Plant Cell Biotechnology and Molecular Biology最新文献

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Effect of Different Doses of Nutrients on Vegetative, Reproductive and Biochemical Parameters of Cauliflower (Brassica oleracea L.) cv. F1 Amazing Hybrid 不同剂量的营养元素对花椰菜(Brassica oleracea L.)变种植株、生殖和生化参数的影响F1 神奇杂交种
Plant Cell Biotechnology and Molecular Biology Pub Date : 2024-02-27 DOI: 10.56557/pcbmb/2024/v25i1-28559
Sadam Hussain, Muhammad Irfan Ashraf, Faizan Ahmad Chattha, Rubab Zahra, Muhammad Bilal Khan
{"title":"Effect of Different Doses of Nutrients on Vegetative, Reproductive and Biochemical Parameters of Cauliflower (Brassica oleracea L.) cv. F1 Amazing Hybrid","authors":"Sadam Hussain, Muhammad Irfan Ashraf, Faizan Ahmad Chattha, Rubab Zahra, Muhammad Bilal Khan","doi":"10.56557/pcbmb/2024/v25i1-28559","DOIUrl":"https://doi.org/10.56557/pcbmb/2024/v25i1-28559","url":null,"abstract":"Cauliflower (Brassica oleracea L.) is a well-known winter vegetable crop which belongs to Cruciferae family. Cauliflower needs a large amount of macronutrients as they play role in the development and growth of curd. Therefore, a field experiment was executed to check the different sources of nutrients on vegetative, reproductive and biochemical parameters. Seven different concentrations of macronutrients i.e., Control (no fertilizer), N=150 kg/acre, P=100 kg/acre, K=100 kg/acre, NP=150 + 100 kg/acre, NK=150 + 100 kg/acre and NPK=150 + 100 + 100 kg/acre were applied to cauliflower and experiment was laid out in Randomized Complete Block Design with four replications. Results indicated that maximum values for vegetative and reproductive parameters such as plant height, numbers of leaves per plant, leaf area, foliage fresh weight per plant, foliage dry weight per plant, curd weight, curd diameter, curd yield per hectare and chlorophyll contents (63.2 cm, 21 leaves/plant, 1233.9 cm2, 930 g/plant, 246 g/plant, 695.79 g, 15.26 cm, 25.20 tons/ha and 2.07 CCi respectively) were recorded in NPK treatment (150 + 100 + 100 kg/acre). Whereas, the lowest values were noted in control treatment (no fertilizer). However, total soluble  solids were noted maximum (6.49oBrix and 6.19oBrix) in NK (150+100 kg/acre) and NPK (150 + 100 + 100 kg/acre) treatments and vitamin C was maximum (46.35 mg/100ml and 45.67  mg/100ml) in the treatments NPK (150 + 100 + 100 kg/acre) and NK (150 + 100 kg/acre). From this study, it is concluded that application of NPK (150 + 100 + 100 kg/acre) is the recommended  dose for better growth, curd production and higher quality yield of cauliflower cv. F1 amazing hybrid.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"9 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140426046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Horticultural Innovations Elevating Crop Yields and Agricultural Sustainability for a Flourishing Future 园艺创新提高作物产量和农业可持续性,实现繁荣未来
Plant Cell Biotechnology and Molecular Biology Pub Date : 2024-02-27 DOI: 10.56557/pcbmb/2024/v25i1-28560
Sandeep Indurthi, Ira Sarma, Dokka Vara Vinod
{"title":"Horticultural Innovations Elevating Crop Yields and Agricultural Sustainability for a Flourishing Future","authors":"Sandeep Indurthi, Ira Sarma, Dokka Vara Vinod","doi":"10.56557/pcbmb/2024/v25i1-28560","DOIUrl":"https://doi.org/10.56557/pcbmb/2024/v25i1-28560","url":null,"abstract":"Advancements in horticultural technologies and practices are critical for meeting global food security needs while ensuring environmental sustainability. This paper examines recent innovations that enhance crop yields and resource efficiency in horticulture. Key areas explored include high-tech greenhouse agriculture, precision agriculture techniques, improved irrigation systems, biofertilizers and biopesticides, breeding of resilient cultivars, and vertical farming. Intensive greenhouse production with supplemental lighting, climate control, hydroponics and automation enables year-round vegetable and fruit yields up to 20 times higher than open-field methods. Precision agriculture leverages data analytics, sensors and AI for optimized inputs and cultivation decisions per sub-field zone. Upgraded irrigation systems like drip lines and computerized scheduling curtail water usage. Organic biofertilizers and biopesticides derived from microbes, plants and minerals boost soil health and impede pests while avoiding chemical residues. Newly bred cultivars feature accelerated growth, improved taste and nutrition, and resistance to biotic and abiotic stresses. Meanwhile, vertical farms multilayer indoor cropping to magnify yields in small footprints. Further integration of these and other cutting-edge horticultural technologies can increase global food production without environmental sacrifice. This necessitates greater public and private sector investments paired with institutional support.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"57 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140427285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Baby Corn: A New Challenges, Scope, Present Status and Strategies 婴儿玉米:新挑战、范围、现状和战略
Plant Cell Biotechnology and Molecular Biology Pub Date : 2024-02-13 DOI: 10.56557/pcbmb/2024/v25i1-28542
G. Swapna, G. Jadesha, P. Mahadevu, B. S. Shivakumar, B. T. R. Babu, Mallikarjuna N., Chandrakala Hanagi
{"title":"Baby Corn: A New Challenges, Scope, Present Status and Strategies","authors":"G. Swapna, G. Jadesha, P. Mahadevu, B. S. Shivakumar, B. T. R. Babu, Mallikarjuna N., Chandrakala Hanagi","doi":"10.56557/pcbmb/2024/v25i1-28542","DOIUrl":"https://doi.org/10.56557/pcbmb/2024/v25i1-28542","url":null,"abstract":"Maize is gaining popularity in both food and feed sectors. Every part of corn cob posses’ potential to be used for different purposes, and have economic value. With the changing in living lifestyle, the demand of baby corn is increasing due to its nutritional value, and to be the best alternative income source for farmers. Baby corn is a commercial crop developed in late 70’s, it is used as vegetable crop and it is called as a catch crop, due to its delicious taste and their nutritional health benefits in human beings and its increased demand in market soon it became a cash crops. Being a perishable crop, the shelf life of baby corn is less. It is a good organic food which could be used to prepare wide range of baby corn value added products. The value addition of the baby corn makes its availability in the offseason and also provides employment opportunities to person under poverty line and besides foreign exchange is also possible in both food and feed sectors.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"71 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139841030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Baby Corn: A New Challenges, Scope, Present Status and Strategies 婴儿玉米:新挑战、范围、现状和战略
Plant Cell Biotechnology and Molecular Biology Pub Date : 2024-02-13 DOI: 10.56557/pcbmb/2024/v25i1-28542
G. Swapna, G. Jadesha, P. Mahadevu, B. S. Shivakumar, B. T. R. Babu, Mallikarjuna N., Chandrakala Hanagi
{"title":"Baby Corn: A New Challenges, Scope, Present Status and Strategies","authors":"G. Swapna, G. Jadesha, P. Mahadevu, B. S. Shivakumar, B. T. R. Babu, Mallikarjuna N., Chandrakala Hanagi","doi":"10.56557/pcbmb/2024/v25i1-28542","DOIUrl":"https://doi.org/10.56557/pcbmb/2024/v25i1-28542","url":null,"abstract":"Maize is gaining popularity in both food and feed sectors. Every part of corn cob posses’ potential to be used for different purposes, and have economic value. With the changing in living lifestyle, the demand of baby corn is increasing due to its nutritional value, and to be the best alternative income source for farmers. Baby corn is a commercial crop developed in late 70’s, it is used as vegetable crop and it is called as a catch crop, due to its delicious taste and their nutritional health benefits in human beings and its increased demand in market soon it became a cash crops. Being a perishable crop, the shelf life of baby corn is less. It is a good organic food which could be used to prepare wide range of baby corn value added products. The value addition of the baby corn makes its availability in the offseason and also provides employment opportunities to person under poverty line and besides foreign exchange is also possible in both food and feed sectors.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"120 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139780919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cloning and Characterization of Rep Gene of the Strain of Tomato Leaf Curl New Delhi Virus (G: Begomovirus, F: Geminiviridae) Infecting Ridge Gourd in Karnataka and in silico Analysis of Rep Protein 克隆感染卡纳塔克邦脊瓜的番茄卷叶新德里病毒(G:Begomovirus,F:Geminiviridae)菌株的 Rep 基因并确定其特征,以及 Rep 蛋白质的硅学分析
Plant Cell Biotechnology and Molecular Biology Pub Date : 2023-12-11 DOI: 10.56557/pcbmb/2023/v24i7-88466
Ananya, Nagesha N., Mahesha B.
{"title":"Cloning and Characterization of Rep Gene of the Strain of Tomato Leaf Curl New Delhi Virus (G: Begomovirus, F: Geminiviridae) Infecting Ridge Gourd in Karnataka and in silico Analysis of Rep Protein","authors":"Ananya, Nagesha N., Mahesha B.","doi":"10.56557/pcbmb/2023/v24i7-88466","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i7-88466","url":null,"abstract":"Ridge gourd [Luffa acutangula (Roxb.) L.,] is one of the important vegetable crop affected by various diseases particularly viral disease tomato leaf curl New Delhi virus (ToLCNDV). The ridge gourd plants with leaf curling and yellowing symptoms were collected and used in for the amplification of the of the Rep gene. Cloning, sequencing and bioinformatics analysis of the Rep gene was carried out.  The results revealed the association of this virus isolate with the different host of Cucurbitaceae family. Pairwise identity derived implemented by BLAST identified that the replicator initiator protein gene (Rep) of the bipartite virus showed  maximum 96.90% sequence identity to the corresponding Rep gene of ridge gourd tomato leaf curl New Delhi virus isolate RG3 in ridge gourd - complete genome from Madikari, Karnataka (accession no KT426905). This also showed 97.90% sequence similarities with tomato leaf curl New Delhi virus strain CH7 of Chrysanthemum. Phylogenetic dendrogram of the Rep gene depicted that it grouped with ToLCNDV of different Indian isolates of ridge gourd and with ToLCNDV of potato from Pakistan (LN908936), and with tomato from Bangladesh (KM383738.1) showing 93.12% and 92.94% sequence similarity respectively. This study provides association of the Rep gene infected ridge gourd pants with Cucurbitaceae family crops and other related crops. This will helps in carrying further research in the management of ToLCNDV diseases using different biotechnological applications.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"21 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138980744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protoplasts Isolation from Sardinian Germoplasm of Saffron (Crocus sativus L.) 从撒丁岛藏红花(Crocus sativus L.)种质中分离原生质体
Plant Cell Biotechnology and Molecular Biology Pub Date : 2023-11-29 DOI: 10.56557/pcbmb/2023/v24i7-88450
G. M. Scarpa
{"title":"Protoplasts Isolation from Sardinian Germoplasm of Saffron (Crocus sativus L.)","authors":"G. M. Scarpa","doi":"10.56557/pcbmb/2023/v24i7-88450","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i7-88450","url":null,"abstract":"Genetic studies of saffron indicate low heritability and strong environmental effects for most of the traits examined, suggesting local variability that has been selected over time. In vitro culture can be of support for genetic improvement programs, multiplication, or production of secondary metabolites. This is particularly relevant for saffron due to its triploidy. In the present work, the in vitro culture of Sardinian genotypes of saffron, using protoplasts, is described. Protoplasts were obtained from young leaves using a KAO enzyme mixture and cultured on KM8P substrate. The use of Evans et al. enzymes did not result in the production of protoplasts. The results presented in this work should be considered as the initial phase in the in vitro production of secondary metabolites obtained from local saffron genotypes.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139214560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative Analysis of Pollen Grains from Various Floral Species Using Scanning Electron Microscope 使用扫描电子显微镜比较分析不同花卉品种的花粉粒
Plant Cell Biotechnology and Molecular Biology Pub Date : 2023-11-23 DOI: 10.56557/pcbmb/2023/v24i7-88438
Anukritii Dutta, Vinay Kumar, R. Dutta
{"title":"Comparative Analysis of Pollen Grains from Various Floral Species Using Scanning Electron Microscope","authors":"Anukritii Dutta, Vinay Kumar, R. Dutta","doi":"10.56557/pcbmb/2023/v24i7-88438","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i7-88438","url":null,"abstract":"The morphology of pollen may be used for identification of different species of plants. In this study, pollen morphology and ultrastructure of four cultivars of Moss Rose, Hibiscus rosa sinensis, Petunia and Red Garden Rose were examined using scanning electron microscopy (SEM). The characteristics studied were pollen size and shape (length, width, and length/width ratio), length of the colpus, width of the mesocolpium, and exine characteristics (number of ridges per an area of 100 μm2, width of the ridge, and width of the furrow). All these plant flowers had tricolpate pollen grains and striate exine ornamentation, but significant differences in some traits were found.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"1296 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139243664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Progression of Bacterial Blight of Clusterbean (Cyamopsis tetragonoloba) in Relation to Different Sowing Dates, Varieties and Weather Parameters 不同播种日期、品种和天气参数对四季豆细菌性疫病的影响
Plant Cell Biotechnology and Molecular Biology Pub Date : 2023-11-20 DOI: 10.56557/pcbmb/2023/v24i7-88436
Rahul Raj Bharti, M. Khichar, Mohit Godara, Lalit Mohan, Ankush Kumar, Ajit Singh
{"title":"Progression of Bacterial Blight of Clusterbean (Cyamopsis tetragonoloba) in Relation to Different Sowing Dates, Varieties and Weather Parameters","authors":"Rahul Raj Bharti, M. Khichar, Mohit Godara, Lalit Mohan, Ankush Kumar, Ajit Singh","doi":"10.56557/pcbmb/2023/v24i7-88436","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i7-88436","url":null,"abstract":"At CCS Haryana Agricultural University, Hisar, Haryana, a field study was conducted to investigate the impact of varying planting timings, varieties, and weather parameters on the advancement of bacterial blight in clusterbean. The experiment was set up in three replications using a factorial randomized block design. Three types of cluster beans, namely HG 365, HG 563, and HG 2-20, were sowed at three distinct dates i.e., the 1st, 3rd, and 4th week of July. Clusterbean bacterial blight spread faster in late-planted crops (4th week of July) than in timely-planted (2nd week of July) and early-planted (1st week of July). Maximum temperature (Tmax) and maximum relative humidity (RHm) correlated positively, while minimum temperature (Tmin) and minimum relative humidity (RHe) correlated negatively, and disease development was extremely significant and negatively correlated for HG 365 at all sowing dates. Clusterbean HG 365 was shown to be more resistant to bacterial blight than HG 563 and HG 2-20.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"30 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139256628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization of the Media and Plant Growth Regulators for Clonal Propagation of Adansonia digitata L.: An Endangered Tree 濒危树种adansononia digitata L.无性系繁殖培养基和生长调节剂的优化
Plant Cell Biotechnology and Molecular Biology Pub Date : 2023-11-01 DOI: 10.56557/pcbmb/2023/v24i5-68426
Mujeeb Ur Rehman, Manisha Chaudhary, Sandeep Kumar
{"title":"Optimization of the Media and Plant Growth Regulators for Clonal Propagation of Adansonia digitata L.: An Endangered Tree","authors":"Mujeeb Ur Rehman, Manisha Chaudhary, Sandeep Kumar","doi":"10.56557/pcbmb/2023/v24i5-68426","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i5-68426","url":null,"abstract":"This research carried out to evaluate the influence of different nutrient media, specifically Murashige and Skoog (MS), Anderson medium (AM), and Woody Plant Medium (WPM), in both liquid and semisolid forms, on the in vitro growth of shoots and roots in Adansonia digitata (baobab) using nodal segments as the starting explants. These media were supplemented with varying concentrations of two plant growth regulators, NAA (0.1-1.0 mg/l) and BAP (0.2-2.0 mg/l).The results of the study demonstrated that Adansonia digitata explants exhibited the highest increase in both the number and length of shoots when cultured on MS medium with a combination of NAA (0.3 mg/l) and BAP (0.5 mg/l) in a semisolid state. The average number of shoots reached 3.8±2.33, and their average length was 2.3±2.00 cm. Plantlets grown on MS medium exhibited well-developed leaves and shoots.Further experiments were conducted with in vitro shoots, exploring various concentrations of the auxin NAA and cytokinins BAP, TDZ, and KN on MS medium. The findings indicated that BAP and NAA were more effective in promoting shoot proliferation compared to the other tested hormones. The combination of BAP (0.5 mg/l) and NAA (0.2 mg/l) significantly improved the shoot proliferation rate, with the highest percentage (60.4%) and the greatest number of regenerated shoots (4.8±2.33) observed on MS medium.In contrast, plantlets cultured in both AM and WPM media displayed lower averages for shoot and root numbers and lengths compared to those grown on MS medium. The maximum number of roots (3.1±1.66) was obtained when MS medium was augmented with IBA at a concentration of 1.0 mg/l.Subsequently, the produced plantlets were gradually transferred to pots filled with a mixture of perlite and cocopeat in a 1:3 ratio and placed in a greenhouse for further growth.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"157 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135371823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Using Vitrification–Dehydration Method as a New Protocol for In-vitro Cryopreservation of Date Palm Shoot Tip Cultivars 玻璃化脱水法作为枣椰树茎尖品种体外低温保存的新方案
Plant Cell Biotechnology and Molecular Biology Pub Date : 2023-10-18 DOI: 10.56557/pcbmb/2023/v24i5-68418
Heba Allah A. Mohasseb
{"title":"Using Vitrification–Dehydration Method as a New Protocol for In-vitro Cryopreservation of Date Palm Shoot Tip Cultivars","authors":"Heba Allah A. Mohasseb","doi":"10.56557/pcbmb/2023/v24i5-68418","DOIUrl":"https://doi.org/10.56557/pcbmb/2023/v24i5-68418","url":null,"abstract":"This study was conducted to develop an in vitro method for the short-term conservation of the planting materials of date palm (Phoenix dactylifera). The conservation of the genetic resources of date palm species and germplasm represented by the cryopreservation of biological material, such as shoot tips of date palm dropped in LN (Liquid Nitrogen) at low temperatures, is a safe method. The main objective of this study was the collection and in vitro germplasm preservation of the date palm cultivars, and this study evaluated the in vitro preservation and genetic stability of date palm shoot tip explants using dimethyl sulfoxide (DMSO) in the medium for long-term storage. Shoot tip explants of about 2-3 cm in length were excised from in vitro cultures and were transferred to preservation media. The results showed that the highest rates of survival (80%) and recovery (75%) were observed with 1.2 M sucrose. To determine the effect of vitrification on freezing tolerances, cultures were exposed to a solution that dissolved the glaze for 60-80 minutes. The maximum survival rate obtained with exposed cultures was 85%. RAPD (randomly amplified polymorphic DNA) was used to explain the differences in the genetic characteristics of cryogenic tissue cultures and non-cryogenic tissues of date palm. Both were similar to the germinated date palm in the open field. Finally, the cryopreserved plants were able to adapt to free-living conditions after acclimatization, All aspects will contribute to the improvement of the currently available techniques for the in vitro germplasm conservation of date palms.","PeriodicalId":34999,"journal":{"name":"Plant Cell Biotechnology and Molecular Biology","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135888540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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