Huei-Mei Chen, Chen-yu Lin, Miho Yoshida, P. Hanson, Roland Schafleitn
{"title":"Multiplex PCR for Detection of Tomato Yellow Leaf Curl Disease and Root-Knot Nematode Resistance Genes in Tomato (Solanum lycopersicum L.)","authors":"Huei-Mei Chen, Chen-yu Lin, Miho Yoshida, P. Hanson, Roland Schafleitn","doi":"10.3923/IJPBG.2015.44.56","DOIUrl":"https://doi.org/10.3923/IJPBG.2015.44.56","url":null,"abstract":"Tomato yellow leaf curl virus and root-knot nematodes cause diseases in tomato that can lead to heavy production losses. Resistance genes against both pathogens are available and used in breeding. Molecular markers for resistance gene alleles greatly enhance selection of resistant plants in breeding. In order to make marker-assisted selection for the most commonly used resistance genes in tomato breeding more effective, we have developed a multiplex Polymerase Chain Reaction (PCR) assay to simultaneously assess the genotype at four resistance loci. For this purpose, we have selected available markers for the tomato yellow leaf curl disease resistance gene loci Ty-2 and Ty-5 and for the root-knot nematode resistance gene Mi-1 and have developed a new marker for the tomato yellow leaf curl disease resistance gene locus Ty-1/3 to be incorporated in a multiplex PCR assay. The assay correctly predicted the genotypes of tomato breeding lines known to be homo-or heterozygous for the resistance or susceptibility alleles at the Ty-1/3, Ty-2, Ty-5 and Mi-1 gene loci. In wild tomato (Solanum chilense) accessions LA1969 and LA1932, the markers failed to identify alleles conditioning susceptibility at the Ty-2 and Ty-5 loci. Replacement of a single marker of the multiplex assay by another marker was possible without affecting the accurateness of the assay, as long as the size differences of the DNA fragments of the markers were sufficiently large. Combining four markers for resistance alleles commonly used in tomato breeding into a multiplex assay offers a significant cost reduction for marker-assisted breeding in tomato.","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"92 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115281167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Diallel Analysis of Some Yellow Maize Inbred Lines under Low and Normal Nitrogen Levels","authors":"M. Kamara","doi":"10.3923/IJPBG.2015.32.43","DOIUrl":"https://doi.org/10.3923/IJPBG.2015.32.43","url":null,"abstract":"","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125509195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Abdel-Moneam, M. Sultan, S. Sadek, M. S. Shalof
{"title":"Combining abilities for yield and yield components in diallel crosses of six new yellow maize inbred lines.","authors":"M. Abdel-Moneam, M. Sultan, S. Sadek, M. S. Shalof","doi":"10.3923/IJPBG.2015.86.94","DOIUrl":"https://doi.org/10.3923/IJPBG.2015.86.94","url":null,"abstract":"","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134430537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correlation and Path Coefficient Studies of Yield and Yield Related Characters in Tannia (Xanthosoma sagittifolium (L.) Schott) Genotypes","authors":"Solomon Fantaw, A. Nebiyu, Tewodros Mulualem","doi":"10.3923/IJPBG.2014.241.254","DOIUrl":"https://doi.org/10.3923/IJPBG.2014.241.254","url":null,"abstract":"","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"208 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122512787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correlation and Path Coefficient Analysis Study among Seed Yield and Oil Content in Ethiopian Coriander (Coriandrum sativum L.) Genotypes","authors":"Geremew Awas, F. Mekbib, A. Ayana","doi":"10.3923/IJPBG.2014.224.240","DOIUrl":"https://doi.org/10.3923/IJPBG.2014.224.240","url":null,"abstract":"","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"125 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115903236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Selection of Barley Varieties for their Yield Potential at Low Rain Fall Area Based on Both Quantitative and Qualitative Characters North West Tigray, Shire, Ethiopia","authors":"Bedasa Mekonnon","doi":"10.3923/IJPBG.2014.205.213","DOIUrl":"https://doi.org/10.3923/IJPBG.2014.205.213","url":null,"abstract":"","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"158 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132451628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genetic diversity of tannia (Xanthosoma sagittifolium (L.) Schott) genotypes using multivariate analysis at Jimma, Southwest Ethiopia.","authors":"Solomon Fantaw, A. Nebiyu, Tewodros Mulualem","doi":"10.3923/IJPBG.2014.194.204","DOIUrl":"https://doi.org/10.3923/IJPBG.2014.194.204","url":null,"abstract":"","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116871387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Ilodibia, N. Okeke, U. Achebe, T. P. Egboka, M. Chukwuma
{"title":"Plant Breeding for Food Security Sustainability and Industrial Growth","authors":"C. Ilodibia, N. Okeke, U. Achebe, T. P. Egboka, M. Chukwuma","doi":"10.3923/IJPBG.2014.219.223","DOIUrl":"https://doi.org/10.3923/IJPBG.2014.219.223","url":null,"abstract":"","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"216 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126168202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Assessment of Genetic Variability, Genetic Advance, Correlation and Path Analysis for Morphological Traits in Sesame Genotypes","authors":"Y. Gidey, S. A. Kebede, G. T. Gashawbeza","doi":"10.3923/IJPBG.2013.21.34","DOIUrl":"https://doi.org/10.3923/IJPBG.2013.21.34","url":null,"abstract":"Sixty four sesame accessions were evaluated in 8x8 simple lattice design at Humera in Western Tigray, in 2013/14. The objectives of the study were to estimate the genetic variability, association among characters, and to estimate genetic divergence among the accessions. Analysis of variance revealed that there was highly significant (p<0.001) difference among the sixty four accessions for all the fourteen characters studied. Number of capsule per plant and seed yield per hectare recorded high genotypic coefficients of variation (GCV) and phenotypic coefficients of variation (PCV) but medium genotypic coefficients of variation (GCV) and high phenotypic coefficients of variation (PCV). The highest heritability value were for days to 50% flowering, plant height, height to first capsule and number of capsule per plant. Traits like, number of capsule per plan with high phenotypic coefficients of variation (PCV), genotypic coefficients of variation (GCV), heritability and genetic advance as a percent of mean. This indicates that these characters can be improved through selection than heritability estimates alone. Number of primary branches per plant showed positive significant phenotypic and genotypic correlation with grain yield. Genotypic ally, path coefficient analysis based on grain yield as a dependent variable revealed that length of capsule bearing zone, number of primary branches per plant and 1000 seed weight exerted positive direct effect on seed yield. Thus, improvement through direct selection rather than a lengthy crossing program and hybridization involving crossing of the accessions from different clusters would produce viable and a potential segregant population.","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120966467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. A. I. AboMostafa, Gehan G. A. Abo Zeid, Zeinab E. Ghareeb, E. Sarhan, M. Abbas
{"title":"Combined and Genetic Analysis for Multiple-disease Resistance to Chocolate Spot and Rust on Faba Bean Yield","authors":"R. A. I. AboMostafa, Gehan G. A. Abo Zeid, Zeinab E. Ghareeb, E. Sarhan, M. Abbas","doi":"10.3923/IJPBG.2014.181.193","DOIUrl":"https://doi.org/10.3923/IJPBG.2014.181.193","url":null,"abstract":"","PeriodicalId":356916,"journal":{"name":"International Journal of Plant Breeding and Genetics","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122329184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}