Mobina Parveen, S. Mitra, J. Tah, Narayan Chandra Chattopadh
{"title":"Study of Intraspecies Variation in Seed Coat Micro-Morphology of Amaranthus hybridus by Scanning Electron Microscope","authors":"Mobina Parveen, S. Mitra, J. Tah, Narayan Chandra Chattopadh","doi":"10.3923/IJPBG.2015.198.205","DOIUrl":"https://doi.org/10.3923/IJPBG.2015.198.205","url":null,"abstract":"Seed character is an important part of any crop plant. The seeds of Amaranthus hybridus (Family-Amaranthaceae) are small in size and not possible to differentiate easily with naked eyes. A little work has been focused on seed morphology of Amaranthus hybridus but no specific work on seed micromorphology has yet been documented. Keeping all these views in mind, we have undertaken the venture for observing possible microfeatures under Scanning Electron Microscope (SEM). The micro-morphological typology of seed surfaces was investigated in eight accessions of Amaranthus hybridus using scanning electron microscopy. Morphological studies showed that seeds were either pyriform or sub-pyriform or sub-spherical in shape. Pleurogram was found on the seed surface in some accessions. The ornamentations of exo-testa were found to be either polygonal or reticular or crosslinked type or spindle shaped or scalariform or undulated lump with tertiary depositions or slits. The aim of this present study was to explore the seed characters which are useful as a tool for identifying crop species and variations among those accessions too. The micromorphological characteristics of the seed coats may provide valuable information for identification of seeds.","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117030737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Detection of sweet potato (Ipomoea batatas L.) leaf curl virus (SPLCV) in Ghana using visual symptomatology and PCR technique","authors":"E. Arkorful, F. Addae-Frimpomaah","doi":"10.3923/IJPBG.2015.106.115","DOIUrl":"https://doi.org/10.3923/IJPBG.2015.106.115","url":null,"abstract":"","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"97 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134079733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Variability, Heritability and Genetic Advance for Some Yield and Yield Related Traits and Oil Content in Ethiopian Coriander (Coriandrum sativum L.) Genotypes","authors":"Geremew Awas, F. Mekbib, A. Ayana","doi":"10.3923/IJPBG.2015.116.125","DOIUrl":"https://doi.org/10.3923/IJPBG.2015.116.125","url":null,"abstract":"Coriander is a diploid annual plant, belonging to the Apiaceae/Umbelliferae family and it plays an important role in the Ethiopian domestic spice trade. Eighty one Ethiopian coriander genotypes were evaluated in 9×9 simple lattice designs for genetic variability in seed yield and yield related traits at Adami Tulu Agricultural Research Center during 2011 main cropping season. Data were collected on 21 yield and yield related characters. The analysis of variance showed that genotypes differed significantly for all characters studied with exception of umbel number per plant, number of seeds per plant and number of seeds per umbellete. Highest Genotypic Coefficient of Variation (GCV) was recorded for leaf number per plant followed by plant height at flowering and seed yield per hectare, in contrast lowest GCV were recorded for number of seed per plant, number of seed per umbellete and umbel number per plant. The highest Phenotypic Coefficient of Variation (PCV) was similarly recorded for leaf number per plant followed by, seed yield per hectare, plant height at flowering and harvest index per plant. The highest broad sense heritability values was recorded for days to 50% flowering, days to emergence, days to maturity and days to start of flowering. However, lowest heritability was recorded for umbel number per plant, number of seed per umbellete and number of seed per plant. The highest genetic advance as percent of mean was recorded for leaf number per plant, plant height at flowering and number of secondary branches. While the lowest genetic advance as a percent of mean was recorded for number of seed per plant, number of seed per umbellete and umbel number per plant.","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"12 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114110359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Devaraja Achar, George Danial, M. Awati, M. Udayakumar, T. G. Prasad
{"title":"Study of Genetic Diversity of Coffea canephora Pierre ex A. Froehner Accessions by RAPD Markers","authors":"Devaraja Achar, George Danial, M. Awati, M. Udayakumar, T. G. Prasad","doi":"10.3923/IJPBG.2015.95.105","DOIUrl":"https://doi.org/10.3923/IJPBG.2015.95.105","url":null,"abstract":"Random amplified polymorphic DNA (RAPD) markers were used to assess the level of genetic variability among 35 robusta coffee accessions consisting exotic and private estate collections having good yield performance under drought conditions DR (drought resistance) accessions. Seventeen informative RAPD markers were generated 212 loci, of which 122 loci found polymorphic (58%). The dendrogram was drawn to visualize relationships among the accessions, wherein three major clusters were formed, where in exotic collections showed closely relatedness with local estate collections demonstrated the narrow genetic base but no trends of significant genetic variance. Even in the dissimilarity matrix constructed by Squared Euclidean Distances (SED) and three-dimensional Principal Component Analysis (PCA) also conferred close distribution of the accessions. The correlation between dissimilarity matrices generated by these methods (r = 0.3) indicated they are positively associated but the relationship was non-significant. However, Drought Resistance (DR) accessions grouped distinctly, which can be phenotyped and used as genetic material for breeding for drought resistance.","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"206 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133905057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Assessment of Negative Environmental Impacts of Drainage and Irrigation Canals on Ipomoea carnea Jacq. in Egypt","authors":"R. Rizk, M. Soliman, Z. Baka, A. A. Saoud","doi":"10.3923/IJPBG.2015.143.162","DOIUrl":"https://doi.org/10.3923/IJPBG.2015.143.162","url":null,"abstract":"This investigation demonstrates the effect of different environmental pollutants of drainage and irrigation canals on Ipomoea carnea Jacq. collected from ten different sites belongs to Dakahlia Governorate. This goal is achieved by using varietal tools as heavy metal analysis such as (Co2+, Cd2+, Pb2+, Cu2+ and Zn2+) of water, soil and plant leaves samples collected from each site, mitotic index, phase index, pollen abortion assay, protein profile using SDS-PAGE technique and molecular study using Random Amplified Polymorphic DNA (RAPD) technique. The effects of heavy metals on I. carnea induced a considerable changes in mitotic index and produced number of chromosomal abnormalities such as multinucleated cells at interphase, micronucleus at prophase, stickiness, non-congression, two groups, chromosome ring and disturbed at metaphase, late separation, laggard, bridge and diagonal in anaphase and telophase and reduced the frequency of pollen fertility (low stainability). The damage of DNA was reflected by changes in protein profile and DNA fingerprinting of I. carnea through the variation in bands by the appearance or disappearance of these bands.","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"103 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114706044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Clonal Assessment of Sweet Potato (Ipomoea batatas (L.) Lam.) Lines for Flower and Seed Characteristics in Jos-Plataeu, Nigeria","authors":"Y. P. Mwanja, S. Wuyep, E. E. Goler","doi":"10.3923/IJPBG.2015.136.142","DOIUrl":"https://doi.org/10.3923/IJPBG.2015.136.142","url":null,"abstract":"A study was carried out during the wet season of 2013 at the Potato Research Farm of the National Root Crops Research Institute (NRCRI), Kuru, Plateau State, to assess five sweet potato clones procured from the NRCRI, Umudike, Nigeria, for flowering and seed characteristics. The clones TIS.2532.OP.1.13, TIS.87/0087, CIPM 3, CIPM 31 and Ex-Igbariam were laid out in a randomized complete block design with three replications. The results showed that the mean number of days to onset of flowering was highest in the clone CIPM 31 (102 days after planting) and lowest in the clone TIS.2532.OP.1.13 (56.67 days after planting). The highest mean number of flowers per plant was observed in the clone CIPM 31 and lowest in the clone TIS.2532.OP.1.13. The mean seed weight per plant was highest in clone TIS.2532.OP.1.13 and lowest in the clone CIPM.31. The study showed that the pattern of flowering and the potential of seed production in the sweet potato varied with clone. There is, therefore, high prospect for improving the sweet potato through hybridization and controlled pollination in the Jos-Plateau environment.","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129482027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Tolerance of Fifteen Soybean Germplasm to Low pH Condition","authors":"H. Kuswantoro","doi":"10.3923/IJPBG.2015.189.197","DOIUrl":"https://doi.org/10.3923/IJPBG.2015.189.197","url":null,"abstract":"Acidity is widespread in the world, including Indonesia that most of the dryland faces acidity. On the other hand, soybean, as one of the main crops in Indonesia, is a plant that is more sensitive to low pH than other crops. Hence, it is needed an attempt to develop soybean variety that is tolerant to acidity to be grown in acidic area. The objective of the research was to find out soybean germplasm that tolerance to low pH. Research was carried out in Seed Laboratory of Indonesian Legume and Tuber Crops Research Institute, Malang, Indonesia, with factorial design of randomized complete block design. The first factor was pH level that consisted of two levels, i.e. pH 7 and pH 4. The second factor was soybean germplasm that consisted of fifteen soybean genotypes. Results showed that there were different responses among the fifteen genotypes to pH treatments. Based on the Acid Soil Adaptation Index (ASAI), there were two promising genotypes that can be considered as gene sources for developing soybean variety for low pH tolerance. The first genotype was MLGG 0471 that had the highest ASAI on all of the seven characters and the second genotype was MLGG 0064 that had the highest ASAI on four characters.","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114819867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Variability, Heritability and Genetic Advance for Some Yield and Yield Related Traits in Barley (Hordeum vulgare L.) Landraces in Ethiopia","authors":"F. Addisu, T. Shumet","doi":"10.3923/IJPBG.2015.68.76","DOIUrl":"https://doi.org/10.3923/IJPBG.2015.68.76","url":null,"abstract":"Genetic diversity is essential for genetic improvement of a given crops. If the information on genetic diversity is not enough to utilize, the available genetic diversity study should be crucial. The objective of this study was to estimate the variability, heritability and genetic advance of barley landraces collection from Gamo highlands of Ethiopia. Accordingly, thirty six barley landraces were evaluated at Chencha, Southern Ethiopia in 2014/2015 cropping season to obtain information on genetic variability, heritability and genetic advance for grain yield and 12 yield related characters in Ethiopian barley landraces. The plot design used for the experiment was a randomized complete block design with three replications. The analysis of variance for the 36 barley landraces revealed significant difference among the landraces for the 13 quantitative characters studied, except spike length. The phenotypic coefficients of variability were higher than genotypic coefficients of variability for all of the characters indicating each and every characters were influenced by environmental factors up to some extent. The greater difference between GCV and PCV was observed spike and peduncle length indicating that these characters were influenced by environmental factors to greater extent. Heritability coupled with high genetic advance was observed for characters biomass per plant, grain yield and number of tiller per plant indicating that selection for these characters could be more effective due to additive gene action. Thus, this study revealed the presence of sufficient variability among the barley landraces in the country that can be exploited for germplasm enhancement.","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134399834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of some Chemical Mutagens on the Growth, Phytochemical Composition and Induction of Mutations in Khaya senegalensis","authors":"G. Mostafa","doi":"10.3923/IJPBG.2015.57.67","DOIUrl":"https://doi.org/10.3923/IJPBG.2015.57.67","url":null,"abstract":"Induced mutation using chemical mutagen is a method to create genetic variation resulting in new varieties with better characteristics. However, their effects in forest trees have received relatively little attention, particularly in Khaya senegalensis. Here, I study the effect of sodium azide and dimethyl sulphate on the growth and phytochemical composition of Khaya senegalensis, in addition to produce genetic variation on the vegetative growth. Seeds of Khaya senegalensis were soaked in dimethyl sulphate solutions (0, 1000, 2000, 3000, 4000 and 5000 ppm) and sodium azide solutions (200, 400, 600, 800 and 1000 ppm) for 15 h. Number of branches and leaves increased significantly on plants treated with 2000 ppm dimethyl sulphate in both seasons. The concentration of 3000 ppm dimethyl sulphate (DMS) increased significantly plant height in the first season but did not differ significantly in the second season. Plants treated with all concentrations of sodium azide increased alkaloid contents in the leaves and bark in both seasons. In addition, they enhanced the accumulation of saponins. The treatments of 4000 ppm dimethyl sulphate in the second season produced dwarfed plant with reddish stem. While, the treatment of 3000 ppm dimethyl sulphate produced plant having reddish pedicel leaflets. In addition, plant with biggest and fast growth was found using 300 ppm dimethyl sulphate in the second season, this last mutant was more genetically distinct to control as found by peroxidase isozyme patterns.","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"79 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133016902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of Genetic Diversity in Brassica juncea (L.) Using Protein Profiling and Molecular Marker (RFLP)","authors":"J. I. Mir, S. Islam, R. Kudesia","doi":"10.3923/IJPBG.2015.77.85","DOIUrl":"https://doi.org/10.3923/IJPBG.2015.77.85","url":null,"abstract":"An understanding of the extent, distribution and patterns of genetic variation is useful for estimation of any possible loss of genetic diversity and assessment of genetic variability and its potential use in breeding programs, including establishment of heterotic groups. This study assessed patterns of genetic diversity and relationships among five accessions of Brassica juncea using sodium dodecyl sulphate and restriction fragment length polymorphism marker systems. The protein profile showed low level of polymorphism (28.57%). The RFLP marker showed a high degree of polymorphism (87.5%) among the five accessions of Brassica juncea. A total of 8 clear bands were scored in all the five accessions. Among the 8 scorable bands calculated, only one band at position 2 with 820 kb was monomorphic and the rest were polymorphic. Upon UPGMA analysis the dendrogram clustered the five accessions into two main clusters. Cluster 1 comprising three accessions IC 343120, IC 248997 and IC 426377. Cluster 2 comprising two accessions IC 426384 and IC 312514. The low polymorphism results of SDS-PAGE showed that SDS-PAGE has limited use in Brassica because of the conservative nature of seed proteins. However, RFLP markers are superior to SDS-PAGE because they provide better coverage of the genome and reveal higher level of polymorphism.","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124933114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}