Plant Cell Biotechnology and Molecular Biology最新文献_第7页

Induction of Systemic Resistance (ISR) in Ground Nut Against Collar Rot (Aspergillus niger) by Using Pyraclostrobin 20% Water Dispersible Grangules 20%吡唑菌酯水分散颗粒诱导花生对黑曲霉的全身抗性

Plant Cell Biotechnology and Molecular Biology Pub Date : 2023-04-18 DOI: 10.56557/pcbmb/2023/v24i1-28207

S. Sundaramoorthy, J. J. Subakar Ivin

{"title":"Induction of Systemic Resistance (ISR) in Ground Nut Against Collar Rot (Aspergillus niger) by Using Pyraclostrobin 20% Water Dispersible Grangules","authors":"S. Sundaramoorthy, J. J. Subakar Ivin","doi":"10.56557/pcbmb/2023/v24i1-28207","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i1-28207","url":null,"abstract":"An investigation was performed In vitro condition to check induction of systemic resistance in ground nut with Pyraclostrobin 20% WDG (at different concentrations), Vitavax (Carboxin+Thiram 75%WS), Mancozeb 3 g/kg (75% WP), Carbendazim 50% WP, Trichoderma viride, Pseudomonas fluorescens, and Untreated Control treatments. Groundnut seeds sown in pot soil mixed with the inoculum of A.niger at 5% level alone served as control. The treatments were given as per the schedule and foliar spray was given at 20% DAS. The experiment was conducted with three replications in a completely randomized design. All observations viz., the collar rot disease incidence (PDI- at 30 DAS), plant height (cm), plant biomass and pod yield was assessed and recorded at harvest following standard procedures. The results showed that all the treatments significantly reduced the collar rot disease incidence compared to the control. Among the treatments, seed treatment plus foliar spray of Pyraclostrobin 20% WDG at 0.15 % (T3) recorded significantly the least collar rot incidence of 14.12 per cent, maximum germination per cent (93.28%) and maximum yield (132.23 g/pot) than other treatments. The dosage level with Pyraclostrobin 20% WDG as seed treatment @ 1.0 g/kg of seed plus foliar spray at the conc. of 0.1% recorded statistically on par results with T3 the best treatment","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41972161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative Potentials of Methanol Bark Extract of Detarium senegalense (Tallow Tree) in Streptozotocin Induced Diabetic Male Wistar Rats 塞内加尔Detarium senegalense（Tallow Tree）甲醇树皮提取物对链脲佐菌素诱导的糖尿病雄性Wistar大鼠的改善作用

Plant Cell Biotechnology and Molecular Biology Pub Date : 2023-04-10 DOI: 10.56557/pcbmb/2023/v24i1-28197

Odeghe Hope Enameguonor, O. A. Emeka, Ossai Nduka Richard, E. Mamerhi

{"title":"Ameliorative Potentials of Methanol Bark Extract of Detarium senegalense (Tallow Tree) in Streptozotocin Induced Diabetic Male Wistar Rats","authors":"Odeghe Hope Enameguonor, O. A. Emeka, Ossai Nduka Richard, E. Mamerhi","doi":"10.56557/pcbmb/2023/v24i1-28197","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i1-28197","url":null,"abstract":"Diabetes is a global metabolic disease that affects millions of individuals worldwide and raises the global death rate, posing a public health concern. Because antidiabetic traditional drugs are expensive, have side effects, and are ineffective, researchers are looking into novel medicinal plants to find a long-term cure that is inexpensive, has few or no side effects, and is highly effective in treating diabetes and its consequences. Detarium senegalense is a versatile plant used to cure a variety of ailments, including diabetes. This research aimed to investigate the ameliorative potentials of methanol bark extract of Detarium senegalense in streptozotocin-Induced Diabetic male Wistar Rats. Thirty (30) adult male Wistar rats weighing 120-180gm were chosen at random and put into six (6) groups of five rats each (n=5) for this investigation. A single intraperitoneal injection of streptozotocin (150mg/kg) produced diabetes in rats. Normal and diabetic control groups are represented by groups 1 and 2, respectively. Group 3, a diabetic group, received metformin (50mg/kg). Groups 4, 5, and 6 received methanol bark extract of Deterium senegalenses at 250, 500, and 1000 mg/kg/day, respectively. The animals were euthanized at the end of the 21-day treatment period, and organs were excised for biochemical examination. The generated data was analyzed with the SPSS software, and the results were expressed as mean SEM. Result confirms Detarium senegalense methanol bark extract as a hypoglycemic agent on the diabetic rats, by decreasing blood glucose level and the activity of metabolic enzymes (α-amylase and α-glucosidase), increase hexokinase, Superoxide dismutase, Catalase, Glut 1 and Glut 2 activities while decreasing the activities of Malondialdehyde. Conclusively, the bark of Detarium senegalense has been shown to have anti-diabetic and anti-oxidative.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44431177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Characterization of a Bipartite Begomovirus and an Alphasatellite Associated with Leaf Curl and Mosaic Disease of Bitter Gourd in Punjab 旁遮普邦一株与苦瓜叶片卷曲和花叶病相关的双裂秋海棠病毒及其α卫星的分子特征

Plant Cell Biotechnology and Molecular Biology Pub Date : 2023-04-06 DOI: 10.56557/pcbmb/2023/v24i1-28188

P. Lager, J. Sharma, Yogesh Kumar

{"title":"Molecular Characterization of a Bipartite Begomovirus and an Alphasatellite Associated with Leaf Curl and Mosaic Disease of Bitter Gourd in Punjab","authors":"P. Lager, J. Sharma, Yogesh Kumar","doi":"10.56557/pcbmb/2023/v24i1-28188","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i1-28188","url":null,"abstract":"Bitter gourd (Momordica charantia) is an economically important vegetable and medicinal plant that gets infected by a number of viruses. A survey was conducted in and around Jalandhar region of Punjab, India to study viral symptoms in the bitter gourd fields. The plants showed reduced fruit size, upward leaf curling and mosaic symptoms, indicating begomovirus infection. DNA was isolated from the symptomatic leaves and tested for begomovirus infection using group-specific PCR primers. Amplified products were cloned into pMD20-T vector and sequenced. Based on the obtained sequence, new sets of primers were designed and used for amplification of complete DNA-A and DNA-B. Complete DNA-A and DNA-B were cloned in pMD20-T and sequenced. Sequence analysis of the present DNA-A and DNA-B showed their highest identity with Tomato leaf curl New Delhi virus (ToLCNDV). The associated Tomato leaf curl New Delhi alphasatellite was amplified using universal primers, cloned and sequenced. In phylogenetic analysis, the present DNA-A and DNA-B grouped with ToLCNDV isolates and other begomoviruses reported from bitter gourd. Phylogenetic analysis of the present alphasatellite showed its closet identity with Tomato leaf curl New Delhi alphasatellite. Recombination analysis of the DNA-A sequence showed that the present bitter gourd isolate might be a recombinant of Squash leaf curl China virus (SLCChV) as a major parent and Chilli leaf curl virus as a minor parent. Analysis of the DNA-B suggested it to be a recombinant of SLCChV as a major parent and Chyaote enation yellow mosaic virus as a minor parent. The present alphasatellite might be a recombinant of Tomato yellow spot alphasatellite as a major parent and Croton yellow mosaic alphasatellite as a minor parent. The percent identities of the nucleotide and amino acid sequences validate and support the results of the phylogenetic and recombination analyses.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41410966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zinc Effects on Antioxidative Enzymes and Nitrate Reductase Activity in Phaseolus vulgaris (Common Bean) Seedling 锌对菜豆幼苗抗氧化酶和硝酸还原酶活性的影响

Plant Cell Biotechnology and Molecular Biology Pub Date : 2023-04-05 DOI: 10.56557/pcbmb/2023/v24i1-28185

Shaishavee Saini, R. Gadre

{"title":"Zinc Effects on Antioxidative Enzymes and Nitrate Reductase Activity in Phaseolus vulgaris (Common Bean) Seedling","authors":"Shaishavee Saini, R. Gadre","doi":"10.56557/pcbmb/2023/v24i1-28185","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i1-28185","url":null,"abstract":"The present study analyses antioxidative enzymes and in vitro NR activity in common bean seedlings in response to ZnCl2 supply over the range of 0 – 1 mM concentrations. Bean seedlings were grown in plastic pots containing acid washed sand for 10 days and watered daily with ½ Strength Hoagland’s Solution containing 5 mM NH4NO3 as nitrogen source and desired concentration of ZnCl2. Zn supply increased seedling weight and shoot length at 0.001 mM whereas root length decreased at all concentrations. \u0000Amongst the antioxidative enzymes, super oxide dismutase (SOD) activity increased up to 0.1 mM significantly and then decreased at 1 mM in leaf tissue whereas in root tissue it decreased at 0.001 mM and then increased significantly. The catalase (CAT) activity increased at all the concentration being more prominent at 0.1 mM in leaf whereas in roots it increased substantially at 0.1 and 1 mM Zn. Ascorbate peroxidase (APX) activity in leaf increased at 0.1 mM but decreased at 0.001 and 1 mM, whereas in roots it decreased at all the concentration. Various in vitro activities of NR were analysed in response to Zn supply. The effect of Zn on NADH-NR is more substantial than MVH-NR at all the concentrations. Also NRmax and NRact increased at all the concentration of Zn being most prominent at 0.1 mM in leaf and at 1 mM in root tissue.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42489556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antihyperglycemic and Anticholesterolemic Effects of Fig Leaf (Ficus glomerata Linn.) Extract in Alloxan Induced Diabetes in Wistar Albino Rats 无花果叶(Ficus glomerata Linn.)的降血糖和降胆固醇作用四氧嘧啶诱导Wistar白化大鼠糖尿病的提取物

Plant Cell Biotechnology and Molecular Biology Pub Date : 2023-03-22 DOI: 10.56557/pcbmb/2023/v24i1-28169

Sanjeev Kumar, N. K. Prasad, A. Kumari, K. Shachi, N. Dubey

{"title":"Antihyperglycemic and Anticholesterolemic Effects of Fig Leaf (Ficus glomerata Linn.) Extract in Alloxan Induced Diabetes in Wistar Albino Rats","authors":"Sanjeev Kumar, N. K. Prasad, A. Kumari, K. Shachi, N. Dubey","doi":"10.56557/pcbmb/2023/v24i1-28169","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i1-28169","url":null,"abstract":"In this present scenario,medicinal plants are becoming popular for the treatment of various diseases all over the world. Some of them are being traditionally used in diabetes for human welfare. Diabetes is a common endocrine disorder that impairs glucose metabolism resulting in severe diabetic complications including retinopathy, angiopathy, nephropathy, neuropathy and causing neurological disorder due to perturbation in utilization of glucose. In the present study, an effort has been made to assess the antidiabetic properties of Ficus glomerata leaf extract treatment in alloxan monohydrate induced diabetic albino rats. Present study refers to the medicinal plant Ficus glomerata leaf metabolites compared to insulin treatment animal model. Diabetes was induced in albino rats with the help of alloxan monohydrate (150 mg/kg) intraperitoneal saline injection. Present study was carried out to evaluate the anti-diabetic activity of Ficus glomerata aqueous leaf extract in diabetic albino rats for 4 week treatment. The treatment with aqueous leaf extract at high dose (1g/kg) significantly decreased circulating blood glucose concentration in comparison to mild dose (500mg/kg) and low dose (100mg/kg) respectively in diabetic rats. The aqueous leaf extract also improved the hematological picture, weight, biochemical profile and glucose intolerance lowering effects in diabetic animal models. In conclusion, Ficus glomerata leaf metabolites have tremendous antidiabetic potential in alloxan-induced experimental models. The results suggest the possibility that Ficus glomerata leaves metabolites to cure diabetes in herbal medicine.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47551653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Variability in Phaeoisariopsis personata Causing Late Tikka Leaf Spot of Ground Nut in Cuddalore District of Tamil Nadu 泰米尔纳德邦Cuddalore地区导致花生晚Tikka叶斑病的人褐藻分子变异

Plant Cell Biotechnology and Molecular Biology Pub Date : 2023-01-21 DOI: 10.56557/pcbmb/2023/v24i1-28147

S. Sundaramoorthy, J. S. Ivin

引用次数: 0

Medicinal Properties in Some Components of Cannabis Species 大麻某些成分的药用特性

Plant Cell Biotechnology and Molecular Biology Pub Date : 2023-01-10 DOI: 10.56557/pcbmb/2023/v24i1-28094

.. Sonam, M. D. Joshi

引用次数: 0

Selection Procedures for Improving Some Economic Characters and Correlated Responses in Cotton (Gossypium barbadense L.) Cross 棉花(Gossypium barbadense L.)某些经济性状改良及相关响应的选择方法交叉

Plant Cell Biotechnology and Molecular Biology Pub Date : 2023-01-05 DOI: 10.56557/pcbmb/2023/v24i1-28091

H. Hamed, Soad H. Hafez, Ashraf Darwesh, M. Abdel-Moneam

{"title":"Selection Procedures for Improving Some Economic Characters and Correlated Responses in Cotton (Gossypium barbadense L.) Cross","authors":"H. Hamed, Soad H. Hafez, Ashraf Darwesh, M. Abdel-Moneam","doi":"10.56557/pcbmb/2023/v24i1-28091","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i1-28091","url":null,"abstract":"A study was done at Sakha Agricultural Research Station Farm, Kafr El-Sheikh, Egypt during 2019-2021 growing seasons. The study aimed at assessing the efficacy and usability of various selection procedures, determining the effectiveness of selection for superior families and estimating the response to selection. The materials utilized for selection of promising families in early segregating generations included F2, F3 and F4 generations of Giza 94 × S106 cotton cross.. Findings revealed better F4 means than the F3 and F2 means for all studied characters due to the selection procedures used. High heritability values were recorded for most studied characters. Significant desirable correlations were noticed between seed cotton yield with lint yield, bolls/plant, lint/seed, seeds/boll and boll weight. Canonical discriminant analysis among F3 families showed that the first five canonical functions accounted for 100% of total variances. While first canonical discriminant function represented 46% of the total variance among genotypes with the greatest Eigen value and prevailed by great loading from most yield characters and micronaire reading. Ten of the selection indices surpassed direct selection in improving lint yield. The highest actual advance in lint yield for F3 obtained from selection index comprising lint yield/plant in addition to bolls/plant, seeds per boll and lint per seed. In F4 generation, the maximum actual gains were obtained for most yield traits with indices involved lint yield/plant and seeds/boll with lint/seed. Most of the selected families in F4 scored high values for almost all the studied characters and surpassed the corresponding means of selected families in F3 and F2 generations; these selected families might be used to improve cotton yield and fiber quality in breeding programs.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48291617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FIRST REPORT AND GENOME CHARACTERIZATION OF Pepper leaf curl Bangladesh virus INFECTING CHILLI IN INDIA 孟加拉辣椒卷叶病毒在印度感染儿童的首次报告及基因组特征

Plant Cell Biotechnology and Molecular Biology Pub Date : 2022-12-31 DOI: 10.56557/pcbmb/2022/v23i39-408075

J. Sharma, P. Lager, Rahul Kumar, Yogesh Kumar

{"title":"FIRST REPORT AND GENOME CHARACTERIZATION OF Pepper leaf curl Bangladesh virus INFECTING CHILLI IN INDIA","authors":"J. Sharma, P. Lager, Rahul Kumar, Yogesh Kumar","doi":"10.56557/pcbmb/2022/v23i39-408075","DOIUrl":"https://doi.org/10.56557/pcbmb/2022/v23i39-408075","url":null,"abstract":"Chilli (Capsicum annuum L.) leaves showing curling symptoms were collected from Jalandhar region of Punjab, India, and a monopartite begomovirus Pepper leaf curl Bangladesh virus (PepLCBV) associated with Tomato leaf curl Bangladesh betasatellite (ToLCBB) was found to infect the crop. The viral DNA was amplified, cloned and sequenced. Sequencing results revealed that the virus genome and betasatellite consists of 2755 and 1361 nucleotides respectively. Infectious clones of the virus were constructed in binary vector pCAMBIA-1300 and Koch’s postulates were fulfilled. PepLCBV infectious clone caused minor curling in chilli plants when inoculated alone, but it produced typical leaf curl and stunting symptoms in combination with the ToLCBB infectious clone. Phylogenetic analysis with other closely related begomoviruses showed that the present isolate shares high identity with Pepper leaf curl Bangladesh virus. The present isolate grouped with other chilli isolates of begomoviruses reported from India (Pepper leaf curl Lahore virus, Chilli leaf curl Nagpur virus and Tomato leaf curl Liwa virus). Phylogenetic analysis of betasatellite showed its closest identity with Tomato leaf curl Bangladesh betasatellite. It grouped with other betasatellites reported from India (Tomato leaf curl betasatellite, Fenugreek leaf curl betasatellite and Chilli leaf curl betasatellite). Recombination analysis of the virus and betasatellite sequences suggested that PepLCBV might be a recombinant of Chilli leaf curl virus as a major parent and Chilli leaf curl Multan virus as a minor parent, whereas ToLCBB seemed to be a recombinant of Tomato leaf curl betasatellite as a major parent and Tomato leaf curl Ranchi betasatellite as a minor parent. To the best of our knowledge this is the first report of PepLCBV infecting chilli in India.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47851999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SURVEY AND ESTIMATION OF YIELD LOSS DUE TO STEM NECROSIS VIRUS OF POTATO GROWING AREAS OF SOUTH-EASTERN RAJASTHAN 拉贾斯坦邦东南部马铃薯种植区茎坏死病毒产量损失的调查与估算

Plant Cell Biotechnology and Molecular Biology Pub Date : 2022-12-31 DOI: 10.56557/pcbmb/2022/v23i39-408069

M. Meena, D. L. Yadav, H. Meena, C. Meena, R. Yadav

引用次数: 0