Nigerian Journal of Physiological Sciences最新文献

筛选
英文 中文
Haematological and biochemical changes in arsenic trioxide-induced toxicity in Wistar rats treated with methanol extract of Parquetina nigrescens leaf. 用 Parquetina nigrescens 叶的甲醇提取物治疗三氧化二砷诱发的 Wistar 大鼠中毒的血液学和生化变化。
Nigerian Journal of Physiological Sciences Pub Date : 2022-12-31 DOI: 10.54548/njps.v37i2.11
Oore-Oluwapo Daramola, Wahab Adekunle Oyeyemi Oyeyemi, Adeniran Oluwadamilare Akinola Akinola, Yinusa Raji Raji
{"title":"Haematological and biochemical changes in arsenic trioxide-induced toxicity in Wistar rats treated with methanol extract of Parquetina nigrescens leaf.","authors":"Oore-Oluwapo Daramola, Wahab Adekunle Oyeyemi Oyeyemi, Adeniran Oluwadamilare Akinola Akinola, Yinusa Raji Raji","doi":"10.54548/njps.v37i2.11","DOIUrl":"10.54548/njps.v37i2.11","url":null,"abstract":"<p><p>This study was designed to investigate the phytochemical composition and protective effects of methanol extract of Parquetina nigrescens leaf (MEPL) in male Wistar rats. Phytochemical screening, in vitro antioxidant assay, gas chromatography-mass spectrometry (GC-MS) and LD50 were determined. Forty male Wistar rats were grouped into eight and orally treated for 54 days as follows: Group 1 (10% tween 80), Group 2 (3 mg/kg As2O3) Groups 3, 4 and 5 (250, 500 and 1000 mg/kg MEPL) and groups 6, 7 and 8, (250 mg/kg+As2O3, 500 mg/kg+As2O3 and 1000 mg/kg+As2O3). The animals were sacrificed on day 55 under anaesthesia. Blood was collected by cardiac puncture for heamatological studies. Liver concentrations of malondialdehyde (MDA), superoxide dismutase (SOD), aspartate aminotransferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP) activities were determined spectrophotometrically. Liver histology was also assessed. Flavonoids, tannin, alkaloids, saponin, and anthraquinone were present in MEPL, also, MEPL scavenged 2,2 diphenyl-1-picrylhydrazyl hydrate (DPPH) and Azino-bis-3-ethylbenzthiazoline-6-sulphonic acid radical (ABTS+). The IC50 of MEPL required to chelate metal was also low. The GC-MS revealed the presence of 24 essential oil. The LD50 was > 5000 mg/kg. Packed cell volume and red blood cell count were significantly reduced in 1000 mg/kg MEPL group, white blood cell count and SOD activity reduced (P<0.05) in 3 mg/kg As2O3 when compared with control but increased in groups co-treated with As2O3 and 250, 500 or 1000 mg/kg + As2O3. MDA concentration, AST, ALT and ALP activities increased significantly in 3 mg/kg As2O3 group but decreased (P<0.05) in groups co-treated with As2O3 and 250, 500 or 1000 mg/kg. The methanol extract of Parquetina nigrescens leaf in male Wistar rats has antioxidant, hepatoprotective and white blood cell protective effects.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":"37 2","pages":"235-246"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139503042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cholecalciferol (VD3) Attenuates L-DOPA-Induced Dyskinesia in Parkinsonian Mice Via Modulation of Microglia and Oxido-Inflammatory Mechanisms. 胆钙化醇(VD3)通过调节小胶质细胞和氧化应激炎症机制减轻帕金森病小鼠中左旋多巴诱导的运动障碍。
Nigerian Journal of Physiological Sciences Pub Date : 2022-12-31 DOI: 10.54548/njps.v37i2.3
Adedamola Bayo-Olugbami, AbdulRazaq Bidemi Nafiu, Abdulbasit Amin, Olalekan Michael Ogundele, Charles C Lee, Bamidele Victor Owoyele
{"title":"Cholecalciferol (VD3) Attenuates L-DOPA-Induced Dyskinesia in Parkinsonian Mice Via Modulation of Microglia and Oxido-Inflammatory Mechanisms.","authors":"Adedamola Bayo-Olugbami, AbdulRazaq Bidemi Nafiu, Abdulbasit Amin, Olalekan Michael Ogundele, Charles C Lee, Bamidele Victor Owoyele","doi":"10.54548/njps.v37i2.3","DOIUrl":"10.54548/njps.v37i2.3","url":null,"abstract":"<p><p>L-DOPA, the gold standard for managing Parkinson's disease (PD) is fraught by motor fluctuations termed L-Dopa-Induced Dyskinesia (LID). LID has very few therapeutic options. Hence, the need for preclinical screening of new interventions. Cholecalciferol (VD3) treatment reportedly improves motor deficit in experimental Parkinsonism. Therefore, the novel anti-dyskinetic effect of VD3 and its underlying mechanisms in LID was investigated. Dyskinesia was induced by chronic L-DOPA administration in parkinsonian (6-OHDA- lesioned) mice. The experimental groups: Control, Dyskinesia, Dyskinesia/VD3, and Dyskinesia/Amantadine were challenged with L-DOPA to determine the abnormal involuntary movements (AIMs) score during 14 days of VD3 (30 mg/kg) or Amantadine (40 mg/kg) treatment. Behavioral Axial, Limb & Orolingual (ALO) AIMs were scored for 1 min at every 20 mins interval, over a duration of 100 mins on days 1,3,7,11 and 14. Using western blot, striatum was assessed for expression of dopamine metabolic enzymes: Tyrosine Hydroxylase (TH) and Monoamine Oxidase-B (MAO-B); CD11b, BAX, P47phox, and IL-1β. Cholecalciferol significantly attenuated AIMs only on days 11 & 14 with maximal reduction of 32.7%. Expression of TH and MAO-B was not altered in VD3 compared with dyskinetic mice. VD3 significantly inhibited oxidative stress (P47phox), apoptosis (BAX), inflammation (IL-1β) and microglial activation (CD11b). VD3 showed anti-dyskinetic effects behaviorally by attenuating abnormal involuntary movements, modulation of striatal oxidative stress, microglial responses, inflammation, and apoptotic signaling; without affecting dopamine metabolic enzymes. Its use in the management of dyskinesia is promising. More studies are required to further evaluate these findings. Keywords: Cholecalciferol; L-DOPA-Induced Dyskinesia; Parkinson's Disease; Microglial; Oxidative stress; Inflammation.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":" ","pages":"175-183"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10800002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49424307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Academic Staff Strengths of Postgraduate Training Programs in Pharmacology in the USA Pre- and during Covid-19 Pandemic - A Cue for Capacity Building in Pharmacological and other Programs in Nigerian Universities. 新冠肺炎大流行前和期间美国药理学研究生培训项目的学术人员优势——尼日利亚大学药理学和其他项目能力建设的启示
Nigerian Journal of Physiological Sciences Pub Date : 2022-12-31 DOI: 10.54548/njps.v37i2.14
Theresa John, Bode Ekerin
{"title":"Academic Staff Strengths of Postgraduate Training Programs in Pharmacology in the USA Pre- and during Covid-19 Pandemic - A Cue for Capacity Building in Pharmacological and other Programs in Nigerian Universities.","authors":"Theresa John, Bode Ekerin","doi":"10.54548/njps.v37i2.14","DOIUrl":"10.54548/njps.v37i2.14","url":null,"abstract":"<p><p>Structure and function go hand in hand for program success. For the training of pharmacologists, this is also true. A component of structure is academic staff strength. We wanted to know the total academic staff strength of programs and departments in American universities that run training programs in pharmacology at post graduate level in order to inform development in Nigerian and other African academia. Through departmental and program webpages, we mined data on academic staff of departments running graduate programs leading to PhD Pharmacology in the USA. All the data were mined within 96 hours of starting the investigation in 2016 and again in 2021 and were studied using descriptive statistics. There were a total of 25 such programs listed by the American Society for Pharmacology and Experimental Therapeutics (ASPET) website. From the descriptive statistics, the programs were not identical and varied in their staff compositions by numbers. A total of 1,993 academic staff members in 2016 and 2,042 academic staff members in 2021 were serving the 25 graduate pharmacology programs collectively.  Notably more than 25% and less than 50% of the three categories of professorial cadre were non-pharmacology PhD holders. From the composite data of 2016 and 2021, mean staff per program were: 23 professors, 13 associate professors, and 15 assistant professors per program with 2 each of adjunct staff in the categories of professor, associate professors, and assistant professor.  Also composite average per program were 5 joint staff, 3 postdoctoral fellows, 4 emeritus professors, and 11 various researchers. A pattern of top heavy majority of professors was derived from both the 2016 and 2021 investigations. Post docs, miscellaneous researchers, joint staff, and emeritus professors formed a dynamic pool. In totality, staffing of the 25 graduate pharmacology programs in the USA in 2016 and in 2021 were top - heavy experience and expert laden with the professorial cadre, diversified, and depicted dynamism during COVID-19 pandemic. KEY WORDS: academic staff, staff strength, USA universities, Nigerian universities, pharmacology, PhD program.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":" ","pages":"261-270"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45019756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative influence of kolaviron and coenzyme Q10 on complex I activity, glutamate clearance, 3,4-dihydroxyphenethylamine metabolism, and redox stress in rotenone-induced neurotoxicity. 科拉韦隆和辅酶Q10对鱼藤酮神经毒性中复合物I活性、谷氨酸清除、3,4-二羟基苯乙胺代谢和氧化还原应激的比较影响
Nigerian Journal of Physiological Sciences Pub Date : 2022-12-31 DOI: 10.54548/njps.v37i2.2
A C Akinmoladun, Ibrahim Saliu, Olaoluwa Abilogun, Omotola Helen Ajibola, Zainab Abiola Amoo, Olubukola Benedicta Ojo, Ebenezer O Farombi, Mary Tolulope Olaleye
{"title":"Comparative influence of kolaviron and coenzyme Q10 on complex I activity, glutamate clearance, 3,4-dihydroxyphenethylamine metabolism, and redox stress in rotenone-induced neurotoxicity.","authors":"A C Akinmoladun, Ibrahim Saliu, Olaoluwa Abilogun, Omotola Helen Ajibola, Zainab Abiola Amoo, Olubukola Benedicta Ojo, Ebenezer O Farombi, Mary Tolulope Olaleye","doi":"10.54548/njps.v37i2.2","DOIUrl":"10.54548/njps.v37i2.2","url":null,"abstract":"<p><p>3,4-dihydroxyphenethylamine (dopamine) depletion, inhibition of complex I activity, oxidative stress, and glutamate excitotoxicity are cardinal biochemical features of neurotoxicity induced by systemic unilateral infusion of rotenone. Kolaviron (KV), a biflavonoid from Garcinia kola seeds, has been proven to have pharmacological effects against neurotoxicity. Coenzyme Q10 plays an essential role in mitochondrial oxidative phosphorylation and as an antioxidant. This study examined the comparative influence of kolaviron and coenzyme Q10 on complex I activity, dopamine metabolism, glutamate clearance, and redox stress in rotenone-induced neurotoxicity in the cortex, hippocampus, and striatum of the brain of rats. Adult Male Wistar rats were pretreated with 200 mg/kg KV or 100 mg/kg coenzyme Q10 for 7 days followed by administration of a progressive six doses of 1.5 mg/kg rotenone within the next 48 h after which the animals were euthanized and the brain excised. On the cortical, hippocampal, and striatal regions of the brain, complex I activity, dopamine metabolism, oxidative stress markers, as well as glutamate metabolism were carried out and analyzed. In all brain regions examined, KV and coenzyme Q10 pretreatment modulated complex I activity, ameliorated redox imbalance, and enhanced dopamine metabolism via increasing the activity of tyrosine hydroxylase and decreasing monoamine oxidase activity. KV facilitated glutamate clearance through augmentation of glutamate dehydrogenase and glutamine synthetase activities.  The activity of KV was comparable to that of the mitochondrial membrane antioxidant compound, coenzyme Q10, this indicates that KV is a promising therapeutic agent in the treatment of Parkinson's disease and its activity compares well with coenzyme Q10.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":"1 1","pages":"165-173"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41851446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Awareness and Attitudes of Secondary School Students about Physiology Discipline in Southwest Region, Nigeria. 尼日利亚西南地区中学生对生理学科的认识和态度
Nigerian Journal of Physiological Sciences Pub Date : 2022-12-31 DOI: 10.54548/njps.v37i2.13
Oluwaseun Samuel Faborode, Issa Olakunle Yusuf, Damilare Hakeem Adeyemi, Olaolu Joseph Odewole, Joy Adebowale Ajisafe, Ayobami Dare, Olugbemi Temitope Olaniyan, Samuel Adetunji Onasanwo
{"title":"Awareness and Attitudes of Secondary School Students about Physiology Discipline in Southwest Region, Nigeria.","authors":"Oluwaseun Samuel Faborode, Issa Olakunle Yusuf, Damilare Hakeem Adeyemi, Olaolu Joseph Odewole, Joy Adebowale Ajisafe, Ayobami Dare, Olugbemi Temitope Olaniyan, Samuel Adetunji Onasanwo","doi":"10.54548/njps.v37i2.13","DOIUrl":"10.54548/njps.v37i2.13","url":null,"abstract":"<p><p>Physiology remains one of the core disciplines on which all biological and medical sciences were founded. In Nigeria, it is known that most students study Physiology at the undergraduate level by chance and not by choice and end up performing poorly, which could be mainly due to low awareness and knowledge of the discipline, its opportunities, and prospects. Therefore, this study investigated the awareness, attitude, and knowledge about physiology among senior secondary school students in Southwest Nigeria. A cross-sectional of 544 students in science-based senior secondary schools in south-west Nigeria were sampled. Our results showed a high level of awareness, with television being the dominant medium of information. However, knowledge of Physiology was low, while most of the students also showed interest in knowing more about it. Although gender does not seem to influence the level of knowledge, females had a better attitude towards learning about physiology. Similarly, residence did not affect attitude, howbeit associated with the level of knowledge. In conclusion, the high awareness and low knowledge observed in this study would give insights to educate students at the early stages of education about the opportunity and prospects of Physiology and other science-related disciplines.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":" ","pages":"255-260"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45578480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Haematoprotective and Hepatoprotective Effects of Methanolic Leaf Extract of Parquetina nigrescens on Arsenic trioxide-induced Toxicity in Male Wistar Rats. 三氧化二砷对大鼠血液学和生化的影响。
Nigerian Journal of Physiological Sciences Pub Date : 2022-12-31 DOI: 10.54548/njps.v37i2.11
Oore-Oluwapo Daramola, Wahab Adekunle Oyeyemi Oyeyemi, Adeniran Oluwadamilare Akinola Akinola, Yinusa Raji Raji
{"title":"Haematoprotective and Hepatoprotective Effects of Methanolic Leaf Extract of Parquetina nigrescens on Arsenic trioxide-induced Toxicity in Male Wistar Rats.","authors":"Oore-Oluwapo Daramola, Wahab Adekunle Oyeyemi Oyeyemi, Adeniran Oluwadamilare Akinola Akinola, Yinusa Raji Raji","doi":"10.54548/njps.v37i2.11","DOIUrl":"10.54548/njps.v37i2.11","url":null,"abstract":"<p><p>This study was designed to investigate the phytochemical composition and protective effects of methanol extract of Parquetina nigrescens leaf (MEPL) in male Wistar rats. Phytochemical screening, in vitro antioxidant assay, gas chromatography-mass spectrometry (GC-MS) and LD50 were determined. Forty male Wistar rats were grouped into eight and orally treated for 54 days as follows: Group 1 (10% tween 80), Group 2 (3 mg/kg As2O3) Groups 3, 4 and 5 (250, 500 and 1000 mg/kg MEPL) and groups 6, 7 and 8, (250 mg/kg+As2O3, 500 mg/kg+As2O3 and 1000 mg/kg+As2O3). The animals were sacrificed on day 55 under anaesthesia. Blood was collected by cardiac puncture for heamatological studies. Liver concentrations of malondialdehyde (MDA), superoxide dismutase (SOD), aspartate aminotransferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP) activities were determined spectrophotometrically. Liver histology was also assessed. Flavonoids, tannin, alkaloids, saponin, and anthraquinone were present in MEPL, also, MEPL scavenged 2,2 diphenyl-1-picrylhydrazyl hydrate (DPPH) and Azino-bis-3-ethylbenzthiazoline-6-sulphonic acid radical (ABTS+). The IC50 of MEPL required to chelate metal was also low. The GC-MS revealed the presence of 24 essential oil. The LD50 was > 5000 mg/kg. Packed cell volume and red blood cell count were significantly reduced in 1000 mg/kg MEPL group, white blood cell count and SOD activity reduced (P<0.05) in 3 mg/kg As2O3 when compared with control but increased in groups co-treated with As2O3 and 250, 500 or 1000 mg/kg + As2O3. MDA concentration, AST, ALT and ALP activities increased significantly in 3 mg/kg As2O3 group but decreased (P<0.05) in groups co-treated with As2O3 and 250, 500 or 1000 mg/kg. The methanol extract of Parquetina nigrescens leaf in male Wistar rats has antioxidant, hepatoprotective and white blood cell protective effects.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":" ","pages":"235-246"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45902886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antioxidant and Chemopreventive Effects of Azadirachta indica on Lead Acetate-induced Hepatotoxicity in Male Wistar Rats. Azadirachta indica 对雄性 Wistar 大鼠因醋酸铅引起的肝毒性的抗氧化和化学预防作用
Ebunoluwa Asenuga, T O Ajibade, Abiola Adejumobi, Bukola Alaba, Williams Nabofa, Ademola Oyagbemi, Temidayo Omobowale, Joseph Badejo, Olajide Akinboye
{"title":"Antioxidant and Chemopreventive Effects of Azadirachta indica on Lead Acetate-induced Hepatotoxicity in Male Wistar Rats.","authors":"Ebunoluwa Asenuga, T O Ajibade, Abiola Adejumobi, Bukola Alaba, Williams Nabofa, Ademola Oyagbemi, Temidayo Omobowale, Joseph Badejo, Olajide Akinboye","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Toxic metals such as lead (Pb) cause severe liver damage in humans and animals, with oxidative stress prominently implicated in the pathogenesis of lead acetate‑induced liver injury. Azadirachta indica is hepatoprotective due to its antioxidative effect. This study investigated the antioxidative role of A. indica (AI) and its chemopreventive effect on lead acetate (LA)-induced hepatocellular dysfunction with seventy adult male rats classified into group A- Control (distilled water), group B 0.1% LA only, group C and D- 0.1% LA + 100 mg/kg and 0.1% LA + 200 mg/kg AI respectively, group E- 0.2% LA, group F and G- 0.2% LA + 100 mg/kg and 0.2% LA + 200 mg/kg AI. Oxidative stress markers (MDA and H2O2), antioxidant parameters (GSH, SOD, CAT, GPx, GST), inflammatory markers (MPO and NO), alanine aminotransferase (ALT) and histopathological studies of the liver were evaluated. The results showed that LA administration caused a decrease in GSH, GPx, and GST while AI co-administration increased the activities of the antioxidants. Moreover, LA administration increased MPO, NO, MDA, and H2O2 levels whereas AI significantly reduced (P<0.05) these parameters. Histopathological examination revealed necrosis and mild infiltration by inflammatory cells in LA administered rats, whereas these lesions were absent in AI administered rats. In conclusion, A. indica demonstrates a protective role in lead acetate-induced hepatotoxicity, mainly via oxidative stress inhibition.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":"37 2","pages":"219-224"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139503037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neuroprotective Effects of Virgin Coconut Oil Supplemented Diet against Sodium Benzoate-induced Acetylcholinesterase Dysfunction and Cognitive Impairment: Role of Nrf2/NfKb Signaling Pathway. 初椰油补充饮食对苯甲酸钠诱导的乙酰胆碱酯酶功能障碍和认知障碍的神经保护作用:Nrf2/NfKb信号通路的作用
Nigerian Journal of Physiological Sciences Pub Date : 2022-12-31 DOI: 10.54548/njps.v37i2.10
Abraham Asuku
{"title":"Neuroprotective Effects of Virgin Coconut Oil Supplemented Diet against Sodium Benzoate-induced Acetylcholinesterase Dysfunction and Cognitive Impairment: Role of Nrf2/NfKb Signaling Pathway.","authors":"Abraham Asuku","doi":"10.54548/njps.v37i2.10","DOIUrl":"10.54548/njps.v37i2.10","url":null,"abstract":"<p><p>The effect of virgin coconut oil (VCO) supplemented diet on sodium benzoate (SB) - induced neurotoxicity in male Wistar rats was investigated. Twenty (20) male Wistar rats weighing 160-180g were divided into four (4) groups: Control which received 1ml of normal saline, SB-treated; received 200 mg/kg b.w, SB + Low Dose VCO-treated (SB + 5% VCO mixed with 95g of rat chow), and SB + High Dose VCO-treated (SB+ 15% VCO mixed with 85g of rat chow). The brain was processed for NRF-2, NF-kB, and acetylcholine esterase (AchE) gene expression levels. Also, the blood sample was processed for assessment of superoxide dismutase (SOD), catalase (CAT), and IL1B levels. One-way ANOVA and Tukey post hoc tests were used to analyze data. SB-treated rats with no intervention showed anxiety-like behavior and impaired memory as depicted by a significant (p<0.0001) increase in anxiety index, increase in brain NF-KB, increase in serum IL1B and increase in AchE gene expression level, reduction in the recognition ratio, decreased spontaneous alternation performance, decreased CAT and SOD levels and decreased NRF-2 expression level when compared to other groups (especially control and SB + 5% VCO). VCO supplemented diet (both 5% and 15%) significantly (p<0.0001) increased the CAT and SOD levels, increased the NRF-2 gene expression level, and significantly (p <0.0001) decreased the IL1-B level. Moreover, 5% VCO significantly (p<0.0001) decreased the anxiety index, decreased AchE and NFkB gene expression levels, increased spontaneous alternation performance, and increased recognition ratio compared to 15% VCO. VCO shows a neuroprotective effect in attenuating cognitive impairment and anxiety-like behavior in SB-induced model by modulating oxidative stress and inflammatory pathways, and also enhancing cholinergic neurotransmission. Keywords: Virgin coconut oil; sodium benzoate; acetylcholinesterase; catalase; superoxide dismutase; oxidative stress.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":" ","pages":"225-233"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42854066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antihyperlipidemic property of Murraya koenigii ethanolic leaf extract on lipid profile of rats fed with cholesterol-enriched diets. 葛乙醇叶提取物对高胆固醇饮食大鼠血脂的影响
Nigerian Journal of Physiological Sciences Pub Date : 2022-12-31 DOI: 10.54548/njps.v37i2.8
Azubuike Raphael Nwaji
{"title":"Antihyperlipidemic property of Murraya koenigii ethanolic leaf extract on lipid profile of rats fed with cholesterol-enriched diets.","authors":"Azubuike Raphael Nwaji","doi":"10.54548/njps.v37i2.8","DOIUrl":"10.54548/njps.v37i2.8","url":null,"abstract":"<p><p>This study was designed to investigate the effect of orally administered ethanolic leaf extract of Murraya koenigii on lipid profile of rats fed with cholesterol-enriched diets for 14 days. The study was carried out on 20 male wistar rats divided into 4 groups (n=5). All animals were given ad libitum access to drinking water. Group A was the control group and received normal rat chow, group B received cholesterol-enriched diet (CED); while group C and D received cholesterol-enriched diet and were treated with 200mg and 400mg/kg body weight of ethanolic extract of Murraya koenigii leaf respectively. Blood samples were collected by cardiac puncture on the last day of the experiment for biochemical analysis. Lipid profile was determined by Colorimetric method. Data were expressed as mean ± SEM. Differences in means were compared using ANOVA and Students T-test, P-values ≤ 0.05 were taken as statistically significant. Results showed a significant increase (p ≤ 0.05) in Total cholesterol (TC) level (117.4 ± 4.6) and low density lipoprotein cholesterol (LDL-c) level (50.1 ± 4.7) of group B rats when compared to other groups. The MK (400 mg/kg) treated rats, showed a significant reduction in TC and LDL-c levels, and a significant increase in HDL-c level when compared to CED group. However, there were no significant changes in all the lipid parameters of the treated groups when compared to the control. This study shows that cholesterol-enriched diet causes dyslipidemia and Murraya koenigii exhibited significant hypolipidemic effect on serum TC and LDL-c and increases HDL-c. Hence, Murraya koenigii exhibit antihyperlipidemic properties. Keywords: Murraya Koenigii, hyperlipidemia, Lipid profile, Cholesterol-enriched diet.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":" ","pages":"215-218"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49545840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Consumption of Aqueous Leaf Extract of Jatropha tanjorensis Improves Fertility Potential and Gestational Outcome in Virgin Female Wistar Rats. 食用麻疯树水叶提取物可提高处女雌性 Wistar 大鼠的生育能力和妊娠结果。
Imoh Ukoh, Bisong Sunday, Atim Antai, Samuel Arikpo, Henrietta Nsa, Izuka David
{"title":"Consumption of Aqueous Leaf Extract of Jatropha tanjorensis Improves Fertility Potential and Gestational Outcome in Virgin Female Wistar Rats.","authors":"Imoh Ukoh, Bisong Sunday, Atim Antai, Samuel Arikpo, Henrietta Nsa, Izuka David","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In spite of the overwhelming patronage of the plant Jatropha tanjorensis (J.T) popularly called \"Hospital Too Far\" by pregnant and women of reproductive age for its supposed reproductive benefits, the scientific evidence are hardly known. This study thus sought to examine the effect of consumption of aqueous leaf of J.T extract on female fertility potential and gestational outcome using forty (40) virgin female Wistar rats weighing 120-150g. The extract LD50 was >5000 mg/kg. The female rats were divided into unmated and mated groups (n=10 rats per subgroups). Group A (unmated group) consisted of control which received 0.2mL of normal saline (vehicle) and J.T-treated which received 500mg/kg (orally) once daily for 28 days. For Group B (mated group), the treated dams received 500 mg/kg (orally) once daily prior to mating (2 weeks), during mating (1 week) and throughout gestation, while the control received 0.5ml of physiological saline orally during the same period. All animals had free access to food and water. For the unmated group, after 28 days of treatment samples were collected for hormonal assay. J.T increased follicle stimulating hormone (FSH) and estrogen. For subgroup B, the extract was assessed for it reproductive and gestational performance, as well as pregnancy outcome. Nine (9) J.T-treated rats against seven (7) of control got pregnant. J.T increased mean weight gain, food and water intake. The Birth weight, body length and tail length of pups whose mothers were treated with the aqueous leaf extract of J.T increased significantly. Intake of J.T was effective in boosting female reproductive health, pregnancy health and outcome.</p>","PeriodicalId":35043,"journal":{"name":"Nigerian Journal of Physiological Sciences","volume":"37 2","pages":"247-253"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139503040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信