大蒜抑制烟曲霉生长、毒力及抑制glip基因表达的研究

Q4 Medicine

Infection Epidemiology and Microbiology Pub Date : 2022-06-01 DOI:10.52547/iem.8.2.149

Bahareh Musivand, M. Shams-Ghahfarokhi, Mehdi Razzaghi Abyaneh

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引用次数: 0

摘要

观察0.5× MIC浓度的EAC (1000 μg/mL)和氟康唑(64 μg/mL)对真菌胶质毒素产生、细胞膜麦角甾醇含量、超微结构和gliP基因表达的影响。结果:经eac和fcz处理的真菌细胞麦角甾醇含量分别显著降低至0.53和0.45µg/mg (p < 0.001)。EAC和fcz处理组的蛋白酶活性均受到显著抑制。FCZ和EAC对胶质毒素产生的抑制作用分别为51.55%和68.75%。EAC-和fcz -处理组gliP表达量分别显著降低0.40倍和0.53倍(p < 0.05)。结论:本研究结果提示该菌的生长和毒力生物活性有待进一步鉴定

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Allium cepa Inhibits Aspergillus fumigatus Growth and Virulence and Suppresses the Expression of glip Gene Involved in Fungal Pathogenesis

gliotoxin production, cell membrane ergosterol content, ultrastructure, and gliP gene expression were evaluated in the fungus exposed to 0.5× MIC concentrations of EAC (1000 μg/mL) and fluconazole (FCZ: 64 μg/mL). Findings: Ergosterol content was significantly reduced to 0.53 and 0.45 µg/mg in EAC-and FCZ-treated fungal cells, respectively ( p < .001). The protease activity was significantly inhibited in both EAC- and FCZ-treated groups. The gliotoxin production was inhibited by 51.55 and 68.75% in the treated groups with FCZ and EAC, respectively. The expression of gliP in both EAC- and FCZ-treated was significantly reduced by 0.40 and 0.53-fold, respectively ( p < .05). Conclusion: This study findings revealed that growth and virulence bioactive Further are recommended to identify

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来源期刊

Infection Epidemiology and Microbiology Medicine-Epidemiology

CiteScore

0.50

自引率

0.00%

发文量

审稿时长

6 weeks