Medicine in Microecology最新文献_第8页

Intestinal mucosal and fecal microbiota profiles in Crohn's disease in Chinese children 中国儿童克罗恩病的肠粘膜和粪便微生物群特征

Medicine in Microecology Pub Date : 2023-03-01 DOI: 10.1016/j.medmic.2022.100071

Peilin Wu , Bin Wu , Zehao Zhuang , Junhong Liu , Linliang Hong , Bihong Ma , Biyun Lin , Junxi Wang , Chenye Lin , Junhong Chen , Suqing Chen

{"title":"Intestinal mucosal and fecal microbiota profiles in Crohn's disease in Chinese children","authors":"Peilin Wu , Bin Wu , Zehao Zhuang , Junhong Liu , Linliang Hong , Bihong Ma , Biyun Lin , Junxi Wang , Chenye Lin , Junhong Chen , Suqing Chen","doi":"10.1016/j.medmic.2022.100071","DOIUrl":"10.1016/j.medmic.2022.100071","url":null,"abstract":"<div><p>Imbalance in the microbiota has been identified in Crohn's disease (CD). We explored the difference of the microbiota in fecal and intestinal mucosa (including ileocecal junction, terminal ileum and transverse colon) in pediatric patients with active CD, CD patients in remission, nonspecific enteritis (NE) and the healthy children. Seven children had active CD, 5 cases of patients achieve remission (CDR), 19 were NE patients, and 11 were healthy controls (Col). A total of 168 samples were collected. Microbiota compositions were analyzed using 16 S rRNA sequencing. The results showed that, in fecal samples, <em>Clostridium_sensu_stricto, Enterobacter</em> and <em>Akkermansia</em> had a higher relative abundance in the CD group than that in CDR, Col and NE groups. <em>Fusobacterium</em> and <em>Streptococcus</em> showed a higher abundance in both CD and CDR than that in Col group. In intestinal mucosa samples, the bacterial communities of the three sampling sites were extremely similar. <em>Escherichia-Shigella</em> was the most abundant mucosal bacteria in the CD group, and the abundance of <em>Bacillus, Ruminococcus_torques_group, Streptococcus, Faecalibacterium</em> and <em>Blautia</em> was lower in the CD group than in other groups. In conclusion, as a very characteristic bacteria, the abundance of <em>Escherichia-Shigella</em> in the intestinal mucosa can be used as a diagnostic criterion for CD patients. Also, the <em>Bacilus</em> and <em>Blautia</em>, which were not as prominent as <em>Escherichia-Shigella</em> could still be used as a diagnostic candidate due to their neatness in CD patients. Mucosal samples may be better than stool samples when assessing the community and diversity of patients' intestinal microbes.</p></div>","PeriodicalId":36019,"journal":{"name":"Medicine in Microecology","volume":"15 ","pages":"Article 100071"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45711455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The association of HPV infection and vaginal microbiota of reproductive women in China: A multicenter cohort study protocol 中国育龄妇女HPV感染与阴道微生物群的关系:一项多中心队列研究方案

Medicine in Microecology Pub Date : 2023-03-01 DOI: 10.1016/j.medmic.2022.100072

Zuyi Zhou , Yi Hou , Wei Qing , Yiya Shi , Yingxuan Zhang , Rongdan Chen , Jinxia Ou , Hongwei Zhou , Muxuan Chen

{"title":"The association of HPV infection and vaginal microbiota of reproductive women in China: A multicenter cohort study protocol","authors":"Zuyi Zhou , Yi Hou , Wei Qing , Yiya Shi , Yingxuan Zhang , Rongdan Chen , Jinxia Ou , Hongwei Zhou , Muxuan Chen","doi":"10.1016/j.medmic.2022.100072","DOIUrl":"10.1016/j.medmic.2022.100072","url":null,"abstract":"<div><p>Cervical cancer is a global health concern. Persistent oncogenic human papillomavirus (HPV) infection is a prerequisite for the development of cervical cancer. Emerging research indicates that the vaginal microbiota may play both protective and harmful roles in the acquisition and persistence of HPV and the development of cervical cancer. This multicenter cohort study mainly aims to reveal the association of vaginal microbiota with the outcome of HPV infection in six months in women of reproductive age. We will recruit 50 research centers and enroll a total of about 10,000 female volunteers with a series of strict inclusion and exclusion criteria across China, including HPV positive and HPV negative women. A unified questionnaire will be used to obtain the sociodemographic information, clinical data and lifestyle of all volunteers. Specimens including vaginal secretions swabs and cervical exfoliated cells will be collected at the first visit and follow-up after six months. We will use 16S rRNA sequencing to characterize the vaginal microbiota of volunteers’ vaginal swabs. Twenty-one HPV genotypes and other sexually transmitted pathogens, including <em>Neisseria gonorrhoeae</em>, <em>Chlamydia trachomatis</em>, <em>Ureaplasma urealyticum</em>, <em>Mycoplasma hominis</em>, <em>Mycoplasma genitalium</em> and <em>Herpes simplex virus</em> type Ⅱ will be examined using flow-through hybridization and gene chip. The association of HPV infection and vaginal microbiota will be investigated, and the core microbiota attributes to the persistent HPV infection will be analyzed in this study.</p></div>","PeriodicalId":36019,"journal":{"name":"Medicine in Microecology","volume":"15 ","pages":"Article 100072"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42376212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Meta-analysis of the gut microbiota alterations in patients with gastric cancer in China 癌症患者肠道微生物群变化的Meta-analysis分析

Medicine in Microecology Pub Date : 2023-03-01 DOI: 10.1016/j.medmic.2022.100069

Jinpu Yang , Yongqiang Si , Jingchen Zhang , Xia Liu , Jie Gao , Wenhui Lei , Feng Chen , Yiwen Cheng , Zongxin Ling

{"title":"Meta-analysis of the gut microbiota alterations in patients with gastric cancer in China","authors":"Jinpu Yang , Yongqiang Si , Jingchen Zhang , Xia Liu , Jie Gao , Wenhui Lei , Feng Chen , Yiwen Cheng , Zongxin Ling","doi":"10.1016/j.medmic.2022.100069","DOIUrl":"10.1016/j.medmic.2022.100069","url":null,"abstract":"<div><p>Gastric cancer (GC) is one of the most common types of cancer and continues to threaten human health. The microbiota plays an important role in health and disease, including GC. Dysbiosis of gut microbiota has been reported to be associated with various diseases. There are no published meta-analyses of gut microbiota alterations in patients with GC in China. A meta-analysis was performed by searching the PubMed, Web of Science, EMBASE, and Cochrane Library databases up to July 2022. Nine eligible studies, representing 405 patients, were included in the analysis. At the phylum level, Proteobacteria (mean difference 12.46 (3.06–21.87), p < 0.05) was significantly increased, and Firmicutes (mean difference −4.10 (−7.65 to −0.56), p < 0.05) was decreased in patients with GC. At the genus level, <em>Desulfovibrio</em> (mean difference 0.38 (0.16–0.59); p < 0.05) and S<em>treptococcus</em> (mean difference 1.92 (0.37–3.46), p < 0.05) were significantly increased in patients with GC. No significant difference was observed in gut microbial diversity between patients and non-GC controls. Subgroup analysis suggested that region, sample size, and quality of studies caused heterogeneity to different extents. In summary, our study indicated a difference in gut microbial composition between patients with GC and individuals without GC. No significant differences were observed in the diversity of the gut microbes. Changes in the gut microbiota of patients with GC could potentially be used for the non-invasive diagnosis of GC.</p></div>","PeriodicalId":36019,"journal":{"name":"Medicine in Microecology","volume":"15 ","pages":"Article 100069"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43921158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amniotic fluid and vaginal microbiota in pregnant women with gestational diabetes mellitus by metagenomics 宏基因组学研究妊娠期糖尿病孕妇羊水和阴道微生物群

Medicine in Microecology Pub Date : 2023-03-01 DOI: 10.1016/j.medmic.2022.100074

Wanting Zheng , Yuxin Huang , Dianjie Li , Dongmei Hu , Chunzhu Jin , Alena Sadykova , Wei Cai , Can Liao , Shilei Pan

{"title":"Amniotic fluid and vaginal microbiota in pregnant women with gestational diabetes mellitus by metagenomics","authors":"Wanting Zheng , Yuxin Huang , Dianjie Li , Dongmei Hu , Chunzhu Jin , Alena Sadykova , Wei Cai , Can Liao , Shilei Pan","doi":"10.1016/j.medmic.2022.100074","DOIUrl":"10.1016/j.medmic.2022.100074","url":null,"abstract":"<div><h3>Aims</h3><p>To compared the amniotic fluid and vaginal microbiota of pregnant women, who developed gestational diabetes mellitus to those who did not, and explored if any differences exist in the composition and functional genes of their amniotic fluid and vaginal microbiota.</p></div><div><h3>Methods</h3><p>We compared the amniotic fluid and vaginal microbiota of five GDM patients and five non-GDM pregnant women in the third trimester of pregnancy by using metagenomics, and analyzed the characteristics, functions, and differences between two groups.</p></div><div><h3>Results</h3><p>The analysis of the amniotic fluid showed significant differences in genus and species. In contrast, there was no significant difference in vaginal microbial community structure between two groups. The alpha diversity in the GDM group was higher than the control, but the difference was not statistically significant. There was a statistically significant difference in the species-level beta-diversity measured by Bray–Curtis distance of the amniotic fluid communities between two groups, while no significant differences were observed in the vaginal microbiota. Regarding the functions, we observed that the microbial communities of the amniotic fluid and vaginal secretions were involved in the regulation of a variety of host metabolic pathways, including carbohydrate metabolism, membrane transport, energy, amino acid metabolism, nucleotide metabolism, etc. No significant differences were observed between two groups, but different sites exist.</p></div><div><h3>Conclusion</h3><p>The amniotic fluid of this study was discovered to have a heterogeneous, albeit small in number, community of microorganisms.Our research suggests that gestational diabetes has a very limited impact on the amniotic fluid and vaginal microbiome. The composition and the functions of the microbiota at different sites are different.</p></div>","PeriodicalId":36019,"journal":{"name":"Medicine in Microecology","volume":"15 ","pages":"Article 100074"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47436622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Fungal composition in saliva and plaque in children with caries: Differences and influencing factors 龋齿儿童唾液和牙菌斑中真菌成分的差异及影响因素

Medicine in Microecology Pub Date : 2023-03-01 DOI: 10.1016/j.medmic.2023.100076

Meixiang Yin , Yang You , Xiao Zheng , Qiuying Liang , Buling Wu , Wen'an Xu

{"title":"Fungal composition in saliva and plaque in children with caries: Differences and influencing factors","authors":"Meixiang Yin , Yang You , Xiao Zheng , Qiuying Liang , Buling Wu , Wen'an Xu","doi":"10.1016/j.medmic.2023.100076","DOIUrl":"10.1016/j.medmic.2023.100076","url":null,"abstract":"<div><p>Caries is a dental disease that can affect oral and psychological health and has a high incidence in children. The role of fungal flora in childhood caries has not been fully described. In this study, we aimed to investigate the fungal composition differences and the influencing factors in unstimulated saliva (S) and supragingival plaque (P) samples in childhood caries. S and P samples were collected from 63 children with caries. The ITS2 region in the fungal genome was then amplified and sequenced. Subsequently, we quantified and analyzed the fungal compositions in the samples. <em>Cryptococcus</em> was the most abundant genus in the S and P subgroups. The relative abundances of <em>Cryptococcus</em> and <em>Wickerhamomyces</em> significantly differed between the S and P subgroups (<em>p</em> < 0.05). Significant differences were also observed in alpha and beta diversities between the two subgroups (<em>p</em> < 0.05). However, no significant differences were observed between the mycobiome of the SFe and SMa subgroups or the PFe and PMa subgroups (<em>p</em> > 0.05). Conversely, species differences were detected between the SDD and SMD subgroups (<em>p</em> < 0.05) but not in the PDD and PPD subgroups (<em>p</em> > 0.05). Our findings revealed significant differences in the mycobiome of unstimulated saliva and supragingival plaque. Dentition period and oral hygiene behaviors may have affected these differences.</p></div>","PeriodicalId":36019,"journal":{"name":"Medicine in Microecology","volume":"15 ","pages":"Article 100076"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42798586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First clinical case of VIM-1-producing Leclercia adecarboxylata: A case report and literature review VIM-1产生阿氏勒克莱尔的第一例临床病例报告及文献复习

Medicine in Microecology Pub Date : 2023-03-01 DOI: 10.1016/j.medmic.2022.100075

Mohammed Abdullah Al Shuhoumi , Abdulrahman Al Mhrooqi , Azza Al Rashdi , Rajesh Kumar , Ahood Al Jabri , Amal Al Kalbani , Amina Al Jardani

{"title":"First clinical case of VIM-1-producing Leclercia adecarboxylata: A case report and literature review","authors":"Mohammed Abdullah Al Shuhoumi , Abdulrahman Al Mhrooqi , Azza Al Rashdi , Rajesh Kumar , Ahood Al Jabri , Amal Al Kalbani , Amina Al Jardani","doi":"10.1016/j.medmic.2022.100075","DOIUrl":"10.1016/j.medmic.2022.100075","url":null,"abstract":"<div><p>Leclercia adecarboxylata is a recently acknowledged emerging pathogen. It is a member of the Enterobacterals family, formerly thought to be a member of the genus Escherichia. Isolation was reported from various animal and environmental specimens. However, it rarely causes infection in humans, and the true frequency is unknown or underestimated. Leclercia adecarboxylata showed an ascending resistance grade from pan-sensitive to Carbapenem-resistant due to its ability to produce and harbour hydrolysing enzymes that challenge daily clinical practices. In our report, the isolate was misidentified as Citrobacter koseri by Analytical Profile Index for Enterobacterals (API E), and as Pantoea species by Vitek 2 but confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S ribosomal RNA analysis as Leclercia adecarboxylata. Conventional PCR revealed the presence of two populations of resistance genes, VIM-1 and OXA-48. Herein, a report of the first clinical emergence of Leclercia adecarboxylata producing VIM-1 in a rectal swab of a 63-year-old non-immunocompromised female with acute intracerebral haemorrhage.</p></div>","PeriodicalId":36019,"journal":{"name":"Medicine in Microecology","volume":"15 ","pages":"Article 100075"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47480542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Inactivation of multiple human pathogens by Fathhome's dry sanitizer device: Rapid and eco-friendly ozone-based disinfection 通过Fathhome的干式消毒装置灭活多种人类病原体:快速、环保的臭氧消毒

Medicine in Microecology Pub Date : 2022-12-01 DOI: 10.1016/j.medmic.2022.100059

Ryan Kenneally , Quentin Lawrence , Ella Brydon , Kenneth H. Wan , Jian-Hua Mao , Subhash C. Verma , Amir Khazaieli , Susan E. Celniker , Antoine M. Snijders

{"title":"Inactivation of multiple human pathogens by Fathhome's dry sanitizer device: Rapid and eco-friendly ozone-based disinfection","authors":"Ryan Kenneally , Quentin Lawrence , Ella Brydon , Kenneth H. Wan , Jian-Hua Mao , Subhash C. Verma , Amir Khazaieli , Susan E. Celniker , Antoine M. Snijders","doi":"10.1016/j.medmic.2022.100059","DOIUrl":"10.1016/j.medmic.2022.100059","url":null,"abstract":"<div><p>SARS-CoV-2 spread rapidly, causing millions of deaths across the globe. As a result, demand for medical supplies and personal protective equipment (PPE) surged and supplies dwindled. Separate entirely, hospital-acquired infections have become commonplace and challenging to treat. To explore the potential of novel sterilization techniques, this study evaluated the disinfection efficacy of Fathhome's ozone-based, dry-sanitizing device by dose and time response. Inactivation of human pathogens was tested on non-porous (plastic) surfaces. 95.42–100% inactivation was observed across all types of vegetative microorganisms and 27.36% inactivation of bacterial endospores tested, with no residual ozone detectable after completion. These results strongly support the hypothesis that Fathhome's commercial implementation of gas-based disinfection is suitable for rapid decontamination of a wide variety of pathogens on PPE and other industrially relevant materials.</p></div>","PeriodicalId":36019,"journal":{"name":"Medicine in Microecology","volume":"14 ","pages":"Article 100059"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10347040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Meta-analysis of metagenomics reveals the signatures of vaginal microbiome in preterm birth 元基因组学的荟萃分析揭示了早产阴道微生物组的特征

Medicine in Microecology Pub Date : 2022-12-01 DOI: 10.1016/j.medmic.2022.100065

Yingfang Huo, Qingru Jiang, Wenjing Zhao

{"title":"Meta-analysis of metagenomics reveals the signatures of vaginal microbiome in preterm birth","authors":"Yingfang Huo, Qingru Jiang, Wenjing Zhao","doi":"10.1016/j.medmic.2022.100065","DOIUrl":"10.1016/j.medmic.2022.100065","url":null,"abstract":"<div><p>Preterm birth (PTB) is the main cause of perinatal incidence rate and mortality worldwide and seriously threatens lives of newborns. In order to improve the understanding of PTB etiology, this study aimed to investigate associations between vaginal microbiome and PTB. We conducted an in-depth meta-analysis on publicly available shotgun metagenomics datasets of vaginal microbiome, including comparisons of microbial composition, metabolic pathways, biosynthetic gene clusters (BGCs), and virulence factors (VFs) between PTB and Healthy groups. Our results showed that 113 species existed in PTB group and 161 species in Healthy group, and their species compositions were significantly different. PTB group was associated with six species, namely <em>Lactobacillus crispatus</em>, <em>Atopobium vaginae</em>, <em>Prevotella bivia</em>, <em>Neisseria subflava</em>, <em>Corynebacterium</em> sp. HMSC078H07 and <em>Capnocytophaga leadbetteri</em>. Between the two groups exhibited 314 significantly different KEGG orthologys. The distribution of BGCs in PTB group were significantly different from that in Healthy group. The total amount of BGCs in PTB group was 95 and they were divided into 12 types, in Healthy group 300 BGCs into 16 types. We also obtained 7080 types of VF genes in PTB group, and 10,748 in Healthy group. The virulence gene with the highest proportion in both groups was <em>ssrA</em>. To conclude, this meta-analysis indicated that significant differences of microbial relative abundance were observed between PTB and Healthy groups. PTB group carried less total amount and types of BGCs, and less types of VFs than those in Healthy group, and PTB group showed significantly different metabolic pathways from Healthy group. We also provided new hypotheses related to vaginal microbiome and PTB.</p></div>","PeriodicalId":36019,"journal":{"name":"Medicine in Microecology","volume":"14 ","pages":"Article 100065"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590097822000155/pdfft?md5=02127a396ad1f5a4b9929e2273986bae&pid=1-s2.0-S2590097822000155-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49535551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Westlake Gut Project: A consortium of microbiome epidemiology for the gut microbiome and health research in China 西湖肠道项目:中国肠道微生物组与健康研究的微生物组流行病学联盟

Medicine in Microecology Pub Date : 2022-12-01 DOI: 10.1016/j.medmic.2022.100064

Wanglong Gou , Yu-ming Chen , An Pan , Huijun Wang , Ke Zhang , Xiong-Fei Pan , Yan He , Yuanqing Fu , Zengliang Jiang , Zelei Miao , Chang Su , Jiguo Zhang , Wensheng Hu , Fang-fang Zeng , Wenjun Ma , Guo Cheng , Yimin Zhu , Hongwei Zhou , Bing Zhang , Ju-Sheng Zheng

{"title":"Westlake Gut Project: A consortium of microbiome epidemiology for the gut microbiome and health research in China","authors":"Wanglong Gou , Yu-ming Chen , An Pan , Huijun Wang , Ke Zhang , Xiong-Fei Pan , Yan He , Yuanqing Fu , Zengliang Jiang , Zelei Miao , Chang Su , Jiguo Zhang , Wensheng Hu , Fang-fang Zeng , Wenjun Ma , Guo Cheng , Yimin Zhu , Hongwei Zhou , Bing Zhang , Ju-Sheng Zheng","doi":"10.1016/j.medmic.2022.100064","DOIUrl":"10.1016/j.medmic.2022.100064","url":null,"abstract":"<div><p>Microbiome epidemiology is an emerging field for the discovery of novel disease biomarkers or intervention targets in human epidemiological studies. The determinants and consequences of human microbiome variations, especially for the gut microbiome, are both important and unsolved research questions, while the majority of findings of prior research are based on small-scale human studies with limited statistical power and a lack of replication/generalizability across different populations. Here, we initiated the Westlake Gut (WeGut) project, a consortium of gut microbiome-based human cohort studies in China. The WeGut project aims to provide a platform for the integration of gut microbiome data across different cohort studies, including two major components: 1) cohorts for healthy ageing and 2) cohorts for healthy pregnancy. The WeGut consortium includes seven core/foundation cohorts involving over 32,000 participants with gut microbiome and rich phenotype data across 17 provinces/megacities in China. Within the WeGut framework, we hope to disentangle the interplay among diet, lifestyle factors, host genetics and the gut microbiome in human health and explore the role of the gut microbiome for the precision prevention of chronic diseases in Chinese populations.</p></div>","PeriodicalId":36019,"journal":{"name":"Medicine in Microecology","volume":"14 ","pages":"Article 100064"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590097822000143/pdfft?md5=4dc5f94f259bdbb1915f342cae96aa7c&pid=1-s2.0-S2590097822000143-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43883884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Acute liver injury progression is associated with dynamic enteric eubiosis alteration in mice 小鼠急性肝损伤进展与动态肠道益生改变有关

Medicine in Microecology Pub Date : 2022-12-01 DOI: 10.1016/j.medmic.2022.100063

Fengyi Mei , Tao Chen , Xianglong Zhang , Peng Chen

{"title":"Acute liver injury progression is associated with dynamic enteric eubiosis alteration in mice","authors":"Fengyi Mei , Tao Chen , Xianglong Zhang , Peng Chen","doi":"10.1016/j.medmic.2022.100063","DOIUrl":"10.1016/j.medmic.2022.100063","url":null,"abstract":"<div><p>Liver health has long been linked to the homeostasis of gut microbiota. Although some studies have shown that alterations in the species and function of gut microbiota contribute to the initiation and development of acute liver injury (ALI), studies investigating the effects of ALI on gut microbial dynamic composition changes are still limited. To observe whether liver damage can alter the composition of gut microbiota dynamically, we established three chemical models (e.g., acetaminophen, carbon tetrachloride, lipopolysaccharide) of ALI. Using these models, multiple time points of liver injury and intestinal microbiome were analyzed through plasma biochemical analysis and 16S rRNA gene sequencing. We assessed α-diversity, Unifrac principal coordinates analysis (PCoA), and linear discriminant analysis effect size (LEfSe) in the injury and control groups. The composition of the gut microbiota underwent dramatic shifts with liver injury and recovery in each model. Additionally, specific microbial abundance was significantly correlated with the level of plasma alanine transaminase and aspartate transaminase. These data provide new evidence that liver dysfunction and restoration is dynamically linked with the changes in the intestinal microbiome.</p></div>","PeriodicalId":36019,"journal":{"name":"Medicine in Microecology","volume":"14 ","pages":"Article 100063"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590097822000131/pdfft?md5=d1fa0ca953dae709883367004cc1c170&pid=1-s2.0-S2590097822000131-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49246420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0