药学学报最新文献_第3页

[In vivo study of Chuankezhi metabolism in rat]. 川可治在大鼠体内代谢研究。

Yaoxue Xuebao Pub Date : 2017-03-01

Qian-ru Feng, Jie-jing Yu, Jing-jing Zhan, Yi-juan Xu, Yeuk-lung Chow, Shun-jun Xu, Liu Yang

{"title":"[In vivo study of Chuankezhi metabolism in rat].","authors":"Qian-ru Feng, Jie-jing Yu, Jing-jing Zhan, Yi-juan Xu, Yeuk-lung Chow, Shun-jun Xu, Liu Yang","doi":"","DOIUrl":"","url":null,"abstract":"To study the metabolic products of main compounds of Chuankezhi injection in rat, 12 Sprague\u0000Dawley rats were classed into 2 groups, a blank control group and an intermuscular administration group,\u0000respectively. Rat feces and urine samples were collected from 0−24 h and 24−48 h after administration. All\u0000the samples were ultrasonically treated with methanol and then analyzed using LC-LTQ Orbitrap MSn. By\u0000comparison with the total ion chromatogram of samples from the blank control group, the metabolites in the\u0000samples of drug-treated group were screened. These metabolites were further analyzed by multistage product\u0000ion scanning and comparison of retention time with reference substances. As a result, a total of 12 flavonoid\u0000metabolites were tentatively identified from the rat feces and no metabolite was discovered in the rat urine.\u0000Epimedin C and icariin were detected in the rat blood samples after 30 min of administration, but their\u0000metabolites and other original flavones were not detected. Furthermore, no original flavones and their\u0000metabolites were detected in rat blood samples after 2 and 4 h of administration. The potential metabolism\u0000paths were further characterized and the principal in vivo transformation of flavones from Chuankezhi injection\u0000were deglycosylation, dehydration, methylation, oxidation and isomerization in rats.","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"52 3","pages":"449-55"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36290618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[On-line detection of concentration process of Ganmaoling granules by near infrared spectroscopy combined with automatic control system]. [近红外光谱结合自动控制系统在线检测感冒灵颗粒浓缩过程]。

Yaoxue Xuebao Pub Date : 2017-03-01

Xue-song Liu, Jia-shan Chen, Guo-quan Chen, Ping Shao, Yong-jiang Wu

{"title":"[On-line detection of concentration process of Ganmaoling granules by near infrared spectroscopy combined with automatic control system].","authors":"Xue-song Liu, Jia-shan Chen, Guo-quan Chen, Ping Shao, Yong-jiang Wu","doi":"","DOIUrl":"","url":null,"abstract":"Our research was designed for on-line detection of multi-index in the concentration process of\u0000Ganmaoling granules by integration of near infrared spectroscopy and automatic control system. First, on-line\u0000detection system was set up in the concentration tank for Ganmaoling granules production. Spectra were\u0000scanned and values of chlorogenic acid, linarin, solid content and relative density were measured. Models\u0000of partial least squares regression were built and imported into near infrared workstation. By connecting\u0000the control system, real-time multi-index values were determined automatically in the concentration process.\u0000Results showed that correlation coefficients of chlorogenic acid, linarin, solid content and relative density models\u0000were 0.963, 0.989, 0.993 and 0.918, respectively. Relative standard errors of prediction were 3.71%, 4.28%,\u00004.17% and 0.24%, respectively, indicating a good performance and high accuracy of the models. Real-time data\u0000collection during the whole process was measured by the near infrared detecting system in the control system.\u0000In conclusion, the near infrared detection system is able to perform real-time automatic determination of multiindex\u0000in the concentration process of Ganmaoling granules with significant advantages.","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"52 3","pages":"462-7"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36290509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Construction of the brain-targeting drug carrier through imprinting of nicotinic acetylcholine receptor α7]. [烟碱乙酰胆碱受体α7印迹构建脑靶向药物载体]。

Yaoxue Xuebao Pub Date : 2017-03-01

Xue Wang, Ya-hua Wang, Sha Liu, Chong Li

引用次数: 0

[Investigation of the potentially effective components of Semen Ziziphi Spinosae based on “in vitro to in vivo” translation approach]. [基于“体外到体内”翻译方法的酸枣子潜在有效成分研究]。

Yaoxue Xuebao Pub Date : 2017-02-01

Yan Yan, Qiang Li, Chen-hui Du, Jin-ping Jia, Hong-xia Feng, Xue-mei Qin

{"title":"[Investigation of the potentially effective components of Semen Ziziphi Spinosae based on “in vitro to in vivo” translation approach].","authors":"Yan Yan, Qiang Li, Chen-hui Du, Jin-ping Jia, Hong-xia Feng, Xue-mei Qin","doi":"","DOIUrl":"","url":null,"abstract":"The study aims to investigate the effective components of Semen Ziziphi Spinosae (SZR) in\u0000nourishing the heart and tranquilizing the mind. A method of ultra high liquid chromatography (UHPLC)\u0000coupled with Q Exactive high resolution mass spectrometry (HR-MS) was developed. Based on the UV spectra,\u0000retention time and MS spectra, 25 compounds of SZR extract were identified or tentatively characterized,\u0000including 12 flavonoids, 8 triterpenoids saponins, 2 fatty acid and 3 alakoids. The study illuminated the major\u0000chemical components. Twenty bioactive components were determined in rat urine after oral administration of\u0000SZR extract by “in vitro to in vivo” translation approach, including 16 prototype compounds and 4 metabolites.\u0000Spinosin, swertisin, jujuboside A and B were considered as the effective and active constituents in SZR of\u0000the sedative and hypnotic effects, which emodies characteristics of multiple components. It was beneficial\u0000exploration for searching the effective and active constituents of SZR in nourishing the heart and tranquilizing\u0000the mind.","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"52 2","pages":"283-90"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36289258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Molecular cloning and prokaryotic expression of a type II ribosome inactivating protein from Polyporus umbellatus]. [伞形蓼II型核糖体失活蛋白的分子克隆和原核表达]。

Yaoxue Xuebao Pub Date : 2017-02-01

Meng-meng Liu, Yong-mei Xing, Shun-xin Guo

引用次数: 0

[Advances in studies of ileal apical sodium-dependent bile acid transporter]. [回肠根尖钠依赖性胆汁酸转运体研究进展]。

Yaoxue Xuebao Pub Date : 2017-02-01

Mao-xu Ge, Ju-xian Wang, Rong-guang Shao, Hong-wei He

引用次数: 0

[In vitro metabolism of daphnetin in rat liver S9 fractions]. [大鼠肝脏S9组分中瑞香素的体外代谢研究]。

Yaoxue Xuebao Pub Date : 2017-02-01

Si-cheng Liang, Guang-bo Ge, Yang-liu Xia, Xiao-yi Qi, Ao-xue Wang, Cai-xia Tu, Ling Yang

{"title":"[In vitro metabolism of daphnetin in rat liver S9 fractions].","authors":"Si-cheng Liang, Guang-bo Ge, Yang-liu Xia, Xiao-yi Qi, Ao-xue Wang, Cai-xia Tu, Ling Yang","doi":"","DOIUrl":"","url":null,"abstract":"Daphnetin is quickly eliminated in rats after dosing, but the mechanism remains unclear. This\u0000study was aimed to investigate the in vitro metabolism of daphnetin using rat liver S9 fractions (RLS9). The\u0000metabolites formed in RLS9 were identified and the kinetic parameters for different metabolic pathways\u0000were determined. HPLC-DAD-MS analysis showed that daphnetin was biotransformed to six metabolites,\u0000which were identified as 7 or 8 mono-glucuronide and mono-sulfate, 8-methylate, and 7-suflo-8-methylate.\u0000Methylation and glucuronidation of daphnetin exhibited the Michaelis-Menten kinetic characteristics, whereas\u0000the substrate inhibition kinetic and the two-site kinetic were observed for 8-sulfate and 7-sulfate formations. Of\u0000the 3 conjugation pathways, the intrinsic clearance rate for sulfation was highest, followed by methylation and\u0000glucuronidation. By in vitro-in vivo extrapolation of the kinetic data measured in RLS9, the hepatic clearance\u0000were estimated to be 54.9 mL·min−1·kg−1 which is comparable to the system clearance (58.5 mL·min−1·kg−1)\u0000observed in rats. In conclusions, the liver might be the main site for daphnetin metabolism in rats. Sulfation,\u0000methylation and glucuronidation are important pathways of the hepatic metabolism of daphnetin in rats.","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"52 2","pages":"291-5"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36289255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The interaction between BLF and BSA and impact in interaction of RT-BSA-BLF system]. [BLF与BSA的相互作用及其对RT-BSA-BLF系统相互作用的影响]。

Yaoxue Xuebao Pub Date : 2017-02-01

Ming Guo, Yan Wang, Xing-tao Xu

{"title":"[The interaction between BLF and BSA and impact in interaction of RT-BSA-BLF system].","authors":"Ming Guo, Yan Wang, Xing-tao Xu","doi":"","DOIUrl":"","url":null,"abstract":"The binding of rhaponticin to bovine serum albumin (BSA)-bovine lactoferrin (BLF) and the\u0000factors that affect BSA-BLF interaction have been studied by fluorescence spectroscopy and Fourier transform\u0000infrared spectroscopy. In the fluorescence experiment, RT quenched the fluorescence intensity of mixed\u0000proteome and the maximum emission wavelength of BSA, BLF and BSA-BLF proteins system. RT caused\u0000obvious red-shift fluorescence for an interaction between RT and proteome. The interaction between RT and\u0000proteome was impacted by single-component protein molecular interactions and the interaction between RT-BSA\u0000and RT-BLF, the microenvironment of solutions were the factors impacting the interactions between RT and\u0000proteome, which impacted quantitative expression of the general environment micro environmental factors.\u0000In the Fourier transform infrared spectroscopy, the secondary conformation of protein molecules of single\u0000component in the protein group were changed, and the difference of the molecules ’ structure was responsible for\u0000the differences in the molecular conformation changes. The molecules ’ interaction in the single-component\u0000protein affected secondary conformation of the proteins’ molecule. The proteins’ concentration ratio and the\u0000interaction were different in degree of molecular conformational change. These data demonstrates an example\u0000of combination of fluorescence spectrum experiment with Fourier transform infrared spectroscopy in the study of\u0000protein structura.","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"52 2","pages":"271-8"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36289853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Inhibition of transformation from puerarin monohydrate to puerarin dihydrate by polyvinylpyrrolidones during dissolution]. 聚乙烯吡咯烷酮对溶解过程中葛根素一水向二水转化的抑制作用。

Yaoxue Xuebao Pub Date : 2017-02-01

Zhao-yi Zhong, Min Wu, Shuai Qian, Jian-jun Zhang, Yuan Gao

{"title":"[Inhibition of transformation from puerarin monohydrate to puerarin dihydrate by polyvinylpyrrolidones during dissolution].","authors":"Zhao-yi Zhong, Min Wu, Shuai Qian, Jian-jun Zhang, Yuan Gao","doi":"","DOIUrl":"","url":null,"abstract":"Puerarin (PUE), an isoflavone with anti-inflammation, anti-oxidation and neuroprotection effects,\u0000has been widely applied to the treatment of cardiovascular diseases in clinics in China. In the current study, we\u0000reported that the active pharmaceutical ingredient (API) of marketed products was the PUE monohydrate\u0000(PUEMH). During its supersaturated dissolution, the PUE concentration quickly reached a plateau, followed by\u0000a gradually concentration decrease to another lower plateau. In order to explore the internal mechanism of above\u0000phenomenon, the solid residues after saturated dissolution test were characterized by powder X-ray diffraction\u0000(PXRD), thermal gravity analysis (TGA) and Karl Fisher titration (KFT). PXRD suggested that a novel PUE\u0000crystal different from PUEMH formed during its dissolution, the following TGA and KFT confirmed the generation\u0000of PUE dihydrate (PUEDH) with much lower solubility. Moreover, polyvinylpyrrolidones (PVPK12, PVPK30\u0000and PVPK90) were added in the dissolution medium to investigate their potential inhibition effects on such\u0000crystal transformation during dissolution process. We observed that polymers could inhibit the transformation\u0000from PUEMH to PUEDH and result in much higher PUE concentration level than that in pure water.","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"52 2","pages":"302-8"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36288685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The molecular identification of licorice species and the quality evaluation of licorice slices]. 甘草种类分子鉴定及甘草切片质量评价。

Yaoxue Xuebao Pub Date : 2017-02-01

Rui Yang, Wen-dong Li, Yong-sheng Ma, Shan Zhou, Yu-tao Xue, Rui-chao Lin, Ying Liu

{"title":"[The molecular identification of licorice species and the quality evaluation of licorice slices].","authors":"Rui Yang, Wen-dong Li, Yong-sheng Ma, Shan Zhou, Yu-tao Xue, Rui-chao Lin, Ying Liu","doi":"","DOIUrl":"","url":null,"abstract":"Licorice is one of the most common herbs in traditional Chinese medicine, and classified as top\u0000grade in Shen Nong Ben Cao Jing. There are three different original plants of licorice stipulated in Chinese\u0000Pharmacopeia, Glycyrrhiza uralensis Fisch., Glycyrrhiza glabra L., and Glycyrrhiza inflata Bat. However,\u0000previous investigation showed that the pharmacodynamic effects of the three licorices were quite different. It is\u0000very difficult to identify them by the classical identification methods. In order to establish a fast and effective\u0000identification method, we collected 240 licorice plants from 21 populations of 7 provinces, and amplified their\u0000ITS and psbA-trnH sequences. ITS sequences with a full length of 616 bp and psbA-trnH sequences with a full\u0000length of 389 bp were obtained separately. Using DNAMAN to analyze these sequences, 4 variable sites were\u0000found in ITS sequences and 2 ITS haplotypes were determined, and 3 variable sites were found in psbA-trnH\u0000sequences and 4 psbA-trnH haplotypes were determined. With the combination analysis of ITS and psbA-trnH\u0000sequences, the molecular identification method of original licorice was established. Using this method, 40\u0000samples of licorice slices collected from 4 main herbal material markets in China were identified successfully.\u0000Furthermore, the contents of 2 triterpenes, 18α-glycyrrhizic acid and 18β-glycyrrhizic acid, and 4 flavonoids,\u0000liquiritin, isoliquiritin, liquiritigenin, and isoliquiritigenin in these licorice pieces were examined by HPLC and\u0000the results were analyzed using SPSS 21.0. This study provides a new method in identification of licorice,\u0000which may serve as a guideline for quality control of licorice slices.","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"52 2","pages":"318-26"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36289264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0