A. Kandil, M. Abdel-Moneam, Hashad Soad, S. G. Hasnaa
{"title":"面包小麦基因作用测定、遗传相关性及产量及产量组成因素预测的三重试验交叉分析","authors":"A. Kandil, M. Abdel-Moneam, Hashad Soad, S. G. Hasnaa","doi":"10.56557/pcbmb/2022/v23i39-408013","DOIUrl":null,"url":null,"abstract":"In two crosses of bread wheat (Gemmeiza11 x Misr1 and Gemmeiza12 x Misr2) to investigate gene action, genetic correlations and predicating new recombinant lines for days to heading, plant height, flag leaf area, number of spikes/plant, number of grains/spike, 1000-grain weight, spike grain weight and grain yield /plant, triple test cross was used. The starting materials for triple test cross was the F1 and their grand parents (P1 and P2) for the two studied wheat crosses. The first cross Gemmeiza 11 x Misr1 and the second cross was Gemmeiza 12x Misr2. The F1’s were selfed to produce F2’s grains. In order to create F1 and F2 plants, the obtained materials (F1 and F2 grain genotypes) and the parental genotypes were seeded together. Thirty individual F2 plants were randomly labeled from each cross and crossed back to their grand parents (P1 and P2) and F1 between then to produce three types of families L1 (F2i x P1i), L2 (F2i x P2i) and L3 (F2i x F1i) in each cross. On November 10, 2018, during the winter growing season, the triple test cross families (L1, L2 and L3), along with the parents, F1 and F2 in each cross, were seeded. Results showed that in the genetic system for the investigated features for two crosses, epistatic gene effects were significant. The types of epistasis (additive x additive, additive x dominance and dominance x dominance) were significant for all studied characters. For the majority of traits in both crossings, both additive and dominant genetic components were significant and involved in the genetic system. The average level of dominance for spikes/plant in cross 1 and spike grain weight in cross -2 was overdominance, while for the remaining characters, additive gene effects were more pronounced. The F value was positive and significant for 1000-grain weight in two crosses, suggesting that dominant genes controlling these characters were unidirectional. The highest proportion of inbreds excepted to outperform parental rang in cross 1 for days to heading and flag leaf area. In both crosses, grain yield per plant was positively and significantly correlated with number of spikes per plant, number of grains per spike, and 1000-grain weight, according to epistasis, additive, and dominance genetic correlations.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"TRIPLE TEST CROSS ANALYSIS FOR DETERMINING GENE ACTION, GENETIC CORRELATIONS AND PREDICTION FOR YIELD AND YIELD COMPONENTS IN BREAD WHEAT\",\"authors\":\"A. Kandil, M. Abdel-Moneam, Hashad Soad, S. G. Hasnaa\",\"doi\":\"10.56557/pcbmb/2022/v23i39-408013\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In two crosses of bread wheat (Gemmeiza11 x Misr1 and Gemmeiza12 x Misr2) to investigate gene action, genetic correlations and predicating new recombinant lines for days to heading, plant height, flag leaf area, number of spikes/plant, number of grains/spike, 1000-grain weight, spike grain weight and grain yield /plant, triple test cross was used. The starting materials for triple test cross was the F1 and their grand parents (P1 and P2) for the two studied wheat crosses. The first cross Gemmeiza 11 x Misr1 and the second cross was Gemmeiza 12x Misr2. The F1’s were selfed to produce F2’s grains. In order to create F1 and F2 plants, the obtained materials (F1 and F2 grain genotypes) and the parental genotypes were seeded together. Thirty individual F2 plants were randomly labeled from each cross and crossed back to their grand parents (P1 and P2) and F1 between then to produce three types of families L1 (F2i x P1i), L2 (F2i x P2i) and L3 (F2i x F1i) in each cross. On November 10, 2018, during the winter growing season, the triple test cross families (L1, L2 and L3), along with the parents, F1 and F2 in each cross, were seeded. Results showed that in the genetic system for the investigated features for two crosses, epistatic gene effects were significant. The types of epistasis (additive x additive, additive x dominance and dominance x dominance) were significant for all studied characters. For the majority of traits in both crossings, both additive and dominant genetic components were significant and involved in the genetic system. The average level of dominance for spikes/plant in cross 1 and spike grain weight in cross -2 was overdominance, while for the remaining characters, additive gene effects were more pronounced. The F value was positive and significant for 1000-grain weight in two crosses, suggesting that dominant genes controlling these characters were unidirectional. The highest proportion of inbreds excepted to outperform parental rang in cross 1 for days to heading and flag leaf area. 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引用次数: 0
摘要
以两个面包小麦杂交组合(Gemmeiza11 × Misr1和Gemmeiza12 × Misr2)为材料,采用三联试交,研究基因作用、遗传相关性和预测重组品系对抽穗、株高、面叶面积、穗数、穗数、千粒重、穗粒重和单株产量的影响。所研究的两个小麦杂交组合均以F1及其祖代P1和P2为起始材料。第一个杂交品种是gemmeiiz11 × Misr1,第二个杂交品种是gemmeiiz12 × Misr2。F1被自交以产生F2的籽粒。将获得的材料(F1和F2粒型)与亲本基因型一起播种,以培育F1和F2植株。从每个杂交中随机标记30个F2植株,并将其交回其祖父母(P1和P2)和他们之间的F1,在每个杂交中产生L1 (F2i x P1i), L2 (F2i x P2i)和L3 (F2i x F1i)三种类型的家族。2018年11月10日,在冬季生育季节,将三个试验杂交家族(L1、L2和L3)与每个杂交的亲本F1和F2一起播种。结果表明,在两个杂交组合所研究性状的遗传系统中,上位基因效应显著。所有性状的上位性类型(加性x加性、加性x显性和显性x显性)均显著。在两个杂交组合的大多数性状中,加性遗传成分和显性遗传成分均显著参与遗传系统。杂交1穗/株的平均显性水平和杂交2穗粒重的平均显性水平均为显性,其余性状的加性基因效应更为显著。两个杂交的千粒重F值均为正且显著,说明控制这些性状的显性基因是单向的。自交系除在抽穗和旗叶面积上表现优于亲本外,在交代1中占比最高。在两个杂交组合中,单株籽粒产量与单株穗数、穗粒数和千粒重在上位性、加性和显性遗传相关方面均呈显著正相关。
TRIPLE TEST CROSS ANALYSIS FOR DETERMINING GENE ACTION, GENETIC CORRELATIONS AND PREDICTION FOR YIELD AND YIELD COMPONENTS IN BREAD WHEAT
In two crosses of bread wheat (Gemmeiza11 x Misr1 and Gemmeiza12 x Misr2) to investigate gene action, genetic correlations and predicating new recombinant lines for days to heading, plant height, flag leaf area, number of spikes/plant, number of grains/spike, 1000-grain weight, spike grain weight and grain yield /plant, triple test cross was used. The starting materials for triple test cross was the F1 and their grand parents (P1 and P2) for the two studied wheat crosses. The first cross Gemmeiza 11 x Misr1 and the second cross was Gemmeiza 12x Misr2. The F1’s were selfed to produce F2’s grains. In order to create F1 and F2 plants, the obtained materials (F1 and F2 grain genotypes) and the parental genotypes were seeded together. Thirty individual F2 plants were randomly labeled from each cross and crossed back to their grand parents (P1 and P2) and F1 between then to produce three types of families L1 (F2i x P1i), L2 (F2i x P2i) and L3 (F2i x F1i) in each cross. On November 10, 2018, during the winter growing season, the triple test cross families (L1, L2 and L3), along with the parents, F1 and F2 in each cross, were seeded. Results showed that in the genetic system for the investigated features for two crosses, epistatic gene effects were significant. The types of epistasis (additive x additive, additive x dominance and dominance x dominance) were significant for all studied characters. For the majority of traits in both crossings, both additive and dominant genetic components were significant and involved in the genetic system. The average level of dominance for spikes/plant in cross 1 and spike grain weight in cross -2 was overdominance, while for the remaining characters, additive gene effects were more pronounced. The F value was positive and significant for 1000-grain weight in two crosses, suggesting that dominant genes controlling these characters were unidirectional. The highest proportion of inbreds excepted to outperform parental rang in cross 1 for days to heading and flag leaf area. In both crosses, grain yield per plant was positively and significantly correlated with number of spikes per plant, number of grains per spike, and 1000-grain weight, according to epistasis, additive, and dominance genetic correlations.