Nigerian Journal of Physiological Sciences最新文献

{"title":"Cement Dust Exposure Alters Secretory Functions, Biochemical Profile and Morphology of Gastrointestinal Tissues in Rats Exposed to Cement Dust.","authors":"Mathew Wasiu Owonikoko, Adeola Temitope Salami, Olugbenga Adeola Odukanmi, Benjamin O Emikpe, Samuel Babafemi Olaleye","doi":"10.54548/njps.v37i2.4","DOIUrl":"10.54548/njps.v37i2.4","url":null,"abstract":"<p><p>Asides direct gastrointestinal exposure, inhalation route is another major xenobiotic exposure pathway to the gastrointestinal tract via mucociliary escalator. This triphasic study assesses cement dust inhalatory exposure effect on the possible alterations of the gastrointestinal tissues and secretion. 72 male, sixteen (16) weeks old Wistar rats were randomized into 3 different phases of 24 animals. Each phase comprised of 3 group of 8 animals. Group 1 (control) were sham-operated with clean ambient air, group 2 (14-days exposed) were exposed to cement dust for 14days, and group 3 (28-day exposed) were exposed to cement dust for 28 days. Biochemical indices including superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), sulfhydryl group, carbonyl group, Na+-K+ATPase pump activity, Nitric oxide (NO) were investigated spectrophotometrically in gastric and hepatic tissues while histopathology was studied using standard procedure. There was significant increase in the level of MDA, NO and carbonyl- an observation that contrasts with the level of CAT, SOD and sulfhydryl; no significant difference in Na+-K+-ATPase pump was observed in the exposed groups compared with control. Histopathological alterations in salivary gland and gastric tissues includes edema, inflammatory cell infiltration and vascular congestion. There was significant alteration in basal salivary, gastric and biliary secretions; increased stimulated salivary and gastric secretion via cholinergic stimulation. Conclusively, histopathological and spectrophotometric analyses reflect that inhalatory experimental exposure to cement dust significantly alter gastrointestinal secretions and predisposes the gastrointestinal tract to an array of deleterious effects via protein oxidation and antioxidant depletion and tissue peroxidation.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":" ","pages":"185-194"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44615357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does HAART dysregulate angiogenesis in HIV infected preeclampsia?","authors":"Sayuri Padayachee,&nbsp;Nalini Govender,&nbsp;Thajasvarie Naicker","doi":"10.54548/njps.v36i2.4","DOIUrl":"https://doi.org/10.54548/njps.v36i2.4","url":null,"abstract":"<p><p>A dysregulation of angiogenic mediators has been implicated in HIV infection. Inconsistent data exists on highly active antiretroviral therapy (HAART) usage in pregnancy and its association with PE development. In view of the high prevalence of HIV infection and PE in SA, this study was aimed at determining PlGF and sFlt-1 levels in HIV-infected normotensive and preeclamptic pregnancies treated with HAART. Both PlGF and sFlt-1 were quantified in serum from HIV positive [normotensive (N+) and preeclamptic (P+)]; and HIV negative [normotensive (N-) and preeclamptic (P-)] pregnancies, using a Milliplex Multiplex immunoassay. sFlt-1 was significantly upregulated in P+ vs the N+ groups. PlGF was significantly downregulated in PE vs normotensive groups, regardless of HIV status. sFlt-1/PlGF ratio was significantly increased in PE- vs the N- groups. We report an amplification of sFlt-1 in lieu of PlGF down-regulation in HIV-infected pregnancies receiving HAART .</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":" ","pages":"29-34"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40707525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skull Shape Variations in the Eidolon helvum (African Fruit Bat) Based on Geographical Location.","authors":"Olumayowa Igado,&nbsp;Jemima Joannis","doi":"10.54548/njps.v37i1.13","DOIUrl":"https://doi.org/10.54548/njps.v37i1.13","url":null,"abstract":"<p><p>The shape and size of a skull provides insight into the age, breed and gender of the animal. Skull shape variations have been reported in different animals, with some theories linking these variations to evolution and/or migration. This study assessed the variations observed in the skull shape, size and gross morphometrics of two groups of the Eidolon helvum obtained from two geographical regions in Nigeria (south and north). All skulls were rostro-caudally elongated, having a dolichocephalic appearance. The skulls from the north had a distinct dome shape, with a more prominent zygomatic process, absence of a 'diastema' and an extra upper molar, while the southern skulls showed a more dorsally flattened skull and a less prominent zygomatic process. The shape of the sagittal crest was different in the two groups, while there was the presence of an accessory infraorbital foramen in some of the southern skulls. The southern skulls lacked the palatine foramen. The lacrimal foramen was observed to be more caudally placed in the southern skulls. Values for most linear measurements were higher in the northern skulls, although statistically significant difference was not present in all. The value for the neurocranial volume was considerably higher in the northern skulls (4.41 ± 0.28 mls) relative to the southern skulls (2.0 ± 0.27 mls). Statistically significant differences were not observed between males and females (within regions). Data obtained from this study may find application in evolution and migration studies, wildlife medicine and surgery and comparative and forensic anatomy.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":" ","pages":"101-111"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40600510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in Serum Electrolytes, Urea and Creatinine in Nicotiana tabacum-treated Rats.","authors":"Azubuike Raphael Nwaji, Uduak Inwang, Favour-Ann Nwoke, Iniobong Ante","doi":"10.54548/njps.v37i1.19","DOIUrl":"10.54548/njps.v37i1.19","url":null,"abstract":"<p><p>Tobacco, which is a product of Nicotiana tabacum, has nicotine as its principal phytochemical. Nicotine has been reported to be an addictive drug and the leading cause of tobacco addiction worldwide with consequent renal implications. Therefore, this study was conducted to investigate the effect of the aqueous extract of Nicotiana. tabacum on serum electrolytes, urea and creatinine levels as indices of renal function in male rats. A total of 18 male wistar rats weighing 140-230g were used for this study. The animals were randomly divided into three groups (A, B and C), containing 6 rats each. Group A served as control while Group B and C were orally administered sublethal doses of 20 and 30 mg/kg body weight of the Nicotiana. tabacum extract respectively once per day for three weeks. At the end of the experimental period, all the animals were sacrificed. Blood samples were collected for biochemical assay. The results showed a significant increase (p<0.05) in the serum concentration of sodium, potassium and urea levels of rats treated with the extract when compared with the control. However, serum concentrations of chloride, bicarbonate and creatinine showed no significant appreciable differences between the treated groups and the control group (p<0.05). In conclusion, the study showed that aqueous extract of Nicotiana tabacum is associated with renal dysfunction with consequent hypernatremia and hyperkalemia, and may also suggest impaired urea clearance by the kidneys in male wistar rats.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":" ","pages":"153-156"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40597242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Angiotensin converting enzyme inhibitor captopril prevents neuronal overexpression of amyloid-beta and alpha-synuclein in Drosophila melanogaster genetic models of neurodegenerative diseases.","authors":"Ismail Ishola,&nbsp;Olasunmbo Afolayan,&nbsp;Adedeji Badru,&nbsp;Taiwo Olubodun-Obadun,&nbsp;Nkechi John,&nbsp;Olufunmilayo Adeyemi","doi":"10.54548/njps.v37i1.3","DOIUrl":"https://doi.org/10.54548/njps.v37i1.3","url":null,"abstract":"<p><strong>Background: </strong>Parkinson disease (PD) and Alzheimer's disease (AD) are progressive neurodegenerative disorders characterized by loss of selective neurons in discreet part of the brain. The peptide angiotensin II (Ang II) plays significant role in hippocampal and striatal neurons degeneration through the generation of reactive oxygen species. Blockade of the angiotensin converting enzyme or ATI receptors provides protection in animal models of neurodegenerative diseases. In the present study, the neuroprotective effect of captopril was investigated in Drosophila melanogaster model using the UAS-GAL4 system to express the synuclein and Aβ42 peptide in the flies' neurons.</p><p><strong>Methods: </strong>The disease causing human Aβ42 peptide or α-syn was expressed pan-neuronally (elav-GAL4) or dopamine neuron (DDC-GAL4) using the UAS-GAL4 system. Flies were either grown in food media with or without captopril (1, 5, or 10µM). This was followed by fecundity, larva motility, negative geotaxis assay (climbing) and lifespan as a measure of neurodegeneration.</p><p><strong>Results: </strong>Elav-Gal4<Aβ or DDC-GAL4<α-syn flies displayed significant decrease in larva motility when compared with normal control (w1118) which was reversed by the supplementation of the media with captopril (5 or 10 mM) indicative of neuroprotection. Interestingly, supplementation of flies' media with captopril improved climbing activity in Elav-Gal4<Aβ or DDC-GAL4<α-syn flies when compared with vehicle treated only. Moreover, flies grown on captopril caused no significant change in lifespan.  Conclusion: Findings from this study confirmed the neuroprotective action of captopril in genetic or familial forms of neurodegeneration.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":" ","pages":"21-28"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40600508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term acute constipation and not short-term acute diarrhea altered cardiovascular variables in male Wistar rats.","authors":"Adeola Odukanmi,&nbsp;O A Ajala,&nbsp;Samuel Babafemi Olaleye","doi":"10.54548/njps.v37i1.6","DOIUrl":"https://doi.org/10.54548/njps.v37i1.6","url":null,"abstract":"Gastrointestinal dysmotility is a substantial public health challenge globally. Based on previous findings in developed countries, it has been observed that there is an association between diarrhea, constipation, and some cardiovascular variables. This study investigated the effects of experimentally-induced short-term acute constipation and short-term acute diarrhea on certain cardiovascular variables in rats. Thirty (30) male Wistar rats (150 -180 g) were divided into three groups; Control, Diarrhoea, and Constipation. The experiment was carried out in 2 phases, the period after induction and the recovery period, and 5 animals per group were used for each phase. The control group received an equivalent amount of distilled water while Diarrhoea and the Constipation group were induced by oral administration of 2ml Castor oil and administration of Loperamide (3mg/kg, b.d, orally x 3 days), respectively. Cardiovascular variables were assessed using the Edan Scientific® Electrocardiography and  Heart Rate Variability machine. Recovery was allowed for 4 days after the onset of the procedure and cardiovascular parameters were reassessed. Post-induction Systolic Blood Pressure (SBP), Diastolic Blood Pressure (DBP), Mean Arterial Pressure (MAP) and Heart Rate (HR) significantly increased in constipated rats (153.2 ± 2.9 mmHg; 109.0 ± 3.7 mmHg;     123.7 ± 3.2 mmHg; 123.4±5.6 bpm) when compared with the control values (95.5±4.8 mmHg; 61.2 ± 3.5 mmHg; 72.6 ± 3.6 mmHg; 72.3 ± 5.2 bpm), respectively. The recovery SBP, DBP, MAP, and Heart Rate in the constipated group remained significantly higher compared to the control. Diarrhea had no significant effect on the parameters determined in both post-induction and recovery phases. The electrical activities did not change in both experimental groups compared to the control. This study revealed increased SBP, DBP, MAP, and HR in short-term acute constipated rats but not so with short-term acute experimental diarrhea.","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":" ","pages":"43-48"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40707526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect Of Caffeine and Adrenaline on Memory and Anxiety in Male Wistar Rats.","authors":"Aminat Imam-Fulani,&nbsp;Bamidele Victor Owoyele","doi":"10.54548/njps.v37i1.9","DOIUrl":"https://doi.org/10.54548/njps.v37i1.9","url":null,"abstract":"<p><p>The present study was designed to investigate the effects of caffeine and adrenaline administration on memory and anxiety in male rats. Rats weighing about 140-200g were used for the study. They were divided into three groups (4 animals per group). Study groups 1; a,b,c,d were healthy rats administered normal saline, 5,10 and 15mg/kg bw caffeine intraperitoneally (i.p.), respectively for 6 weeks. Study groups 2; a,b,c,d administered normal saline, 0.1, 0.2 and 0.31mg/kg bw adrenaline (i.p.), respectively for 6 weeks. Study groups 3; a,b,c,d administered normal saline, 5mg/kg caffeine (i.p.) + 0.1mg/kg adrenaline (i.p.), 10mg/kg Caffeine (i.p.) + 0.2mg/kg Adrenaline (i.p.) and 15mg/kg Caffeine (i.p.) + 0.3mg/kg Adrenaline (i.p.) respectively for 6 weeks. The result showed no significant difference in spatial memory across all animals in study groups 1: b,c,d when compared to control (a). Study groups 2: (b, c) showed increase in spatial memory when compared to control (a). 2(d) showed a significant (p<0.05) decrease. Study groups 3: b, c, d showed no significant difference in spatial memory when compared to control (a). Study groups 1: b, c showed significant (p<0.05) reduction in duration for the short and long term memory test when compared to control. Study groups 2 showed reduction in duration for both the long and short term memory test when compared to control Study groups 3 no significant (p<0.05) difference in short and long term memory test across all animals in the group. It was also observed that adrenaline enhanced short and long term memory and only high dose of adrenaline distorted spatial memory. Study groups 1; (b,c) showed significant (p<0.05) increase in the number of entries to the open arm of the elevated plus maze when compared to control (a). Study groups 2; (b), showed significant (p<0.05) increase in the frequencies of entries to the closed arm of the elevated plus maze when compared to control (a). Study groups 2; (d), showed a significant (p<0.05) decrease in the frequency of entries to the open and closed arm when compared to control (a). The study revealed that co- administration of caffeine and adrenaline led to elevation of mood, increased activity and reduction of anxiety in Wistar rats. In addition, it was observed that only high dose of adrenaline increased anxiety. It was also observed that caffeine and adrenaline enhanced short and long term memory and only high dose of adrenaline distorted spatial memory.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":" ","pages":"69-76"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40707521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gestational Nutrition as a Predisposing Factor to Obesity Onset in Offspring: Role for Involvement of Epigenetic Mechanism.","authors":"Sikirullai Olatunde Jeje,&nbsp;Michael Adenawoola,&nbsp;Christian Abosede","doi":"10.54548/njps.v37i1.1","DOIUrl":"https://doi.org/10.54548/njps.v37i1.1","url":null,"abstract":"<p><p>Maternal lifestyle has been implicated as a predisposing factor in the development of metabolic disorders in adulthood. This lifestyle includes the immediate environment, physical activity and nutrition. Maternal nutrition has direct influence on the developmental programming through biochemical alterations and can lead to modifications in the fetal genome through epigenetic mechanisms. Imbalance in basic micro or macro nutrients due to famine or food deficiency during delicate gestational periods can lead to onset of metabolic syndrome including obesity. A major example is the Dutch famine which led to a serious metabolic disorder in adulthood of affected infants. Notably due to gene variants, individualized responses to nutritional deficiencies are unconventional, therefore intensifying the need to study nutritional genomics during fetal programming. Epigenetic mechanisms can cause hereditary changes without changing the DNA sequence; the major mechanisms include small non-coding RNAs, histone modifications and most stable of all is DNA methylation. The significance association between obesity and DNA methylation is through regulation of genes implicated in lipid and glucose metabolism either directly or indirectly by hypomethylation or hypermethylation. Examples include CPT1A, APOA2, ADRB3 and POMC. Any maternal exposure to malnutrition or overnutrition that can affect genes regulating major metabolic pathways in the fetus, will eventually cause underlying changes that can predispose or cause the onset of metabolic disorder in adulthood. In this review, we examined the interaction between nutrition during gestation and epigenetic programming of metabolic syndrome.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":" ","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40597243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of Memory and Neurochemical Changes by Resveratrol and Environmental Enrichment in Rodent Model of Alzheimer's Disease.","authors":"M S Muhammad,&nbsp;J O Ayo,&nbsp;N M Danjuma,&nbsp;A AbdulWahab,&nbsp;A S Isa,&nbsp;A H Umar","doi":"10.54548/njps.v37i1.8","DOIUrl":"https://doi.org/10.54548/njps.v37i1.8","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is the most common cause of dementia that affects one patient every seven seconds, with over 35 million people currently affected worldwide. The aim of the study was to investigate the modulation of memory and neurochemical responses by resveratrol and environmental enrichment (EE) in aluminium chloride (AlCl3) model of Alzheimer's disease in mice. Male mice used for the study were divided into nine groups, of seven animals each. Group I (negative control): 0.2 ml normal saline/kg, Group II: 0.2 ml CMC/kg. Group III: resveratrol (200 mg/kg/), Group IV: CMC and kept in EE, Group V: AlCl3 at dose of 50 mg/kg, Group VI: resveratrol at dose of 200 mg/kg and kept in EE, Group VII: AlCl3 (50 mg/kg) + resveratrol (200 mg/kg), Group VIII: AlCl3 (50 mg/kg) and kept in EE, Group IX: AlCl3 (50 mg/kg) + resveratrol (200 mg/kg) and kept in enriched environment. All treatments were oral and lasted for 8 weeks. Assessments of memory was carried out before treatment, and at weeks 4 and 8, after the first treatment. The mice were sacrificed and hippocampal samples collected for neurochemical analysis. The findings of the study suggest that AlCl3 induced contextual fear memory deficit over time (p < 0.05), which was improved by resveratrol. Both Aβ and Nrf2 significantly (p < 0.05) increased in AlCl3 + EE + resveratrol group. In conclusion, Individual treatment with either resveratrol or EE improved memory over the combined treatment in AlCl3 model of AD by decreasing Aβ protein concentration.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":" ","pages":"59-67"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40600511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Luteolin normalizes blood pressure via its antioxidant activity and down-regulation of renal Angiotensin II receptor and Mineralocorticoid receptor expressions in rats co-exposed to Diclofenac and sodium fluoride.","authors":"Temitayo Ajibade,&nbsp;Akinleye Akinrinde,&nbsp;Moses Adetona,&nbsp;Ademola Oyagbemi,&nbsp;Aduragbenro Adedapo,&nbsp;Christopher Larbie,&nbsp;Temidayo Omobowale,&nbsp;Olufunke Ola-Davies,&nbsp;Adebowale Saba,&nbsp;Adeolu Adedapo,&nbsp;Oluwafemi Oguntibeju,&nbsp;Momoh Yakubu","doi":"10.54548/njps.v37i1.5","DOIUrl":"https://doi.org/10.54548/njps.v37i1.5","url":null,"abstract":"<p><p>This study was designed to investigate the modulatory role of Luteolin (Lut), a flavonoid phytochemical, on haemodynamic parameters and the potential mechanisms involving renal Angiotensin II (AT2R) and Mineralocorticoid (MCR) receptors in renal toxicity induced by co-exposure to Diclofenac (Dcf) and sodium fluoride (NaF) in rats.Male Wistar rats were administered with either vehicle (control), Dcf only (9 mg/kg orally) or concurrently with NaF (300 ppm in drinking water). Other groups were treated with LutA (100 mg/kg) or LutB (200 mg/kg) along with Dcf and NaF exposures. All treatments lasted 8 days, following which blood pressure indices were measured using tail-cuff plethysmography. Renal expressions of AT2R and MCR were studied with immunohistochemistry, while biomarkers of oxidative and antioxidant status were also measured in the kidneys. Systolic, diastolic and mean arterial pressures were significantly (p<0.05) reduced in Dcf-treated rats, compared to control values. However, co-treatment with NaF or Lut restored these parameters. While the expression of AT2R and MCR was high in the Dcf and Dcf+NaF groups, treatment with Lut caused obvious reduction in the renal expression of these receptors. Increased lipid peroxidation (Malondialdehyde) and protein oxidation (protein carbonyls) with a lowering of reduced glutathione levels contributed to the renal toxicity of Dcf, and these were significantly ameliorated in Lut-treated rats. In conclusion, the preservation of haemodynamic indices by Lutin the experimental ratsprobably included mechanisms involving down-regulation of renal expressions of AT2R and MCR, reduction of oxidative stress and an improvement of renal antioxidant status.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":" ","pages":"35-42"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40707524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}