Direct and Indirect Organogenesis in Olive (Olea europaea L.)

Abstract

Olive explants included of pieces of young stems which were divided into nodal explants cultured on MS media supplemented with cytokinins and auxin. Callus induction and different forms of shooting and adventitious lateral buds regeneration were dominant results. Level of auxin IBA in all treatments was used at 0.1 mg/l. But cytokinins combination were used at different combinations. Combination of BA and ZEA led to more organogenesis and combination of BA and 2ip led to callus induction. Organogenesis was occurred with three different forms including indirect with passing callus phase, direct with growth of lateral buds and elimination of dominance apical and direct with regeneration of new lateral buds on explants.