Zhao-xiong Ma, Yao Xu, Hong Zhao, Fu-mou Sun, Xin-rong Zhang, Min Wang, Juan Zhang
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引用次数: 0
Abstract
Transglutaminase (TG) posttranslational modification of antibody permits more precisely
conjugating. Based on the amino acid sequence of an anti-CD24 antibody (cG7), this article is aimed to
generate a deglycosylated cG7 mutant (cG7Q). Firstly, we introduced additional glutamines at position 297
(N297Q) by site-directed mutagenesis, and then transfected the recombinant plasmids into CHO-s cells via
electroporation method and screened by Dot blot assay. Subsequently, cG7Q was expressed and purified
through Protein A affinity chromatography, further identified by SDS-PAGE electrophoresis and Western blot.
Its affinity was detected with surface plasmon resonance and flow cytometry assay, and ADCC effect was
determined by lactate dehydrogenase (LDH) release. Eventually, a cG7 mutant, cG7Q was successfully
expressed with sequence-specific conjugation sites for further study.
药学学报Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.20
自引率
0.00%
发文量
0
期刊介绍：
Acta Pharmaceutica Sinica B (APSB) is a bimonthly English peer-reviewed online journal in ScienceDirect, which publishes significant original research articles, communications and high quality reviews of recent advances. APSB encourages submissions from all areas of pharmaceutical sciences, including pharmacology, pharmaceutics, medicinal chemistry, natural products, pharmacognosy, pharmaceutical analysis and pharmacokinetics.
APSB is a part of the series Acta Pharmaceutica Sinica, which was founded in 1953. The journal is co-published by Elsevier B.V., in association with the Institute of MateriaMedica, Chinese Academy of Medical Sciences and Chinese Pharmaceutical Association.
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