Plant Cell Biotechnology and Molecular Biology最新文献

{"title":"EFFICIENT NUTRIENT MANAGEMENT IN FIELD CROPS FOR FOOD AND ENVIRONMENTAL SAFETY","authors":"M. Bashir, M. A. Bhat, Shailja Sharma, Navjot Rana, Suhail Fayaz, S. Iqbal, Razia Gull, .. RAHEEBA-TUN-NISA, Wani Barkat UL Islam, .. Dushyant, Dhamni Patyal","doi":"10.56557/pcbmb/2022/v23i39-408030","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i39-408030","url":null,"abstract":"The current agricultural producers are under immense strain to supply rising food demand due to rising population and dwindling land and other resources. Several critical inputs, such as fertilizers and other chemicals, are exploited to address this challenge, creating environmental damage. Soil health has deteriorated, land degradation has occurred, and major environmental challenges have arisen as a result of intensive agricultural production without respect for environmental sustainability. As a result, future efforts to feed the world's growing population should prioritize increasing agricultural output while remaining ecologically responsible. In this sense, novel approaches are required, as current policies are incapable of addressing these issues. The notion of efficient nutrient management, which includes a variety of strategies for agricultural sustainability, as well as numerous soil and crop management strategies (SCMS) meant to maximise crop output under environmentally sustainable conditions, is addressed. Balanced fertilization, minimized use of chemicals such as fertilizers and pesticides, and improved crop input utilization efficiency could help reduce greenhouse gas emissions while simultaneously saving the environment. Sustainable agriculture holds promising applications for humanity and the environment, and it can be realised if industrialized and developing countries collaborate to establish a \"shared vision\" that produces more food with less ecological consequences.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48884871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TRIPLE TEST CROSS ANALYSIS FOR DETERMINING GENE ACTION, GENETIC CORRELATIONS AND PREDICTION FOR YIELD AND YIELD COMPONENTS IN BREAD WHEAT","authors":"A. Kandil, M. Abdel-Moneam, Hashad Soad, S. G. Hasnaa","doi":"10.56557/pcbmb/2022/v23i39-408013","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i39-408013","url":null,"abstract":"In two crosses of bread wheat (Gemmeiza11 x Misr1 and Gemmeiza12 x Misr2) to investigate gene action, genetic correlations and predicating new recombinant lines for days to heading, plant height, flag leaf area, number of spikes/plant, number of grains/spike, 1000-grain weight, spike grain weight and grain yield /plant, triple test cross was used. The starting materials for triple test cross was the F1 and their grand parents (P1 and P2) for the two studied wheat crosses. The first cross Gemmeiza 11 x Misr1 and the second cross was Gemmeiza 12x Misr2. The F1’s were selfed to produce F2’s grains. In order to create F1 and F2 plants, the obtained materials (F1 and F2 grain genotypes) and the parental genotypes were seeded together. Thirty individual F2 plants were randomly labeled from each cross and crossed back to their grand parents (P1 and P2) and F1 between then to produce three types of families L1 (F2i x P1i), L2 (F2i x P2i) and L3 (F2i x F1i) in each cross. On November 10, 2018, during the winter growing season, the triple test cross families (L1, L2 and L3), along with the parents, F1 and F2 in each cross, were seeded. Results showed that in the genetic system for the investigated features for two crosses, epistatic gene effects were significant. The types of epistasis (additive x additive, additive x dominance and dominance x dominance) were significant for all studied characters. For the majority of traits in both crossings, both additive and dominant genetic components were significant and involved in the genetic system. The average level of dominance for spikes/plant in cross 1 and spike grain weight in cross -2 was overdominance, while for the remaining characters, additive gene effects were more pronounced. The F value was positive and significant for 1000-grain weight in two crosses, suggesting that dominant genes controlling these characters were unidirectional. The highest proportion of inbreds excepted to outperform parental rang in cross 1 for days to heading and flag leaf area. In both crosses, grain yield per plant was positively and significantly correlated with number of spikes per plant, number of grains per spike, and 1000-grain weight, according to epistasis, additive, and dominance genetic correlations.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45749901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RESPONSE OF Jatropha curcas PLANTS TO EM WHEN GROWN IN SAND SOIL AMENDED WITH ORGANIC MATTER","authors":"R. Said, H. Ghazzawy","doi":"10.56557/pcbmb/2022/v23i39-407996","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i39-407996","url":null,"abstract":"This study consummated in the open field at the nursery of Hort. Res. Inst., A.R.C., Giza, Egypt during 2020 and 2021 seasons to explore the response of Jatropha curcas  plants to EM (effective microorganisms) which was applied as soil drench at 3 ml/L, 5 times with one month interval. Plants cultured in 25-cm-diameter plastic pots filled with sand only as control (T1), sand + plant compost 10 % mixture (T2), T2 + EM (T3), sand + plant compost 20 % mixture (T4), T4 + EM (T5), sand + plant compost 30 % mixture (T6) and T6+ EM (T7). The obtained results showed that means of the different growth parameters ;expressed as:  plant height, number of leaves / plant , leaf area , root length , as well as fresh and dry weights of stem, leaves and roots were ascending increased as the ratio of compost was increased in the soil especially with EM treatments compared to means of control in most cases of the two seasons, otherwise stem diameter trait, which progressively increased with the middle ratio of compost (20 %) and compost (20 %) + EM followed by the highest one (30 %) and (30%) + EM reached to the lowest values of compost (10 %) and (10 %) + EM over control in the first and second season. A similar trend was also obtained regarding the leaf content of chlorophyll a, b, carotenoids beside the percentages of N and total carbohydrates which were recorded the highest values with sand + compost (30%) subsequent sand + compost (20%) and sand + compost (10%) individually or with EM. Whereas, the leaf contents of P% and K% significantly increased in sand + compost (20%), (30 %) and then (10%) whether with EM or without. Hence, it can be recommended to drench EM (3 ml/l) in sand soil mixed with plant compost (30 %) in which  J. curcas plants are grown to keep good growth and the best quality plant.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45031444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVALUATION OF PROXIMATE COMPOSITIONS OF INDIGENOUS AND UNDERUTILIZED GREEN LEAFY VEGETABLES OF NORTH GUJARAT, INDIA","authors":"P. K. Patel, J. Dhaduk, S. S. Patil","doi":"10.56557/pcbmb/2022/v23i39-407993","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i39-407993","url":null,"abstract":"Human needs wide range of nutrients for growth, development and to lead an active and healthy life. Green leafy vegetables are rich sources of carotenoids (Vitamin 'A') as well as appreciable amounts of minerals such as calcium, phosphorus, potassium, sodium, iron, zinc, manganese and copper. In view of less utilization of some of tradition leafy vegetables by people of Banaskantha district and paucity of scientific information on nutritive value, it is proposed to assess nutritive value of less familiar, six underutilised green leafy vegetables viz., dodi, poi, kanjaro, khatedo, radish and sarsiya for proximate composition. Healthy, six green leaves were randomly collected in fresh conditions from farmer’s fields of surrounding villages and subjected to analysis of different nutrients. At the end of the study it was found that Dodi (Leptadenia reticulate) and kanjaro (Digera arvensis) have high percentage of crude protein, ash and crude fibre as compared to other underutilized vegetables and hence can be used routinely as a part of diet to fulfil the nutrient requirement of  peoples of Banaskantha region.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41264754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro CYTOTOXICITY AND APOPTOTIC INDUCING EFFECTS OF Dimera acutipus ON MCF-7 BREAST CANCER CELLS","authors":"S. Sivagnanam, Sajusha Duguluri","doi":"10.56557/pcbmb/2022/v23i39-407956","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i39-407956","url":null,"abstract":"Cancer is a complex multistage diseases process. Surgery and radio therapy are failing to cure certain kind of malignancies chiefly because of the tumour is already disseminated for this reason the chemotherapeutic agents are sought. Therefore, there is need to find out a new class of anti-cancer agents with no or less toxic from medicinal herbal plant sources. Hence it is of interest to investigate the in-vitro analysis cytotoxicity and apoptotic inducing efficacy of the plant extract studies were undertaken. The in-vitro analysis of cytotoxicity of the chloroform extract of Dimera acutipus were analysed by using the MTT assay and the apoptotic analysis was performed by the flow cytometric analysis. The present study is to explore the cytotoxicity as well as the apoptotic effects of the chloroform extract of aerial parts of Dimera acutipus in normal Vero cell lines and human breast cancer cell lines. The main aim of this study is to evaluate the cytotoxicity of the plant extract in Vero and MCF-7 cell lines was undertaken. Our results indicate that non cytotoxic nature of the chloroform extract of Dimera acutipus in appropriate cell lines. In conclusion the anti-apoptotic analysis clearly indicates the chloroform extract of Dimera acutipus has the ability of cell cycle arresting potential in conclusion the present investigation clearly envisages a plant extract could be a strong anticancer agent.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45935729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ROLE OF AMINO ACIDS IN IMPROVING GROWTH, YIELD AND PHYSIOLOGICAL PARAMETERS OF SOME EGYPTIAN CLOVER CULTIVARS","authors":"A. Badawy, S. El-Nahrawy, R. El-Mantawy","doi":"10.56557/pcbmb/2022/v23i39-407955","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i39-407955","url":null,"abstract":"The lack of green forage of good quality is considered the main constraints that contribute to the lack of livestock development. An experiment was performed at Sakha Agricultural Research Station Farm, Kafr El-Sheikh Governorate, Egypt, to study the role of spraying commercial amino acids (g-plus) and pure tryptophan on forage yield and chemical composition of three Egyptian clover cultivars during the two successive winter seasons 2019/2020 and 2020/2021. \u0000 The experiment was laid out in a strip-plot design with three replications. The main plots were occupied by amino acids treatments i.e. spray with water (control), spray with g-plus (5cm/l water) and spray with tryptophan at two concentration (200 and 400 mg/l). While sub-plots contained three cultivars of berseem; Helaly, Serw1 and Giza 6. The results showed that, the maximum total fresh forage yield, total dry forage yield, average of plant height, number of stem per 0.25 m2, fresh leaf/stem percent and dry leaf/stem percent were obtained for variety Helaly spraying by amino acid g-plus (5cm/l water). Also, the obtained results showed that, foliar spraying of amino acids caused significant increase of most physiological characters in comparison to the control. The highest values of chlorophyll content, relative water content (RWC %), protein % and NPK% were obtained with spraying g-plus. Concerning, the cultivars effects showed a wide range of variation in their productivity where, Helaly was the highest values of the most physiological parameters as well as yield and yield components. The interaction effects of foliar spraying of amino acids and cultivars showed that, Helaly treated with g-plus (5cm/l) gave the highest values of growth and physiological parameters.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44872915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro MULTIPLE SHOOT INDUCTION OF Citrus aurantifolia","authors":"Punyakishore Maibam, V. Sahi","doi":"10.56557/pcbmb/2022/v23i39-407942","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i39-407942","url":null,"abstract":"The present study provides an efficient and simple method for the micropropagation of C. aurantifolia which can be effectively used for its mass propagation. Of all the different concentrations used, the maximum number of shoots was initiated in the medium supplemented with 1.5 mg/l of BAP. The medium supplemented with 1.5mg/l BAP and 1.5 mg/l KN was proven to be the best for shoot induction with maximum response of explants (79.57 %) as well as the maximum number of shoots per explant with shoot length. However, there was no significant response from the explant inoculated in the medium supplemented with cytokinin (KN and BAP) in combination with auxin (2, 4-D and NAA). NAA was found to be more effective for root induction than IAA. The regenerated shoots cultured in MS medium supplemented with 1.5 mg/l NAA resulted in maximum response of 83.52%.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42308446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ASCERTAINING THE EFFECT OF BAP (6- BENZYL AMINO PURINE) ON ROSE (Rosa spp) THROUGH MICROPROPAGATION","authors":"Shakira Afnan Mohammad Sadar, Sowmiya Madhaiyan, Sneaha Mahadevan, Shalini Govindaraj, Sindhuja Ravichandran, Sowmiya Jayakumar, Tharshenee Umapathy, Vidhya Lakshmanasamy, Sampath Sarangapani, Ashok Subiramaniyan, Arul Ganesh Thangaraj, V. Muthu, R. Moorthy, Sala Murugan, Shunmugavadivel Thangavel, Sarankumar Chandran","doi":"10.56557/pcbmb/2022/v23i37-387924","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i37-387924","url":null,"abstract":"In this investigation, Rose nodal explants from healthy shoots were grown on a base MS medium supplemented with various BAP concentrations (6-Benzyl Amino Purine). It was determined from the various concentrations that the shoot initiation and development were best at the concentration of 3 mg/lit. From the third day to a one-month interval, the various stages of shoot development were examined. It was revealed that callus initiation begins on day 10, axillary shoot initiation on day 2, and that growth and development begin on day 3 and last for a month, respectively. Additionally, the established healthy shoot culture will be kept up and given root initiation treatment using rooting media. The ideal rooting media concentration will be fixed for better root initiation and development.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45231706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STUDY ON GENETIC VARIATION OF KELEMBAK (Rheum officinale Baill.) USING RAPD MOLECULAR MARKERS IN CENTRAL JAVA, INDONESIA","authors":"Dyah Widodo, Devi SAFRINA, Rohmat Mujahid, Y. Widiyastuti","doi":"10.56557/pcbmb/2022/v23i37-387911","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i37-387911","url":null,"abstract":"Kelembak (Rheum officinale Baill.) is native to mainland Central China and is reported as an endangered medicinal plant. This plant in Indonesia is distributed in mountainous areas; the center of rhubarb cultivation is in the Dieng Plateau. Mainly, this plant is grown for the roots as herbal medicine material (kelembak jamu) and in the industry for cigarette ingredients (kelembak Jawa). This study aimed to assess the genetic diversity of nine accessions of R. officinale. Six selected RAPD primers were used to amplify the samples and produced 33 DNA fragments ranging from 270-2,710 bp. The genetic similarity ranged from 74.58% to 100% using the Dice similarity index. The dendrogram divided R. officinale into two main clusters separated between kelembak jamu and kelembak jawa. Cluster analysis revealed that Mangli accession (kelembak jamu) had a closer similarity to kelembak Jawa. The narrow genetic diversity of kelembak is essential information for future conservation and management programs in the cultivation of this species.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49358486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INTERACTIVE EFFECTS OF GENOTYPE AND WATER DEFICIT ON YIELD AND QUALITY TRAITS OF MAIZE INBRED LINES AND F1 DIALLEL CROSSES","authors":"A. Al-Naggar, A. Soliman, Elham Mohamed Hassan Mohamed","doi":"10.56557/pcbmb/2022/v23i37-387899","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i37-387899","url":null,"abstract":"Maize (Zea mays L.) is vulnerable to drought stress at flowering stage, which has a detrimental impact on yield characteristics. Drought tolerance is genotype dependent.  The present study was conducted to investigate the effects of Water Deficit Stress (WDS) at flowering stage, genotype and their interaction on some agronomic, physiologic, grain yield and quality traits of 8 maize inbred lines and their 28 diallel crosses and also attempt to identify the maize genotypes tolerant to WDS conditions. The parents and F1 crosses were evaluated in two seasons. A split plot design with three replications was used, where main plots were allotted to two irrigation treatments, i.e., Well Watering (WW) by giving all recommended irrigations and WDS by withholding the 4th and 5th irrigations, while sub plots were allotted to genotypes. Water deficit stress caused a significant decrease in grain yield/plant (32.3%), grain yield/ha (26.7%), ears/plant (30.6%), rows/ear (10.2%), kernels/row (9.3%), kernels/plant (43.8%), 100-kernel weight (19.9%), plant height (8.5%), ear height (11.9%), and chlorophyll concentration index (17.3%) and a significant increase in anthesis-silking interval (58.82 %), days to anthesis (4.29%), days to silking (6.40%), barren stalks (20.0%), and grain protein content (16.0%). On average, means across F1 crosses were higher than those across inbreds for all studied traits, except for grain protein content and days to 50% silking, where the opposite was true, under both WDS and WW conditions. The rank of inbreds and crosses for studied traits under WDS was changed from that under WW conditions. The reduction in grain yield/ha of inbred lines due to drought (37.36%) was generally higher than that in F1 hybrids (25.67%). It was possible to identify some inbreds and hybrids characterized by high yield and high tolerance to WDS conditions, which were recommended for use in breeding programs for improving drought tolerance in maize.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44785651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}