Japanese Journal of Leprosy最新文献_第5页

[Enhanced activation of T lymphocytes by urease-deficient recombinant bacillus Calmette-Guérin producing heat shock protein 70-major membrane protein-II fusion protein]. [产生热休克蛋白70-主膜蛋白- ii融合蛋白的重组calmette - gusamrin重组芽孢杆菌增强T淋巴细胞的激活]。

Japanese Journal of Leprosy Pub Date : 2012-09-01 DOI: 10.5025/hansen.81.199

Masahiko Makino, Tetsu Mukai

{"title":"[Enhanced activation of T lymphocytes by urease-deficient recombinant bacillus Calmette-Guérin producing heat shock protein 70-major membrane protein-II fusion protein].","authors":"Masahiko Makino, Tetsu Mukai","doi":"10.5025/hansen.81.199","DOIUrl":"https://doi.org/10.5025/hansen.81.199","url":null,"abstract":"To activate naïve T cells convincingly using Mycobacterium bovis BCG (BCG), rBCG (BCG-D70M) that was deficient in urease, expressed with gene encoding the fusion of BCG-derived heat shock protein (HSP) 70 and Mycobacterium leprae-derived major membrane protein (MMP)-II, one of the immunodominant Ags of M. leprae, was newly constructed. BCG-D70M was more potent in activation of both CD4+ and CD8+ subsets of naïve T cells than rBCGs including urease-deficient BCG and BCG-70M secreting HSP70-MMP-II fusion protein. BCG-D70M efficiently activated dendritic cells (DC) to induce cytokine production and phenotypic changes, and activated CD4+ T cells even when macrophages were used as APCs. The activation of both subsets of T cells was MHC and CD86 dependent. Pre-treatment of DC with chloroquine inhibited both surface expression of MMP-II on DC and the activation of T cells by BCG-D70M-infected APCs. The naïve CD8+ T cell activation was inhibited by treatment of DC with brefeldin A and lactacystin so that the T cells was activated by TAP- and proteosome-dependent cytosolic cross-priming pathway. From naïve CD8+ T cells, effector T cells producing perforin and memory T cells having migration markers, were produced by BCG-D70M stimulation. BCG-D70M primary infection in C57BL/6 mice produced T cells responsive to in vitro secondary stimulation with MMP-II and HSP70, and more efficiently inhibited the multiplication of subsequently challenged M. leprae than vector control BCG. These results indicate that the triple combination of HSP70, MMP-II and urease depletion may provide useful tool for inducing better activation of naïve T cells.","PeriodicalId":35918,"journal":{"name":"Japanese Journal of Leprosy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30934050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

[Prof. Masao Ota was interested in Kaisyun Hospital of Kumamoto]. [太田正雄教授对熊本开云医院很感兴趣]。

Japanese Journal of Leprosy Pub Date : 2012-09-01 DOI: 10.5025/hansen.81.209

Tomomichi Ono

引用次数: 0

Genotyping of Mycobacterium leprae in Myanmar and possible transmission modes. 缅甸麻风分枝杆菌基因分型及可能的传播方式。

Japanese Journal of Leprosy Pub Date : 2012-09-01 DOI: 10.5025/hansen.81.191

Khin Saw Aye, Yin Thet Nu Oo, Kyaw Kyaw, Aye Aye Win, Masanori Matsuoka

引用次数: 1

[Buruli ulcer and the National Health Insurance Scheme in Ghana]. [布鲁里溃疡与加纳国家健康保险计划]。

Japanese Journal of Leprosy Pub Date : 2012-09-01 DOI: 10.5025/hansen.81.185

Tomoki Niiyama

{"title":"[Buruli ulcer and the National Health Insurance Scheme in Ghana].","authors":"Tomoki Niiyama","doi":"10.5025/hansen.81.185","DOIUrl":"https://doi.org/10.5025/hansen.81.185","url":null,"abstract":"The objectives of this paper are to grasp the current status of an endemic disease in the Republic of Ghana known as Buruli ulcer(BU) and to clarify relationships between the National Health Insurance Scheme(NHIS) and the health care system. As for the method of the study, I have adopted field investigations conducted in Ghana in March, 2009 and August, 2011. All the counter-measurements on BU taken either by the very government or international NGOs have been administered and controlled the disease in accordance with the National Buruli ulcer Control Programme(NBUCP) under the guidance of Global Buruli ulcer Initiative which was established in Geneva, Switzerland in 1998 as an advisory committee of the World Health Organization. BU patients can receive treatments free. The government sponsored NBUCP and direct and indirect donations from various NGOs provide the cost of medical treatments. The Ghanian NHIS of 2003 aimed to ease and improve the health situations of the people. Some of serious endemic diseases like BU, however, are excluded from the schemes. While the NHIS remains ineffective to the diseases like BU, the burden of treatment costs puts the strain on NBUCP. The field researches indicate that the budgets provided by the NBUCP often faile to cover the fundamental medical supplies like bandages. This causes to give an extra burden on the already constrained hospital budgets. Only reliefs the hospitals can rely on are the international aids which often determine the fate of the national disease control. The research reveals that the region's health system remains unsound. Ghana represents such realities of West Africa as a whole.","PeriodicalId":35918,"journal":{"name":"Japanese Journal of Leprosy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5025/hansen.81.185","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30934048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

[Phagocytosis of Mycobacterium leprae down-regulates anti-microbial activity of murine macrophages against Mycobacterium intracellulare]. [麻风分枝杆菌的吞噬作用下调小鼠巨噬细胞对胞内分枝杆菌的抑菌活性]。

Japanese Journal of Leprosy Pub Date : 2012-09-01 DOI: 10.5025/hansen.81.175

Yutaka Tatano, Chiaki Sano, Masako Emori, Hajime Saito, Katsumasa Sato, Toshiaki Shimizu, Haruaki Tomioka

{"title":"[Phagocytosis of Mycobacterium leprae down-regulates anti-microbial activity of murine macrophages against Mycobacterium intracellulare].","authors":"Yutaka Tatano, Chiaki Sano, Masako Emori, Hajime Saito, Katsumasa Sato, Toshiaki Shimizu, Haruaki Tomioka","doi":"10.5025/hansen.81.175","DOIUrl":"https://doi.org/10.5025/hansen.81.175","url":null,"abstract":"Patients with highly bacillated lepromatous leprosy (LL) essentially lack T cell-mediated immune responses specific to Mycobacterium leprae (ML) antigens, resulting in severely impaired host resistance to leprosy bacilli. Such type of immune unresponsiveness characteristic of LL patients is mainly attributable to markedly depressed T cell ability to activate/expand in response to ML antigens. In this study, we examined profiles of antimycobacterial activity of macrophages, which phagocytized leprosy bacilli, because there is another possibility that, in LL patients, host macrophages in the leprosy lesions are impaired in their antimicrobial activity due to their interaction with infected leprosy bacilli, particularly cellular events through binding with and/or internalization of the pathogens, thereby causing the reduction in host resistance to ML pathogens. The present study indicated the following. First, the anti-M. avium complex activity of murine peritoneal macrophages was significantly reduced when they had phagocytosed heat-killed leprosy bacilli. Second, infection of macrophages with leprosy bacilli did not affect macrophage-mediated suppressor activity against T cell proliferative response to Concanavalin A. These findings indicate that macrophage's intracellular signaling pathways that are up-regulated in response to phagocytosis of leprosy bacilli are linked to the signaling cascades participating in macrophage antimicrobial functions, but not cross-talk with those allowing the expression of macrophage's suppressor activity against T cell functions.","PeriodicalId":35918,"journal":{"name":"Japanese Journal of Leprosy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5025/hansen.81.175","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30934047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Collaboration between Korea and Japan for basic research on leprosy]. “韩日麻风病基础研究合作”。

Japanese Journal of Leprosy Pub Date : 2012-09-01 DOI: 10.5025/hansen.81.205

Masanori Matsuoka

引用次数: 0

[Present leprosy situation in the world in 2011]. [2011年世界麻风病现状]。

Japanese Journal of Leprosy Pub Date : 2012-04-01 DOI: 10.5025/hansen.81.145

Shuichi Mori, Sumana Barua, Koichi Suzuki, Norihisa Ishii, Rie Roselyne Yotsu

引用次数: 1

[Pathology specimens suggest a history of Hansen's disease]. [病理标本提示有汉森氏病病史]。

Japanese Journal of Leprosy Pub Date : 2012-04-01

Hiroshi Tsutsumi