Yaoxue Xuebao Pub Date : 2017-03-01

Hui-yuan Bai, Shan Feng

{"title":"[Protection effects of schizandrin B against liver injury induced by clozapine in mice].","authors":"Hui-yuan Bai, Shan Feng","doi":"","DOIUrl":"","url":null,"abstract":"This study was conducted to test the effects of schizandrin B (Sch B) on clozapine (CLZ) induced\u0000chronic liver injury in mice and the mechanism of action, and this may provide a new approach for clinical\u0000prevention of CLZ-induced side effects. The CLZ was given to mice for three weeks alone or co-administration\u0000with Sch B. The changes of alanine aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase\u0000(ALP) and antioxidation indexes superoxide dismutase (SOD), malonic dialdehyde (MDA), glutathione (GSH)\u0000and liver histological evaluation were determined. Expression of Nrf2 was assayed in hepatic cells by immunohistochemical\u0000staining and Western blotting. The changes of relative gene expression of NAD(P)H: quinone\u0000oxidoreductase l (NQO1) and heme oxygenase 1 (HO-1) were assayed by real-time Q-PCR. The results showed\u0000that pretreatment with a lower dosage of Sch B (25, 50 mg·kg−1) prevented CLZ-induced liver injury as indicated\u0000by the reduced levels of ALT, AST and ALP, and the preserved activities of SOD, GSH and inhibiting MDA. It\u0000was shown that Sch B could up-regulate Nrf2 expression leading to nuclear accumulation of Nrf2 to induce\u0000oxidative response genes such as NQO1 and HO-1. These results suggest that Sch B could protect against liver\u0000injury induced by CLZ via the activation of the Nrf2/ARE signal pathway in a dose-dependent manner.","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36288710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Current status of ion channels as drug targets for diabetic neuropathic pain]. [离子通道作为糖尿病神经性疼痛药物靶点的现状]。

Yaoxue Xuebao Pub Date : 2017-03-01

Yu Zhou, Xiao-liang Wang, Hai-bo Yu

引用次数: 0

[Application of magnetic iron oxide nanoparticles in magnetic resonance / photothermal dual-modal imaging]. 磁性氧化铁纳米颗粒在磁共振/光热双峰成像中的应用

Yaoxue Xuebao Pub Date : 2017-03-01

Dan-hua Huang, Xue-yong Qi, Yan-ru Ge, Song Shen

{"title":"[Application of magnetic iron oxide nanoparticles in magnetic resonance / photothermal dual-modal imaging].","authors":"Dan-hua Huang, Xue-yong Qi, Yan-ru Ge, Song Shen","doi":"","DOIUrl":"","url":null,"abstract":"In this study, water-dispersible magnetic iron oxide (Fe3O4) nanoparticles were synthesized with\u0000solvothermal method. The nanoparticles were characterized with a transmission electron microscopy (TEM)\u0000and vibrating sample magnetometer (VSM). The in vitro magnetic resonance response and photothermal\u0000conversion characteristics of the nanoparticles were evaluated. In addition, the cellular uptake, cytotoxicity\u0000and biodistribution were studied. Finally, magnetic resonance/photothermal dual-modal imaging effect of\u0000the as-synthesized Fe3O4 nanoparticles was investigated in the tumor-bearing mice. The results showed that\u0000the obtained magnetic nanoparticles were uniform with a mean diameter of about 125 nm. Moreover, the\u0000superparamagnetic Fe3O4 nanoparticles showed remarkable magnetic resonance response and photothermal\u0000conversion properties. The results of cellular experiments showed that the cell viability was nearly 85% even the\u0000concentration of the nanoparticles was up to 1 000 μg·mL−1, an indicator of good biocompatibility. In addition,\u0000the nanoparticles could be taken up by the tumor cells and then located in the cytoplasm. After intravenous\u0000injection, the nanoparticles were tended to enrich in the tumor over time, which is helpful in achieving\u0000dual-modal magnetic resonance/photothermal imaging. In sum, the obtained Fe 3O4 nanoparticles showed great\u0000potential to be applied for multi-modal bio-imaging which may play an important role in the diagnosis of tumors.","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36290579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Advances and challenges in preclinical evaluation of therapeutic drugs for treating ischemic stroke]. [缺血性脑卒中治疗药物临床前评价的进展与挑战]。

Yaoxue Xuebao Pub Date : 2017-03-01

Ying Feng, Fei Ma, Ting-ting Xu, Shan Wang, Long-jian Huang, Xiao-ying Wang, Yong-jun Wang, Xiao-liang Wang

{"title":"[Advances and challenges in preclinical evaluation of therapeutic drugs for treating ischemic stroke].","authors":"Ying Feng, Fei Ma, Ting-ting Xu, Shan Wang, Long-jian Huang, Xiao-ying Wang, Yong-jun Wang, Xiao-liang Wang","doi":"","DOIUrl":"","url":null,"abstract":"Translating of scientific advances into clinical practice is a major challenge in the stroke research\u0000field in the past decades. There were many reasons involved: animal models might not accurately capture all\u0000aspects of clinical stroke in humans, the blind and randomized design principle was not closely followed, the\u0000inclusion and exclusion criteria was not previously established, sample size was inadequate, endpoint was not\u0000scientific nor blindly assessed, inadequate reporting of data and statistical flaws. To bridge the gap between\u0000experimental and clinical research, international consortia have attempted to establish standardized guidelines\u0000for study design and data report, which include optimizing animal models as well as experimental design, using\u0000innovative approaches to assess endpoint, making raw data and negative results available, establishing prior\u0000registration mechanism, conducting multicenter preclinical randomized controlled trials (pRCTs), systematic\u0000reviews and meta-analysis of preclinical studies, evolving the original focus on neuroprotection into a broader\u0000consideration of the role of neurovascular unit and ischemic cascade.","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36290153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Activating blood biological potency assay and chemical fingerprint chromatogram applied to quality evaluation of rhubarb]. 【活血生物效价法及化学指纹图谱法在大黄质量评价中的应用】。

Yaoxue Xuebao Pub Date : 2017-03-01

Hai-zhu Zhang, Peng Tan, Zhen-jie Liu, Jian Wang, Jia-bo Wang, Xiao-he Xiao

{"title":"[Activating blood biological potency assay and chemical fingerprint chromatogram applied to quality evaluation of rhubarb].","authors":"Hai-zhu Zhang, Peng Tan, Zhen-jie Liu, Jian Wang, Jia-bo Wang, Xiao-he Xiao","doi":"","DOIUrl":"","url":null,"abstract":"The biological potency assay and chemical fingerprint chromatogram were applied to quality\u0000evaluation of rhubarb. Using the biological potency as indicators, we evaluated the differences in quality of\u0000multiple batches of rhubarbs and related products. Using the platelet aggregation analyzer, we determined\u0000platelet aggregation rate in the different rhubarbs preparations, and calculated the biological potency based on the\u0000simplified probit principle. UPLC was adopted to establish the fingerprint spectra for rhubarbs. The spectral\u0000efficiency correlation analysis between chromatograms and biological potencies were conducted using the double\u0000variables of SPSS 22.0 software. We used three chemical composition to verify the potency. The biological\u0000potency results suggest that Rheum palmatum has a more potent activity than Rheum tanguticum, and\u0000wine-treated rhubarb had a higher potentcy than charred. We identified 10 elements in the Fingerprint Spectrum.\u0000The relevant elements including rhein-8-O-β-D-glucoside, emodin-8-O-β-D-glucoside and rhein have the\u0000strongest activity in the inhibition of platelet aggregation. In conclusion, this study provides a analytical\u0000method for rhubarb biological potency based on determination of the maximum antagonism rate model. The\u0000rhein may be the effective substance. It may serve as a reference in the quality control of wine processed\u0000rhubarb products.","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36290577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Advances in the research of inhibitors of enzymes of bacterial peptidoglycan biosynthesis]. [细菌肽聚糖生物合成酶抑制剂的研究进展]。

Yaoxue Xuebao Pub Date : 2017-03-01

Fan Liu, Hao-yi Meng, Zheng-yang Sun, Dan-yang Li, Yuan-yuan Jin, Zhao-yong Yang, Shao-jie Wu, Jing Chen

引用次数: 0

[The protective effects of curcumin loaded mesoporous silica nanoparticles on rat cardiomyocytes]. [姜黄素负载介孔二氧化硅纳米颗粒对大鼠心肌细胞的保护作用]。

Yaoxue Xuebao Pub Date : 2017-03-01

Hui-ni Fu, Li Wu, Lian-wen Duan, Shu-zhi Lü

引用次数: 0

[Identification of water buffalo horn and its adulterants using COI barcode]. [利用COI条形码识别水牛角及其掺假物]。

Yaoxue Xuebao Pub Date : 2017-03-01

Xu-zhao Liu, Li-si Zhou, Jin-xin Liu, Jing Jia, Jing-yuan Song, Lin-chun Shi

{"title":"[Identification of water buffalo horn and its adulterants using COI barcode].","authors":"Xu-zhao Liu, Li-si Zhou, Jin-xin Liu, Jing Jia, Jing-yuan Song, Lin-chun Shi","doi":"","DOIUrl":"","url":null,"abstract":"Bubali cornu (water buffalo horn) has been used as the substitute for Cornu rhinoceri asiatici\u0000(rhino horn) in clinical applications, and is the essential ingredient of Angong Niuhuang Wan. In recent years,\u0000there are a number of adulterants on the commercial herbal medicine markets. An efficient tool is required\u0000for species identification. In this study, 155 Bubali cornu samples have been taken from original animals and\u0000collected from commercial herbal medicine markets. 153 COI sequences have been successfully obtained\u0000from 155 samples through DNA extraction, PCR amplification, bidirectional sequencing and assembly. 93\u0000COI sequences have been added to the DNA barcoding database of traditional Chinese animal medicine after\u0000validation using DNA barcoding GAP and tree-based methods. The species identification of the 62 commercial\u0000Bubali cornu medicines has been accomplished on the DNA barcoding system for identifying herbal medicine\u0000using the updated animal medicine database (www.tcmbarcode.cn). Except two samples failed to obtain COI\u0000sequences, 54.8% of the commercial Bubali cornu medicines were water buffalo horns and 29% were yak horns.\u0000Our results showed that yak horn was the major adulterant of Bubali cornu and the DNA barcoding method may\u0000accurately discriminate Bubali cornu and their adulterants. Therefore, we recommend that supervision on the\u0000herbal medicine markets should be strengthened with this new method to warren the effectiveness of herbal\u0000medicines.","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36290580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Transglutaminase-based antibody-drug conjugation: antibody site-specific mutation and identification]. [基于转谷氨酰胺酶的抗体-药物偶联:抗体位点特异性突变和鉴定]。

Yaoxue Xuebao Pub Date : 2017-03-01

Zhao-xiong Ma, Yao Xu, Hong Zhao, Fu-mou Sun, Xin-rong Zhang, Min Wang, Juan Zhang

引用次数: 0

[Design, synthesis and evaluation of a novel MEK protein inhibitors]. 一种新型MEK蛋白抑制剂的设计、合成和评价

Yaoxue Xuebao Pub Date : 2017-03-01

Bin-bin Song, Zi-kuo Zhang, Qing-feng Zhu, Gu He, Ju-zheng Fan

引用次数: 0

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