Journal of Chromatography B最新文献

{"title":"Quantification of carbohydrates in sugar beet roots by using ultra-fast liquid chromatography coupled with time of flight mass spectrometry","authors":"Anuradha Singh ,&nbsp;Andrew Thompson ,&nbsp;Shyam L. Kandel ,&nbsp;Rajtilak Majumdar ,&nbsp;Colleen Pfaff","doi":"10.1016/j.jchromb.2025.124783","DOIUrl":"10.1016/j.jchromb.2025.124783","url":null,"abstract":"<div><div>The quantification of sucrose and other carbohydrates in sugar beet roots is essential prior to their processing to assess sugar production yield. In this study, a rapid, highly sensitive and selective ultra-fast liquid chromatography coupled with time of flight mass spectrometry (UFLC-ToFMS) method was developed and validated for the simultaneous analysis of monosaccharides (fructose, glucose-galactose), a disaccharide (sucrose), and a trisaccharide (raffinose). The method showed 1000-fold higher sensitivity, with LOD and LOQ ranging between 0.03–0.23 μg/mL and 0.09–0.7 μg/mL, compared to the standard HPLC-RI method. Additionally, the method was successfully applied to determine carbohydrate levels in sugar beet roots.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1267 ","pages":"Article 124783"},"PeriodicalIF":2.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144997302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic effects of Panax ginseng polysaccharides on qi deficiency diabetes: Insights from microbiomics and metabolomics","authors":"Yang Gao ,&nbsp;Yi Wu ,&nbsp;Meiyuan Wang ,&nbsp;Shu Liu ,&nbsp;Zhongying Liu","doi":"10.1016/j.jchromb.2025.124781","DOIUrl":"10.1016/j.jchromb.2025.124781","url":null,"abstract":"<div><div><em>Panax ginseng</em>, a traditional medicinal and edible plant valued for its tonifying properties, exhibits diverse pharmacological activities, including anti-fatigue, anti-oxidation and anti-diabetes. Polysaccharides represent a key active ingredient of <em>Panax ginseng</em>, known for their immunomodulatory properties and potential for development as health foods. However, the effect and mechanism of <em>Panax ginseng</em> polysaccharides (GP) on improving qi deficiency diabetes (QDD) is still unclear. In this study, a multi-omics approach integrating microbiomics, untargeted and targeted metabolomics was used to elucidate the protective effect and mechanisms of GP based on the QDD rat model. Furthermore, the prebiotic effects of GP were explored through microbiome and metabolome profiling. GP demonstrated significant therapeutic efficacy in QDD rats, manifested by the amelioration of hyperlipidemia and insulin resistance, reduction of oxidative stress, enhancement of immune responses, and restoration of intestinal injury. Untargeted fecal metabolomics and 16S rRNA gene sequencing revealed that GP significantly altered the abundance of 31 metabolites and corrected gut microbiota dysbiosis in QDD rats. Additionally, GP ameliorated bile acid metabolism disorders, significantly increased short-chain fatty acids (SCFAs) levels, and reduced branched-chain amino acids (BCAAs) concentrations. Spearman correlation analysis further confirmed significant associations between gut microbes and metabolites. Thus, GP exerts its therapeutic effect on QDD by ameliorating intestinal flora imbalance and associated metabolic disorders involving bile acids, branched-chain amino acids, and short-chain fatty acids. Together, these findings provide a foundation for the further development of GP-based functional foods.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1267 ","pages":"Article 124781"},"PeriodicalIF":2.8,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a validated and sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to simultaneous determination of crizotinib, alectinib and lorlatinib in human plasma","authors":"Haohua Chi ,&nbsp;Shu Li ,&nbsp;Fang Liu ,&nbsp;Bo Li ,&nbsp;Wenqian Chen ,&nbsp;Cheng Zhang ,&nbsp;Wei Zhou ,&nbsp;Meng Yang ,&nbsp;Pengmei Li","doi":"10.1016/j.jchromb.2025.124770","DOIUrl":"10.1016/j.jchromb.2025.124770","url":null,"abstract":"<div><div>Crizotinib, alectinib, and lorlatinib are tyrosine kinase inhibitors used sequentially in non-small cell lung cancer (NSCLC) therapy. Therapeutic drug monitoring (TDM) is valuable during sequential treatment, enabling simultaneous quantification. Consequently, an analytical method capable of multiplexing these three compounds into a single assay is highly applicable for routine TDM. A multiplexed UHPLC-MS/MS method quantifying all three drugs was validated for selectivity, matrix effects, linearity (20–1000 ng/mL), accuracy, precision, carryover, and stability. Matrix effects were negligible in both plasma from healthy volunteers and plasma from patients with hyperlipidemia. No significant interfering responses were observed for crizotinib, alectinib, lorlatinib, or their corresponding isotope-labeled internal standards. Sample stability was confirmed under ambient conditions (25 °C) for at least 24 h. Furthermore, the pretreated supernatant remained stable in the auto sampler (15 °C) for 24 h. This validated method is suitable for application in pharmacokinetic studies and exposure-response assessments.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1267 ","pages":"Article 124770"},"PeriodicalIF":2.8,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LC-MS/MS method for quantifying the HIV-1 broadly neutralizing antibody PGT 121.414.LS in human serum","authors":"Connor E. Gould ,&nbsp;Qing Ma ,&nbsp;Raymond Cha ,&nbsp;Kevin J. Zemaitis ,&nbsp;Robin DiFrancesco ,&nbsp;Gene D. Morse ,&nbsp;Troy D. Wood","doi":"10.1016/j.jchromb.2025.124774","DOIUrl":"10.1016/j.jchromb.2025.124774","url":null,"abstract":"<div><div>Quantitation of human immunodeficiency virus-1 (HIV-1) broadly neutralizing antibodies (bNAbs) in human serum is required for clinical trials investigating the pharmacokinetics, pharmacodynamics, and drug interactions of these treatments. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is gaining interest as an alternative to ligand binding for therapeutic antibody quantitation in serum. We report the validation of a method using nonspecific purification and targeted LC-MS/MS to quantify PGT 121.414.LS (a bNAb in development for HIV-1 prevention and treatment) in human serum. High-resolution spectra of tryptic peptides derived from the variable region were obtained on an Orbitrap for surrogate peptide selection, followed by multiple reaction monitoring using triple quadrupole mass spectrometry. Surrogate peptides were evaluated for linearity and reproducibility across the therapeutic concentration range using immunopurification or ammonium sulfate precipitation. Using ammonium sulfate precipitation, linear calibration curves were validated over 10–500 μg/mL (LLOQ at 10 μg/mL) using stable isotope labeled peptide internal standards. Method accuracy and reproducibility were evaluated using quality control samples (QCs) at four concentrations in the linear range. The average concentrations of all QCs fell within ICH M10 acceptance criteria. Matrix effects were investigated at the low and high QC concentrations across six lots of human serum. Dilutional integrity, stability, and effects of hemolysis were also assessed. The method exhibits minimal carryover and negligible crosstalk. The assay provides accurate quantification of PGT 121.414.LS in serum over the range of concentrations anticipated in specimens from treated persons living with HIV (PLWH) after initial dosing and prior to subsequent dosing of PGT 121.414.LS.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1266 ","pages":"Article 124774"},"PeriodicalIF":2.8,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144916975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a LC–MS/MS method for the detection of 38 benzodiazepines and 2 Z-drugs in blood","authors":"Yu Liu ,&nbsp;Xin-ze Liu ,&nbsp;Zhen-shuo Guo ,&nbsp;Ping Xiang ,&nbsp;Hui Yan","doi":"10.1016/j.jchromb.2025.124772","DOIUrl":"10.1016/j.jchromb.2025.124772","url":null,"abstract":"<div><div>Benzodiazepines and <em>Z</em>-drugs are commonly used as prescription medications to treat anxiety, epilepsy, insomnia, and alcohol withdrawal syndrome, but their use can lead to tolerance, dependence, and withdrawal reactions if taken against official guidelines. Furthermore, designer benzodiazepines, most of which lack clinical and toxicological data, have entered the illicit drug market as new psychoactive substances and are used for recreational purposes. Their abuse can cause confusion, memory loss, respiratory depression, and even death, especially when combined with other sedative-hypnotics or alcohol. Therefore, new qualitative and quantitative methods for benzodiazepines and <em>Z</em>-drugs are needed for use in forensic and clinical toxicology. This study explored the sample preparation and liquid-liquid extraction using 0.5 mL blood samples and 2 mL of extraction solvent for analysis of these drugs by liquid chromatography-tandem mass spectrometry and multiple reaction monitoring. The benzodiazepines were separated on a pentafluorophenylpropyl (PFPP) column using a mobile phase gradient consisting of A (water, 0.1 % formic acid, 5 % acetonitrile, and 20 mmol/L ammonium acetate) and B (acetonitrile) for 9 min. Validation steps confirmed that the method demonstrated good selectivity, sensitivity (limit of detection: 0.2 ng/mL, lower limit of quantification: 0.5 ng/mL), linearity (R² ≥ 0.99), accuracy, and precision (&lt;20 %). Matrix effects ranged from 35 to 126 %, and recoveries ranged from 17 to 99 %, with 35 compounds having recoveries of more than 50 %. The method was successfully applied for the identification and quantification of benzodiazepines and nonbenzodiazepines in blood samples from 15 authentic poisoning cases. Ten analytes were detected: alprazolam (130.2–575.3 ng/mL), hydroxyalprazolam (2.3–37.3 ng/mL), clonazepam (11.7–773.2 ng/mL), lorazepam (63.4–166.9 ng/mL), 7-aminoclonazepam (17.4–385.3 ng/mL), oxazepam (2.6–964.1 ng/mL), diazepam (227.2 ng/mL), nordazepam(22.4 ng/mL), zolpidem (11.8–64.0 ng/mL), midazolam (70.2 ng/mL), and hydroxymidazolam (162.8 ng/mL).</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1266 ","pages":"Article 124772"},"PeriodicalIF":2.8,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144922508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An automated Immunoaffinity liquid chromatography-tandem mass spectrometry assay for quantification of aldosterone in human plasma","authors":"Liang-Xing Li ,&nbsp;Jian-You Xue ,&nbsp;Wan-Ying Lin ,&nbsp;Xiao-Feng Yin ,&nbsp;Ting-Ting Luo ,&nbsp;Zhi-Liang Cai ,&nbsp;Chao-Chao Wu ,&nbsp;Qiang Gao ,&nbsp;Xin Li ,&nbsp;Ru-Yi Zhang","doi":"10.1016/j.jchromb.2025.124773","DOIUrl":"10.1016/j.jchromb.2025.124773","url":null,"abstract":"<div><div>The diagnosis of primary aldosteronism (PA) relies on the accurate determination of aldosterone. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has long been considered the gold standard for aldosterone quantification but it is hindered by labor-intensive sample preparation. To address this, we developed an immunoaffinity-mass spectrometry (iMS) assay on a fully automated device combining anti-aldosterone antibodies with stable isotope-labeled internal standards (IS). This method completes sample preparation within 15 min for at least six parallel samples in parallel with minimal manual intervention.</div><div>The key performance metrics include a lower limit of quantitation (LOQ) of 50 pg/mL, recovery rates between 105.1 and 113.9 %, and linearity in the range of 50–2000 pg/mL (R² = 0.9993). Inter-assay coefficient of variation (CV) ranged from 2.33 % to 3.91 %. In addition, plasma aldosterone concentrations by iMS and immunoassay had a high correlation coefficient (<em>R</em> = 0.947).</div><div>Overall, this automated high-throughput platform delivers clinical-grade sensitivity, precision, and scalability, making it suitable for routine testing and adaptable for other clinical analytes.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1266 ","pages":"Article 124773"},"PeriodicalIF":2.8,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144912164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a pseudotargeted metabolomics approach for relative quantification of free fatty acid double-bond isomers in beagle plasma","authors":"Wen Xiao ,&nbsp;Haihui Ren ,&nbsp;Yueyi Zhang ,&nbsp;Jiameng Han ,&nbsp;Qiaorong Liu ,&nbsp;Zunjian Zhang ,&nbsp;Yuan Tian","doi":"10.1016/j.jchromb.2025.124765","DOIUrl":"10.1016/j.jchromb.2025.124765","url":null,"abstract":"<div><div>Free fatty acids (FFAs) play a key role in living organisms and participate in metabolic processes, mainly as a source of energy for cells. The C<img>C position isomer of FFAs is strongly associated with many diseases, but quantification of its double bond position isomer remains a challenge in lipid analysis. In this study, based on the requirements of the pseudotargeted metabolomics, an analytical method based on a two-step derivatization LC-MS/MS method and a multiple reaction monitoring (MRM) mode was established and fully validated in terms of linearity, precision, and stability. The results demonstrated that the method relatively quantified 30 FFAs in plasma samples. These included 8 saturated fatty acids (SFAs), 12 monounsaturated fatty acids (MUFAs), and 10 polyunsaturated fatty acids (PUFAs). The developed method was applied to analyze the increase or down-regulation of the relative content of FFAs before and after the administration of the drug in beagles which may help to explain the therapeutic effect of sacubitril valsartan. The establishment of this method allows the study of FFAs in beagles plasma to be no longer limited to analysis at the subclass level but can be focused at the isomer level.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1266 ","pages":"Article 124765"},"PeriodicalIF":2.8,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144912163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous separation on a bisphenylureido β-cyclodextrin column and determination of eight antihistamine enantiomers in human plasma by HPLC","authors":"Jing Lin,&nbsp;Dan Li,&nbsp;Peiyuan Luo,&nbsp;Laisheng Li","doi":"10.1016/j.jchromb.2025.124771","DOIUrl":"10.1016/j.jchromb.2025.124771","url":null,"abstract":"<div><div>A <em>bis</em>(3,5-dichlorophenylureido)-β-cyclodextrin stationary phase (CUCDP) was prepared and characterized. It had strong chiral separation abilities for antihistamines (<em>R</em>s = 1.61–2.58) in isocratic elution, including chlorpheniramine maleate, bromopheniramine maleate, promethazine hydrochloride and trimeprazine tartrate. Based on CUCDP, a new HPLC method for simultaneous separation and determination of the above eight antihistamine enantiomers (<em>R</em>s = 1.58–1.92) in human plasma by gradient elution was established. The optimized conditions were as follows: a simple sample pretreatment with acetonitrile (ACN) to precipitate protein, a gradient elution of 0.5 % triethylammonium acetate (TEAA, pH = 4.0)-ACN as the mobile phase at a flow rate of 0.5 mL/min, column temperature at 20 °C, a photo-diode array detector (PDA) at 260 nm, and the sampling volume of 10 μL. The good linear relationships for all enantiomers were observed in the concetration range of 0.25–10.0 μg/mL (<em>R</em>² = 0.9986–0.9993). The average recoveries were 84.80 %–103.30 % with the RSDs of 1.24 %–2.26 % (<em>n</em> = 5). The limits of detection (LODs) and limits of quantification (LOQs) were 0.015–0.05 μg/mL and 0.05–0.15 μg/mL, respectively. At present, the reported cyclodextrin-based CSPs had not such separation ability for antihistamines by HPLC. However, the new CUCDP exhibited high chiral selectivity for antihistamines, which was mainly related to the introduction of <em>bis</em>phenylureido group into the rim of cyclodextrin, and the strengthening of hydrogen bonding, π–π stacking and inclusion between CUCDP and analytes. This method could save analysis time, reduce solvent consumption, and improve efficiency at low cost.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1266 ","pages":"Article 124771"},"PeriodicalIF":2.8,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144892279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel ionic liquid 3-(2-hydrazinyl-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride as a derivatization reagent for HPLC-HRMS determination of steroid hormones in urine","authors":"M. Zorina ,&nbsp;Y.-Q. Feng ,&nbsp;Sanka N. Atapattu ,&nbsp;S. Girel ,&nbsp;Dzh. Konshina ,&nbsp;V.V. Konshin ,&nbsp;A. Temerdashev","doi":"10.1016/j.jchromb.2025.124760","DOIUrl":"10.1016/j.jchromb.2025.124760","url":null,"abstract":"<div><div>This work demonstrates for the first time the possibility of using a novel ionic liquid 3-(2-hydrazinyl-2-oxoethyl)-1-methyl-1H-imidazol-3-ium chloride ([HOMI]Cl) as a derivatization reagent for steroid hormones and their subsequent analysis using ultra-high performance liquid chromatography–quadrupole time-of-flight mass spectrometry in human urine. Derivatization conditions, such as pH, reagent concentration, reaction time and temperature were evaluated and optimized. A comparative study of [HOMI]Cl and hydroxylamine effectiveness revealed a formation of both <em>syn</em>- and anti-forms of the steroid derivatives for the novel [HOMI]Cl reagent similarly to a derivatization with hydroxylamine. Hence obtained limits of quantitation were comparable to the literature values obtained with Girard P and hydroxylamine reagents (0.15 to 7.5 ng/mL). Finally, in-depth optimization of [HOMI]Cl hydrophobicity and ionization source conditions were found necessary to further improve the electrospray response of hence derivatized steroids.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1265 ","pages":"Article 124760"},"PeriodicalIF":2.8,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144885559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of compounds in ginseng that activate CYP19A1 enzyme promoters for the treatment of postmenopausal osteoporosis using molecular docking and affinity ultrafiltration technology","authors":"Yiying Tan ,&nbsp;Yajing Li ,&nbsp;Haiqing Luo ,&nbsp;Yishan Li ,&nbsp;Lu Han ,&nbsp;Jiaming Shen ,&nbsp;Xuesheng Hu ,&nbsp;Xiaochen Gao ,&nbsp;Chunnan Li ,&nbsp;Jiaming Sun","doi":"10.1016/j.jchromb.2025.124767","DOIUrl":"10.1016/j.jchromb.2025.124767","url":null,"abstract":"<div><div>The primary etiology of postmenopausal osteoporosis is estrogen deficiency. The enzyme aromatase (CYP19A1) serves as the key rate-limiting enzyme in the conversion of androgens to estrogens. In this study, ginseng was selected as the subject of investigation. The study identified that the 50 % ethanol extract of ginseng exhibited the most potent activity in the determination of CYP19A1 enzyme activity. This extract was subsequently analyzed using UHPLC-QE Orbitrap-MS in conjunction with mass spectrometry molecular network technology. To identify the active components effective against postmenopausal osteoporosis, enzyme ultrafiltration affinity, molecular docking, and kinetic simulation techniques were employed. Cross-analysis of binding energy and affinity rate results revealed that ginsenoside Re and ginsenoside Rf possessed the highest absolute binding energy and affinity values, establishing them as the most effective active components. The mechanisms involving ALP, OPG, apoptosis, and qPCR were validated in vitro to confirm the anti-PMOP effects of these active ingredients. This study offers an efficient and rapid method for screening natural products to identify active components for the treatment of postmenopausal osteoporosis.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1265 ","pages":"Article 124767"},"PeriodicalIF":2.8,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}