{"title":"Disinfectant Effectiveness Against SARS-CoV-2 and Surrogates Using Cell Culture and RT-PCR","authors":"S. Bhavanam, M. Diggle, Jiaao Yu, X. Pang","doi":"10.11648/J.IJBSE.20210904.11","DOIUrl":"https://doi.org/10.11648/J.IJBSE.20210904.11","url":null,"abstract":"Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causes the global COVID-19 pandemic. Limited studies have been performed on various types of disinfectants utilized to control the spread of this highly contagious virus. This study aimed to investigate the inactivation of SARS-CoV-2 surrogate virus, hCoV-229E using an in vitro to test the anti-infectivity activity of the humidifier buffers (A and B, LumichemTM). A real-time reverse transcriptase quantitative PCR (RT-qPCR) assay was used to evaluate the effectiveness of these disinfectants on the degradation of viral RNA in a time dependent manner. The effects of disinfectants on viral infectivity were determined using a tissue culture infectious dose (TCID50) assay of a surrogate virus, hCoV-229E, in MRC-5 cell culture. The results demonstrated that the LumichemTM buffers A and B had a 2 to 3-log10 reduction inactivation using cell culture after a short exposure compared to the control, indicating the disinfection efficacy of the tested anti-infectivity compounds. The LumichemTM buffers A and B in addition did not affect the viral genomic RNA of a surrogate virus, hCoV-229E, thus representing an additional benefit with a negligible impact to operators and those in close contact when providing in-situ operational cleaning.","PeriodicalId":351050,"journal":{"name":"International Journal of Biomedical Science and Engineering","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132169392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dawei Wang, T. Yang, Xiang-yu Chen, Feng Guo, Liujun Jia, G. Yue, Yingkui Liang, Xin Wang
{"title":"Establishment and CT Imaging of Rabbits Abdominal Aorta Atherosclerosis Model Based on High-fat Diet and Balloon Strain Technique","authors":"Dawei Wang, T. Yang, Xiang-yu Chen, Feng Guo, Liujun Jia, G. Yue, Yingkui Liang, Xin Wang","doi":"10.11648/J.IJBSE.20210903.14","DOIUrl":"https://doi.org/10.11648/J.IJBSE.20210903.14","url":null,"abstract":"Background and Objectives: Atherosclerosis is the most common type of arteriosclerotic vascular disease. It is characterized by accumulation of lipids, hemorrhage and thrombosis, and gradual degeneration and calcification of the middle layer of the artery. It is very harmful to human body. To diagnose atherosclerosis at an early stage, a new animal model of abdominal aorta in New Zealand rabbits was established using high-fat diet with balloon injury to simulate the natural process of human disease. Methods: In our study, the high-fat diet and balloon strain technique were used to establish this model, CT imaging and pathological examination were used to prove the successful establishment of the model. Results: The results demonstrated that two weeks after high-fat feeding, the rabbits’ survival rate was 100% and their body weights gradually increased over time. Compared with basic levels, all atherosclerotic indexes (AI) were higher than 4. Pathological observation and CT imaging showed that the location of vascular injuries was stenosis and the lesions were consistent with the basic characteristics of atherosclerosis. Conclusions: The above results indicated that under our experimental conditions, the rabbits’ model of abdominal aorta atherosclerosis (AS) could be successfully reproduced. Compared with previous atherosclerosis models, it has the characteristics of a short modeling time and method simplicity. More importantly, it can be used as a follow-up model of atherosclerosis early diagnosis.","PeriodicalId":351050,"journal":{"name":"International Journal of Biomedical Science and Engineering","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126126472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Population Changes of Important Sucking-Pests Aphis gossypii (Glove.) and Bemisia tabaci (G.) in the New Varieties Second Plant (After Canolai) of Iran","authors":"M. Darvish, Zangi Mohammadreza","doi":"10.11648/J.IJBSE.20210903.13","DOIUrl":"https://doi.org/10.11648/J.IJBSE.20210903.13","url":null,"abstract":"Investigating the population dynamic of important cotton pests on new cotton varieties is important. Cotton cultivars have important pests such as thrips, aphids, whitefly and bollworm. Considering the premature cultivars with proper yield for second cultivation in cotton-growing Golestan province at the north of Iran, this study seems necessary. This genotypes was cultivated with 9 treatments in 4 replicates. A completely randomized block design was carried out in Hashem Abad research station in a spray-free plot with the dimensions of 30 × 80 cm in 2018-2019 years. According to the results of this study, analysis of variance of two years showed that the yield of N2G80 with 1538 g in the plot with 41.7% lint percentage had the highest yield, and M13 with 685 g in the plot and 35.8% lint percentage in the lowest yield they had. The infestation rate of the genotypes tested in the population of green aphid of cotton in K880-2 and Golestan® cultivars was 48.88 and 40.31 in aphid with the highest density, and the SKT-133 and TBL-60 genotype respectively with 27.15 and 29.11 aphids per leaf had the least infestation. The amount of infestation of the genotypes tested to the population of whitefly cotton on the N2G80 and TBL-60 genotype was 90.76 and 68.86 whitefly in the leaf with the highest infestation and N2G80 and TBL-60 genotype respectively with 40.05 and 75.51 white fly in leaf were the least infested. Correlation coefficient between traits was equal to 0.55. The number of bolls per plant was not correlated with other traits including boll weight, lint percentage, aphid and white fly.","PeriodicalId":351050,"journal":{"name":"International Journal of Biomedical Science and Engineering","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133437434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdulkadir Musliu, S. Adebisi, Samira Arzika, F. Oluwafemi
{"title":"Isolation and Identification of Pathogen Bacteria Associated with Farm Animals","authors":"Abdulkadir Musliu, S. Adebisi, Samira Arzika, F. Oluwafemi","doi":"10.11648/J.IJBSE.20210903.12","DOIUrl":"https://doi.org/10.11648/J.IJBSE.20210903.12","url":null,"abstract":"Farm animals’ meat contributes significantly to the daily protein intake of many individuals but can also be a source of foodborne illnesses especially under the conditions in which animals are handled, slaughtered, transported and sold. The emergence and re-emergence of diseases due to pathogenic bacteria are the key issue of the new pattern of food trades. A total of twenty-one samples (VIS, FSA, STS, MSA, URS, UDS, and PES) were collected from farm animals from Rugga settlement of Birnin Kebbi. Some of the samples were collected using sterile swab stick while urine and stool samples were collected in sterile containers and were taken immediately to Microbiology Laboratory Waziri Umaru federal Polytechnic. The samples were bacteriologically analyzed and the isolates were identified using biochemical tests such as (indole, coagulase, catalase, urease, gluctose, lactose, maltose, motility, Voges Proskauer and methyl red). Fourteen bacterial species were isolated and identified as pathogens from the sheep samples. They are Gram positive bacteria such as Streptococcus agalactiae and Staphylococcus aureus as well as Gram negative bacteria such as Escherichia coli and Shigella sp and Salmonella sp. The isolates were identified based on their reaction to biochemical tests. The findings revealed that sheep are potential vehicles for transmitting pathogenic bacteria and the presence of these microorganisms may lead to poisoning and can as well claim the live of the sheep from which they were isolated.","PeriodicalId":351050,"journal":{"name":"International Journal of Biomedical Science and Engineering","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127128104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kirtan Patel, Amber M. Maraccini, Timothy T. Grunert, Wei Yang, A. Slonim
{"title":"Exploring the Relationship Between Critical Access Hospitals and Rural County Health","authors":"Kirtan Patel, Amber M. Maraccini, Timothy T. Grunert, Wei Yang, A. Slonim","doi":"10.11648/J.IJBSE.20210902.13","DOIUrl":"https://doi.org/10.11648/J.IJBSE.20210902.13","url":null,"abstract":"Critical Access Hospitals (CAHs) were developed as a model to improve the access and availability of hospital services in rural counties. There has been limited research on clinical outcomes to evaluate the impact of CAHs since they were authorized through the Balanced Budget Act. This study evaluates CAH’s performance on clinical outcomes, and compares health outcomes between rural counties with CAHs and rural counties without established federally supported hospitals. The American Hospital Association’s (AHA) Annual Survey Database was used to identify CAHs within rural counties and their characteristics. The County Health Rankings (CHR) data were used to quantify health outcomes by county. US rural counties with CAHs versus remaining US rural counties without CAHs were correlated with measures of Clinical Care (p < 0.001). US rural counties with CAHs presented greater health status with regard to All Health Outcomes, p < 0.0001; Length of Life, p < 0.0001; Quality of Life, p < 0.0001; All Health Factors, p < 0.0001; Health Behaviors, p < 0.0001; Social and Economic Environment, p < 0.0001 and Physical Environment, p < 0.0001, than compared to US rural counties without CAHs. Rural counties serviced by CAHs demonstrate better overall health status scores, on several CHR metrics, as compared to rural counties without CAHs. The only exception to this conclusion being that rural counties without CAHs performed superiorly in the CHR metrics related to primary care and mental health services, demonstrating capacities in which CAHs could improve the impact on health in the counties they serve.","PeriodicalId":351050,"journal":{"name":"International Journal of Biomedical Science and Engineering","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131456079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Ayodele, Atoyebi Ebenezer Oluwatosin, Olutosoye Christian Taiwo, A. Dare
{"title":"Computational Fluid Dynamics Modeling in Respiratory Airways Obstruction: Current Applications and Prospects","authors":"O. Ayodele, Atoyebi Ebenezer Oluwatosin, Olutosoye Christian Taiwo, A. Dare","doi":"10.11648/J.IJBSE.20210902.12","DOIUrl":"https://doi.org/10.11648/J.IJBSE.20210902.12","url":null,"abstract":"Breathing conditions pertaining to nasal obstruction, obstructive sleep apnea, and airflow resistance in the human lower airways have been investigated extensively by researchers over the years. Due to the availability of advanced computer numerical models, such as computational fluid dynamics (CFD), researchers have made progressive studies of airflow characteristic, especially the effects of airflow pressure, velocity and wall shear stress in human obstructive airways. Studies utilizing CFD have enhanced clinical understanding of the physiology and pathophysiology of the respiratory system through the concept of three-dimensional models that facilitate airflow simulation. The main objective of this article is to review recent CFD literature on nasal airflow and lower airway obstruction. The review covers the role of segmentation threshold in the outcome of airflow simulation in the nasal cavity, and results of fluid structure interaction (FSI) and computational fluid dynamics in nasal obstruction and airway collapse in obstructive sleep apnea were also correlated. For models of the lower airways, we evaluated the effect of extra-thoracic airway (ETA) on downstream airflow during simulation against the popular Weibel’s model. In the concluding section, we discussed the advantages, limitations, and prospects (precisely with deep machine learning) of computational fluid dynamics in the clinical assessment and investigation of respiratory diseases.","PeriodicalId":351050,"journal":{"name":"International Journal of Biomedical Science and Engineering","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123136953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seema Rani Padhiary, Kalpana Priyadarsini Das, M. Bobde, V. Pooja, Sameer Sharma
{"title":"Pharmacological Analysis and Molecular Docking of Laurus nobilis (Bay Leaf) for Lung Cancer with Reference to Sirtuin Drug Targets","authors":"Seema Rani Padhiary, Kalpana Priyadarsini Das, M. Bobde, V. Pooja, Sameer Sharma","doi":"10.11648/J.IJBSE.20210902.11","DOIUrl":"https://doi.org/10.11648/J.IJBSE.20210902.11","url":null,"abstract":"The aim of this in silico study was to analyze the inhibitory activity of selected phytocompounds from the Bayleaf (Laurus nobilis) in contrast to sirtuin proteins using the various pharmacological tools and molecular docking analysis. Laurus nobilis is a perennial herbs native to the family Lauraceae and it has been cultivated throughout the tropical, European, subtropical, and Asian nations. It has been used for thousands of years for food flavoring, essential oil applications, and in traditional medicine. Mostly, it contains all types of secondary metabolites such as tannins, flavones, flavonoids, alkaloids, eugenol, linalool, methyl chavicol, and anthocyanins. The 3D structures of phytocompounds were retrieved from Pubchem and chemspider databases and subjected to various bioinformatic tools such as SwissADME, Modeller, and Autodock for molecular docking to predict the active binding sites of sirtuin proteins. The comparison of molecular docking score exposed that the targeted phytocompounds showed good binding affinity in contrast to anti-cancer sirtuin proteins. The ADME and Molecular docking properties for drug likeness making them significant agents for biological activities and it is expected to be beneficial and effective for cancer. Bayleaf shows an optimistic results towards the treatment of many diseases. The Bayleaf traditionally has healing properties which has now dragged the attention of science for the betterment of humans. The phytochemical compounds found in and taken in the above research have showed good results with cancer receptors Sirtuin1 and Sirtuin4.","PeriodicalId":351050,"journal":{"name":"International Journal of Biomedical Science and Engineering","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114914688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of a Leukocyte Removal Filter for Hepatitis C Therapy and the Possibility of Multipurpose Treatment","authors":"Y. Fueda, H. Kotera","doi":"10.11648/J.IJBSE.20210901.13","DOIUrl":"https://doi.org/10.11648/J.IJBSE.20210901.13","url":null,"abstract":"Hepatitis C virus infects and causes inflammation in extrahepatic organs and the liver. Patients with chronic hepatitis C disease receive interferon-based treatment, and both interferon and interferon-free therapies are expensive. We attempted to eliminate hepatitis C virus-infected cells using extracorporeal circulation with a unique filter. We created two types of the filters with syndiotactic polystyrene (SPS) resin sheets in a solution that dissolved hydroxypropyl cellulose (HPC) and cellulose acetate (CA) in oleyl alcohol, respectively. First, hepatitis C virus-positive cells were confirmed in the blood and mononuclear cells of hepatitis C virus-infected volunteers, showing that mononuclear cells phagocytosed the hepatitis C virus. Next, we extracted blood from three healthy volunteers and examined the blood cell removal rate using various filters. The HPC-SPS (fiber diameter, 3.5 μm) showed the highest mononuclear cell removal rate and the lowest platelet removal rate. HPC-SPS reduced hepatitis C virus-positive cells in patients with the hepatitis C virus, and the removal rate of monocytes containing the hepatitis C virus was 77.2%. Thus, HPC-SPS may serve as filter to remove mononuclear cells and granulocytes from blood. Although a technique using extracorporeal circulation is required to apply HPC-SPS, the technical cost is lower than IFN-free drug therapy. We believe that HPC-SPS can be utilized for the treatment of HCV- and leukocyte-related diseases.","PeriodicalId":351050,"journal":{"name":"International Journal of Biomedical Science and Engineering","volume":"142 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125903678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Significance of Platelet Count in Short-Term Prognosis of Type B Acute Aortic Dissection Patients","authors":"Wenming Shao, Zengxi Yao, Ya-jun Jiang","doi":"10.11648/J.IJBSE.20210901.12","DOIUrl":"https://doi.org/10.11648/J.IJBSE.20210901.12","url":null,"abstract":"Background: The lower number of platelet (PLT) has been found to be a risk factor in patients with type A acute aortic dissection (AAD) In admission. However prognostic implications of the PLT count in the type B AAD patients has not yet been elucidated. Methods: We consecutively enrolled 81 patients which confirmed with type B. Patients were divided into survival group and death group and PLT count were measured on admission. Univariate analysis and multiple logistic regression analysis were used to identify the predictors of in-hospital mortality. Results: Compared with the survival group, the death group PLT count was significant lower (172.07±57.38×109/L vs. 227.13±75.97×109/L, P 179.5 × 109 / L) and the low PLT count group (<179.5 × 109 / L) (P=0.001). Conclusions: Low PLT on admission count is one of the specific dead risk factors for type B AAD in-hospital patients.","PeriodicalId":351050,"journal":{"name":"International Journal of Biomedical Science and Engineering","volume":"251 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117289713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Analysis of Clinical Characteristics of Submucosal Tumor of Digestive Tract Under Endoscope","authors":"W. Ye, Zhaolin Chen","doi":"10.11648/J.IJBSE.20210901.11","DOIUrl":"https://doi.org/10.11648/J.IJBSE.20210901.11","url":null,"abstract":"Background: Submucosal tumors of the digestive tract are common diseases, which are difficult to diagnose and treat because of their occurrence in the digestive tract. In the past, follow-up observation or surgical operation were usually adopted for SMT, but the surgical operation had many disadvantages such as large trauma, many complications and long postoperative recovery time. In contrast, endoscopic therapy is characterized by safety. The clinical features of endoscopic mass resection remain unclear. Objective: To analyze the clinical features of submucosal masses of digestive tract. The pathological types and distribution of the tumor and the safety of endoscopic surgery were also discussedMethods: We analyzed the clinical data of 108 patients with submucosal tumors of the digestive tract by endoscopic surgery in our hospital. Results: The submucosal masses of the digestive tract were mainly leiomyoma and gastrointestinal stromal tumor, mostly benign lesions, mainly distributed in the mucosa and muscularis propria, and there was no difference between the sexes. The operative complication rate of endoscopic surgery for submucosal masses of digestive tract is low. Conclusion: c of small gastric submucosal tumors is safe enough and effective in diagnosis and treatment. EMR and ERE are still commonly used endoscopic surgery methods at present.","PeriodicalId":351050,"journal":{"name":"International Journal of Biomedical Science and Engineering","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128879202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}