection of strontium ion","authors":"Zhen Dong , Yanying Wang , Xianming Li , Peng Wu","doi":"10.1016/j.jhazmat.2025.138525","DOIUrl":"10.1016/j.jhazmat.2025.138525","url":null,"abstract":"<div><div>Strontium (Sr) has attracted significant attention due to its radioactivity and physiological toxicity. Rapid and precise detection of Sr<sup>2</sup><sup>+</sup> was critical for risk mitigation, particularly in accidents involving strontium leaks. However, the existing methods often compromise accuracy, speed, and user-friendliness, making on-site detection of Sr<sup>2+</sup> challenging. Herein, we developed a gold nanoparticle (AuNP)-based indicator for Sr<sup>2+</sup> through leveraging high-affinity binding between Sr<sup>2+</sup> and G-quadruplex (G4) DNA to regulate the thioflavin T (ThT)-induced charge neutralization assembly of AuNPs. The proposed indicator achieved a detection limit of 2.5 nM, significantly lower than the maximum allowable concentration of Sr<sup>2+</sup> in drinking water (17.1 μM). To enable on-site detection of Sr<sup>2+</sup>, we designed a self-constructed portable suitcase housing all necessary components of the indicator (∼0.14 $ per test), and a smartphone app for colorimetric analysis. The proposed indicator was successfully explored for on-site detection of Sr<sup>2+</sup> in rivers across Chengdu, Kunming, and Xishuangbanna, China, demonstrating its reliability for environmental monitoring.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"494 ","pages":"Article 138525"},"PeriodicalIF":12.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nourin I. Seenthia , Wael Abdelraheem , Samuel A. Beal , Joseph J. Pignatello , Wenqing Xu
{"title":"Simultaneous adsorption and hydrolysis of insensitive munition compounds by pyrogenic carbonaceous matter (PCM) and functionalized PCM in soils","authors":"Nourin I. Seenthia , Wael Abdelraheem , Samuel A. Beal , Joseph J. Pignatello , Wenqing Xu","doi":"10.1016/j.jhazmat.2025.138501","DOIUrl":"10.1016/j.jhazmat.2025.138501","url":null,"abstract":"<div><div>Military training with insensitive munitions (IM) deposits a large amount of post-detonation residues on range soils that may contaminate groundwater. This study investigated the effectiveness of pyrogenic carbonaceous matter (PCM) and functionalized PCM as a soil amendment to retain and transform IM compounds (e.g., 5-nitro-1,2-dihydro-3H-1,2,4-triazin-3-one (NTO), nitroguanidine (NQ), and 2,4-dinitroanisole (DNAN)). Initial hydrolysis tests showed quaternary ammonium (QA)-modified PCM accelerated NQ hydrolysis, while NTO formed charge-assisted hydrogen bonds with PCM's surface groups instead of hydrolyzing. We amended three soils with a subset of PCM materials and monitored the removal of NTO, NQ, and DNAN under varying pH conditions (i.e., 8.0, 10.0, and 12.0) and amendment dosages (i.e., 0, 2, 3, and 5 % (w)) in batch reactors. We also assessed the reactivity and reusability of the best-performing PCM by consecutive addition of IM post-detonation residues. Our results indicate that PCM can significantly enhance the removal of NTO, NQ, and DNAN in soils by 50–300-fold compared to soil controls, and QA-modified activated carbon (AC) performed the best. Increasing pH from 8.0 to 10.0 enhanced the DNAN hydrolysis with PCM, whereas NQ hydrolysis only occurred at pH 12.0. However, increasing PCM dosage from 2 % to 5 % (w) did not significantly increase the removal of NTO, NQ, and DNAN. QA-modified AC maintained its reactivity over three consecutive additions of IM post-detonation residues at pH 10.0, with an average NTO and NQ removal of 98.8 ± 0.6 % and 84.2 ± 3.2 %, respectively, and DNAN decay of 55.3 ± 2.9 %. Overall, our findings highlight the effectiveness of the functionalized PCM in simultaneously retaining and transforming IM compounds, providing a strategy for using reactive amendment <em>in-situ</em> to sustain military operation and mitigate pollution.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"494 ","pages":"Article 138501"},"PeriodicalIF":12.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Min Sung Kim , Seulgidaun Lee , Moonhee Park , Hyemi Jang , Mira Choi , Ji Yi Lee , Mijung Song , Changhyuk Kim , Junyoung Ahn , Zhijun Wu , Amgalan Natsagdorj , Jungju Seo , Tae Kwon Lee , Young Hwan Kim , Kyoung-Soon Jang
{"title":"Region-specific characterization and ecotoxicity assessment of PAH compounds in winter PM2.5 from three capital cities in Northeast Asia","authors":"Min Sung Kim , Seulgidaun Lee , Moonhee Park , Hyemi Jang , Mira Choi , Ji Yi Lee , Mijung Song , Changhyuk Kim , Junyoung Ahn , Zhijun Wu , Amgalan Natsagdorj , Jungju Seo , Tae Kwon Lee , Young Hwan Kim , Kyoung-Soon Jang","doi":"10.1016/j.jhazmat.2025.138536","DOIUrl":"10.1016/j.jhazmat.2025.138536","url":null,"abstract":"<div><div>Industrialization and urbanization in Northeast Asia have heightened PM<sub>2.5</sub> pollution, posing significant public health risks. This study examined the spatial and chemical variability of polycyclic aromatic hydrocarbons (PAHs) and their ecotoxicity in winter PM<sub>2.5</sub> from three capitals—Ulaanbaatar (UB, Mongolia), Beijing (BJ, China), and Seoul (SE, South Korea)—using two-dimensional gas chromatography time-of-flight mass spectrometry (GC×GC-TOF MS). PM<sub>2.5</sub> samples collected between December 15, 2020 and January 14, 2021 revealed UB had the highest concentrations (85.7 ± 36.7 μg m<sup>−3</sup>) and PAH levels (758.9 ± 224.7 ng m<sup>−3</sup>), primarily from coal combustion and biomass burning. BJ (30.3 ± 16.9 μg m<sup>−3</sup>; 41.4 ± 18.4 ng m<sup>−3</sup>) and SE (26.0 ± 14.4 μg m<sup>−3</sup>; 6.2 ± 2.4 ng m<sup>−3</sup>) had lower PAH levels but a higher share of secondary products, including oxygenated (OPAHs) and nitrogen-containing PAHs (NPAHs). Overall, 646 PAH compounds were identified: UB was dominated by methylated alkyl and sulfur-containing PAHs, while BJ and SE had more hydroxylated and carbonylated PAHs. QSAR ecotoxicity analysis indicated the highest toxicity in SE from hydroxylated PAHs and a broader toxic range in UB. These findings support air quality strategies to reduce coal combustion in UB and secondary PAH formation in BJ and SE.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"494 ","pages":"Article 138536"},"PeriodicalIF":12.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Runze Liu , Xiaokang Guo , Guangjian Yang , Shuangqing Lu , Feihu Chen , Wenxiao Jia , Ji Li , Jiling Niu , Hongbo Guo , Hui Zhu
{"title":"Formation of metal-microplastic complexes in lung adenocarcinoma is associated with increased risk of cancer progression","authors":"Runze Liu , Xiaokang Guo , Guangjian Yang , Shuangqing Lu , Feihu Chen , Wenxiao Jia , Ji Li , Jiling Niu , Hongbo Guo , Hui Zhu","doi":"10.1016/j.jhazmat.2025.138517","DOIUrl":"10.1016/j.jhazmat.2025.138517","url":null,"abstract":"<div><div>Metal-microplastic complexes (m-MPs) represent an emerging environmental health concern. However their presence and pathological implications in lung adenocarcinoma (LUAD) remain underexplored. This study employed a multimodal approach to characterize m-MPs in 15 LUAD patients, integrating several techniques, including laser direct infrared imaging (LDIR), pyrolysis gas chromatography-mass spectrometry (Py-GCMS), inductively coupled plasma analysis (ICP), and Sequencing at the transcriptome level. Totally, 34 distinct microplastic types were identified in lung tissues, with polyvinyl chloride (PVC) predominant in tumor tissues. Notably, the levels of aluminum (Al) and calcium (Ca) exhibited strong positive correlations with microplastics (MPs) content in tumors (<em>p</em> < 0.05). In this study, a novel approach was applied to assess the influences of m-MPs on tumor, through which we found that m-MPs accumulation could activate pro-tumorigenic pathways, leading to reduced overall survival (HR=1.59, <em>p</em> = 0.002) and disease-specific survival (HR=1.64, <em>p</em> = 0.01). Moreover, SYNE1 and RORA genes were identified as diagnostic/prognostic biomarkers for the assessment of m-MPs exposure in LUAD. Our findings revealed that m-MPs have the capacity for promoting metal ion deposition and oncogenic signaling, thereby involved in LUAD progression. This work also provides evidence using human tissue to demonstrate the association of m-MPs with lung cancer outcomes and advocate the development and application of therapeutic strategies targeting m-MPs.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"494 ","pages":"Article 138517"},"PeriodicalIF":12.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143915753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}