Quantification of carbohydrates in sugar beet roots by using ultra-fast liquid chromatography coupled with time of flight mass spectrometry

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B Pub Date : 2025-09-01 DOI:10.1016/j.jchromb.2025.124783

Anuradha Singh , Andrew Thompson , Shyam L. Kandel , Rajtilak Majumdar , Colleen Pfaff

引用次数: 0

Abstract

The quantification of sucrose and other carbohydrates in sugar beet roots is essential prior to their processing to assess sugar production yield. In this study, a rapid, highly sensitive and selective ultra-fast liquid chromatography coupled with time of flight mass spectrometry (UFLC-ToFMS) method was developed and validated for the simultaneous analysis of monosaccharides (fructose, glucose-galactose), a disaccharide (sucrose), and a trisaccharide (raffinose). The method showed 1000-fold higher sensitivity, with LOD and LOQ ranging between 0.03–0.23 μg/mL and 0.09–0.7 μg/mL, compared to the standard HPLC-RI method. Additionally, the method was successfully applied to determine carbohydrate levels in sugar beet roots.

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超快速液相色谱-飞行时间质谱法定量甜菜根中的碳水化合物

甜菜根中蔗糖和其他碳水化合物的定量是在甜菜根加工前评估糖产量的必要条件。本研究建立了一种快速、高灵敏度和选择性的超快速液相色谱-飞行时间质谱联用（UFLC-ToFMS）方法，并验证了该方法可同时分析单糖（果糖、葡萄糖-半乳糖）、双糖（蔗糖）和三糖（棉子糖）。该方法的检出限和定量限分别为0.03 ~ 0.23 μg/mL和0.09 ~ 0.7 μg/mL，灵敏度是标准HPLC-RI方法的1000倍。此外，该方法还成功地应用于甜菜根中碳水化合物含量的测定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Chromatography B 医学-分析化学

CiteScore

5.60

自引率

3.30%

发文量

306

审稿时长

44 days

期刊介绍： The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.