Journal of Hazardous Materials最新文献

{"title":"nLithium Nickel Cobalt Manganese Oxide Particles May Impair Cognitive Function via CTCF-associated Suppression of NMDAR-dependent Synaptogenesis","authors":"Xinchao Guan, Lijie Xie, Guoliang Li, Yujie He, Shilin Chen, Han Wang, Wenke Yuan, Huan Gao, Ming Chen, Xiaoge Yang, Yingxia Luo, Xiaojian Shao, Wen Chen, Lili Liu, Qing Wang","doi":"10.1016/j.jhazmat.2026.143790","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2026.143790","url":null,"abstract":"Lithium nickel cobalt manganese oxide (NCM) is the predominant cathode material for lithium-ion batteries (LIBs); however, its occupational health risks remain insufficiently understood. In this study, we found that in occupationally NCM‑exposed workers, urinary levels of Li, Ni, Co, and Mn were negatively associated with Mini‑Cog test scores and positively associated with serum neurofilament light chain (NfL, as neuronal injury marker). In an intratracheal instillation rat model, NCM-exposed rats exhibited learning and memory deficits, neuronal injury, and reduced synaptic complexity. RNA‑seq analysis revealed suppression of N-methyl-D-aspartate receptors (NMDAR)‑dependent synaptogenesis pathways in rat hippocampus, along with downregulation of NMDAR subunits (GluN2A and GluN2B) and postsynaptic proteins such as CaMKII‑α, MAP2, PSD‑95, and Synapsin‑1. In SH-SY5Y cells, treatment with NCM-derived metals recapitulated these gene expression changes. Bioinformatics analysis identified CCCTC-binding factor (CTCF) as a potential regulator of NMDAR. Cellular thermal shift assays (CETSA) identified the binding of NCM-derived metals to CTCF. Based on <ce:italic>in vivo</ce:italic> data, human equivalent concentrations (HECs) derived from BMDL<ce:inf loc=\"post\">10</ce:inf> of Ni and Mn were below the current OELs of China and America. Collectively, these findings indicate that NCM may induce neurotoxicity and impair learning and memory by disrupting synaptogenesis, and CTCF is implicated in this process.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"10 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149087365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning-assisted ratiometric Zr-MOF-based fluorescent probe for intelligent detection of uranyl ions","authors":"Qing Wang, Haixia Quan, Peipei Liao, Meifeng Li, Yonggang Lv","doi":"10.1016/j.jhazmat.2026.143787","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2026.143787","url":null,"abstract":"Uranium contamination in aquatic environments poses potential threats to ecosystems and human health due to the high radioactive and chemical toxicity of uranyl ions (UO<ce:inf loc=\"post\">2</ce:inf><ce:sup loc=\"post\">2+</ce:sup>), necessitating the development of accurate and rapid detection strategies for the monitoring of UO<ce:inf loc=\"post\">2</ce:inf><ce:sup loc=\"post\">2+</ce:sup>. Herein, a zirconium-based metal-organic framework (NH<ce:inf loc=\"post\">2</ce:inf>-PCN-134) was constructed through a one-pot hydrothermal method and employed as a ratiometric fluorescent probe for the detection of UO<ce:inf loc=\"post\">2</ce:inf><ce:sup loc=\"post\">2+</ce:sup>. The ratiometric fluorescence response of NH<ce:inf loc=\"post\">2</ce:inf>-PCN-134 toward UO<ce:inf loc=\"post\">2</ce:inf><ce:sup loc=\"post\">2+</ce:sup> featured emission quenching at 655<ce:hsp sp=\"0.25\"></ce:hsp>nm and enhancement at 515<ce:hsp sp=\"0.25\"></ce:hsp>nm, enabling quantitative analysis based on the I<ce:inf loc=\"post\">515</ce:inf>/I<ce:inf loc=\"post\">655</ce:inf> intensity ratio. Furthermore, the probe exhibited a broad linear response range of 0–200<ce:hsp sp=\"0.25\"></ce:hsp>μM with a low detection limit of 1.23<ce:hsp sp=\"0.25\"></ce:hsp>μM, and excellent selectivity toward UO<ce:inf loc=\"post\">2</ce:inf><ce:sup loc=\"post\">2+</ce:sup> in the presence of various interfering ions. The ratiometric fluorescence response was attributed to the synergistic effects of photoinduced electron transfer (PET) and molecular interactions between NH<ce:inf loc=\"post\">2</ce:inf>-PCN-134 and UO<ce:inf loc=\"post\">2</ce:inf><ce:sup loc=\"post\">2+</ce:sup>. Moreover, the color features extracted from fluorescent images were integrated with multiple machine learning models for quantitative prediction, which exhibited strong agreement between the predicted and experimental values even in the presence of interfering ions, demonstrating their effectiveness for quantitative analysis. This study provides a machine learning-assisted ratiometric fluorescence sensing strategy for intelligent detection of UO<ce:inf loc=\"post\">2</ce:inf><ce:sup loc=\"post\">2+</ce:sup>, highlighting its potential for environmental monitoring.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"9 4 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149087314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyaniline-modified sea sand suppresses phase-change-driven microplastic transport via regenerable interfacial retention for long-term solar desalination","authors":"Lin Gong, Xushen Liu, Jingzhe Li, Shengde Yu, Wei Zhang","doi":"10.1016/j.jhazmat.2026.143775","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2026.143775","url":null,"abstract":"Solar interfacial evaporation (SIE) provides a low-carbon route for seawater desalination, but its nonequilibrium phase-change interface may facilitate microplastic transfer from seawater to condensate. To mitigate this water-quality risk, a low-cost, regenerable photothermal interface was constructed by modifying natural sea sand with polyaniline (PANI@SS). The PANI coating enhanced broadband solar absorption and promoted interfacial water activation, achieving an evaporation rate of 2.20<ce:hsp sp=\"0.25\"></ce:hsp>kg·m−2·h−1. Adsorption, spectroscopy, thermodynamic analysis, and molecular dynamics simulations showed that PANI converted the weak physical retention of pristine sea sand into stable interfacial anchoring, counteracting phase-change-driven microplastic transport. Reversible protonation/deprotonation enabled pH-regulated interfacial affinity, supporting facile regeneration and sustained antifouling performance. PANI@SS effectively retained seven representative microplastics with different structures and polarities, indicating broad applicability. In a 30-day outdoor test using natural seawater from the Yellow, Yangtze, and Pearl River estuaries, cumulative freshwater production exceeded 220<ce:hsp sp=\"0.25\"></ce:hsp>kg·m−2, while major ion concentrations met drinking-water guideline limits. Evaporation performance, microplastic retention, and material structure remained stable after repeated evaporation–regeneration cycles. This study reveals the competition between phase-change transport and interfacial retention as a key mechanism governing microplastic migration and product-water safety in SIE, providing a practical strategy for efficient, durable, and regenerable solar desalination.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"23 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149087351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation-dependent pulmonary disposition and secondary hepatic responses of the household insecticide hydramethylnon following respiratory tract delivery","authors":"Su Hwan Park, Jaebaek Jang, So-Young An, Hye-Jin Jeong, Dong Yeop Kim, Jung Eun Park, Min-Seok Kim, Jiyoung Jeong, Mi Hye Kwon, Seungbum Choi, Se Jong Oh, Yong Joo Park, Dong Hyun Kim, Jongho Jeon, Jong-Ho Lee, Wan-Seob Cho","doi":"10.1016/j.jhazmat.2026.143773","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2026.143773","url":null,"abstract":"Hydramethylnon (HM) is a household-use insecticide whose inhalation hazard remains poorly characterized. We combined whole-body disposition analysis with mechanistic assessment in pulmonary and hepatic systems. Structurally identical <ce:sup loc=\"post\">18</ce:sup>F-labeled HM enabled dynamic positron emission tomography/computed tomography after intratracheal instillation in mice. Pulmonary clearance was rapid, with predominant redistribution to the liver and a fourfold longer half-life for the polyethylene glycol (PEG)-based than the ethanol-based dispersion (26.92 versus 6.59<ce:hsp sp=\"0.25\"></ce:hsp>min). In an air–liquid interface model, HM was cytotoxic to Calu-3 and H441 epithelial cells, whereas barrier-related responses reflected both PEG and HM in Calu-3 cells but predominantly HM in H441 cells. In HepG2 hepatocytes, differentiated THP-1 macrophages, and primary alveolar macrophages, HM induced apoptosis, adenosine triphosphate depletion, mitochondrial loss, impaired respiration, and reactive oxygen species generation without altering glycolysis. <ce:italic>In vivo,</ce:italic> hepatic apoptosis increased after both three-dose intratracheal instillation and 28-day repeated pharyngeal aspiration, whereas reduced hepatic mitochondrial content and elevated serum transaminases emerged only after 28 days, indicating duration- and cumulative dose-dependent hepatic responses. This study identifies the liver as the principal secondary exposure compartment after respiratory delivery of HM and shows that formulation modifies pulmonary retention, providing route-specific disposition data for hazard identification rather than quantitative risk assessment.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"8 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149087352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced removal of sulfamethoxazole by tourmaline-modified iron‑carbon substrate in constructed wetlands and underlying microbial metabolic mechanisms","authors":"Junchi Si, Yahui Li, Junyong He, Yulian Li, Peidong Hong, Chao Xie, Zijian Wu, Jiandong Lu, Dandan Yang, Lei Meng, Lingtao Kong","doi":"10.1016/j.jhazmat.2026.143783","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2026.143783","url":null,"abstract":"Tourmaline-modified iron‑carbon (Fe‑C) composite fillers were fabricated via high-temperature sintering, and their structural, electrochemical, pollutant removal and microbial response characteristics were systematically investigated. The optimal formulation was 15<ce:hsp sp=\"0.25\"></ce:hsp>wt% tourmaline with a 3:1 Fe‑C mass ratio. The optimal filler features a hierarchical porous structure, and tourmaline's spontaneous polarization mitigates iron passivation and enhances interfacial electron transfer. Static adsorption experiments demonstrated that sulfamethoxazole (SMX) adsorption onto the filler is dominated by monolayer chemisorption, with a maximum capacity of 2.91<ce:hsp sp=\"0.25\"></ce:hsp>mg/g. In a 21-day high-load static simulation system, the optimal filler achieved removal efficiencies of 84.2% for chemical oxygen demand (COD), 72.3% for total nitrogen (TN), 68.7% for total phosphorus (TP) and 84.8% for SMX, significantly outperforming pure Fe‑C, commercial Fe‑C and gravel controls. Metagenomic analysis revealed that tourmaline modification was associated with enrichment of denitrifying functional genera and key genes related to electron transport, denitrification, and SMX biodegradation. The synergistic mechanism enabled efficient pollutant removal, and continuous-flow verification over 77 days further confirmed the long-term stability of the modified filler (COD 90.8%, TN 93.3%, TP 83.3%, SMX 90.8%), demonstrating its practical potential for treating antibiotic-containing agricultural wastewater with low C/N ratios (approximately 3:1 in this study).","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"8 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149087391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic Release of Phthalate Esters from Pristine and Aged Microplastics in Indoor Environments and Their Potential Lung Health Impacts","authors":"Liyuan Peng, Na Zheng, Siyu Sun, Qirui An, Changcheng Chen, Xiaoqian Li, Qiuyan Lin, Zhifei Xiu","doi":"10.1016/j.jhazmat.2026.143788","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2026.143788","url":null,"abstract":"Microplastics (MPs) in indoor environments are a significant source of phthalate ester (PAEs) release, posing potential health risks. This study investigated PAEs release kinetics from polyethylene (PE) and polylactic acid (PLA) in indoor air, and from four common MPs (polyamide, polycarbonate, polyethylene, and polylactic acid) at multiple aging stages (0, 7, 14, 21, and 28 days) in simulated lung fluids (ALF and GS) through simulation experiments. Results demonstrated that light, temperature, and aging degree significantly influence PAEs release. Aging altered surface properties (increased specific surface area and hydrophilicity), causing non-monotonic release trends of PAEs. Both light exposure and elevated temperature significantly promoted PAEs release (<ce:italic>p</ce:italic> &lt; 0.05), with kinetics following a pseudo-second-order model, governed by surface desorption and intraparticle diffusion. PAEs release was significantly higher in ALF than in GS (<ce:italic>p</ce:italic> &lt; 0.05). Aged MPs exhibited elevated relative concentrations of ·OH and ·O₂⁻ in lung fluids, indicating a potential oxidative burden. This study elucidates the dynamic release behavior of additives during aging of microplastics, and highlights the dynamic aging process of MPs as well as the potential health impacts.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"35 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149087361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The GmEIN3.1-GmERF3-GmABCI17 module coordinately regulates soybean root development and cadmium tolerance through the ethylene signaling","authors":"Chen Liu, Zhenghao Wang, Zhuorui Zhang, Songjie Qi, Min Wang, Xinchen Wang, Yubo Jia, Xinxia Li, Hengxi Zhao, Yaqing Wei, Huiru Sun, Hanqiu Zhang, Qiumin Chen, Zhimin Dong","doi":"10.1016/j.jhazmat.2026.143781","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2026.143781","url":null,"abstract":"Soybean (<ce:italic>Glycine max</ce:italic>) is a critical oil crop and a key source of fat and protein for both humans and animals. Cadmium (Cd) stress is a severe heavy metal stresses limiting root growth and yield. Significant variations exist in Cd tolerance among different soybean varieties. However, the molecular mechanisms underlying Cd-induced root growth inhibition and Cd accumulation remain elusive. Here, we identified that the GmEIN3.1-GmERF3-GmABCI17 module in the ethylene signal transduction pathway coordinately regulates soybean root growth and Cd accumulation. We found Cd stress positively regulates the expression of <ce:italic>GmEIN3.1</ce:italic> (Glyma.13G076700), which inhibits root development but reduces Cd accumulation and enhances Cd tolerance. Multiple lines of experimental evidence demonstrate that GmEIN3.1 represses the expression of <ce:italic>GmERF3</ce:italic> (Glyma.14G050100), a transcription factor that promotes root development. In turn, GmERF3 binds to and suppresses the expression of the ABC transporter <ce:italic>GmABCI17</ce:italic> (Glyma.08G055000), a Golgi-localized protein with Cd efflux transporter activity. Overexpression of <ce:italic>GmABCI17</ce:italic> promotes Cd efflux, thereby reducing intracellular Cd accumulation and enhancing Cd tolerance. Collectively, the GmEIN3.1-GmERF3-GmABCI17 transcriptional cascade coordinates Cd-induced root growth inhibition, restricts Cd accumulation, and Cd tolerance in soybean. It provides exploitable targets for breeding low-Cd soybean cultivars and addresses critical food safety concerns.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"53 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149087392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salinity–Regulated Interfacial Interactions of Asphaltene Subfractions: Mechanistic Insights into Petroleum Emulsion Stability and Destabilization","authors":"Hongtao Ma, Xiaohui Mao, Yuanyuan Wang, Chenyu Qiao, Yongxiang Sun, Ying Hu, Hao Zhang, Hongbo Zeng","doi":"10.1016/j.jhazmat.2026.143770","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2026.143770","url":null,"abstract":"Asphaltenes are problematic petroleum components that contribute to persistent oil–water emulsions and complicate produced water treatment. However, how asphaltene subfractions with distinct interfacial activities respond to brine salinity and regulate emulsion stability remains insufficiently understood. Here, the salinity-dependent adsorption, interfacial film formation, and intermolecular interactions of interfacially active asphaltenes (IAA) and interfacially non-active asphaltenes (INAA) were investigated at different NaCl concentrations using dynamic interfacial tension measurements, interfacial film characterization, surface force measurements, and emulsion stability tests. With increasing NaCl concentration, the extrapolated equilibrium interfacial tension of INAA-containing systems increased monotonically, whereas that of IAA-containing systems initially decreased and then increased. Apparent diffusion coefficients and crumpling ratios increased with NaCl concentration up to 1<ce:hsp sp=\"0.25\"></ce:hsp>M and decreased at 3<ce:hsp sp=\"0.25\"></ce:hsp>M, whereas compression energies remained elevated or increased further at 3<ce:hsp sp=\"0.25\"></ce:hsp>M. Droplet adhesion increased at 1–100<ce:hsp sp=\"0.25\"></ce:hsp>mM NaCl but declined sharply at 1–3<ce:hsp sp=\"0.25\"></ce:hsp>M. Bottle tests using a steam-assisted gravity drainage emulsion showed greater stability at low salinity and pronounced oil–water separation at high salinity. These results reveal a salinity-dependent transition from interfacial film strengthening to emulsion destabilization, providing mechanistic insights into the salinity-regulated interfacial interactions of asphaltene subfractions in stabilizing petroleum emulsions.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"23 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149087396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FTO mediates benzo[a]pyrene-induced lung cancer progression through regulating ferroptosis via lactylation-driven YTHDF2-KEAP1-NRF2 axis","authors":"Na Wang, Wan-tao Peng, Ling-qiao Wang, Hong-qiang Chen, Yong Zeng, Yu Shi, Zhe Zhang, Jiang-ying Li, Shi-meng Zhou, Qing-qing Liu, Shuang-wu Deng, Xue Han, Zi-yuan Zhou, Mao-lin Yao, Wen-bin Liu","doi":"10.1016/j.jhazmat.2026.143786","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2026.143786","url":null,"abstract":"N6-methyladenosine (m<ce:sup loc=\"post\">6</ce:sup>A) RNA modification and histone lactylation are important regulators of gene expression and cancer progression, but their interplay in lung cancer associated with benzo[a]pyrene (BaP) remains unclear. Integrating clinical bioinformatics with cellular and in vivo analyses, we found that BaP enhanced migration and invasion while inducing a ferroptosis-resistant phenotype in lung cancer cells. Pharmacological modulation with Erastin and Ferrostatin-1 further supported ferroptosis involvement. BaP decreased FTO and increased YTHDF2 expression. FTO overexpression and YTHDF2 knockdown enhanced ferroptosis-associated lipid oxidation and suppressed migration and invasion, while their combined intervention attenuated BaP-associated experimental metastatic progression in vivo. Mechanistically, FTO overexpression reduced m<ce:sup loc=\"post\">6</ce:sup>A enrichment on <ce:italic>KEAP1</ce:italic> mRNA, whereas YTHDF2 associated with <ce:italic>KEAP1</ce:italic> transcripts and accelerated their decay. Disruption of the A2108 RRACH motif attenuated YTHDF2-responsive repression of the <ce:italic>KEAP1</ce:italic> reporter. KEAP1 knockdown attenuated FTO effects under BaP exposure. Upstream analyses supported AHR-dependent FTO regulation and a glycolysis-associated lactate–H3K18la contribution to YTHDF2 upregulation. Collectively, these findings support an m<ce:sup loc=\"post\">6</ce:sup>A-sensitive FTO/YTHDF2–KEAP1–NRF2 framework contributing to ferroptosis defense and BaP-associated malignant progression.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"13 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149087388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc differentially modulates resistome responses to ciprofloxacin and sulfamethazine in activated sludge","authors":"Yongchao Li, Yu Ang Li, Shijie Dong, Jiangxiao Luo, Shixuan Yu, Wenhui Xie, Bin Shi, Renxin Zhao","doi":"10.1016/j.jhazmat.2026.143785","DOIUrl":"https://doi.org/10.1016/j.jhazmat.2026.143785","url":null,"abstract":"Antibiotics and metals frequently coexist in wastewater, but whether metals uniformly intensify antibiotic-driven resistome selection remains unclear. Here, activated sludge reactors were exposed to zinc (Zn), ciprofloxacin (CIP), sulfamethazine (SMZ), and their combinations across four stepwise exposure stages. Read-based, assembly-based, and genome-resolved metagenomics were integrated to characterize antibiotic resistance gene (ARG) abundance, composition, mobility-associated genetic contexts, and putative hosts. Antibiotics primarily drove resistome restructuring, whereas Zn alone had limited effects. CIP strongly enriched ARGs, while Zn co-exposure attenuated this response and caused late-stage declines in total ARG abundance and the relative abundance of ARG-hosting metagenome-assembled genomes (MAGs). In contrast, ARG abundance remained comparatively stable under SMZ but increased progressively under Zn+SMZ. Because antibiotic concentrations increased sequentially over time, these patterns represent stage-dependent responses rather than independent concentration effects. Antibiotic-related treatments showed higher contributions of ARGs on predicted plasmid-like contigs and more frequent ARG–mobile genetic element co-localization. Pseudomonadota dominated putative ARG hosts, and CIP showed broader ARG–host profiles. Approximately 67% of ARG-hosting MAGs carried virulence factor genes, indicating co-occurring resistance and virulence-associated genomic potential. These findings demonstrate that Zn altered activated sludge resistome in an antibiotic-dependent manner, highlighting that mixture effects cannot be inferred from single-contaminant responses alone.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"32 1","pages":""},"PeriodicalIF":13.6,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149087390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}