Nigerian Journal of Physiological Sciences最新文献

{"title":"Comparative study on effects of Cymbopogon citratus (DC.) Stapf and Mangifera indica (L.) on sperm indices of male albino rats.","authors":"Solomon Beshel Solomon Beshel, Joseph Ekpenyong Joseph Felix, Moses Daben Rindum, Peace Chinedu-Ndukwe Amarachi, Peter Adie Imbufe, Godfrey Asuquo Okon-Nyong, Edwin Okon Anoyom","doi":"10.54548/njps.v40i2.11","DOIUrl":"10.54548/njps.v40i2.11","url":null,"abstract":"<p><p>The effects of various doses of Cymbopogon citratus (DC.) Stapf and Mangifera indica (L.) ethanolic leaf extract was investigated on fertility of male albino rats. Twenty-five (25) adult male rats were randomly grouped into five (5) cages (n=5). Control rats were given distilled water while experimental groups were daily administered ethanol leaf extract of C. citratus and M. indica through oral gavage at 150 and 300 mg/kg body weight, respectively; for 21 days. After the 21st day of post exposure (PE), experimental animals were sacrificed and their testes extracted and fixed. Sperm count, sperm morphology and sperm motility were assessed. C. citratus extract significantly increased mean sperm count of the treated rats at both 150 mg/kg body weight (272.00±7.47x106/ml) and 300 mg/kg body weight (297.00±3.62) groups, compared to those of M. indica extract (113.20±12.51x106/ml) and (27.60±3.67x106/ml) for 150 and 300 mg/kg body weight, respectively. Also, C. citratus produced a reduction in abnormal sperm morphology (15.20±1.77%) and (17.00±2.00%) for 300 and 150 mg/kg body weight, respectively; compared to M. indica extract (64.00±1.87%) and (51.00±3.32%) for 300 and 150 mg/kg body weight, respectively. M. indica significantly (p<0.05) reduced sperm motility while increasing sperm morphology indices of rats in all doses. However, C. citratus extract produced significant increase in sperm motility and a corresponding decrease in sperm morphology across the dose groups compared to M. indica extract. C. citratus boosted sperm count while M. indica decreased sperm count.  The findings in this research indicated that C. citratus may influence fertility by stimulating sperm count production than M. indica, compared with control, and this effect is dose-dependent. Keywords: Cymbopogon citratus, Mangifera indica, human fertility control, contraceptives, family planning, sperm indices.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":"40 2","pages":"303-309"},"PeriodicalIF":0.0,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147729868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pretreatment with vincristine attenuates haematological and plasma biochemical alterations in isoprenaline treated rats.","authors":"J N Asiwe, D E Okonofua, B Ben-Azu, J C Orji, A A Fasanmade","doi":"10.54548/njps.v40i2.13","DOIUrl":"10.54548/njps.v40i2.13","url":null,"abstract":"<p><p>Sustained adrenergic stimulation is linked to altered plasma biochemistry and haematological functions. However, whether vincristine, a vinca alkaloid with acclaimed cardioprotective property, can protect against biological alteration remains unknown. Hence, this present study investigated the protective impact of vincristine against plasma biochemical alteration caused by sustained beta-adrenergic stimulation in male Wistar rat. Animals were randomly divided into four groups; Group 1 served as normal control and was treated with saline (10 mL/kg), Group 2 received isoprenaline (ISO) (1 mg/kg) group daily for 14 days, group 3 was treated with ISO (1 mg/kg) and vincristine (25 µg/kg) simultaneously for 14 days while group 4 was pre-treated with vincristine (25µg/kg) for 14 days before exposure to isoprenaline. All treatment was done intraperitoneal. Following euthanasia, extract plasmas were used for haematological and plasma biochemical assays. Our results showed a significant erythrocytosis, haematocrit, haemoglobinemia and leucocytosis while there was a decreased in lymphocyte and thrombocyte in ISO-treated animals when compared with the control animals. However, VCR pre-treatment reversed these haematological parameters to normal levels. Liver (ALP, AST and ALT) and kidney (Creatinine, BUN, globulin, albumin and total protein) function markers were greatly altered in ISO-treated animals. However, pretreatment with VCR significantly modulated these markers. Plasma electrolytes were also modulated by VCR pre-treatment against the derangement caused by ISO treatment. Finally our study suggest that vincristine pre-treatment protected animals exposed to sustained β-adrenergic stimulation from haematological, liver and kidney function alterations.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":"40 2","pages":"321-328"},"PeriodicalIF":0.0,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147730130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methanol Leaf Extract of <i>Azadirachta Indica</i> Attenuates Lead Acetate-Induced Cardiorenal Toxicity in Rats.","authors":"Temitayo AdeyeOluwa, Ademola Oyagbemi, Adeolu Adedapo, Anofi Ashafa, Temidayo Omobowale","doi":"10.54548/njps.v40i2.8","DOIUrl":"10.54548/njps.v40i2.8","url":null,"abstract":"<p><p>The toxic effect of lead on heart and kidney tissues has been associated with its ability to cause oxidative stress. Azadirachta indica is a versatile medicinal plant with diverse pharmacological potentials. The ameliorative effect of the methanol extract of Azadirachta indica leaf (MEAI) was studied on male Wistar rats exposed to lead acetate (PbAc). Preliminary assays were carried out to assess the total phenolic, total flavonoid, and total flavonol contents of the extract using quantitative phytochemical assays. Cytotoxicity was tested on Vero cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide [MTT] assay. Wistar rats were grouped into control, toxicant, and treated groups and administered distilled water, 0.1/0.2% PbAc, and 0.1/0.2% PbAc + 100/200 mg/kg MEAI respectively. Results showed that the total phenolic content of MEAI was negligible while the flavonoid (300±0.12 mg Quercetin/g) and flavonol (80±0.06 mg Rutin/g) contents were significant. LC50 value of MEAI was 0.05 mg/mL. MEAI significantly (p<0.05) increased weight, reduced blood pressure, and increased blood flow/volume in the treated groups. There was significant (p<0.05) reduction in hydrogen peroxide (H2O2) and significant (p<0.05) increase in Nitric oxide (NO) in some of the treated groups. Histopathology revealed that MEAI reduced the infiltration of inflammatory cells in the heart and kidney tissues of the treated groups when compared to those of the lead-exposed untreated groups. There were variations in the expression of cytochrome C in the heart and kidney tissues of the experimental groups compared with the control. This study demonstrates MEAI as a potential drug candidate in the amelioration of lead acetate-induced cardiorenal toxicity by inhibition of oxidative stress, inflammation and by regulating apoptosis.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":"40 2","pages":"271-282"},"PeriodicalIF":0.0,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147730036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Some Genetic thrombophilias in Nigerian patients with Venous Thromboembolism.","authors":"Eunice Oluwaseyi Bamisaye, Sunday Peter Ogundeji, Tolani Ojo, Evarista O Osime","doi":"10.54548/njps.v40i1.6","DOIUrl":"https://doi.org/10.54548/njps.v40i1.6","url":null,"abstract":"<p><p>Venous thromboembolism (VTE) is a leading cause of mortality globally, resulting from genetic risk factors and/or acquired risk factors like oral contraceptives, smoking, diabetes, immobilization, cancer, trauma, fracture, and surgical procedures. This study aims to assess the involvement and prevalence of some genetic thrombophilias in Nigerian VTE patients. A total of 107 participants were recruited, comprising 67 individuals with VTE from three tertiary hospitals in Southwest Nigeria and 50 apparently healthy controls. Parameters included the absolute platelet count, prothrombin time (PT), activated partial thromboplastin time (APTT), D-dimer, protein C, protein S, and antithrombin levels. The VTE patients had significantly lower mean platelet counts, protein C and S antigenic concentrations, and protein S activity (p<0.05), with elevated mean PT and D-dimer levels (p<0.05). Protein C antigen and activity levels were reduced in 6.8% and 2.5% of participants, respectively, indicating deficiencies, while protein S antigen and activity were reduced in 1.7% and 0.9%. One (0.9%) participant had reduced antithrombin III level. Seven participants with protein S or C deficiencies experienced recurrent thrombosis. The study identifies type I antithrombin III deficiency and types I and II protein C and S deficiencies as genetic risk factors for VTE. The prolonged PT and elevated D-dimer levels observed in this study challenged the assumption of consistently reduced PT in VTE patients.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Effects of Persea americana L. Phytochemicals on Key Molecular Targets in Knee Osteoarthritis: An In-silico and In-vivo approach.","authors":"Olabode Akintoye, Olabode Akintoye, Adesola Adedotun Oniyide, Christopher Oloruntoba Akintayo, Olusegun Adebayo Adeoluwa, Kehinde Samuel Olaniyi","doi":"10.54548/njps.v40i1.21","DOIUrl":"https://doi.org/10.54548/njps.v40i1.21","url":null,"abstract":"<p><p>Knee osteoarthritis (KOA) is a prevalent degenerative joint disorder characterized by chronic inflammation, cartilage degradation, and altered bone remodelling. This study investigated the inhibitory effects of Persea americana L. phytochemicals on key molecular targets involved in KOA pathogenesis and compared them with clinically used drugs targeting these pathways. Specifically, Interleukin receptor-associated kinase 4 (IRAK4), Transforming Growth Factor-Beta (TGF-β), Microsomal Prostaglandin E Synthase 1 (mPGES1), Kelch-like ECH-Associated Protein 1 (Keap1), Carbonic Anhydrase 1 (CA1), and Collagen II. Molecular docking analyses were performed using Schrodinger (Maestro 12.12). All proteins' 3D X-ray crystallographic structures were screened based on the following properties (Organism, Expression system, and Resolution) using Protein Data Bank (RCSB PDB) https://www.rcsb.org/. Various lead compounds with significant binding affinities to these targets have been identified, outperforming conventional pharmacological agents. In vivo assessments using the hot plate test were conducted on 30 male Wistar rats, which were divided into six experimental groups. Chemical induction of KOA was performed by intra-articular injection of 25uL of saline dissolved 4 mg/kg of Sodium monoiodoacetate (MIA) in four of five groups: Control, Sham, and 3 treated with Ibuprofen, Low, and High Persea americana L. extracts, respectively, except the KOA only group. The docking scores from all the pathways showed higher binding energy when compared to the present drug samples, except for KEAP 1, where Chlorhexidine \"STD\" and Quercetin had binding scores of -6.576 and -6.557, respectively. Persea americana L. extracts treated rats showed significantly enhanced pain thresholds in KOA models compared to pathological untreated and ibuprofen-treated groups (p<0.006; 0.041 respectively) by Day 21 post-induction of KOA, indicating their analgesic efficacy. These results collectively highlight the potential of Persea americana L. phytochemicals as novel therapeutic agents for the management of knee osteoarthritis, with strong consideration of a systemic pharmacological approach.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":"40 1","pages":"179-189"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hesperidin nanoparticles prevent scopolamine-induced cognition impairment through amplification of antioxidant defense system and cholinergic neurotransmission in mice.","authors":"Ismail O Ishola, Khadijat Olabisi Abdulwahab, Taiwo Grace Olubodun-Obadun, Ibukunoluwa Elizabeth Oluwatimilehin, Temitope Inioluwa Agbaje, Oluwatoyin Esther Agbaje","doi":"10.54548/njps.v40i1.14","DOIUrl":"https://doi.org/10.54548/njps.v40i1.14","url":null,"abstract":"<p><p>The rapid increase in aging population and age-linked cognitive impairment as well as dementia of Alzheimer's type are becoming more prevalent globally. Genetic and environment interactions played key role in dementia pathology. Oxidative stress and cholinergic disruptions are well linked with dementia. Hence, phytochemicals with neuroprotective and antioxidant properties could help ameliorate mitochondrial dysfunction and toxic effects misfolded amyloid-beta and tau proteins in dementia of Alzheimer's type. Previous studies have alluded to the beneficial action of hesperidin in mild cognitive impairment but delivery could be better enhanced in nanoparticulate form. Hence, this study sought to investigate the memory enhancing ability of hesperidin nanoparticles (HES_) on scopolamine-induced memory impairment in mice. Mice were randomly assigned into 6 groups (n=6) and treated as follows; vehicle only, vehicle + SCOP (1mg/kg, i.p.), HES (1,10 and 50mg/kg, p.o., respectively) + SCOP and donepezil (1mg/kg; p.o.) + SCOP for 14 consecutive days followed by behavioral assessment for memory function using open field test, Y-maze, novel object recognition and Morris water maze for locomotion, working, cognition and spatial learning, respectively. The animals were euthanized and brain samples were collected for biochemical assays (oxidative stress markers and acetylcholinesterase activity).   SCOP or HES administration did not affect locomotor activity, however, SCOP reduced the percentage alternation behaviour in the Y-maze and discrimination index in NOR tests with no significant change in escape latency time in MWM task indicative of working memory, cognition and spatial learning impairment. In contrast, the pre-administration of HES produced a dose-dependent and significant increase in working memory, cognition and spatial learning abilities. Similarly, HES_ pretreatment reduced scopolamine-induced increase in lipid peroxidation and acetylcholinesterase activity and deficit in antioxidant enzyme activity in the hippocampus and prefrontal cortex caused by SCOP. The results of the present study further showed the potential of hesperidin in nanoparticle form in the enhancement of memory formation through the amplification of antioxidant defense and cholinergic neurotransmission.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":"40 1","pages":"119-126"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal Environmental Temperature During Gestation Affects Renal Function Indices and Oxidative Stress in The Kidney of Offsprings of Wistar Rats.","authors":"Sikirullai Olatunde Jeje, P O Mosisiyenome, Ayomide E Olowoyo, T P Omayone, T G Adedeji, F K Ola-Mudathir","doi":"10.54548/njps.v40i1.12","DOIUrl":"https://doi.org/10.54548/njps.v40i1.12","url":null,"abstract":"<p><p>  Maternal exposure to increase temperature reduces birth weight. Low birth weight is related to adult-onset of non-communicable diseases. In this study, the effects of maternal exposure to various environmental temperatures during gestation on renal function and oxidative stress indices in offspring of Wistar rats were assessed. Fifteen pregnant Wistar rats were divided into 3 groups (n=5). The animals were housed in specialized thermoregulatory cages at 250C, 320C and 390C respectively. The animals were allowed to deliver spontaneously. At 12 weeks of age, the offspring were euthanized. Urea concentrations in seru, urine and creatinine clearance were assessed as renal function indices. Renal superoxide dismutase (SOD), catalase and malondialdehyde (MDA) activities were estimated as indices of oxidative stress. Histopathology and histomorphometry of the kidney were also assessed. Concentrations of urine creatinine and creatinine clearance of temperatureup 39°C were significantly higher than that of the 250C and 320C groups in both male and female offspring. Catalase and SOD activities in kidneys of the male offspring were significantly decreased in temperature group 320C when compared to temperature groups 250C and 390C. Meanwhile, MDA level was significantly higher in the320C group.In the female offspring, exposure to 250C significantly increased the concentration of serum creatinine, urea and renal MDA levels compared to other temperature groups. However, SOD activities were lowered in the 250C group. In conclusion, maternal environmental temperature during gestation affects renal function and oxidative stress indices in offspring of Wistar rats. These effects may be affected by sex of the offspring.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":"40 1","pages":"101-108"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Educational and Family Factors in Child Autism: A Systematic Literature Review.","authors":"Jasim Mohammed Dahr, Yaghoub Farjami","doi":"10.54548/njps.v40i1.3","DOIUrl":"https://doi.org/10.54548/njps.v40i1.3","url":null,"abstract":"<p><p>The early detection of autism spectrum disorders is considered the best strategy for managing, controlling, and intervening with the patients before they reach adulthood. There is a consensus among medical professionals and research communities about the mismatching criteria, confusing symptoms, and inexhaustive nature of symptoms. Also, there are no established diagnostic factors to date due to many more non-deterministic factors like gender, race, age and region. In this study, a systematic literature review (SLR) was done on educational and family factors affecting child autism spectrum disorder at early stages. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were adopted for selecting the forty-eight (48) items for the study. The results of this study revealed the educational and family factors for children with autism. This, in turn, will assist medical practitioners during the screening process, as well as help schools and specialised centres for autism plan effectively to meet the needs of integrated educational services for children with autism. This study also included a set of educational tools related to managing children with autism. This reveals some requirements in the field of assistive software, especially in the commencement of AI assistive tools for helping better and effective education in ASD kids. In addition to future research directions and areas of additional studies related to autism.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":"40 1","pages":"17-29"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Global Perspective of Human Metapneumovirus.","authors":"S O Ayanwale, R A Audu, S A Omilabu, Simeon I B Cadmus","doi":"10.54548/njps.v40i1.2","DOIUrl":"https://doi.org/10.54548/njps.v40i1.2","url":null,"abstract":"<p><p>Globally, human metapneumovirus (hMPV) constitutes a more significant proportion of acute respiratory tract infections (ARTIs), which are a primary cause of morbidity and mortality in children. A range of viruses, such as influenza viruses, respiratory syncytial virus (RSV), picornaviruses, coronaviruses, parainfluenza viruses, and adenoviruses, have been linked to various respiratory syndromes across all age categories. Notably, some of these viruses circulate and co-infect individuals, increasing the possibility of resulting in complex interactions that may affect disease severity, immune response, and epidemiology. It is also known that the majority of children contract hMPV by the age of 5, with the most severe cases found in infants, comprising both symptomatic and asymptomatic infections. Although hMPV is widely common, there are currently no approved vaccines or specific antiviral therapies available, highlighting the need for more research into focused treatments and vaccine development. Improvements in molecular diagnostics have increased detection rates, though difficulties persist in disease monitoring and management. The 2024-2025 current outbreak in China commenced in December 2024 and has experienced a rapid rise in cases, especially among children aged 14 and younger. Additionally, other countries like the United Kingdom (UK), France, and Germany have reported cases of the virus, reflecting its extensive spread. Consequently, an understanding of the epidemiology of hMPV is essential for creating effective targeted interventions. This review provides a global perspective of hMPV and points out knowledge gaps to drive future research initiatives in its management, prevention, and control.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":"40 1","pages":"9-16"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Evaluation of the Genetic Variability of Tilapia guineensis Populations Based on Microsatellite Markers.","authors":"A B Usman, O T Agbebi","doi":"10.54548/njps.v40i1.9","DOIUrl":"https://doi.org/10.54548/njps.v40i1.9","url":null,"abstract":"<p><p>Improvement of aquaculture production, conservation and stock management of T. guineensis species are important. The genetic variation of this specie as well as their cross-breeding pattern and growth performance were assessed. One hundred and fifty live samples with average weights of TG (21.90g and 31.60g) for females and males, respectively, were collected from three different water bodies. DNA was extracted using the phenol-chloroform isoamyl alcohol method from randomly selected fish populations. The quality and quantity of DNA were determined using a spectrophotometer. Microsatellites were amplified, and PAGE electrophoresis was analyzed using PopGene version 3.6 software. The DNA concentrations ranged from 100.36 ng/l to 3889.40 ng/l with purity values of 1.69 to 2.00. The partial regions of the gene fish populations amplified were polymorphic, with an average of two allele differences in frequency. The values of gene diversity, Polymorphic information Content and inbreeding coefficient were 0.41, 0.30, and -0.41, respectively. Observed heterozygosity was higher than expected heterozygosity in the fish populations. The genetic variations observed among the crossbreeds emphasized the importance of selective breeding for fast growth by increasing productivity, but pure lines should be maintained so that it forms part of the base line population and avoid inbreeding over time.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":"40 1","pages":"79-83"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}