Investigation of Viburnum opulus L. Apoptotic Effect on LNCaP Prostate Cancer Cell Line

Natural Products and Biotechnology Pub Date : 2023-06-15 DOI:10.58465/natprobiotech.2023.03

Omer Gok, Fatma Secer Celik, Canan Eroglu Gunes, E. Kurar

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Abstract

The most common cancer seen in men worldwide is prostate cancer. It ranks second in the deadliest cancer types. Viburnum opulus is a type of tree that produces small red fruits. Shell, fruit, flower and juice of V. opulus are widely used in various fields and traditional medicine. Studies have shown that V. opulus also has an anti-cancer effect. For this purpose, the apoptotic effect of V. opulus was investigated in the LNCaP prostate cancer cell line. LNCaP human prostate cancer cells were treated with V. opulus extract in a 1-200 µg/mL concentration range. IC50 dose of V. opulus extract was determined by cell viability test XTT. Expressions of genes related to apoptosis after extract application were determined by quantitative RT-PCR analysis. V. opulus showed the cytotoxic effect on the LNCaP cell line, and differences in the expressions of apoptosis-related genes were observed. Significant increases in BAX, CASP7, CASP8 and P53 expressions showed that the pro-apoptosis pathway was active, while CASP3 expression was significantly decreased. The extrinsic apoptosis pathway of might be effective due to increased CASP7 and CASP8 gene expression. However, the molecular mechanism of apoptotic activity of V. opulus needs to be investigated in more detail.

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研究紫云英对 LNCaP 前列腺癌细胞株的凋亡效应

全球男性最常见的癌症是前列腺癌。它在最致命的癌症类型中排名第二。紫云英是一种能结出红色小果实的树。荚蒾的壳、果实、花和果汁被广泛应用于各个领域和传统医药中。研究表明，欧罂粟还具有抗癌作用。为此，我们在 LNCaP 前列腺癌细胞系中研究了欧罂粟的凋亡效应。用浓度范围为 1-200 µg/mL 的欧罂粟提取物处理 LNCaP 人类前列腺癌细胞。通过细胞活力测试 XTT 确定了欧罂粟提取物的 IC50 剂量。应用提取物后，与细胞凋亡相关的基因表达量通过定量 RT-PCR 分析确定。结果表明，V. opulus 对 LNCaP 细胞株有细胞毒性作用，并观察到细胞凋亡相关基因表达的差异。BAX、CASP7、CASP8和P53的表达量显著增加，表明促凋亡途径活跃，而CASP3的表达量则显著下降。由于 CASP7 和 CASP8 基因表达的增加，外源性凋亡途径可能有效。然而，欧鼠李凋亡活性的分子机制还需要更详细的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Natural Products and Biotechnology

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