Natural Products and Biotechnology最新文献

Conservation in Tissue Culture of Malacocarpus crithmifolius (Retz.) Fisch. C.A.Mey. - Relict Species from Mangyshlak Malacocarpus crithmifolius (Retz.) Fisch.C.A.Mey.- 莽吉斯拉克的稀有物种

Natural Products and Biotechnology Pub Date : 2023-06-15 DOI: 10.58465/natprobiotech.2023.02

V. Mursaliyeva, Balaussa Sarsenbek, T. Mukhanov, Akjunus Imanbayeva

{"title":"Conservation in Tissue Culture of Malacocarpus crithmifolius (Retz.) Fisch. C.A.Mey. - Relict Species from Mangyshlak","authors":"V. Mursaliyeva, Balaussa Sarsenbek, T. Mukhanov, Akjunus Imanbayeva","doi":"10.58465/natprobiotech.2023.02","DOIUrl":"https://doi.org/10.58465/natprobiotech.2023.02","url":null,"abstract":"Malacocarpus crithmifolius is an endangered rare relict with small size populations grown in a limited area. The purpose of this study was to initiate in vitro and micro replication of Malacocarpus crithmifolius. The vegetative and reproductive explants and Murashige & Skoog medium (MS) supplemented with different concentrations and combinations of cytokinin and auxins were used. The response reaction of different explants and the plant growth regulators' effects on shooting frequency, the number of shoots, callusogenesis, and rooting percent were investigated. Culturing generative explants on the MS medium containing 6-Benzylaminopurine (BAP) combined with indole-3-acetic acid (IAA) or indole-butyric acid (IBA) led to direct shoot regeneration. The bud mass appeared in the leaf axil under inflorescences after one week of cultivation on the initiated medium. The replication was reached by micro-cutting of induced additional shoots and 3-fold passaging them on the fresh mediums. The average multiplication result at MS with BAP in combination with 1-Naphthaleneacetic acid (NAA) or IAA composed 5.85 after three months of cultivation. In conclusion, the applicable explant and phytohormonal composition of nutrient medium for initiation and micropropagation of M. crithmifolius were developed.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"87 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139369702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Status of Bacterial Entomopathogens Used for Microbial Control of Arthropod Pests in Iran and Turkey 伊朗和土耳其用于节肢动物害虫微生物控制的细菌昆虫病原体现状

Natural Products and Biotechnology Pub Date : 2023-06-15 DOI: 10.58465/natprobiotech.2023.04

R. Marzban, G. B. Eroglu

引用次数: 0

Investigation of Viburnum opulus L. Apoptotic Effect on LNCaP Prostate Cancer Cell Line 研究紫云英对 LNCaP 前列腺癌细胞株的凋亡效应

Natural Products and Biotechnology Pub Date : 2023-06-15 DOI: 10.58465/natprobiotech.2023.03

Omer Gok, Fatma Secer Celik, Canan Eroglu Gunes, E. Kurar

{"title":"Investigation of Viburnum opulus L. Apoptotic Effect on LNCaP Prostate Cancer Cell Line","authors":"Omer Gok, Fatma Secer Celik, Canan Eroglu Gunes, E. Kurar","doi":"10.58465/natprobiotech.2023.03","DOIUrl":"https://doi.org/10.58465/natprobiotech.2023.03","url":null,"abstract":"The most common cancer seen in men worldwide is prostate cancer. It ranks second in the deadliest cancer types. Viburnum opulus is a type of tree that produces small red fruits. Shell, fruit, flower and juice of V. opulus are widely used in various fields and traditional medicine. Studies have shown that V. opulus also has an anti-cancer effect. For this purpose, the apoptotic effect of V. opulus was investigated in the LNCaP prostate cancer cell line. LNCaP human prostate cancer cells were treated with V. opulus extract in a 1-200 µg/mL concentration range. IC50 dose of V. opulus extract was determined by cell viability test XTT. Expressions of genes related to apoptosis after extract application were determined by quantitative RT-PCR analysis. V. opulus showed the cytotoxic effect on the LNCaP cell line, and differences in the expressions of apoptosis-related genes were observed. Significant increases in BAX, CASP7, CASP8 and P53 expressions showed that the pro-apoptosis pathway was active, while CASP3 expression was significantly decreased. The extrinsic apoptosis pathway of might be effective due to increased CASP7 and CASP8 gene expression. However, the molecular mechanism of apoptotic activity of V. opulus needs to be investigated in more detail.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"114 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139369711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the Therapeutic Effect of Sinapic Acid in Drug-Resistant and Non-Resistant Hepatocellular Carcinoma Cells 比较西那皮酸对耐药和非耐药肝细胞癌细胞的治疗效果

Natural Products and Biotechnology Pub Date : 2023-06-15 DOI: 10.58465/natprobiotech.2023.01

Fatma Secer Celik, Canan Eroglu Gunes, E. Kurar

{"title":"Comparison of the Therapeutic Effect of Sinapic Acid in Drug-Resistant and Non-Resistant Hepatocellular Carcinoma Cells","authors":"Fatma Secer Celik, Canan Eroglu Gunes, E. Kurar","doi":"10.58465/natprobiotech.2023.01","DOIUrl":"https://doi.org/10.58465/natprobiotech.2023.01","url":null,"abstract":"Sinapic acid (SA) has been shown to have anti-bacterial, anti-cancer, anti-inflammatory, cardioprotective, hepatoprotective, and neuroprotective effects in pre-clinical and in vitro investigations. Especially in cancer studies, it has been shown that SA has an anti-cancer effect. SA reduced proliferation and induced apoptosis in cancer cells. In this study, the effect of SA on drug resistance in hepatocellular carcinoma was investigated. For this purpose, cells resistant to sorafenib were obtained. According to the gene expression analysis performed in resistant cells, the expression of MDR1, hCE-1 and hCE-2 increased significantly. Then, specific doses of sinapic acid were applied to both Hep3B and resistant Hep3B cell lines. The IC50 dose for Hep3B cells was determined by XTT analysis. The cytotoxic effect of SA was different in resistant and non-resistant cells. The IC50 dose of SA was increased in resistant cells compared to Hep3B cells. The chemotherapeutic applications of herbal chemicals have been widely studied. However, drug resistance against frequently used chemotherapeutics is the biggest obstacle to treatment. This study showed that the dose of SA applied to resistant cells should be increased. This suggests that when the resistance mechanism occurs, the herbal chemicals are also effused out of the cell like drugs. It is necessary to study the intracellular interactions of SA with further studies.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139369767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Secondary Metabolites in Fungi 真菌中的次生代谢物

Natural Products and Biotechnology Pub Date : 2022-12-15 DOI: 10.58465/natprobiotech.2022.12

Birsen Atli, Beria Ozcakir, B. Isik, V. Mursaliyeva, R. Mammadov

{"title":"Secondary Metabolites in Fungi","authors":"Birsen Atli, Beria Ozcakir, B. Isik, V. Mursaliyeva, R. Mammadov","doi":"10.58465/natprobiotech.2022.12","DOIUrl":"https://doi.org/10.58465/natprobiotech.2022.12","url":null,"abstract":"Fungi are a biological family that includes multicellular and unicellular eukaryotic organisms in general. They are quite numerous and diverse. It is the second largest group of organisms on Earth after insects. Higher fungi (terrestrial fungi) currently constitute a large and efficiently untapped resource of new pharmacological crops with nutritional values are potentially powerful. Antioxidants prevent cellular damage caused by free radicals in living beings. It has been found that 36% of endophytic mushroom extracts with a high phenolic content show strong antioxidant effects. In one study, they reported that the antioxidant activities of the strains Aspergillus sp., A. niger, A. peyronelii and Chaetomium sp. ranged from 50% to 80% and that this antioxidant activity was due to their high phenolic content. In addition to the antioxidant properties of fungi, it has been shown as a result of research that they show antibacterial activity. Macrofungal β-glucans (β-1,6 glycosidic bonds and mainly β-1,3), which are known to have immunomodulatory and antitumor effects in cancer prevention, can be used in treatment strategies. At the same time, it has been shown in studies that these metabolites obtained from fungi cause fewer side effects when used in treatments, unlike chemical drugs. As a result of phytochemical research, the presence of flavonoids, anthraquinones, steroids, terpenoids, coumarins, phenols and tannins in fungal extracts in general was detected, while alkaloids and saponins were not found. It has been determined that the studies on mushrooms have gained versatile uses since they reveal secondary metabolite components with different biological activities in the structure of these group members.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117234143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Biological Evaluation of Azide Derivative as Antibacterial and Anticancer Agents 叠氮衍生物作为抗菌和抗癌剂的生物学评价

Natural Products and Biotechnology Pub Date : 2022-12-15 DOI: 10.58465/natprobiotech.2022.11

M. Yildirim, Elif Aksakal, F. B. Ozgeris, Bunyamin Ozgeris, Arzu Gormez

{"title":"Biological Evaluation of Azide Derivative as Antibacterial and Anticancer Agents","authors":"M. Yildirim, Elif Aksakal, F. B. Ozgeris, Bunyamin Ozgeris, Arzu Gormez","doi":"10.58465/natprobiotech.2022.11","DOIUrl":"https://doi.org/10.58465/natprobiotech.2022.11","url":null,"abstract":"Azides are significant compounds because of their biological effects on bacteria, viruses, fungi, and cancer. The antibacterial and anticancer properties of azide derivatives have been demonstrated in several microbial strains and cancer cell lines. The most significant microorganisms in command of hospital-acquired nosocomial infections include Acinetobacter baumannii (A. baumannii), Methicillin-resistant Staphylococcus aureus (MRSA), Candida albicans (C. albicans), and Candida dubliniensis (C. dubliniensis). These infections have become resistant to a variety of antibiotics. These resistant infections are frequently seen in cancer patients. Novel drug-active compounds are thus required that have both antibacterial and anticancer activity. This study examined previously synthesized 1-azido-5,6,7-trimethoxy-2,3-dihydro-1H-indene (4) for its antibacterial and anticancer activities. Antimicrobial activity was assessed using the disc diffusion technique, and minimum inhibitory concentration values were computed for zone formation in the examined pathogens. The study's findings indicate that while azide derivatives did not exhibit any effect against other pathogens, they suppressed bacterial growth with a zone diameter of 10 mm against A. baumannii. The MIC values of azide derivative against A. baumannii were 3.90 µg/ml. WST-8 analysis in Caco-2 cancer and healthy fibroblast cell line was used to determine the anticancer study. As a result of this analysis, the IC50 value was calculated to be 2.99 µM. There is little anticancer activity. A very low toxic effect of the azide compound on fibroblast cells was also observed. These findings suggest that this azide derivative may be tested as a potential antibacterial and anticancer agent.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116542627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological Evaluation of Aminoindane Derivatives as Antibacterial Agents 氨基酸衍生物作为抗菌药物的生物学评价

Natural Products and Biotechnology Pub Date : 2022-12-15 DOI: 10.58465/natprobiotech.2022.8

Damla Ruzgar, Yusuf Akbaba, Bunyamin Ozgeris, Arzu Gormez

{"title":"Biological Evaluation of Aminoindane Derivatives as Antibacterial Agents","authors":"Damla Ruzgar, Yusuf Akbaba, Bunyamin Ozgeris, Arzu Gormez","doi":"10.58465/natprobiotech.2022.8","DOIUrl":"https://doi.org/10.58465/natprobiotech.2022.8","url":null,"abstract":"The aminoindane ring is the most studied skeletal structure in synthetic chemistry, in the synthesis of intermediates and because it is a biologically active molecule. Bioactive properties of the aminoindane ring include antibacterial, antiviral, antiapoptotic, antipyretic, analgesic, anticonvulsant, and antiparkinsonian activities. The most important bacteria in responsibility of nosocomial infections obtained in hospitals across the world are Acinetobacter baumannii and Methicillin-resistant Staphylococcus aureus. MRSA and A. baumannii have become resistant to numerous antibiotics. The development of novel antibiotic drugs is thus urgently needed. This study as investigated at the antibacterial effects of previously synthsized compounds 8 and 9 on A. baumannii and MRSA disease microorganisms. Studies on antibacterial activity employed disc diffusion and microdilution techniques. The investigation revealed that whereas the pathogenic organisms of compounds 8 and 9 formed zones that inhibited bacterial development, no zones formed in compounds 5, 6, and 7. Compounds 8 and 9's zone diameters against A. baumannii and MRSA were estimated to be between 0.7 and 1.0 mm. The range of MIC values was 3.9025–15.625 µg/ml. These results was envisaged the conclusion that derivatives with an aminoindane ring may function as MRSA and A. baumannii antibacterial agents.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131027643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nepeta italica L. subsp. cadmea (Boiss.) A. L. Budantsev Oil Has a Pro-Apoptotic Effect on Saos-2 Cell Line 意大利荆芥亚种cadmea木香。黄芪油对Saos-2细胞的促凋亡作用

Natural Products and Biotechnology Pub Date : 2022-12-15 DOI: 10.58465/natprobiotech.2022.9

Siddika Boztas, Buket Er Urganci, Mücahit Seçme, N. Keskin

{"title":"Nepeta italica L. subsp. cadmea (Boiss.) A. L. Budantsev Oil Has a Pro-Apoptotic Effect on Saos-2 Cell Line","authors":"Siddika Boztas, Buket Er Urganci, Mücahit Seçme, N. Keskin","doi":"10.58465/natprobiotech.2022.9","DOIUrl":"https://doi.org/10.58465/natprobiotech.2022.9","url":null,"abstract":"Nepeta sp. are used diuretic, anti-spasmodic, antiasthmatic, expectorant, cough suppressant, sedative, antiseptic, antipyretic, antiallergy and as a muscle relaxant. Besides, it also has many biological activities such as antioxidant, antibacterial, antiinflammatory, anticancer, antiparasitic, antiplatelet, antiangiogenesis, antimicrobial, antiviral, antifungal, insecticide, cytotoxic, phytotoxic, genotoxic, and apoptotic activities. Osteosarcoma is the most common primary bone cancer in children and adolescents, and its diagnosis is difficult due to the known heterogeneous nature of its pathogenesis. Chemotherapy for osteosarcoma is far from satisfactory, especially because of the presence of metastases or poor response to combination chemotherapy. Various studies have been conducted to evaluate the anti-apoptotic, anticancer, and cytotoxic potentials of different essential oil extracts from different species of the Nepeta. This study was carried out to confirm the cytotoxic and apoptotic effect of essential oil obtained by hydrodistillation from the aerial parts (flower, leaf, seed and stem) of Nepeta italica subsp cadmea. To figure out apoptotic properties of N. italica subsp cadmea. MTT assay, qRT-PCR with Bcl‐2, caspase-9, Caspase-3, caspase-8, caspase-7, caspase-10, Bax gene primer and TUNEL assay accomplished. IC50 dose of N. italica subsp. cadmea oil was 10 μg/ml at 24 hours. Expression analysis showed that caspase8, caspase 10 were upregulated while Bcl-2 was downregulated. TUNEL assay results show the similar number of apoptotic cells in the control group then the Nepeta group. Therefore, it showed pro-apoptotic activity in conjunction with the expression profile.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122086864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Embryogenic Callus Differentiation in Short-Term Callus Derived from Leaf Explants of Alfalfa Cultivars 苜蓿品种叶片外植体短期愈伤组织的胚性分化

Natural Products and Biotechnology Pub Date : 2022-12-15 DOI: 10.58465/natprobiotech.2022.10

Büşra Yazıcılar, Y. Chang

{"title":"Embryogenic Callus Differentiation in Short-Term Callus Derived from Leaf Explants of Alfalfa Cultivars","authors":"Büşra Yazıcılar, Y. Chang","doi":"10.58465/natprobiotech.2022.10","DOIUrl":"https://doi.org/10.58465/natprobiotech.2022.10","url":null,"abstract":"Alfalfa is a forage crop that accounts for one of the best sources of protein and is commonly grown all over the world. In vitro callus and embryogenic induction of alfalfa have been investigated previously; however, most of the investigations were almost limited with callus formation. In this study, leaves of 5 Medicago sativa L. cultivars (Alsancak, Sazova, Plato Iside, and Bilensoy) have been used. Influences of culture medium contents and hormones applications on callus and embryogenic callus induction were determined to optimize in vitro culture mediums of alfalfa (0.0125 g kinetin 2,4 D 1 mg mL-1; 1 g kinetin 2,4 D 1 mg mL-1 ; 0.25 g kinetin 2,4 D 2 mg mL-1; 0.5 g kinetin 2,4 D 2 mg mL-1). Callus formation was detected at a rate of 74% in 5 different cultivars used in the experiment. The five alfalfa cultivars were classified into four categories in terms of embryogenic differentiation capacity. The tested alfalfa cultivars varied in their callus formation and embryogenic callus differentiation. Sazova, Alsancak, and Bilensoy were detected for better callus formation; similarly, the same cultivars responded with better embryogenic callus formation in the culture mediums including various hormone concentrations. The present study shows that our methods have beneficial impacts on the somatic embryo induced by alfalfa. However, it depends strongly on genotype, hormone concentrations and the other medium components.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127877057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A New Spectrophotometric Method for The Determination of Macitentan in Human Plasma 分光光度法测定人血浆中马西坦的新方法

Natural Products and Biotechnology Pub Date : 2022-10-29 DOI: 10.58465/natprobiotech.2022.7

O. Kocak

引用次数: 0