Plant Cell Biotechnology and Molecular Biology最新文献

{"title":"Effect of Pomegranate (Punica granatum) Peel Powder Supplementation on Hemato-Biochemical Profile of Lactating Mehsana Goats","authors":"C. P. Modi, S. S. Patil, M. M. Pawar, S. K. Mohapatra, H. H. Panchasara, Mayank Patel, J. R. Patel, B. K. Ashwar","doi":"10.56557/pcbmb/2023/v24i5-68404","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i5-68404","url":null,"abstract":"This study was conducted to evaluate the effect of supplementation of pomegranate peel powder on hemato-biochemical profile of lactating Mehsana goats. Twenty-four lactating Mehsana goats (7 days post- partum) were assigned randomly into four groups (6 animals in each) and stratified on the basis of their live weight and milk production in previous lactation. The experimental animals in T1 group were fed on basal diet while in T2, T3 and T4 groups were supplemented with pomegranate peel powder at the rate of 10, 20 and 40 g per day per head, respectively. The duration of this experiment was 120 days. The blood samples were collected at the end (120th day) of experimental feeding. The mean hemoglobin and packed cell values were non-significant among the experimental groups (the T1, T2, T3 and T4 groups). Likewise, there was no effect on TEC and TLC caused by the dietary addition of pomegranate peel powder in lactating Mehsana goats. The difference in serum biochemical parameters viz. glucose, total proteins, albumin, triglycerides, cholesterol, ALT, AST and urea were non-significant among T1, T2, T3 and T4 groups. The serum creatinine concentration was significantly (P<0.05) higher in groups T2 and T3 than the T1 and T4 groups. Based on the results, it may be concluded that pomegranate (Punica granatum) peel powder can be supplemented in the diet of lactating Mehsana goats up to the rate of 40 g/day/animal during the period of 120 days post-partum without any adverse effects on hemato-biochemical parameters.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135483749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial, Antimitotic and Anti-Inflammatory Efficacy of Delonix regia Flower Extracts","authors":"None Hariharan P., Aditi A. Iyengar, Shriya Anand Bhambore, None Arul Selvan R., None Nagendra H. G.","doi":"10.56557/pcbmb/2023/v24i5-68384","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i5-68384","url":null,"abstract":"Delonix regia is a multipurpose tree, it is harvested from the wild for a wide range of local uses, including for medicines, food, timber, fuel and beads. It is cultivated as a shade tree in plantations and is used to stabilize and enrich the soil. This species is one of the most widely cultivated ornamental plants in the world, being grown as an ornamental plant in gardens and by the sides of roads throughout the tropics. The main objective of the study was to understand the effect of the Delonix regia flower extract for its antibacterial, anti-inflammatory and antimitotic study. For the analysis of the antibacterial study, the ethanolic extraction method was used and the extract was dissolved in DMSO and distilled water which was further tested on the clinical strains of Staphylococcus and Enterococcus faecalis, where zone of inhibition was observed for both the strains. For the analysis of the anti-inflammatory study human blood was used, the inflammatory test was carried by the Hemolysis Inhibition Assay, post the analysis the extract showed potential to inhibit the breakdown of red blood cells. For the analysis of antimitotic activity Macrotyloma uniflorum (horse gram) was suspended in the chemotherapy drug- Vinblastin along with control and dilutions of the flower extract, which was quantitatively assessed. The results implied that the flower extract has properties that can inhibit the growth of cells/gram.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135784928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct and Indirect Organogenesis in Olive (Olea europaea L.)","authors":"Firoozeh Chamandoosti","doi":"10.56557/pcbmb/2023/v24i5-68383","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i5-68383","url":null,"abstract":"Olive explants included of pieces of young stems which were divided into nodal explants cultured on MS media supplemented with cytokinins and auxin. Callus induction and different forms of shooting and adventitious lateral buds regeneration were dominant results. Level of auxin IBA in all treatments was used at 0.1 mg/l. But cytokinins combination were used at different combinations. Combination of BA and ZEA led to more organogenesis and combination of BA and 2ip led to callus induction. Organogenesis was occurred with three different forms including indirect with passing callus phase, direct with growth of lateral buds and elimination of dominance apical and direct with regeneration of new lateral buds on explants.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135783795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Advanced Lines of Aus Rice (Oryza sativa) Based on Qualitative Traits","authors":"Aliya Adiba Khanam, Md. Shahidur Rashid Bhuiyan, Md. Sarowar Hossain, Ahmed Shahriar Anik, Mst. Sumayea Khan, Kamrun Nahar Sheuly, Md. Imdadul Haque, Khalid Syfullah","doi":"10.56557/pcbmb/2023/v24i5-68375","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i5-68375","url":null,"abstract":"The present study was conducted at the experimental farm, Department of Genetics and Plant Breeding, Sher-e-Bangla Agricultural University, Dhaka during Aus season, 2017. The experiment was carried out to characterize ten advanced Aus lines (F8) based on qualitative traits. The lines were evaluated in RCBD with three replications. Thirty one qualitative traits were used to characterize the advanced lines. Variation was observed in the traits like leaf pubescence, shape of the ligule, attitude of the blade of flag leaf, days to heading, days to maturity, 1000-grain weight, grain length (without dehulling), sterile lemma length, decorticated grain length (after dehulling), decorticated grain shape, culm length, panicle length. Minimum days to heading was found in L1, L2, L3, L4, L5, and L10. Early maturity was found in L1, L2, L3, L4, L5, and L10. 1000 grain wt. was highest in L2, L4, L6, L7, and L8. Six lines (L1, L2, L3, L4, L5, and L10) showed early time of heading, early maturing and medium type of culm length. Two lines (L7, L9) were observed having long panicle (26-30 cm). It was concluded that the lines L10, L5, L3 and L4 can be selected for further study.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135885440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Antimicrobial Properties of Curcuma longa L. (Turmeric) Leaf Extract and Its Potential Application in Consumer Goods","authors":"None Hariharan P., None Prashant S. H., None Chaturthy, None Trisha C., None Priyanka R., None Nagendra H. G.","doi":"10.56557/pcbmb/2023/v24i5-68374","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i5-68374","url":null,"abstract":"Turmeric has been used as a functional food material but, other parts of the plant such as leaves are wasted as byproducts. This project aims at utilization of turmeric leaves which is generally thrown off or burnt as waste. Disposal of turmeric leaves after harvest of turmeric possesses a huge challenge for the farmers, removal of leaves from the plant comes with an extra cost as the workers need to be paid for removing the leaves, thus to cut the cost farmers burn the leaves. India dominates the world production scenario contributing 80% of turmeric growth, which is mainly used as spice with other applications being used in preparation of dyes, drugs, cosmetics and for religious ceremonies. Karnataka is the third largest producer of turmeric in India. According to studies, the phytochemical characteristics of the turmeric leaf extract demonstrates functional effects such as cosmeceutical, skin immunity, anti-inflammation, antimicrobial, anti-insecticidal activities and antixidant activities. Turmeric leaves also have large volume and surface area and thus can be used for industrial applications. Turmeric leaf oil is rich in phenolic components. The main constituents of the turmeric leaves are curcumnene, limonene, phellandrene and turmerone. In this regard, this project aims at complete utilization of turmeric leaves and turning the leaves which is generally regarded as waste into value added products. The final products developed from the leaves are Dhoop sticks and Room Freshener. The Dhoop sticks developed are organic and chemical free and has aroma that can be refreshing and relaxing as turmeric leaves have strong aroma. Turmeric leaves are rich in Curcumin which is known to have Anti-insecticidal properties in this regard Dhoop sticks are being developed. Perfumes/Room freshener are developed using turmeric leaves as they have anti-bacterial properties and prevents any type of skin allergy while producing fresh fragrance. The products are developed keeping in the mind the source of extra income they act as for the farmers. When tested for anti-bacterial activity the results gave positive results, which is an indication that turmeric leaves have great applications and needs to be explored.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136023689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation of Combining Ability for Flowering and Yield Attributes in Bhendi (Abelmoschus esculentus (L.) Moench)","authors":"D. Gokilappriya, S. Anuja, R. Kandasamy, T. Sabesan","doi":"10.56557/pcbmb/2023/v24i5-68371","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i5-68371","url":null,"abstract":"The present study on general combining ability effects and specific combining ability effects involves seven parents viz., G1 (Arka Anamika), G2 (Aruna), G3 (Varsha Uphar), G4 (Pusa-5), G5 (MDU-1), G6 (Kashi Kranti), G7 (Ankur-41) with their 21 F1 hybrids through half-diallel mating system. The present investigation was undertaken using Randomized Block Design with three replications. The combining ability effects were estimated for seven characters viz., days to first flowering, days to first fruit harvest, fruit length, fruit girth, fruit weight, number of immature seeds per fruit and fruit yield per plant. The results showed that gca effects of parents and sca effect of hybrids were significantly differed. The SCA variance was higher than the GCA variance that indicates the presence of preponderance of non-additive gene action for all the seven traits i.e., presence of preponderance of non-additive gene action for all characters except number of nodes per plant. Among the parents, G3 was found to be a good general combiner based on the per se performance and gca effects for its flowering and yield traits. Among the 21 F1 hybrids, the crosses G3 x G5 and G4 x G6 were superior on the basis of per se performance and sca effect for flowering and yield attributes. Hence, these hybrids were used for further exploitation of heterosis.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136194002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Bioactive Components and in vitro Cytotoxicity Analysis of Annona squamosa Extract on Human Breast Cancer Cells","authors":"S. Selvakumar, A. Mounisha, J. Joshna Florence","doi":"10.56557/pcbmb/2023/v24i5-68372","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i5-68372","url":null,"abstract":"The present study is to explore to determine the bioactive components and in vitro cytotoxicity of the chloroform extract of Annona squamosa analyzed. Materials and Methods: The aerial parts of chloroform extract of the medicinal plant Annona squamosa were used to regulate the bioactive components and their in vitro analysis of cytotoxicity was ceased by vero and human breast MCF-7 cell lines were analyzed by gas chromatography and mass spectrometry and MTT assay respectively. Results: The present study envisages 12 phytocompounds and their significant cytotoxic role on Vero as well as human breast carcinoma and MCF-7 cell lines. Conclusion: Herbal medicinal plants have been extensively studied for their possible medicinal value as primary source drugs. Obviously, the natural products from the herbal medicinal sources will continue to be extremely important as sources of medicinal agents.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136194008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microsatellite Markers Based Molecular Variability among Locally Collected Cajanus cajan (L.) Millsp. Genotypes","authors":"Niraj Tripathi, Dhaneshwar B. Patil, S. Kakade, Sahab Kumar Patel, Gopi Anjana, Moni Thomas","doi":"10.56557/pcbmb/2023/v24i5-68357","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i5-68357","url":null,"abstract":"Pigeonpea [Cajanus cajan (L.) Millsp.] is an important deep-rooted pulse crop, predominantly cultivated in rainfed areas by small and marginal farmers in India and South Africa. The crop’s productivity is severely constrained due to abiotic and biotic stresses in its agro-ecosystem. Molecular variability assessment in pigeonpea can be the key for successful breeding to mitigate the crop from future biotic and abiotic stresses. Molecular markers based genetic diversity analysis was done to evaluate locally collected 32 genotypes of pigeonpea. Total 20 microsatellite markers were used in the present analysis. Pigeonpea genotypes had higher level of genetic diversity. Microsatellite markers were able to discriminate the studied genotypes into three clusters. Most of the genotypes were grouped together according to their collection sites. The findings of the present study open the window of opportunity,  for future research and exploitation of these locally collected genotypes for pigeonpea crop improvement.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46797239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Iron Nanoparticles Application on Date Palm In-vitro Regeneration Stability","authors":"R. Arafa, SayedA.A. Elsayh, I. S. El-Din, S. El-Habashy, E. Ahmed, E. Afifi, Marwa M. Abdalgaleel, Rabab W. El Aramany, Alaa N. Draz, Mohamed F. Gaber","doi":"10.56557/pcbmb/2023/v24i3-48356","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i3-48356","url":null,"abstract":"The use of nanoparticles is critical in a number of industries, including manufacturing, healthcare, and agriculture. Iron nanoparticles (Fe NPs) are one of the main sources of plant nutrition because they are effective in releasing a variety of pH values. To study the effect of different concentrations of Fe NPs (0, 0.5, 1.0, 2.0, 4.0 ml/l) on the regeneration and rooting of Bartamoda cv. date palm, as well as to ascertain the content of carbohydrates and chlorophyll in shoots, an experiment was conducted at the Central Laboratory for Research and Development of Date Palm, Agricultural Research Center Giza, Egypt. Adding treatments of Fe NPs at 1.0 and 2.0 ml/l to MS medium containing BA at 2.0 mg/l and NAA at 0.5 mg/l significantly enhanced growth (number of shoots/cluster, number of leaves/cluster, and leaf length), according to Results. MS medium supplemented with NAA at 1.0 mg/l and Fe NPs at 1.0 ml/l showed the highest rooting percentage (89.0%). Treating the shoots with varying doses of iron nanoparticles led to a significantly higher iron content compared to the untreated control shoots. The carbohydrate content improved steadily and significantly with increasing Fe NP concentration. Compared with the control group, treatments with Fe NPs at 1.0 and 2.0 ml/l enhanced the levels of chlorophyll a, chlorophyll b, and carotenoids. \u0000Through the use of random amplified polymorphic DNA (RAPD) profiles, the somaclonal variation in shoots that can be caused by Fe NPs during the multiplication stage was examined. To amplify DNA from various shoots, seven random 10-mer primers were applied. The shoots' RAPD patterns matched those of the original plant mother, proving that Fe NPs did not cause somaclonal variation that could be seen using the RAPD method.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47466843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Surface Sterilization for Micropropagation of Gerbera jamesonii: A Study on Explant Survival and Contamination Control","authors":"Shylendra Kumar, S. K. Sehrawat, A. Malik, Shikha Yashveer, Deepak Kumar, Priyesh Kumar","doi":"10.56557/pcbmb/2023/v24i3-48345","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i3-48345","url":null,"abstract":"In the commercial production of gerberas, micropropagation enables the large-scale production of plants in a limited space, as well as the obtaining of disease and pest-free uniform plants. This technique also ensures precision in production schedules and product quality, regarding plant homogeneity and vigour. Nevertheless, the occurrence of contamination in an in vitro culture is frequent, and might result in high damage. Plant materials have different kind of microbes i.e., exogenous and endogenous in nature which is the main reason for contamination in tissue culture. Pre-treatments to explants with fungicides and bactericides can be effectively used to eliminate the surface contamination. Surface sterilizing agents, their levels and duration of exposure are known to impact the in vitro culture establishment. In the present experiment, pre-treatment of explants was carried out with Teepol BleachTM solution (0.1%) and BavistinTM (0.1%) for 15 and 20 minutes respectively for effective control of fungal contaminations in all the cultures. For the control of bacterial contamination antibiotics were added in culture medium. Surface sterilization of explants with 0.1% mercuric chloride (HgCl2) for five minutes gave the maximum survival of the growing cultures.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45743667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}