Infection Epidemiology and Microbiology最新文献_第6页

Prevalence, Comparison of Diagnostic Methods, Antibiogram, and Genotyping of Arcobacter spp. in Diarrheal Cases Referring to Clinical Centers in Iran 伊朗临床中心腹泻病例中弧菌的患病率、诊断方法、抗生素谱和基因分型的比较

Infection Epidemiology and Microbiology Pub Date : 2022-06-01 DOI: 10.52547/iem.8.2.107

Sara Khalili Dermani, M. Akbari, M. Arjomandzadegan, A. Ahmadi

{"title":"Prevalence, Comparison of Diagnostic Methods, Antibiogram, and Genotyping of Arcobacter spp. in Diarrheal Cases Referring to Clinical Centers in Iran","authors":"Sara Khalili Dermani, M. Akbari, M. Arjomandzadegan, A. Ahmadi","doi":"10.52547/iem.8.2.107","DOIUrl":"https://doi.org/10.52547/iem.8.2.107","url":null,"abstract":"Backgrounds: Arcobacter is an emerging bacterium that may cause watery diarrhea and septicemia in humans. This study aimed to investigate the prevalence of Arcobacter spp. in diarrheal stool specimens using culture and molecular methods, their genetic diversity, and their resistance to different antibiotics in patients referring to clinical centers in Arak, Iran. Materials & Methods: In this descriptive cross-sectional study, diarrheal stool specimens were collected from 230 patients over a two-month period from July to September 2016. The samples were tested for the presence of Arcobacter species. Suspected colonies were subjected to biochemical tests and identified by phenotypic methods. In addition, antimicrobial susceptibility testing was performed using the disk diffusion method. were also directly detected by multiplex-PCR. Findings: Out of 230 samples, 20 samples (8.69%) were positive in culture method, and 44 samples (19.13%) were positive in PCR method, all culture-positive samples were also positive in PCR method. Rep-PCR indicated 14 different rep types among Arcobacter spp. isolated from patients with gastroenteritis. All Arcobacter isolates were resistant to cefazolin, ceftazidime, and nalidixic acid. The isolates showed high susceptibility to tetracycline, gentamicin, ampicillin, amikacin, meropenem, erythromycin, and ciprofloxacin. Conclusions: To the best of our knowledge, this is the first study conducted in Iran to isolate Arcobacter spp. from patients with gastroenteritis. The results indicate that Arcobacter spp. are one of the main causes of acute diarrhea in humans. The research outcomes show that Arcobacter spp. could be considered as the etiology of gastrointestinal infections in humans.","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89751633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virulence Genes and Antibiotic Susceptibility of Enterococcus spp. in Bandar Abbas City, Iran 伊朗阿巴斯市肠球菌的毒力基因和抗生素敏感性

Infection Epidemiology and Microbiology Pub Date : 2022-06-01 DOI: 10.52547/iem.8.2.129

T. Dehghani, A. Karmostaji, H. Alizade

引用次数: 1

Evaluation of Anticancer Potential of Silver Chloride NanoparticlesBiosynthesized by Penicillium chrysogenum 由青霉菌合成的纳米氯化银抗癌潜力的评价

Infection Epidemiology and Microbiology Pub Date : 2022-06-01 DOI: 10.52547/iem.8.2.159

Setareh Zamani, M. Fazilati, Manijeh Hadian, H. Nazem, N. Noohi

{"title":"Evaluation of Anticancer Potential of Silver Chloride Nanoparticles\u0000Biosynthesized by Penicillium chrysogenum","authors":"Setareh Zamani, M. Fazilati, Manijeh Hadian, H. Nazem, N. Noohi","doi":"10.52547/iem.8.2.159","DOIUrl":"https://doi.org/10.52547/iem.8.2.159","url":null,"abstract":"Green synthesis of nanoparticles (NPs) is a simple, fast, and eco-friendly method which could be performed by various microorganisms or plant extracts. Silver NPs are well-known as antimicrobial and anti-fungal materials. They play an essential role in the control of tumors via their cytotoxic effects. Therefore, they have attracted significant attention for developing an effective treatment solution for cancer cells. This study aimed to investigate the potential of Penicillium chrysogenum for the synthesis of silver NPs and to evaluate their toxicity on liver cancer cell line (HepG2). , characterization of the synthesized NPs was performed by UV–Vis spectroscopy, X-ray diffraction (XRD), and transmission electron microscopy (TEM). Fourier transform infrared spectroscopy (FTIR) was carried out to detect biomolecules that may be responsible for the synthesis and stabilization of NPs. The cytotoxic activity of the synthesized AgclNPs on HepG2 cell line was evaluated using MTT assay. Findings: UV–Vis spectroscopy and XRD analysis confirmed the synthesis of AgclNPs using P. chrysogenum . TEM analysis revealed the spherical shape of AgclNPs with an average crystalline size of 15 to 45 nm. FTIR spectroscopy indicated the possible functional groups that could be responsible for the reduction of metal ions and the capping process. These nanoparticles showed a dose-dependent anticancer activity against HepG2 cells. Conclusion: The results suggest that biosynthesized silver chloride nanoparticles could offer potential applications in cancer therapy.","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84800250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Design and Synthesis of Novel Thymoquinone-Zein Nanoparticles; Evaluation of the Inhibitory Effect on Candida albicans and Biofilm Formation in Vitro 新型百里醌-玉米苷元纳米颗粒的设计与合成体外对白色念珠菌及生物膜形成的抑制作用评价

Infection Epidemiology and Microbiology Pub Date : 2022-06-01 DOI: 10.52547/iem.8.2.169

S. Rahsepar, Shahla Roudbar mohammadi‎, H. Delavari, M. Roudbari, Zuhair Mohammad Hassan

{"title":"Design and Synthesis of Novel Thymoquinone-Zein Nanoparticles; Evaluation of the Inhibitory Effect on Candida albicans and Biofilm Formation in Vitro","authors":"S. Rahsepar, Shahla Roudbar mohammadi‎, H. Delavari, M. Roudbari, Zuhair Mohammad Hassan","doi":"10.52547/iem.8.2.169","DOIUrl":"https://doi.org/10.52547/iem.8.2.169","url":null,"abstract":"Design and Syn- thesis of Novel Thymoquinone-Ze-in Nanoparticles; Evaluation of the Inhibitory Effect on Candida albicans and Biofilm in Vitro. Infection Epidemiology and Microbiology. 2022;8(2): ingredient in Nigella sativa, which has considerable antifungal properties. The aim of this study was to investigate the inhibitory effects of thymoquinone-zein nanoparticles (TQ-ZNPs) on In the current study, TQ was encapsulated in zein (as a biodegradable carrier) and polyethylene glycol (PEG). The antifungal activity of TQ-ZNPs against (ATCC 10231; standard strain) and their inhibitory effects on biofilm formation were examined using standardized broth microdilution and MTT assays, respectively. The total oxidant status (TOS) of C. albicans was assessed using colorimetric method, and the toxic effect of nanoparticles on peripheral blood mononuclear cells (PBMCs) was evaluated by MTT assay. The minimum inhibitory concentration (MIC) of TQ-ZNPs was significantly reduced compared to that of free TQ. MIC values of TQ-ZNPs and free TQ were determined to be 7.4 and 50 µg/mL , respectively. Biofilm formation was inhibited, and oxidant production by fungal cells was increased. The findings of this study showed that TQ-ZNPs had no toxic effect on PBMCs. Conclusion: This study results revealed that the synthesized nanoparticles had a good antifungal activity without any toxicity. The results demonstrated the superior efficiency of TQ-ZNPs over free TQ. Hence, this structure could be used to load hydrophobic drugs. However, more studies are needed to evaluate the beneficial properties of TQ-ZNPs. and fluconazole (0.5 MIC, 1 MIC, and 2 MIC)","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86881117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Allium cepa Inhibits Aspergillus fumigatus Growth and Virulence and Suppresses the Expression of glip Gene Involved in Fungal Pathogenesis 大蒜抑制烟曲霉生长、毒力及抑制glip基因表达的研究

Infection Epidemiology and Microbiology Pub Date : 2022-06-01 DOI: 10.52547/iem.8.2.149

Bahareh Musivand, M. Shams-Ghahfarokhi, Mehdi Razzaghi Abyaneh

引用次数: 0

Isolation and in Vitro Characterization of Specific Bacteriophages against Methicillin-Resistant Staphylococcus aureus 耐甲氧西林金黄色葡萄球菌特异性噬菌体的分离及体外鉴定

Infection Epidemiology and Microbiology Pub Date : 2022-06-01 DOI: 10.52547/iem.8.2.121

M. Esmaeilzadeh, S. Sabzi, Aida Hajihossein Tabrizi

{"title":"Isolation and in Vitro Characterization of Specific Bacteriophages against Methicillin-Resistant Staphylococcus aureus","authors":"M. Esmaeilzadeh, S. Sabzi, Aida Hajihossein Tabrizi","doi":"10.52547/iem.8.2.121","DOIUrl":"https://doi.org/10.52547/iem.8.2.121","url":null,"abstract":"Specific Bacteriophages against Methicil-lin-Resistant Bacteriophage therapy could be an alternative strategy for the treatment of antibiotic-resistant bacteria. This study aimed to evaluate the antibacterial activities of isolated bacteriophages against methicillin-resistant Staphylococcus aureus (MRSA) isolates. Materials & Methods: A total of 16 clinical isolates of MRSA were collected from medical diagnostic laboratories in Tehran, Iran. A specific bacteriophage was isolated from hospital sewage using double-layer agar. Phage morphology was evaluated by transmission electron microscopy (TEM). Different bacteria were selected to determine the bacteriophage host range using spot test. Phage susceptibility to temperature and pH was evaluated by double-layer agar method. In vitro assay was carried out on human epithelial type 2 (HEp-2) cells to investigate the effect of bacteriophage on the adhesion of MRSA to human epithelial cells. Findings : TEM suggested the Myoviridae family for the isolated phage. The effective titer of bacteriophages was 1.8×10 The bacteriophage 4 and pH=8. The isolated bacteriophage was specific for all clinical isolates of MRSA and had no lytic activity against other pathogenic bacteria. In evaluating the binding and invasion of MRSA to the HEp-2 cell line, as expected, the lytic activity of specific bacteriophages was observed following inoculation. Conclusion: The specificity and lytic activity of this phage on MRSA and MRSA-infected HEp-2 cell line emphasized that the isolated bacteriophage may serve as an effective prophylactic and alternative therapeutic agent in hospital settings.","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73453408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Typing of Streptococcus agalactiae- cMLSB Phenotype Isolates by Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) in Isfahan, Iran 伊朗伊斯法罕地区无乳链球菌- cMLSB表型分离株的重复基因间共识pcr (ERIC-PCR)分子分型

Infection Epidemiology and Microbiology Pub Date : 2022-06-01 DOI: 10.52547/iem.8.2.139

Saba Jalalifar, Tahereh Motallebirad, Shirin Dashtbin, Rasoul Mirzaei, M. Khorshidi, Bahram Nasr Esfahani

{"title":"Molecular Typing of Streptococcus agalactiae- cMLSB Phenotype Isolates by Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) in Isfahan, Iran","authors":"Saba Jalalifar, Tahereh Motallebirad, Shirin Dashtbin, Rasoul Mirzaei, M. Khorshidi, Bahram Nasr Esfahani","doi":"10.52547/iem.8.2.139","DOIUrl":"https://doi.org/10.52547/iem.8.2.139","url":null,"abstract":"B antibiotics (cMLSB phenotype) has become a global concern. On the other hand, little is known about the genetic relatedness and diversity of GBS isolates isolated from various patients in Iran. Hence, this study aimed to determine the genetic relatedness and molecular typing of cMLSB-GBS isolates using enterobacterial repetitive intergenic consensus-PCR (ERIC- PCR) technique. Materials & Methods: A total of 100 GBS isolates were collected from patients with urinary tract infections (UTIs). Among them, 52 erythromycin-resistant GBS isolates were selected, and double-disc diffusion (D-zone) technique was applied to determine the MLSB phenotype among the isolates based on CLSI criteria. Then the genetic relatedness of MLSB-GBS isolates was assessed using ERIC-PCR fingerprinting method. Findings: Among 52 erythromycin-resistant GBS isolates, 38 isolates were identified with cMLSB phenotype, nine isolates with M phenotype, and five isolates with iMLSB phenotype. The analysis of ERIC-PCR patterns revealed eight different ERIC types that were divided into seven clusters (A-G) and one single type. Also, four isolates were non-typeable. ERIC type A/ serotype Ib was the most prevalent clone among the isolates. Conclusion: The current study findings showed a high level of diversity and multiclonal spread of the cMLSB phenotype in Isfahan. ERIC type A/ serotype Ib is the predominant clone circulating among erythromycin-resistant GBS strains.","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83353357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Association between Hematology Indices as Early Markers and Urinary Tract Infection, Septicemia, Pneumonia, and Diabetic Foot Infection 血液学指标作为早期标志物与尿路感染、败血症、肺炎和糖尿病足感染的关系

Infection Epidemiology and Microbiology Pub Date : 2022-02-01 DOI: 10.52547/iem.8.1.7

A. Majidpour, Nastaran Arianpor, Sara Fathizadeh, Samira Rasouli koohi, Somayeh Soleymanzadeh moghaddam

{"title":"Association between Hematology Indices as Early Markers and Urinary Tract Infection, Septicemia, Pneumonia, and Diabetic Foot Infection","authors":"A. Majidpour, Nastaran Arianpor, Sara Fathizadeh, Samira Rasouli koohi, Somayeh Soleymanzadeh moghaddam","doi":"10.52547/iem.8.1.7","DOIUrl":"https://doi.org/10.52547/iem.8.1.7","url":null,"abstract":"they were hospitalized in Rasoul Akram hospital in Iran from August 2017 to February 2018. Patients’ demographic and laboratory data, such as RDW (red cell distribution width), PDW (platelet distribution width), RBC (red blood cell), CRP (C-reactive protein), ESR (erythrocyte sedimentation rate), and, WBC (white blood cells), were evaluated. Findings: This study results showed that mortality rate in sepsis cases was higher than in other cases (42.1%). Changes in blood parameters such as RDW, PDW, and EDR levels as well as monocyte, basophil, and eosinophil counts were significant among patients with different infectious diseases, while there was no significant difference in terms of changes in some blood parameters, such as WBC, neutrophil, and lymphocyte counts and CRP level between patients with different infectious diseases. For statistical analysis, one-way ANOVA and LSD post hoc tests were used. Conclusion: According to this study results, it was found that the range of blood parameters varies in different types of infectious diseases. Therefore, the could employ routine blood parameters along with other diagnostic factors to more accurately diagnose the type of infection prescribe appropriate","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72790813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Engineered Mesenchymal Stem Cell by Lentiviral Vector Expressing CD44 as a Candidate to Target Colon Cancer Tissue in Mice Model 慢病毒载体表达CD44的工程化间充质干细胞作为结肠癌小鼠模型的候选靶向组织

Infection Epidemiology and Microbiology Pub Date : 2022-02-01 DOI: 10.52547/iem.8.1.69

A. Nabizadeh, M. Ravanshad

引用次数: 0

Effects of Eugenol-Loaded Chitosan Biopolymer Nanoparticles on CYP51A and CYP51B Expression in Aspergillus fumigatus 载丁香酚壳聚糖纳米生物聚合物对烟曲霉CYP51A和CYP51B表达的影响

Infection Epidemiology and Microbiology Pub Date : 2022-02-01 DOI: 10.52547/iem.8.1.17

Abozar Nasiri-Jahrodi, M. Shams-Ghahfarokhi, Mehdi Razzaghi Abyaneh

{"title":"Effects of Eugenol-Loaded Chitosan Biopolymer Nanoparticles on CYP51A and CYP51B Expression in Aspergillus fumigatus","authors":"Abozar Nasiri-Jahrodi, M. Shams-Ghahfarokhi, Mehdi Razzaghi Abyaneh","doi":"10.52547/iem.8.1.17","DOIUrl":"https://doi.org/10.52547/iem.8.1.17","url":null,"abstract":"Backgrounds: Aspergillus fumigatus is a pathogen responsible for invasive aspergillosis and the main leading cause of death in immunosuppressed individuals. The present study aimed to evaluate the impact of eugenol-loaded chitosan nanoparticles on the expression of CYP51a and CYP51b , two well-known genes responsible for triazole drug resistance in A. fumigatus . The minimum inhibitory concentration (MIC) of eugenol-loaded chitosan nanoparticles, chitosan, eugenol, and itraconazole was determined based on the Clinical and Laboratory Standards Institute M38-E3 method at concentrations of 4.6-2400, 11.7-12000, 2-2048, and 1-256 μ g/mL, respectively. The expression of CYP51A and CYP51B was evaluated in A. fumigatus exposed to 0.5, 1, and 2× of MIC concentration of NPs and itraconazole using the real-time polymerase chain reaction. Findings: The obtained results showed that eugenol-loaded chitosan nanoparticles sucessfully reduced eugenol, and itraconazole was measured to be 6000, 256, and 4 μg/mL, respectively. The results of real-time PCR also revealed that eugenol-loaded chitosan nanoparticles increased the expression of both CYP51A and CYP51B in a dose-dependent manner. The expression of fungal CYP51A and CYP51B at mRNA level was significantly increased 1.26, 1.93, and 3.1-fold as well as 1.2, 2.1, and 2.4-fold at concentrations of 150, 300, and 600 μg/mL , respectively ( p <.05). However, it seems that the prepared nanoparticles had a lower impact on the expression of these genes compared to itraconazole. findings the treatment of with eugenol-chitosan nanoparticles increase the of the CYP51 suggesting the antifungal property of these nanoparticles.","PeriodicalId":34545,"journal":{"name":"Infection Epidemiology and Microbiology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76900439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0