Plant Cell Biotechnology and Molecular Biology最新文献

{"title":"CLONAL VARIATION OF GROWING AND MAHOGANY (Swietenia macrophylla King) CLONE SELECTION IN AN ASEXUAL SEED ORCHARD","authors":"José Vidal Cob Uicab, B. Santiago, Gilbert José Herrera Cool, Ángel Javier Cammal Collí","doi":"10.56557/pcbmb/2022/v23i37-387894","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i37-387894","url":null,"abstract":"Swietenia macrophylla King. (Mahogany) is a tropical tree of timber use which is classified as one of the principal economic and forest important native species in Latin-American. There are few works about germplasm of superior genetically trees. This germplasm source is very important to establish commercial forestry plantations. For this reason, the objective of this work was to select superior clones from an asexual seed orchard which was established in September 2014. The orchard was established under a randomized complete block experimental design in a royal frame of distance 4x4 m, in which 50 clones (ortet) with 49 replicates (ramet) were cultured obtaining 2,450 ramets in total. Variables evaluated were total height, clean stem height, normal diameter and crown diameter. It was carried out a principal components analysis and an analysis of variance (ANOVA). Furthermore, a test of comparison of means using the Tukey test at 5% of probability level (p≤0.05) and a conglomerate analysis was carried out. From 50 clones, it was identified 16 genetically superior materials: 1-CaLK149, 28-CaNB23; 23-CaNB48; 7-CaLK147; 20-CaNB14; 14-CaNB26; 25-CaNP132; 3-CaNP143; 9-CaB102; 24-CaLK147; 18-CaNB24; 19-CaNB19; 11-CaNB26; 13-CaNB21; 29-CaNB19; 21-CaNB26, these materials showed an average of 8.6±0.3 m of total height, 2.4±0.1 m of clean trunk height, 0.13±0.0 m of normal diameter and 4.3±0.2 m of tree crown diameter. Those clone groups identified as superior trees were recommended to continue in a forest breeding program.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43036553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CHITOSAN AND AMINO ACIDS-COATED NANOPARTICLES POSITIVELY ALTERED ATROPINE ACCUMULATION, THE GENE EXPRESSION OF Tropinone reductase 1 AND THE YIELD OF Datura innoxia MILL","authors":"Asmaa A. Mahdi, M. Suliman, K. Farroh, O. Ghaly, E. Hamed, H. Ibrahim","doi":"10.56557/pcbmb/2022/v23i37-387895","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i37-387895","url":null,"abstract":"Two consecutive seasons (2020 and 2021) were held for cultivating Datura innoxia Mill as a medicinal plant for increasing the production of seeds and active ingredients as a native goal for our economy by using several aspects of chitosan (bulky and nanoparticles) as a biocompatible and biodegradable substance because of its crustacean origin and amino acids (individually and loaded onto chitosan nanoparticles). Data showed that formulated chitosan nanoparticles 100 mg/L loaded with methionine 40 mg/L gave an excellent record of fruit number per plant and seed weight per plant, and the highest protein content combined with the lowest range of the toxic compound malondialdehyde. Additionally, applying chitosan nanoparticles 50 mg/L coated with methionine 40 mg/L to D. innoxia plants resulted in the highest accumulation of atropine alkaloid and several polypeptides in the protein electrophoresis (SDS PAGE) when compared with control. Furthermore, in the roots of D. innoxia, where tropane alkaloids are synthesized, this application induced the expression of the tropinone reductase 1 (DiTR1) gene, a key gene in the synthesis of tropane alkaloids. So, the target of using nanotechnology applications (chitosan nanoparticles functionalized with methionine) in agriculture, especially medicinal plants, to increase yield and secondary metabolites has proven helpful, according to our findings. Future biological studies for these treatments are recommended, in order to test their safety on plants and humans.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49131808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IMPROVING DROUGHT STRESS TOLERANCE IN POTATO (Solanum tuberosum L.) USING MAGNETITE AND ZINC OXIDE NANOPARTICLES","authors":"Amira R. Sallam, Asmaa A. Mahdi, K. Farroh","doi":"10.56557/pcbmb/2022/v23i37-387886","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i37-387886","url":null,"abstract":"Potato is a very sensitive to drought stresses, necessitates specific amounts and quality of water. Making substantial changes to increasing tolerances are not easily due to their narrow genetic base. Researchers developed many techniques to reduce the effects of abiotic stress. In current work we studied the effect Nano Particles of Zinc dioxide (ZnO-NPs) and Magnetite (Fe3O4-NPs) at (0.0, 2.5, 5.0 ppm) in elevating the negative effect of drought stress (Sorbitol) at (0.0, 1, 2, 3, 4 Mm) on micropropagation, microtuberization and some biochemical characters, using two potato cultivars (Almond, and Picasso). Maximum mean values were achieved by Almond cultivar and by ZnO-NP in most vegetative, harvesting parameters in vitro. In experiment 1: increases sorbitol levels caused reduction in all parameters inversely, and growth was stopped completely by (0.40) M which considered as a lethal dose to potato explants for both cultivars. In experiment 2: results illustrated that, significant improved due to add nanoparticles of ZnO or Fe3O4 at two concentrations (2.50 and 5.00) ppm on all morphological, harvesting parameters at 0.40 M and 0.3 M of sorbitol. About secondary metabolites: the maximum record of flavonoids (querecetin and kaempferol) and antioxidant capacity was achieved by Almond with sorbitol 0.3M. Almond also recorded the maximum mean of quercetin, kaempferol and, DPPH scavenging activity was decreased in sorbitol 0.3M for both NPs treatments. At 0.4M, secondary enhanced accumulation metabolites and scavenging activity especially at ZnO NPs (5 ppm) and Fe3O4 NPs (2.5 ppm), in quercetin and kaempferol with Fe3O4-NPs (5.0 ppm).","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42264956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CHEMICAL COMPOSITION BY GC-MS, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF PEELS AND LEAVES EXTRACTS FROM Citrus limon, Citrus sinensis AND Citrus reticulata","authors":"Hadjer Boussoussa, Ihcen Khacheba, Sarah Boukhalkhal, Y. Lamri, Ahlam Bouti, Artur M. S. Silva, M. Yousfi","doi":"10.56557/pcbmb/2022/v23i35-367885","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i35-367885","url":null,"abstract":"Citrus family especially lemon, mandarin and orange, have reached important attention due to the presence of several components with different therapeutic properties. The leaves and peels of non-polar extracts from bio-citrus cultivars were investigated for phytochemical analysis, antibacterial and antioxidant capacity. The DPPH (1,1-Diphenyl-2-picrylhydrazyl) activity and phosphommolybdenum antioxidant effect values from leaves and peels ranged from 0 to 12.79% and 0.006 to 0.14 mg/ml AAeq respectively. Results showed significant differences in antioxidant properties of leaves and peels extracts. The leaves of mandarin exhibited a higher antioxidant activity than the other extracts from lemon and orange. Among different components identified from the organic extracts, limonene, myrcene, and benzopyran were major components in all samples.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44917263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TAXONOMIC STUDY OF FRESHWATER ALGAE FROM WATER BODIES IN HALLOKI VILLAGE, LAHORE, PAKISTAN","authors":"Tooba Zia, G. Butt, F. Arif, Hassan Nawaz, Saba Zafar, Amina Fareed","doi":"10.56557/pcbmb/2022/v23i35-367878","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i35-367878","url":null,"abstract":"Halloki village Lahore was explored for freshwater algae and 7 samples were collected from different sites (Freshwater ponds, marshy areas, irrigated channels, watered field, road side streams, etc) during October 2020 to April 2021. Total nineteen genera and thirty four species of algae belonging to three kingdoms; Monera, Protista and Protoctista were recorded for the very first time from Halloki village Lahore, Pakistan, rest of them have previously been reported other sites of Lahore (lil. cit). Kingdom Monera included one phylum Cyanophycota (8.82%) containing 1 class, 1 order, 2 families, 2 genera and 3 species i.e. Oscillatoria irrigua, O. tenuis and Anabaena aequalius. Kingdom Protista was found to be most abundant having three phyla; Volvophycota (29.40%), Euglenophycota (2.94%) and Bacillariophycota (29.41%) containing 4 classes, 6 orders, 11 families, 13 genera and 21 species i.e. Crucigenia quadrata, Closteridium lunula, Palmodictyon viride, P. varium, Cosmarium subcreantum, Closterium lanceolatum, C. acerosum, C. parvulum, C. cornu var. arcum, C. lunula var. lunula f. biconvexum, Euglena polymorpha, Cymbella affinis, Nitzchia vasnii, N. palea, Acanthes trigibba, Navicula disjuncta, N. microspora, N. exigua, Anomoeoneis exilis, Synedra ulna and Melosira varaians. Kingdom Protoctista included one phylum Chlorophycota (29.40%) containing 2 classes Ulvophyceae (11.76%) and Zynemophyceae (17.64%) respectively, 4 orders, 4 families, 4 genera and 10 species i.e. Microspora ameona, M. crassior, M. pachyderma, Ulothrix subconstricta, Oedogonium nodulosum, Spirogyra condensata, S. aplanospora, S. intorta, S. bullata and S. acquinoctialis.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44920289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA BARCODING OF SOME ZYGOPHYLLACEAE SPECIES IN THE EGYPTIAN FLORA","authors":"R. Khalil","doi":"10.56557/pcbmb/2022/v23i35-367864","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i35-367864","url":null,"abstract":"DNA barcoding is gaining a high popularity and due to its simplicity and high accuracy as compared to the complexity associated with morphological identification of taxa. Therefore, we tested the potential of the rbcL, matK and ITS f markers for the identification of wild plants belonging to diverse Zygophyllaceae species from different sites of Egypt. Maximum likelihood tree analysis was performed to evaluate the resolution power of each marker to discriminate species. The analyses of nucleotide sequences of rbcL, matK and ITS revealed that the topology of the rbcL tree was the best resolved which enabled identification of the majority of the species, perfectly. Therefore, we can suggest use of the rbcL region as an additional tool for phylogenetic analysis in Zygophyllaceae species. We recommend this study for any further studies on Zygophyllaceae family and contribution to taxonomical researches by adding a new dimension to the Egyptian flora.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45066040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECT OF FRESHWATER ALGAE ON GROWTH OF Brassica oleracea L.VAR. MADHURI & PELICAN","authors":"Amina Fareed, G. Butt, Syeda Rida Zahra, Tooba Zia, Umal Bashir","doi":"10.56557/pcbmb/2022/v23i35-367859","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i35-367859","url":null,"abstract":"An experiment was conducted in Phycology Lab Department of Botany, Government College University Lahore, to investigate the effect of freshwater algae on the seedling and vegetative growth of Brassica oleracea L. Var. Madhuri and Pelican. Different concentrations of FLF (25%, 50%, 75%, 100% and control) were prepared. The parameters like GP, MGT, GI, GE, and SVI were measured. In in-vitro experiment, the readings like GP. GM, GE, MGT and SVI were maximum at 100% FLF concentrations. The chlorophyll content, photosynthetic efficiency, plant growth rate, nitrogen content, crude protein content and epicotyl length were maximum at 100% FLF concentration for Brassica oleracea L. Var. Madhuri & Pelican. In ex-vitro experiment, 75% FLF concentration proves effective in increasing TSL, RL, SL, and FW as compared to 25%, 50% & 100% FLF concentration and control. In vegetative growth experiment, the parameters like TPH, GP, RL increase with increase in FLF concentrations upto a certain limit. The plants which are given 75% of FLF concentrations show increase in growth as compared to the plants given high (100%) and low (25% & 50%) FLF concentrations and control. The plants treated with 100% FLF concentration has more ionic content. Amount of sodium, potassium and nitrogen present in plants treated with 100% FLF concentration is high as compared to plants treated with 25%, 50% and 75% FLF concentrations and control.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43408971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MOSQUITO LARVICIDAL POTENCY OF SELECTED HALOPHYTE SPECIES AND THEIR MODULATION ON ACETYLCHOLINESTERASE AND GLUTATHIONE S-TRANSFERASE AGAINST DENGUE VECTOR: Aedes aegypti","authors":"Pratik P. Dhavan, .. Ranjana, B. Jadhav","doi":"10.56557/pcbmb/2022/v23i35-367856","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i35-367856","url":null,"abstract":"A mangrove is an ecosystem that represents a natural product reservoir of biotechnological  interest. They have been advocated as environmentally benign alternatives to synthetic pesticides against all vector mosquitos. The purpose of this study was to determine the larvicidal activities of selected mangrove plant extracts against Aedes aegypti mosquito larvae. Methanol extracts of three selected halophytes viz. Lumnitzera racemosa, Bruguiera cylindrica, and Excoecaria agallocha were made, phytochemically investigated and evaluated against A. aegypti early 4th instar larvae. Acetylcholinesterase (AChE) activity and Glutathione S-transferase (GST) activity were evaluated, followed by DNA extraction and fragmentation tests. GC-MS technique was used to determine the presence of bioactive chemicals. Alkaloids, flavonoids, terpenoids, anthocyanin, coumarins, phenolic compounds, and quinones were found in all plants. The best larvicidal activity was recorded in the L. racemosa flower buds (LC50- 0.766 mg/ml) and E. agallocha stem extract (LC50- 0.986 mg/ml).  L. racemosa flower buds and E. agallocha stem exhibited higher inhibition in Acetylcholinesterase (AChE) activity with the rate of 1.39 μmol ACT/min/mg protein and 1.65 μmol ACT/min/mg protein (p < 0.05) in treated larvae. In the case of L. racemosa flower buds, however, enhanced GST activity was detected. Plant extracts were found not to cause any fragmentation activity in the larval DNA. 5 major peaks of chemical compounds were analyzed in L. racemosa and E. agallocha. Because of the possible larvicidal action of selected halophyte extracts, they can be utilized as ecologically friendly mosquitocide for eradicating dengue vector larvae in their early stages.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48200481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MONOCULTURING OF SOME FRESH WATER ALGAE FROM A WATER BODY IN NASIR BAGH ON CHU#10 MEDIUM","authors":"Midhat Mahboob, G. Butt, Amina Fareed, Umal Bashir, Tooba Zia, Aneeza Attique","doi":"10.56557/pcbmb/2022/v23i35-367857","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i35-367857","url":null,"abstract":"Microalgal samples were collected for monoculture of some freshwater algae from Nasir Bagh, Lahore. The samples were observed under the microscope and were taxonomically identified. Three microalgal species were selected for monoculture. The species were Scenedesmus obliquus, Chlorella vulgaris and Nitzschia scalpelliformis. The microalgae were separated from other microalgae with the help of Pasteur pipette. For monoculturing CHU # 10 medium was selected and growth was checked. Results unveiled that Scenedesmus obliquus showed higher biomass weight on 9th day of inoculation that is 1.00g. it was decreased from 12th to 21st day (0.90 to 0.06). Growth rate was also decreased from -0.006 to -0.027. The net weight gain of algal biomass of Scanadesmus obliques is 0.50g and total growth g/day of it is 0.002. In case of Chlorella vulgaris. Results showed highest biomass weight on 12th day of inoculation that is 1.13 g. It was decreased from 15th to 21st day (1.00 g to 0.48 g).  The growth rate was decreased from -0.0001 to -0.009 (15th to 21st day). The net weight gain is 0.6 g and total growth rate g/day is 0.006. In case of Nitzschia scalpelliformis results showed higher biomass weight (1.48 g) on 15th day. It was decreased (1.07 to 0.50) from 18th to 21st day. The growth rate decreased from 15th to 21st day (0.0005 to -0.001). Net weight gain of algal biomass is 1.105g and total growth rate (g/day) is 0.0006.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44080718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SUPERIORITY OF NANO-SILVER NITRATE AND NANO-CHITOSAN IN CONTROLLING BACTERIAL CONTAMINATION AND PROMOTING GROWTH OF In vitro DATE PALM CULTURES","authors":"Lamiaa Abogarra, N. Eisa, G. El-Habbaa, R. Darwesh, M. El-habbak","doi":"10.56557/pcbmb/2022/v23i33-347845","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i33-347845","url":null,"abstract":"Date palm (Phoenix dactylifera L.) a perennial monocotyledonous tree it’s have an ecological importance and economical role in several countries of Middle East and North Africa, date palm can’t be propagate by seeds, meanwhile the offshoots produced low numbers of offshoots for the given period, so tissue culture technique become a great important for produced the huge demand plantlets, the in vitro technique facing a problem as the contamination by bacterial and fungus that lead to loss the cultures, for eliminate this problem many treatments were used as chitosan 5 and 15 ml/l, AgNO3 4.0 and 7.0 ml/l, Zn 4.0 and 7.0 mg/l and cu 0.5 and 1.0 mg/l. as the main forms or as a nanoparticles NPs in addition to streptomycin 40 and 50 mg/l and AgNO3 + Sodium thiosulfate 0.5 and 1.0 ml/l were added to rooting stage medium for 3 subcultures for each, then these plantlets were acclimatized under greenhouse condition. Collected data were subjected to analysis of variance (ANOVA) and the means were compared following t-test using LSD values at 5%. The percentage of cultures contamination was to be found zero % with the treatments chitosan, silver nitrate AgNO3, in addition to the combination of Chitosan + AgNO3, chitosan + Zn and finally chitosan + Cu at all tested levels produced, while the treatments Zn and Cu at two tested levels produced 11% contamination, the observation of the vegetative growth and leaves contents of indoles and amino acids mg/g f.w. were found highest values under chitosan and AgNO3 and the combination in between as the main forms or as NPs treatments, as well as, the acclimatization stage presented the great survival percentage % under Chitosan and AgNO3 and combination in between as the main form or as NPs. The present study have been suggest that NPs chitosan, AgNO3 and the combination in between might be a promising method for reduced or eliminate the in vitro microbes,  reduced the harmful effects on the valuable date palm plantlets and increasing growth parameters.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47601666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}