农场动物致病菌的分离与鉴定

Abdulkadir Musliu, S. Adebisi, Samira Arzika, F. Oluwafemi
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摘要

农场动物的肉对许多人的每日蛋白质摄入量有重要贡献,但也可能是食源性疾病的来源,特别是在处理、屠宰、运输和销售动物的条件下。由病原菌引起的疾病的出现和再出现是食品行业新格局的关键问题。从Birnin Kebbi Rugga居民点农场动物共采集了21份样本(VIS、FSA、STS、MSA、URS、UDS和PES)。一些样本是用无菌棉签收集的,而尿液和粪便样本是在无菌容器中收集的,并立即送往瓦济里奥马鲁联邦理工学院微生物实验室。采用吲哚、凝固酶、过氧化氢酶、脲酶、葡萄糖、乳糖、麦芽糖、活力、Voges Proskauer和甲基红等生化试验对样品进行了细菌学分析和鉴定。从绵羊样本中分离鉴定出14种病原菌。它们是革兰氏阳性菌,如无乳链球菌和金黄色葡萄球菌,以及革兰氏阴性菌,如大肠杆菌、志贺氏菌和沙门氏菌。这些分离菌是根据它们对生化试验的反应鉴定的。研究结果表明,羊是传播致病菌的潜在媒介,这些微生物的存在可能导致中毒,也可能夺走被分离出致病菌的羊的生命。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and Identification of Pathogen Bacteria Associated with Farm Animals
Farm animals’ meat contributes significantly to the daily protein intake of many individuals but can also be a source of foodborne illnesses especially under the conditions in which animals are handled, slaughtered, transported and sold. The emergence and re-emergence of diseases due to pathogenic bacteria are the key issue of the new pattern of food trades. A total of twenty-one samples (VIS, FSA, STS, MSA, URS, UDS, and PES) were collected from farm animals from Rugga settlement of Birnin Kebbi. Some of the samples were collected using sterile swab stick while urine and stool samples were collected in sterile containers and were taken immediately to Microbiology Laboratory Waziri Umaru federal Polytechnic. The samples were bacteriologically analyzed and the isolates were identified using biochemical tests such as (indole, coagulase, catalase, urease, gluctose, lactose, maltose, motility, Voges Proskauer and methyl red). Fourteen bacterial species were isolated and identified as pathogens from the sheep samples. They are Gram positive bacteria such as Streptococcus agalactiae and Staphylococcus aureus as well as Gram negative bacteria such as Escherichia coli and Shigella sp and Salmonella sp. The isolates were identified based on their reaction to biochemical tests. The findings revealed that sheep are potential vehicles for transmitting pathogenic bacteria and the presence of these microorganisms may lead to poisoning and can as well claim the live of the sheep from which they were isolated.
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