药学学报最新文献

{"title":"[Interaction between endophytes and host plant and the role of endophytes in genuineness analysis of medicinal plant].","authors":"Jin-long Cui,&nbsp;Shun-xing Guo,&nbsp;Pei-gen Xiao","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Research of plant development and metabolism has drawn lots of attention with the fast development\u0000of science of mycorrhizal biology, molecular biology and metabonomics technology. It has become one of\u0000hot fields in the study of endophytes and plant, which would affect plant ’s metabolite composition. This would\u0000provide opportunity for appraising and modifying traits to medicinal plant, and would also perfect the tranditional\u0000standpoint on forming reason of medicinal plant genuineness. Here we provide a review of theory and\u0000mechanism, research and application of interaction between plant and endophyte. This review may enhance\u0000understanding of medicinal plant, and evaluating the quality of herbs in production.</p>","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"52 2","pages":"214-21"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36287853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Design, synthesis and structure-activity relationship studies of human dihydroorotate dehydrogenase inhibitors].","authors":"Ying-hui Gong,&nbsp;Li Liu,&nbsp;Tian-tian Qi,&nbsp;Hong-lin Li,&nbsp;Li-li Zhu,&nbsp;Zheng-jiang Zhao","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this study, 1-(3-(4-chlorophenyl)-5-methylthio-1H-1,2,4-triazol-1-yl)-butan-1-one discovered\u0000previously in our lab was selected as a inhibitor of human dihydroorotate dehydrogenase ( HsDHODH) for\u0000structural optimization. The co-crystal of HsDHODH with the hit was obtained and analyzed for guiding\u0000the subsequent structural optimization. As a result, a series of novel triazole derivatives were designed and\u0000synthesized as potent HsDHODH inhibitors. Among them, compound (3-(4-chlorophenyl)-5-ethylthio-1H-\u00001,2,4-triazol-1-yl)-furan-2-yl-methanone displayed high potency in the inhibition of HsDHODH with an IC50\u0000value of 1.50 μmol·L−1. Meanwhile, the structure-activity relationships were analyzed based on the biological\u0000data and the co-crystal structure. These results provide a valuable reference for optimization of 1H-1,2,4-\u0000triazole derivatives as HsDHODH inhibitors in the future.</p>","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"52 2","pages":"264-70"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36289860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Antidepressant activity of flavonoid ethanol extract of Abelmoschus manihot corolla with BDNF up-regulation in the hippocampus].","authors":"Hong-die Cai,&nbsp;Wei-wei Tao,&nbsp;Shu-lan Su,&nbsp;Sheng Guo,&nbsp;Yue Zhu,&nbsp;Jian-ming Guo,&nbsp;Da-wei Qian,&nbsp;Xu-dong Cong,&nbsp;Ren-mao Tang,&nbsp;Jin-ao Duan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Abelmoschus manihot (L.) Medic., a folk herbal medicine in China, is a flowering plant belonging\u0000to Abelmoschus L. genus and Malvaceae family, which has been reported with an antidepressant activity. The\u0000study was designed to isolate flavonoids from Abelmoschus manihot corolla and explore the action mechanism\u0000of antidepressant activities. The flavonoids were isolated and purified by D101 macroporous resin column,\u0000polyamide column and Sephadex LH-20 sequentially and identified as myricetin-3-O-β-D-glucoside (1),\u0000gossypetin-8-O-β-D-glucuronide (2, G-8-G), gossypetin-3'-O-β-D-glucoside (3), quercetin-3'-glucoside (4, Q-3-G),\u0000isoquercitrin (5, IQT), hyperoside (6, HY), myricetin (7), quercetin (8, QT). Compounds 2, 4, 5, 6 and 8\u0000(15, 30 and 60 mg·kg−1) were orally administered to mice and the reaction was observed in tail suspension\u0000test (TST) and forced swimming test (FST). Western blot analysis was used in determination of the protein\u0000expressions of brain-derived neurotrophic factor (BDNF), tyrosine receptor kinase B (TrkB) and phosphorylation\u0000eukaryotic elongation factor 2 (p-eEF2). The results revealed that only Q-3-G and G-8-G (15, 30, 60 mg ·kg−1)\u0000significantly reduced the immobility time in FST and TST. Furthermore, Q-3-G and G-8-G remarkably increased\u0000the expression of BDNF and TrkB, and decreased the expression of p-eEF2. These results suggest that\u0000Q-3-G and G-8-G had an obvious antidepressant activity via up-regulation of BDNF expression. The\u0000new observation will provide a new direction in the development of antidepressant in the treatment of major depressive\u0000disorder (MDD).</p>","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"52 2","pages":"222-8"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36287854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Comparative study of the corticosterone and glutamate induced PC12 cells depression model by 1H NMR metabolomics].","authors":"Xiao-yan He,&nbsp;Jian-li Chen,&nbsp;Huan Xiang,&nbsp;Yao Gao,&nbsp;Jun-sheng Tian,&nbsp;Xue-mei Qin,&nbsp;Guan-hua Du","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This study was designed to analyze the change of metabolites in the PC12 cells and its medium\u0000induced by corticosterone (CORT) and glutamate (Glu) by proton nuclear magnetic resonance ( 1H NMR)\u0000metabolomics. The multivariate statistical analysis was employed to identify the difference between control\u0000groups and induced groups, respectively. In addition, metabolite pathway analysis was performed to explore\u0000the characteristic of CORT-induced and or Glu-induced PC12 cells depression model, and to provide the\u0000references for the selection of in vitro depression models as well as the further understanding of the mechanism\u0000on depressive disorders. We found 36 differential metabolites in CORT-induced PC12 cells and medium and\u000042 in Glu-induced PC12 cells. Furthermore, correlation analysis results show that serine and 2-oxoisoleucine\u0000were associated with most differential metabolites in CORT-induced PC12 cells. Lactate and glutathione\u0000were significantly correlated to the vast majority of differential metabolites in Glu-induced PC12 cells. We\u0000speculated that CORT-induced PC12 cell models may affect the fatty acid metabolism and cell membrane\u0000structure, and Glu-induced PC12 cell models may have a difference in the glycolysis and antioxidants.</p>","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"52 2","pages":"245-52"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36287859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[GGI as a gene carrier delivering MDR1 siRNA to A549/DDP cells for reversal of multidrug resistance].","authors":"Zi-rui Wang,&nbsp;Feng Bai,&nbsp;Xiao-ying Zhang,&nbsp;Jia-min Wu,&nbsp;Ling Guo,&nbsp;Zhi Li,&nbsp;Min Feng","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This study was designed to reverse multidrug resistance of lung cancer cells by downregulating MDR1 genes through RNA interference (RNAi) technology. A novel biodegradable cationic polymer (PEG- b- PLG-g-PEIs, GGI) was synthesized and characterized by 1H NMR. The particle size and zeta potential were measured by dynamic light scattering (DLS). The cell viability profile of GGI was tested by MTT method with both A549 and A549/DDP cell lines. Flow cytometry (FCM) technology was used to investigate the efficiency and intensity of delivering siRNA to cells by GGI polymer. RT-PCR and Western blot were used to detect the mRNA and P-gp expression after GGI/MDR1 siRNA transfection assay. The sensitivity of cisplatin administration after transfecting GGI/MDR1 siRNA polyplexs was performed with MTT and Annexin V-FITC/PI methods. The results suggest that the particle size and zeta potential of GGI/siRNA were 150 −200 nm and 16−28 mV. GGI exhibited a lower cell cytotoxity than PEI 25K and higher efficiency of delivering siRNA, which dramatically decreased the expression of MDR1 mRNA and P-gp of A549/DDP cells and increased much sensitivity to cisplatin in A549/DDP cells. GGI holds a great potential in gene delivery as a novel cationic polymer for further investigation.</p>","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"52 2","pages":"309-17"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36289257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Recent progress of reactive oxygen species-responsive drug delivery systems].","authors":"Guang-zhao Lu,&nbsp;Cheng Hou,&nbsp;Yan-qiang Zhong,&nbsp;Ying Lu,&nbsp;Hao Zou","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"52 2","pages":"206-13"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36287855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A new cassane diterpene from Caesalpinia bonduc].","authors":"Wen-chao Tu,&nbsp;Lin-fen Ding,&nbsp;Hui Yang,&nbsp;Liu-dong Song,&nbsp;Xing-de Wu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Five cassane diterpenes were isolated from the 95% ethanol extract of the seeds of Caesalpinia\u0000bonduc (Leguminosea) by a combination of various chromatographic methods, including silica gel, Sephadex\u0000LH-20, and semi-preparative HPLC. On the basis of spectroscopic techniques, their structures were identified\u0000as 3β-acetoxy-cassa-12,14(17),15-trien-7β-ol (1), caesalmin C (2), caesall E (3), caesalpinin MJ (4), and\u00001-deacetylcaesalmin C (5). Among them, compound 1 is a new compound and 2, 4, 5 were isolated from the\u0000plant for the first time.</p>","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"52 2","pages":"279-82"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36289859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Applications of immobilization techniques in the screening of active constituents from traditional Chinese medicines].","authors":"Yu-xiu Yang,&nbsp;Su-ying Li,&nbsp;Qian Zhang,&nbsp;Zhi-ning Xia,&nbsp;Feng-qing Yang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It has been an active approach to screen the active ingredients in traditional Chinese medicines\u0000(TCMs) according to the affinity property between small molecule compounds and biomaterials such as cells,\u0000bacteria and proteins. On the other hand, the biomaterials can be immobilized on a solid support before the\u0000screening procedure. The immobilization method not only can maintain the biological activities of biomaterials,\u0000but also have other advantages such as high efficiency, simple operation, easy to be continuous and automatic, etc.\u0000Carrier materials (solid supports) for the immobilization including silica gel, magnetic materials, hollow fiber,\u0000and the surface plasma resonance sensor chips have been used to immobilize biomaterials and successfully\u0000applied in the screening of active ingredients from TCMs. In this paper, applications of immobilization\u0000techniques in the screening of active components from TCMs were reviewed to provide a scientific reference\u0000to the future applications.</p>","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"52 2","pages":"198-205"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36287851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The progress of novel drug delivery systems].","authors":"Hui-le Gao,&nbsp;Xin-guo Jiang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The development of pharmaceuticals has been providing many kinds of novel drug delivery\u0000systems, which are important for improving therapeutic effect and one of the most important fields in pharmaceutics.\u0000According to their application, we can generally divide the novel drug delivery systems into three categories:\u0000quickly performed drug delivery system, long-term drug delivery system and high effective drug delivery system.\u0000Some diseases, such as asthma, angina pectoris and migraine, require therapeutics urgently, and the drugs have to\u0000be absorbed in several minutes. Therefore, quickly performed drug delivery systems are developed, such as oral\u0000disintegrating tablets and nasal spray. For normal tablets and capsules, especially the drugs with short blood\u0000half life, the drug concentration in blood shows obvious peak-valley phenomenon, which reduces the therapeutic\u0000effect and requires multiple administration. To solve this problem, sustained drug release system was developed,\u0000which could release the drugs slowly and sustainably even in zero-order kinetics. The pulse drug delivery system\u0000was developed that can delayed and pulsed release drug for one or several times. This system is especially\u0000useful in the management of asthma and heart disease, which are often found in midnight or early morning when\u0000patients are in bed. Transdermal drug delivery system could release drugs sustainably and deliver the drugs\u0000through skin to blood circulation, providing long term activity. The water-insoluble drugs are difficult for\u0000pharmaceutical development, thus many methods were developed to improve the solubility and bioavailability of\u0000drugs. Although biopharmaceuticals are important for disease treatment, the application shadows by the poor\u0000stability and low bioavailability. Thus the biopharmaceutical delivery system was developed, which mainly\u0000focused on structure modification and encapsulation by carriers. Considering therapeutic effect requires\u0000interaction between drugs and their targets, it is important to deliver drugs to their targets. Therefore, targeting\u0000delivery systems were developed, which mainly based on the nanoparticles. Furthermore, on-demand release\u0000drug delivery systems are also developed with the property of environment-triggered drug release. In conclusion,\u0000the novel drug delivery systems were reviewed in this study.</p>","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"52 2","pages":"181-8"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36287852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of metoprolol or/and pravastatin on the pharmacokinetics of metformin in rats].","authors":"Yan-rong Ma,&nbsp;Yan-fang Wu,&nbsp;Ying-qin Duan,&nbsp;Guo-qiang Zhang,&nbsp;Xin-an Wu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This study investigates the effects of metoprolol (METO) or/and pravastatin (PRAV) on the\u0000pharmacokinetics of metformin (METF) in rats. Twenty-eight male SD rats were divided into METF group,\u0000METF+METO group, METF+PRAV group and METF+METO+PRAV group. Blood samples were collected\u0000at 10, 20, 40, 60, 90, 120, 180, 240, 360, 480 and 600 min after oral administration of metformin, and\u0000concentration of metformin in plasma was determined by HPLC. Compared to the METF group, Cmax of\u0000metformin was significantly decreased (P < 0.01) and MRT0−t , t1/2 and V were significantly increased in\u0000the METF+METO group; t1/2 was significantly decreased in the METF+PRAV group; Cmax was significantly\u0000decreased and MRT0−t was significantly increased in the METF+METO+PRAV group. Compared to the\u0000METF+METO group, MRT0−t of metformin was significantly decreased in the METF+METO+PRAV group.\u0000Compared to the METF+PRAV group, Cmax of metformin was significantly decreased (P < 0.01), and\u0000MRT0−t , t1/2 and V were significantly increased in the METF+METO+PRAV group. There exist multiple\u0000drug interactions of metformin, metoprolol and pravastatin in rats.</p>","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"52 2","pages":"253-7"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36287856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}