Assessment of Negative Environmental Impacts of Drainage and Irrigation Canals on Ipomoea carnea Jacq. in Egypt

Abstract

This investigation demonstrates the effect of different environmental pollutants of drainage and irrigation canals on Ipomoea carnea Jacq. collected from ten different sites belongs to Dakahlia Governorate. This goal is achieved by using varietal tools as heavy metal analysis such as (Co2+, Cd2+, Pb2+, Cu2+ and Zn2+) of water, soil and plant leaves samples collected from each site, mitotic index, phase index, pollen abortion assay, protein profile using SDS-PAGE technique and molecular study using Random Amplified Polymorphic DNA (RAPD) technique. The effects of heavy metals on I. carnea induced a considerable changes in mitotic index and produced number of chromosomal abnormalities such as multinucleated cells at interphase, micronucleus at prophase, stickiness, non-congression, two groups, chromosome ring and disturbed at metaphase, late separation, laggard, bridge and diagonal in anaphase and telophase and reduced the frequency of pollen fertility (low stainability). The damage of DNA was reflected by changes in protein profile and DNA fingerprinting of I. carnea through the variation in bands by the appearance or disappearance of these bands.