针刺研究最新文献

{"title":"Effect of transcutaneous auricular vagus nerve stimulation combined with patient-controlled intravenous analgesia on postoperative pain in patients with total knee arthroplasty.","authors":"Hao-Xun Huang, Xing-He Wang, Yan Zhang, Jin Zhang, Teng Li, Zi-Heng Wang, Yang-Zi Zhu, Li-Wei Wang","doi":"10.13702/j.1000-0607.20231101","DOIUrl":"10.13702/j.1000-0607.20231101","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the effect of transcutaneous auricular vagus nerve stimulation (taVNS) combined with patient-controlled intravenous analgesia (PCIA) on postoperative pain in patients undergoing total knee arthroplasty (TKA).</p><p><strong>Methods: </strong>A total of 140 patients with TKA were randomly divided into the taVNS group (<i>n</i>=70, with 5 dropped-out) and the sham stimulation group (<i>n</i>=70, with 6 dropped-out). The taVNS group received taVNS at the left ear conchae combined with PCIA treatment, while the sham stimulation group received sham taVNS at the left ear conchae combined with PCIA treatment. The time-weighted average (TWA) pain score, visual analogue scale (VAS) score of 1-7 days after surgery, the occurrence of adverse reactions such as nausea, vomiting, skin pruritus, drowsiness, respiratory depression, and the number of PCIA pump compressions within 48 hours after surgery were observed and compared between the 2 groups. The Beck Anxiety Inventory (BAI) scores, Bake Depression Inventory (BDI) scores, and Pittsburgh Sleep Quality Index (PSQI) scores were obtained on the day before surgery and 7 days after surgery.</p><p><strong>Results: </strong>Compared with the sham stimulation group, the TWA pain score of the taVNS group was significantly decreased at the 7^th day postoperatively (<i>P</i><0.01), and the VAS scores of the taVNS group were significantly lower than those of the sham stimulation group on postoperative days 3^rd, 4^th, and 5^th (<i>P</i><0.001, <i>P</i><0.01, <i>P</i><0.05), with no statistical significance in VAS scores on postoperative days 1^st, 2^nd, 6^th, and 7^th. Compared with the sham stimulation group, the incidence of nausea and the number of PCIA pump compressions within 48 hours after surgery in the taVNS group was significantly decreased (<i>P</i><0.05). There was no statistical significance in the differences between the BDI, BAI, and PSQI scores before and after surgery in both groups. PSQI scores in the sham stimulation group and taVNS group were significantly lower than those in the same group 1 day before surgery (<i>P</i><0.01, <i>P</i><0.001).</p><p><strong>Conclusions: </strong>The taVNS combined with PCIA can reduce postoperative pain, the incidence of nausea, and the opioid dosage in TKA patients.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 2","pages":"190-196"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The key to acupuncture-moxibustion diagnosis and examination lies in the \"knowledge\" of both doctors and patients - revisiting the \"Yi Zhi Wei Shu, Yi Tong Wei Shu\".","authors":"Xin-Wei Li, Lu Liu, Wei You, Jia Wei, Jin-Lian Cheng, Bin Li, Shi-Yan Yan, Zi-Yi Li, Bing Shao, Fan Gao, Hui-Lin Liu","doi":"10.13702/j.1000-0607.20240807","DOIUrl":"10.13702/j.1000-0607.20240807","url":null,"abstract":"<p><p>The correct selection of the stimulated site is the key to the effectiveness of acupuncture and moxibustion. In the present paper, we propose that in the treatment of Jingjin (muscle, tendon, fascia, ligament, etc.) disease, the stimulated position of acupuncture or moxibustion should be determined by careful examination, rather than only selection of the fixed acupoints. The \"Yi Zhi Wei Shu\" (\"Zhi\"i.e. \"knowledge\"), means: the limit of the number of acupuncture or moxibustion treatment sessions is according to the therapeutic effect (improvement or cured) of a disease achieved, while the \"Yi Tong Wei Shu\" (\"\") refers to needling the site where the pain is, i.e. Ashi point, recorded in book <i>Lingshu. Jingjin</i> (<i>Miraculous Pivot: Aponeurotic Structure along Meridians</i>), are the basic principles and methods for the treatment of Jingjin disease. The ancient physicians and modern doctors have different understandings on the selection of number of treatment sessions, and the stimulation of Ashi-points. Combining the meaning of \"Zhi\" of \"Yi Zhi Wei Du\" (\"\"meaning that the herb-medicines can be taken until the clinical condition has been improved to a certain extent, and it is not necessary to stop the medicines until it is completely cured) in <i>Shang Han Za Bing Lun</i> (<i>Treatise on Febrile and Miscellaneous Diseases</i>) for the first time, we hold that the \"Zhi\" of \"Yi Zhi Wei Shu\" reflects the doctors' ability to grasp the rules of diagnosis and treatment of diseases, that is, doctors' professional \"cognition\". We also propose that the saying \"Yi Zhi Wei Shu, Yi Tong Wei Shu\" refers to guiding how to determine the stimulated spots of acupuncture or moxibustion for Jingjin disease. It is by no means to choose the pain site complained by the patient, but the doctor's judgement is according to the patient's perception of tenderness proposed during physical examination such as touching and pressing, which is the patient's \"Zhi\". The saying \"Yi Zhi Wei Shu, Yi Tong Wei Shu\" could be understood that the determination of the stimulated spots of acupuncture and moxibustion for Jingjin disease should be based on the doctor's professional \"cognition\". During the physical examination, the doctor makes a judgment on the patient's \"knowledge of pain site\", so as to determine the treatment points, that is, the \"Zhi\" of both doctors and patients should be achieved. Furthermore, combined with the theory of acupuncture and moxibustion in the treatment of Jingjin disease, tension-type headache, postherpetic neuralgia, primary trigeminal neuralgia and other diseases and heat-sensitive moxibustion theory, the connotation and clinical application value of \"Yi Zhi Wei Shu, Yi Tong Wei Shu\" are explained, in order to provide enlightenment for the determination of stimulated points of acupuncture and moxibustion.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 2","pages":"224-231"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of penetrative needling of \"Zhibian\" (BL54) through \"Shuidao\" (ST28) on expression of internal and external apoptosis-related factors in ovarian of mice with poor ovarian response.","authors":"Jia Ren, Bo-Ya Chang, Meng-Jie Cui, Zhen Gao, Yu-Xia Cao, Hai-Jun Wang","doi":"10.13702/j.1000-0607.20231108","DOIUrl":"10.13702/j.1000-0607.20231108","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of penetrative needling of \"Zhibian\" (BL54) through \"Shuidao\" (ST28) on the expressions of internal and external apoptosis-related factors in mice with poor ovarian response (POR), so as to explore its mechanisms underlying improvement of POR.</p><p><strong>Methods: </strong>Sixty-nine female C57BL/6N mice were randomly divided into blank, model and acupuncture groups (<i>n</i>=23 in each group). The POR model was established by gavage of tripterygium wilfordii polyglucoside suspension (50 mg·kg^-1). After successful modeling, the rats in the acupuncture group received penetrative needling of BL54 through ST28, with the needle retained for 20 min, once a day for 14 days. Thirty healthy male mice of the same strain aged 7-8 weeks were co-housed with female mice in a 1∶1 ratio. Vaginal exfoliative cell smears were used to observe the changes in the estrous cycle of female mice. The number of follicles were observed under light microscope, wet weight of ovaries was recorded and ovarian index was calculated. The pregnancy rate and number of embryos were observed. ELISA method was used to detect the low levels of anti Mullerian hormone (AMH), follicle stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2) contents in the serum of female mice. HE staining was used to observe the morphology of ovarian tissue. TUNEL staining was used to observe the apoptosis rate of ovarian cells. The mRNA and protein expression levels of cysteine-aspartic acid protease (Caspase)-3, Bcl-2-associated X protein (Bax), B-cell lymphoma-2 (Bcl-2), fas cell surface death receptor (Fas), and fas-associated death domain (FADD) in ovarian tissue were detected by real-time quantitive PCR and Western blot, respectively.</p><p><strong>Results: </strong>Compared with the blank group, the rate of estrous cycle disorder, the contents of FSH and LH in serum, the rate of ovarian apoptosis, the mRNA and protein expression levels of Caspase-3, Bax, Fas and FADD in ovarian tissues were significantly increased (<i>P</i><0.01); whereas the pregnancy rate, the number of embryos, the number of eggs obtained, ovarian wet weight, ovarian index, the contents of AMH and E2, the mRNA and protein expression levels of Bcl-2 were significantly decreased (<i>P</i><0.01) in the model group. Following the intervention, the above-mentioned indexes were all reversed in the acupuncture group (<i>P</i><0.01, <i>P</i><0.05).</p><p><strong>Conclusions: </strong>Penetrative needling of BL54 through ST28 can increase the ovarian response in POR mice, which may be associated with its function in regulating the expression of internal and external apoptosis-related factors in ovarian tissue.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 2","pages":"123-130"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect and mechanism of acupotomy tendon-sparing and knot-dissolving technique in inhibiting bone destruction in rats with rheumatoid arthritis based on the PI3K/AKT/NFATc1 pathway.","authors":"Hao-Lin Li, Ping Chen, Hai-Dong Wang, Wei-Qing Li, Fang-Mei Jin, Li-Li Kan, Fang-Hong Nian, Le-le Hu, Juan-Juan Yang, Luo-Yang Jing","doi":"10.13702/j.1000-0607.20240993","DOIUrl":"10.13702/j.1000-0607.20240993","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To observe the effect of acupotomy tendon-sparing and knot-dissolving technique on bone destruction and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/nuclear factor of activated T cells c1 (NFATc1) pathway in rats with rheumatoid arthritis (RA), so as to investigate the underlying mechanism.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Forty SD rats were randomly divided into normal, model, medication (tripterygium wilfordii), and acupotomy groups, with 10 rats in each group. Except for the normal group, the rest of the rats were injected with bovine type II collagen emulsion at the base of tails to establish a collagen-induced RA model. The acupotomy group was treated with acupotomy tendon-sparing and knot-dissolving technique, once every 3 days, with a continuous intervention for 9 times. The medication group was given tripterygium wilfordii polyglycoside suspension (8 mg·kg&lt;sup&gt;-1&lt;/sup&gt;) by gavage, once a day for 28 days continuously. The swelling degree of the ankle joint and the arthritis index score of the rats were observed. Micro-CT scanning was used to observe the degree of bone destruction in the left ankle joint. HE staining and ferruben-solid green staining were used to observe the pathological morphological changes of synovial and cartilage tissue respectively. ELISA was used to detect the contents of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and IL-17 in synovial tissue. Tartrate-resistant acid phosphatase (TRAP) staining was used to observe the number of osteoclasts in the left ankle joint. Western blot was used to detect the expression levels of NFATc1, p-PI3K and p-AKT proteins in synovial tissue. Immunohistochemistry was used to detect the positive expressions of matrix metalloproteinase-9 (MMP9), cathepsin K (CTSK) and TNF receptor-associated factor 6 (TRAF6) in the synovial tissue of the ankle joint.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;In comparison with the normal group, the bones of the ankle joint and toes of rats were severely eroded, with an uneven surface in the model group; there was a large number of inflammatory cell infiltrations in the synovial tissue, obvious damage to the articular cartilage, and disordered arrangement of synovial cells; the cartilage matrix was damaged, the cartilage layer was rough, and the subchondral bone structure was disordered. In comparison with the model group, the above histopathological changes in the medication group and the acupotomy group were alleviated. Compared with the normal group, the joint swelling degree, arthritis index, the ratio of bone surface area to bone volume (BS/BV), trabecular number (Tb.N), trabecular separation (Tb.Sp), the contents of TNF-α, IL-1β, IL-6 and IL-17 in synovial tissue, the number of osteoclasts in the joint, the expressions or ratios of NFATc1, p-PI3K/PI3K, p-AKT/AKT proteins in synovial tissue, and the positive expressions of TRAF6, CTSK and MMP9 proteins in synovial tissue in the model group were signifi","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 2","pages":"131-140"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical observation on treatment of patients with kidney-<i>yang</i> deficiency type ankylosing spondylitis by using Bushen Tongdu external treatment.","authors":"Xing-Yu Zhou, Xin-Yi Wang, Yu-Fei Zhang, Xiao-Long Wang, Xiu-Xiu Zhang, Yuan-Yuan Liu, Peng-Yan Li, Yuan-Sheng Tian","doi":"10.13702/j.1000-0607.20231040","DOIUrl":"10.13702/j.1000-0607.20231040","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To observe and evaluate the effectiveness and safety of Bushen Tongdu (reinforcing the kidney-&lt;i&gt;yang&lt;/i&gt; and regulating the Governor Vessel) external treatment for patients with kidney-&lt;i&gt;yang&lt;/i&gt; deficiency type ankylosing spondylitis (AS).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Patients with Kidney-&lt;i&gt;yang&lt;/i&gt; deficiency type AS were randomly divided into an observation group(36 cases, 4 cases dropped out) and a control group(36 cases, 3 cases dropped out). The observation group were received the Bushen Tongdu external treatment which was a combination of Ren Du Zhou-Tian moxibustion and acupoint embedding therapy, i.e. performing ginger-juice soaked nonwoven separated moxibustion first at the back covering the region from Dazhui (GV14) to Yaoshu (GV2) for 1 h, and then at the thoracoabdominal region covering the part from Tiantu (CV22) to Zhongji (CV3) for 1 h, followed by performing acupoint catgut embedding at one or two pairs of GV14, Shenshu (BL23), Dachangshu (BL25), Xuehai (SP10), and Zusanli (ST36), once a week, for 8 weeks. The control group received oral administration of salazosulfapyridine capsules (0.2 g/time), once a day for 8 weeks. Changes of the patients' conditions were evaluated by using traditional Chinese medicine (TCM) syndrome score (0 to 3 points for cold and painful lower back, morning stiffness, limited lumbar movement, soreness and weakness of waist and knees, etc.) and the total score (0 to 18 points), the Bath ankylosing spondylitis disease activity index (BASDAI) score (0 to 10 points for fatigue, physical discomfort caused by pain, swelling, or tenderness in the spine and peripheral joints, duration and severity of morning stiffness, etc). The therapeutic effect was assessed . The safety observation included fainting during acupuncture or moxibustion treatment, burn, etc. The contents of serum interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and matrix metalloproteinase-3 (MMP-3) were detected before and after the treatment.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;After 8 weeks of treatment, the TCM syndrome score, total score, BASDA score, contents of serum IL-6, TNF-α and MMP-3 were strikingly decreased in both groups compared with those of their own pre-treatment (&lt;i&gt;P&lt;/i&gt;&lt;0.05), and the therapeutic effect of the observation group was significantly superior to that of the control group in lowering the scores of cold and painful lower back, weakness in the lower back and knees, preference for warmth and aversion to cold, pain worsened by cold, and total score, BASDA score, and contents of serum IL-6, TNF-α and MMP-3 (&lt;i&gt;P&lt;/i&gt;&lt;0.05). The total effective rate of observation group was 90.63% (29/32), which was higher (&lt;i&gt;P&lt;/i&gt;&lt;0.05) than that of control group (75.76%, 25/33).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;The Bushen Tongdu external treatment method significantly improves AS patients' TCM clinical symptoms, alleviates inflammatory reactions, and demonstrates a significant efficacy w","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 2","pages":"183-189"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cerebral protective effect of acupuncture based on its regulative effect on programmed cell death.","authors":"Gong-Ming Yuan, Feng-Ya Su, Pei-Yun Li, Yong-Ming Guo, Jian Zhang, Yuan Xu, Zhong-Xi Lü, Ji-Yu Zhao, Yi-Nan Gong","doi":"10.13702/j.1000-0607.20230934","DOIUrl":"10.13702/j.1000-0607.20230934","url":null,"abstract":"<p><p>Programmed cell death (PCD) in the brain occurs throughout the whole process of life, and abnormal PCD may be an important factor leading to encephalopathy. It has been demonstrated that acupuncture has a good cerebral protective effect in the treatment of epilepsy, Alzheimer's disease, Parkinson's disease, depression, anxiety, insomnia, migraine and other dozens of brain diseases, and possesses characteristics of multi-point, networking, two-way and holistic regulation. In the present paper, we sum up the protective effect of acupuncture therapy from 1) cell apoptosis (for example, improving post-surgery cognition impairment, traumatic brain injury and ischemic stroke by relieving mitochondrial damage, activating mitochondrial autophagy, increasing blood-brain barrier permeability, and thus reducing cell apoptosis ), 2) cell autophagy (for example, improving post-surgery cognition impairment, traumatic brain injury and ischemic stroke by relieving mitochondrial damage, activating mitochondrial autophagy, and increasing blood-brain barrier permeability, and thus reducing cell apoptosis), 3) pyroptosis (for example, inhibiting abnormally-activated inflammasomes, and caspase and gasdermin D activity to remit pyroptosis in the treatment of stroke, depression, Parkinson's disease, and Alzheimer's disease animal models), 4) ferroptosis (for instance, regulating iron ion metabolism, relieving lipid peroxidation damage, and thus playing a brain protection role in the treatment of ischemic and hemorrhagic stroke animal models), 5) necroptosis (inhibiting necrotic apoptosis mediated by receptor-interacting protein kinase 1 in intracerebral hemorrhage rats), and so on. We believe that along with the continuous development of brain science, artificial intelligence, big data and other new concepts and new technologies, it is expected to expand and improve the research level and quality of acupuncture in the prevention and treatment of encephalopathy through faster, more accurate and more comprehensive methods, which will help promote the modern interpretation of the brain protective effect of traditional acupuncture from the perspective of acupuncture regulation of PCD.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 2","pages":"210-216"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acupuncture inhibits epithelial mesenchymal transition in allergic asthma rats by activating the PI3K/AKT pathway through DEK activation.","authors":"Xin-Yun Miao, Yu-E Lü, Mei-Qi Ji, Hao Yin, Han Wu, Zhi-Xiang Hu, Chun-Tao Zhai, Lei Li","doi":"10.13702/j.1000-0607.20230951","DOIUrl":"10.13702/j.1000-0607.20230951","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To observe the effect of acupuncture at \"Dazhui\" (GV14) and \"Shenzhu\" (GV12) on DEK/phosphorylated phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) pathway and epithelial-mesenchymal transition related factors in rats with allergic asthma (AA), so as to explore its potential mechanism in the treatment of AA.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Sixty SD rats were randomly divided into normal, model, acupuncture, dexamethasone (DEX) and sham-acupuncture groups, with 12 rats in each group. The AA model was made by intraperitoneal injection of mixture solution of ovalbumin and aluminium hydroxide gel (on day 1 and 7) and followed by inhalation of atomized 1% ovalbumin (30 min, from day 15, once daily for 14 days). Rats in the acupuncture group received acupuncture at GV14 and GV12, while rats in the sham-acupuncture group received acupuncture at the non-meridian and non-acupoint point, both for 20 min, once daily for 14 days. Rats of the DEX group received intraperitoneal injection of DEX (0.5 mg/kg), once daily for 14 days. H.E. staining was used to observe the changes of pathological morphology in lung tissue. ELISA was used to detect the levels of interleukin (IL)-4 and IL-5 in broncho alveolar lavage fluid (BALF), and serum immunoglobulin IgE and IgM. Western blot was used to detect the expressions of DEK, PI3K, AKT, phosphorylated (p)- PI3K, p-AKT, and E-cadherin in lung tissue. Immunofluorescence staining was used to detect the positive expressions of DEK, p-PI3K, p-AKT, and E-cadherin in lung tissue.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Compared with the normal group, the levels of IL-4, IL-5 in BALF and serum IgE and IgM, the protein expression levels of DEK, p-PI3K and p-AKT in lung tissue, the positive expression levels of DEK, p-PI3K and p-AKT were increased in the model group (&lt;i&gt;P&lt;/i&gt;&lt;0.01), while the protein expression and positive expression levels of E-cadherin were decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.01). In comparison with the model group, the contents of IL-4, IL-5 in BALF and serum IgE and IgM, the protein expressions of DEK, p-PI3K and p-AKT, the positive expression levels of DEK and p-PI3K in the acupuncture and DEX groups were decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.01, &lt;i&gt;P&lt;/i&gt;&lt;0.05), while the positive expression of E-cadherin was increased (&lt;i&gt;P&lt;/i&gt;&lt;0.05). The positive expression level of p-AKT in the acupuncture group was decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.05). The level of IL-5 in BALF was decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.01) in the sham-acupuncture grourp relative to the model group. H.E. staining showed that the rats in the model and sham-acupuncture groups exhibited thickened bronchial walls and increased infiltration of inflammatory cells, while these situations were obviously milder in both acupuncture and DEX groups.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;Acupuncture can improve the airway remodeling of AA rats induced by ovalbumin, which may be associated with its functions in down-regulating the expression of DEK, inhibiting the activation","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 2","pages":"174-182"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on the mechanisms of acupuncture in improving ischemic stroke through autophagy.","authors":"Yu Wang, Yi-Ning Dai, Xiao-Jing Guo, Miao Hua, Li Zhang","doi":"10.13702/j.1000-0607.20230791","DOIUrl":"10.13702/j.1000-0607.20230791","url":null,"abstract":"<p><p>Stroke is a major disease with high incidence, disability rate, and mortality worldwide. In recent years, a large number of studies have comfirmed that acupuncture treatment for ischemic stroke is closely related to the regulation of autophagy mechanisms. This article summarizes the literature in recent years, discusses the effects of acupuncture on the degradation pathways mediated by autophagosomes, the expression of autophagy-related proteins, autophagy-related pathways, and the impact of mitophagy, and further explores the mechanisms of acupuncture in regulating autophagy to improve ischemic stroke, providing new ideas and schemes for clinical treatment.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 2","pages":"204-209"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical observation on the efficacy of ringheaded thumb-tack needle therapy combined with tuina and active functional exercise in the treatment of neck-type cervical spondylosis.","authors":"Zhi-Long Zhang, Tian-Xin Chen, Yu-Jia Zhanghan, Tian-Xiang Xin, Yu-Qi Zhu, Sheng-Ping Yang","doi":"10.13702/j.1000-0607.20230798","DOIUrl":"10.13702/j.1000-0607.20230798","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the clinical efficacy of acupressure therapy combined with Tuina and active functional exercise in the treatment of neck-type cervical spondylosis.</p><p><strong>Methods: </strong>Sixty patients with cervical spondylosis were randomly divided into a control group and an experimental group, with 30 cases in each group. The control group was treated with Tuina and active functional exercise. Tuina therapy lasted 10-15 min per session, twice a week for 4 weeks. Functional exercises were performed once in the morning and once in the evening, each session lasting about 10 min, for a total of 4 weeks. The experimental group received the same treatment as the control group combined with ringheaded thumb-tack needle therapy which was applied to 5 Ashi points, with needle retention for 3 days followed by 1 day of rest, for a total of 4 weeks. The neck disability index (NDI) and the short-form of McGill pain questionnaire (SF-MPQ) including visual analog scale (VAS), present pain intensity (PPI) and pain rating index (PRI) were used before treatment, after 2 weeks and after 4 weeks of treatment. The modified Macnab efficacy evaluation criteria were used to assess treatment outcomes, and safety was also evaluated.</p><p><strong>Results: </strong>Compared with baseline, both groups showed significant reductions in NDI, VAS, PPI, and PRI scores after 2 and 4 weeks of treatment (<i>P</i><0.01). The improvement in the experimental group was significantly greater than that in the control group (<i>P</i><0.05). The clinical efficacy rate (excellent and good) was 60.0% (18/30) in the experimental group, compared to 30.0% (9/30) in the control group, with the experimental group showing better clinical outcomes (<i>P</i>< 0.05). No adverse reactions were reported during the study.</p><p><strong>Conclusions: </strong>Acupressure therapy combined with Tuina and active functional exercise can effectively improve symptoms such as neck dysfunction and pain in patients with cervical spondylosis with neck syndrome. The addition of acupressure therapy significantly enhances the clinical efficacy of Tuina and functional exercise, with confirmed therapeutic benefits and good safety.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 2","pages":"197-203"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of electroacupuncture on regulation of the basic fibroblast growth factor/glycogen synthase kinase-3β pathway in Parkinson's disease mice.","authors":"Yuan-Yuan Li, Xiao-Li Bao, Yao Wang, Yan-Chun Wang","doi":"10.13702/j.1000-0607.20230972","DOIUrl":"10.13702/j.1000-0607.20230972","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of electroacupuncture (EA) on basic fibroblast growth factor (BFGF)/glycogen synthase kinase-3β (GSK-3β) pathway in Parkinson's disease (PD) mice, so as to explore the potential mechanisms of EA in treating PD.</p><p><strong>Methods: </strong>Fifty C57/BL6 male mice were randomly divided into the control, model, EA, inhibitor and EA+inhibitor (combination) groups, with 10 mice in each group. The PD mouse model was established by intraperitoneal injection of 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine. Mice in the EA group received EA stimulation at \"Fengfu\" (GV16), \"Taichong\"(LR3) and \"Zusanli\"(ST36) for 30 min, once daily for 12 days. The inhibitor group received gavage of fibroblast growth factor receptor (FGFR) inhibitor AZD4547 (12.5 mg·kg^-1·d^-1) for 12 consecutive days. The open box experiment was used to observe the autonomous movement ability of mice. The expression level of tyrosine hydroxylase (TH) in substantia nigra was detected by immunohistochemistry. The apoptosis rate of substantia nigra cells was measured by TUNEL method. The relative protein expression levels of BFGF, phosphorylated phosphatidylinositol 3-kinase (p-PI3K), phosphorylated protein kinase B (p-AKT), p-GSK-3β, B-lymphomatoma-2 gene (Bcl-2), and Bcl associated X protein (Bax) in substantia nigra were detected by Western blot.</p><p><strong>Results: </strong>After modeling, compared with the control group, the total movement distance was shorten and the duration of rest was prolonged in the open box experiment (<i>P</i><0.01), the average absorbance of TH in the substantia nigra of the midbrain was decreased (<i>P</i><0.01), and the apoptosis rate of substantia nigra cells in the midbrain was increased (<i>P</i><0.01), the protein expression levels of BFGF, p-PI3K, p-AKT, p-GSK-3β and Bcl-2 were decreased (<i>P</i><0.01), while the expression level of Bax protein was increased (<i>P</i><0.01) in the model group. Compared with the model and the combination groups, the total movement distance was prolonged and the duration of rest was shorten (<i>P</i><0.01), the average absorbance of TH was increased (<i>P</i><0.05), and the apoptosis rate was decreased (<i>P</i><0.01, <i>P</i><0.05), the expression levels of BFGF, p-PI3K, p-AKT, p-GSK-3β and Bcl-2 were increased (<i>P</i><0.01, <i>P</i><0.05), while the expression level of Bax was decreased (<i>P</i><0.05) in the EA group.</p><p><strong>Conclusions: </strong>EA at GV16, LR3 and ST36 can alleviate motor disorders, reduce cell apoptosis, protect dopaminergic neurons in mice with PD, which may be related to its effect in regulating the BFGF/GSK-3β pathway.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 2","pages":"167-173"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}