V D Mishalov, M Y Goncharuk-Khomyn, V V Voichenko, H Brkic, S B Kostenko, V V Vyun, P P Brekhlichuk
{"title":"Forensic dental identification in complicated fractured skull conditions: case report with adapted algorithm for image comparison.","authors":"V D Mishalov, M Y Goncharuk-Khomyn, V V Voichenko, H Brkic, S B Kostenko, V V Vyun, P P Brekhlichuk","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the perspective of using an adapted algorithm for digital images comparison while providing forensic dental identification in complicated fractured skull conditions by ante-mortem and post-mortem radiographical data sets.</p><p><strong>Materials and methods: </strong>Ante-mortem orthopantomogram and post-mortem peri-apical X-ray images were converted in *.jpeg format with their further import into GIMP 2.10 software (The GIMP Development Team). Segmentation of OPG-image was provided in topographical projections of jaw segments obtained directly from the victim. Comparison of analyzed image segments was provided manually within GIMP 2.10 software using functions of \"Layers\" and \"Opacity\" through the proposed algorithm.</p><p><strong>Results: </strong>Considering the fact that 20 positive concordant dental identifiers overall were verified during comparison of AM and PM X-ray datasets, we can conclude that odontological identity was established. All above-mentioned discrepancies could be classified as explainable. Inter-agreement rate between two investigators considering correspondence between AM and PM datasets reached Cohen's kappa level which is equal to 0,97, while positive 100% agreement was reached considering 21 out of 24 analyzed characteristics.</p><p><strong>Conclusion: </strong>Available AM and PM radiographical datasets represent a sufficient information for effective forensic dental identification, even if such were obtained by different roentgenological techniques (orthopantomography and periapical radiography). Using of an adapted algorithm for digital images comparison with forensic dental purposes could potentially overcome cognitive bias and observer's effect, speed up the process of analysis and increase the accuracy and inter-agreement rate while referencing AM and PM datasets.</p>","PeriodicalId":35728,"journal":{"name":"Journal of Forensic Odonto-Stomatology","volume":"39 2","pages":"45-57"},"PeriodicalIF":0.0,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8630450/pdf/JFOS-39-2-45.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9431381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S Manthapuri, S R Bheemanapalli, L P Namburu, S Kunchala, D Vankdoth, S B Balla, V L Bathala, A L Kasabu
{"title":"Can root pulp visibility in mandibular first molars be used as an alternative age marker at the 16 year threshold in the absence of mandibular third molars: an orthopantomographic study in a South Indian sample.","authors":"S Manthapuri, S R Bheemanapalli, L P Namburu, S Kunchala, D Vankdoth, S B Balla, V L Bathala, A L Kasabu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In many countries, the 16 years of age threshold is considered to be legally relevant according to the law. This research aims to ascertain the sensitivity and specificity of Olze et al. stages of root pulp visibility (RPV) in a sample of 760 south Indian children aged between 12 and 20 years, with an age threshold of 16 years, using receiver operating characteristic curves and area under the curve (AUC). Spearman's rho correlation showed a strong positive correlation between the RPV stages and age. No significant difference between the right and left lower first molars was seen. RPV Stage 2 showed the highest AUC in both females (0.813) and males (0.790). The performance of the RPV Stage 2 to discriminate the legal age threshold of 16 years resulted in the sensitivity, specificity and accuracy values of 0.61, 0.96 and 0.77 in males, 0.65, 0.97 and 0.80 in females. It resulted in 3.6% and 2.9% of false positives and 38.5% and 34.5% of false negatives in both sexes. Even though, RPV Stage 2 can discriminate reasonably well between two age categories, due to the high percentage of false negatives we recommend its use in conjunction with other age estimation methods.</p>","PeriodicalId":35728,"journal":{"name":"Journal of Forensic Odonto-Stomatology","volume":"39 2","pages":"21-31"},"PeriodicalIF":0.0,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8630453/pdf/JFOS-39-2-21.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9431380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K Saranya, S R Ponnada, J J Cheruvathoor, S Jacob, G Kandukuri, M Mudigonda, A L Kasabu, S B Balla
{"title":"Assessing the probability of having attained 16 years of age in juveniles using third molar development in a sample of South Indian population.","authors":"K Saranya, S R Ponnada, J J Cheruvathoor, S Jacob, G Kandukuri, M Mudigonda, A L Kasabu, S B Balla","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Juvenile crime or delinquency has been increasing at an alarming rate in recent times. In many countries, including India, the minimum age for criminal responsibility is 16 years. The present study aimed to estimate the probability of a south Indian adolescent either being or being older than the legally relevant age of 16 years using Demirjian's tooth formation stages. Orthopantomograms (OPG) of 640 south Indian adolescents (320 boys and 320 girls) aged between 12 and 20 years were retrospectively analyzed. In each OPG, Demirjian's formation stage of the mandibular left third molar was recorded and the data was subjected to statistical analysis. Descriptive and Pearsons correlation statistics were performed. The empirical probabilities were provided relative to the medico-legal question of predicting 16 years of age. The distribution of age throughout the 10th, 25th, 50th, 75th and 90th percentile follows a logical distribution pattern horizontally and vertically. Pearson's correlation statistics showed a strong positive correlation between the Demirjian's stages and age for both sexes. Therefore, it can be concluded that stage \"F\" can be used to predict the attainment of age equal to or older than 16 years with a probability of 93.9% for boys and 96.6% for girls.</p>","PeriodicalId":35728,"journal":{"name":"Journal of Forensic Odonto-Stomatology","volume":"39 1","pages":"16-23"},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8590846/pdf/JFOS-39-1-16.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9480406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Human identification through DNA analysis of teeth using powder-free method - A case study.","authors":"N Kumar, A Sharma","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Change is the universal law of nature, and human bodies after death cannot be an exception for a long time. In forensic science, the tissue from the hardest part of the human body is the only hope to establish the identity, and maternity/paternity of unidentified dead bodies. In this case, a foreign national on a tourist visa to one of the Himalayan states went missing when passing through a dense forest. His relatives could not trace him despite the best efforts of the search team, because of inaccessible hilly terrain. Later on, shepherds while grazing their livestock in the forest area accidentally came across the fragmented remains of a human skeleton. They informed the villagers, and then the police. Teeth collected during the autopsy and blood samples of the putative son, and wife of the missing foreign national on FTA (Flinders Technology Associates) cards were sent to DNA Division, State Forensic Science Laboratory, Junga, Shimla, Himachal Pradesh to establish the identity. DNA profiles obtained from the blood samples of the putative son, wife of missing foreign national, and teeth showed a complete, and concordant match, which established the identity of the skeleton. Moreover, the probability of paternity (>99.99%) between unidentified deceased person and the putative son also assessed the identity of the deceased. Hence, human teeth from unidentified dead bodies can establish the identity of unidentified deceased persons.</p>","PeriodicalId":35728,"journal":{"name":"Journal of Forensic Odonto-Stomatology","volume":"39 1","pages":"45-52"},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8590847/pdf/JFOS-39-1-45.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9480410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A L Rezende Machado, B S Borges, R Cameriere, C E Palhares Machado, R E Alves da Silva
{"title":"Evaluation of Cameriere and Willems age estimation methods in panoramic radiographs of Brazilian children.","authors":"A L Rezende Machado, B S Borges, R Cameriere, C E Palhares Machado, R E Alves da Silva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The importance of age estimation in the forensic field is inherent to the process of establishing the biological profile of children, sub-adults and adults. The established profile might be useful for the identification of deceased victims or living individuals when it comes to age of legal interest. In parallel, age estimation is also investigated for clinical purposes, especially for the diagnosis of dental and bone maturation. Several studies were developed to provide accurate age estimation methods based on skeletal and dental development. This study aimed to apply and compare Cameriere's and Willems' methods for dental age estimation in a Brazilian sample. Two examiners performed image analysis and method application in 180 panoramic radiographs of Brazilian children aged 6-14 years old. The ages estimated with both methods revealed a good correlation with the chronological ages of Brazilian boys and girls. Cameriere's method showed a slight underestimation of 0.05 years for girls and 0.03 for boys. Willems' method, on the other hand, showed an overestimation of -0.47 years for girls and -0.39 for boys. Better age estimates were obtained combining the outcomes of both methods. In practice, Cameriere's and Willems' methods reached reliable outcomes and could be applied for dental age estimation purposes.</p>","PeriodicalId":35728,"journal":{"name":"Journal of Forensic Odonto-Stomatology","volume":"38 3","pages":"8-15"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565658/pdf/JFOS-38-3-08.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9794252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative forensic analysis of reverse root canal filing and conventional method for DNA isolation from extracted teeth under different environmental conditions: A prospective study.","authors":"A A Khan, A B Urs, J Augustine, H Singh","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The grinding of a whole tooth specimen has been considered the conventional method to extract genomic deoxyribonucleic acid (DNA) in forensic science. However, we have tried the less destructive reverse root canal filing (RRCF) method without disturbing the morphology of the tooth to achieve competent amplifiable DNA. A total of 27 pairs of bilateral intact extracted teeth from the same subject were used in three different simulated environmental conditions for the respective RRCF and conventional methods: (a) soil burial for six months, (b) incineration at 200º C for four minutes, and (c) immersion in water for two months. Qualitative agarose gel electrophoresis assessment and downstream amplification were performed. The results showed significantly higher mean DNA concentration for the RRCF method in all three environmental conditions (p value = 0.008) in comparison to the conventional method. However, comparable qualitative results were found in both methods for the mean DNA concentration for incinerated (159.49 ng/ml), soil (119.52 ng/ml), and water (108.60 ng/ml) samples. It was concluded that the RRCF method is better quantitively (ng/ml) and comparable in terms of quality with respect to the conventional method, with the added advantage of preservation of the tooth morphology.</p>","PeriodicalId":35728,"journal":{"name":"Journal of Forensic Odonto-Stomatology","volume":"38 3","pages":"35-41"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565656/pdf/JFOS-38-3-35.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9794254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R Cameriere, L A Velandia Palacio, M Marchetti, F Baralla, M Cingolani, L Ferrante
{"title":"Child brides: the age estimation problem in young girls.","authors":"R Cameriere, L A Velandia Palacio, M Marchetti, F Baralla, M Cingolani, L Ferrante","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aim of this work is to study a sample of girls from 15 different countries using Third Molar Maturity Index (I3M ), to assess the probability that a girl has reached the legal age of 18 years. The studied sample consisted of 3228 Orthopantomograms of healthy female subjects from 15 different countries. The cut-off value of I3M = 0.08 was tested to discriminate adults (≥18 years) and minors (<18 years). X-ray images were processed by computer-aided drafting program ImageJ. The information on sensitivity and specificity of I3M coming from the 15 countries was pooled together using a bivariate Bayesian modeling approach. Specificity of the I3M test did not change when the country was considered, and its value remains greater than 85% for each studied country. This method is useful to estimate the age of the girls involved in suspected early marriage because of the high probability of correctly identifying a minor with similar results observed among tested populations.</p>","PeriodicalId":35728,"journal":{"name":"Journal of Forensic Odonto-Stomatology","volume":"38 3","pages":"2-7"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565659/pdf/JFOS-38-3-02.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9794255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Volume analysis of second molar pulp chamber using cone beam computed tomography for age estimation in Egyptian adults.","authors":"M A Helmy, M Osama, M M Elhindawy, B Mowafey","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Aim: </strong>The present work aimed to evaluate age-related variations in the dental pulp chamber volume of second molars using cone beam computed tomography (CBCT) imaging, in order to establish a specific mathematical model for second molars and measure its accuracy, especially in the case of Egyptian adults.</p><p><strong>Subjects and methods: </strong>From 187 subjects between 21-50 years of age, CBCT images of 257 maxillary and 248 mandibular second molars were included. A mathematical model for human age estimation was established. An independent additional set of CBCT images was obtained to test the model's accuracy.</p><p><strong>Results: </strong>For maxillary and mandibular teeth, R2 for the pooled sexes were 0.51 and 0.52, and SEE were 5.92 and 5.71, respectively. A model for each sex was established, due to the significant difference between them, where R2 was equal to 0.668 and 0.650 in males and 0.46 and 0.48 in females, concerning maxillary and mandibular teeth, respectively. When testing the validation samples, the mean absolute error (MAE) between the actual and estimated ages from the pooled sex model were 4.89 and 4.61 for maxillary and mandibular teeth, respectively.</p><p><strong>Conclusion: </strong>The pulp chamber volume of second molars is a relatively accurate indicator for age estimation in Egyptian adults.</p>","PeriodicalId":35728,"journal":{"name":"Journal of Forensic Odonto-Stomatology","volume":"38 3","pages":"25-34"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565657/pdf/JFOS-38-3-25.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9794253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L Gonçalves do Nascimento, R L Ribeiro Tinoco, A P Lacerda Protasio, I L Arrais Ribeiro, B Marques Santiago, R Cameriere
{"title":"Age estimation in north east Brazilians by measurement of open apices.","authors":"L Gonçalves do Nascimento, R L Ribeiro Tinoco, A P Lacerda Protasio, I L Arrais Ribeiro, B Marques Santiago, R Cameriere","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Dental age (DA) estimation is an extensively investigated resource used by forensic science. This study aimed to evaluate the applicability of the Measurement of Open Apices for DA estimation in north east Brazilians. A total of 429 orthopantomographs of individuals aged 5 to 14.99 years were used. The sample was distributed according to the age groups 5-6.99, 7-8.99, 9-10.99, 11-12.99 and 13-14.99 years, and the data were analyzed descriptively and by linear regression (α= 5%). The majority of the radiographs were from females (n = 241; 56.2%), with an overall mean age of 12 years (± 2.12). A significant difference was observed between DA and chronological age (CA) in the total sample and specifically in females and males. The method underestimated CA by 0.31 year (total sample) and by 0.3 and 0.32 year in females and males, respectively. In contrast, the method overestimated CA in the groups 5-6.99 and 7-8.99 years, with a mean difference (MD) of 0.48 year (p = 0.007) and 0.17 year (p = 0.182), respectively. In the other groups, DA was predicted to be below CA, with a significant difference in the group 13-14.99 (0.75 year). Based on the regression analysis, a correction factor was proposed from the original formula for this population, thereby reaching a predictive power of approximately 80%. To conclude, this method is applicable to the study population aged 5 to 13 years as the estimates obtained did not exceed the error limit of ±1 year.</p>","PeriodicalId":35728,"journal":{"name":"Journal of Forensic Odonto-Stomatology","volume":"38 2","pages":"2-11"},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8559902/pdf/JFOS-38-2-02.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9430846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterization of lip prints in a Portuguese twins' population.","authors":"S Braga, M L Pereira, B Sampaio-Maia, I M Caldas","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Lip print patterns are referred to as unique to each individual, but controversy exists surrounding twins. In this study, the lip prints of 19 pairs of monozygotic and 47 pairs of dizygotic twins were studied. The left lower lip was photographed and the furrows were classified using Renaud's classification. Results showed the same lip pattern was found only in one monozygotic pair (5.3%) and in 4 dizygotic pairs (8.5%), and no significant statistical differences were found between groups (p > 0.05). In monozygotic twins only type C furrows presence displayed statistical significant differences (p=0.034). As for dizygotic twins, there were statistical significant differences in the frequency of type A (p=0.005) and type G furrows (p=0.018). As for the most common types, both groups displayed a higher prevalence of vertical furrows (type B: 97.4% and 96.8%, type A: 86.8% and 87.2%, in monozygotic and dizygotic, respectively). The least frequent furrow type was type I and type E in monozygotic (2.6% and 5.3%, respectively) and types E, F and I, in dizygotic (6.4%, 7.4%. and 7.4%, respectively). Our results seem to point out that lip print patterns should be useful carefully in twins' identification.</p>","PeriodicalId":35728,"journal":{"name":"Journal of Forensic Odonto-Stomatology","volume":"38 2","pages":"40-46"},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8559899/pdf/JFOS-38-2-40.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9430850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}