Bulletin of Pharmaceutical Sciences. Assiut最新文献

{"title":"The therapeutic potential of cold atmospheric plasma against pathogenic bacteria inhabiting diabetic wounds","authors":"Gamal Badr, Fayez El-Hossary, M. Salah, M. Khalaf, E. Sayed, Ahmed Elminshawy","doi":"10.21608/bfsa.2023.216384.1770","DOIUrl":"https://doi.org/10.21608/bfsa.2023.216384.1770","url":null,"abstract":"Impaired healing process of diabetic wounds is a common problem among diabetic patients. These diabetic wounds are slow to heal and can lead to serious complications if left untreated. One of the reasons diabetic wounds are slow to heal is that pathogenic bacteria often colonize them. Cold atmospheric plasma (CAP) is a promising new technology that has been shown to effectively kill and inhibit pathogenic bacteria in diabetic wounds. In the current study, we examined by what means CAP works and its potential impact as a diabetic wound treatment. Fifty diabetic patients aged from 20 to 60 years old with diabetic wounds were included in our study using the swab method. We used general and specific culture media to isolate the pathogenic bacteria from diabetic wounds and identified the bacteria via different techniques using a light microscope (oil lens) and VITEK. Moreover, we confirmed the identification of isolated bacteria by 16S rRNA. Our results demonstrated the direct anti-bacterial effect of CAP against the isolated bacteria. The most prominent bacterial type in diabetic wounds was Staphylococcus sp. CAP demonstrated the highest effect against Bacillus sp. and Paenibacillus sp., which were responsible for relevant changes in the skin and were associated with the skin immune response and microbiota. Our results revealed the therapeutic use of CAP as a powerful strategy to overcome the invading pathogenic bacteria that colonize diabetic wounds and hence improve the healing process of diabetic wounds.","PeriodicalId":345040,"journal":{"name":"Bulletin of Pharmaceutical Sciences. Assiut","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125057060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disturbances in some plasma amino acids metabolomic profiles and their derivatives in Egyptian obese women","authors":"Abdel Rahman Abdel qawy, T. Saleem, R. Salama, E. Radwan, Ghada Mohamed","doi":"10.21608/bfsa.2023.209233.1742","DOIUrl":"https://doi.org/10.21608/bfsa.2023.209233.1742","url":null,"abstract":"Background: Obesity is a risk factor for several lifestyle diseases such as type II diabetes, hypertension, cancer and others. Studies have shown that plasma amino acids profile may be useful for screening lifestyle diseases. This case-control study aimed at investigating disturbances in acidic, basic, neutral, sulfur-containing amino acids besides imino amino acids profiles in Egyptian obese women to reach a healthy body and to stop any future complications of overweight. Methods: A total of 85 women were enrolled in this study that were classified into 5 groups (17 persons each):1-healthy, 2-overweights, 3-moderate obese, 4-severe obese, 5-morbid obese. Plasma metabolomic profile of the previous amino acids was evaluated using amino acid analyzer. Results: A statistically significant ascending increase in the mean plasma levels of citrulline, glutamate, proline and cysteine together with significant stepwise decrease in ornithine, serine, threonine, aspartate, histidine, arginine, methionine & taurine in obese subgroups as compared to controls. Conclusion: Our results of multivariate regression analysis demonstrated that the significantly elevated circulating lysine or proline levels were the best independent predictors for recognizing obese patients at risk of associated metabolic disorders and that the plasma AA metabolomic profile can be used as important biomarkers for early prediction of overweight women. Amino acids-directed regimens intervention could have significant impact on obesity management.","PeriodicalId":345040,"journal":{"name":"Bulletin of Pharmaceutical Sciences. Assiut","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129110638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CELECOXIB NIOSOMAL IN-SITU GEL AS A VALUABLE DRUG DELIVERY SYSTEM FOR OCULAR INFLAMMATION","authors":"Mohamed Ahmed, I. Khalil, Khaled M. A. Hassanein, G. El-Gendy, S. Ismail","doi":"10.21608/bfsa.2023.217860.1780","DOIUrl":"https://doi.org/10.21608/bfsa.2023.217860.1780","url":null,"abstract":"Niosomes are promising nanocarriers for ocular drug administration since they have the potential to enhance the bioavailability and efficacy of different drugs. Meanwhile, topical gels are beneficial for the treatment of ocular inflammation as they improve corneal permeability and increase the contact time with the eye surface. The main purpose of this study was to prepare and evaluate novel niosomal gels for intraocular delivery of celecoxib. Different niosomes were prepared using different surfactants (span 60 and span 40) and cholesterol (30-50 mol %). The optimized formulation made with span 40 and cholesterol (7:3 molar ratio) has a relatively high encapsulation efficiency (~57%) with reasonable particle size for ocular delivery (~348 nm) and showed the highest release of ~65% after 24 hrs compared to other formulations. The optimized niosomal formulation was then used to prepare various formulations of celecoxib in-situ gel and topical hydrogels. The niosomal gels were well tolerated by the eye and showed similar celecoxib corneal permeation. However, the in-situ gel showed a higher anti-inflammatory effect compared to the topical hydrogel and a commercially available diclofenac eye drop. The results shown in this study indicate that celecoxib niosomal in-situ gel is a valuable drug delivery system for ocular inflammation.","PeriodicalId":345040,"journal":{"name":"Bulletin of Pharmaceutical Sciences. Assiut","volume":"66 48","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114005489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances and developments in drug repurposing; state of art","authors":"Sarmad D Noori, mais Alhamdani, Amira B. Kassem","doi":"10.21608/bfsa.2023.213566.1754","DOIUrl":"https://doi.org/10.21608/bfsa.2023.213566.1754","url":null,"abstract":"Although the rapid progress in science and technology, developing new drugs from scratch has been expensive in recent decades. As a result, drug-repurposing, which implies seeking new utilizes for presently approved drugs instead of discovering new drug compounds, has emerged as a substitution tool to speed up the drug development procedure. Nowadays, drug repurposing accounts for 30% of newly approved drugs. With the explosive growth of pharmacological compounds' molecular, genomic, and phenotypic data, a novel area of drug repurposing called computational drug repurposing has emerged. This review provides an overview of recent progress in computational drug repurposing, including repositioning approaches, commonly used computational methods, validation techniques for repurposing studies, and the remaining encounters in computational repurposing. In this Review, we outline methods for drug repurposing (also known as drug repositioning), talk about difficulties encountered by the repurposing community, and suggest creative solutions to these issues. addressed to aid in maximising the benefits of medicinal repurposing.","PeriodicalId":345040,"journal":{"name":"Bulletin of Pharmaceutical Sciences. Assiut","volume":"200 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116651719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol lipid based nanoparticles show a promising outcome on induced non alcoholic fatty liver disease","authors":"D. Abdelmalek, Basma Abd EL Hamid, Noura H. Abdellah, S. Ismail","doi":"10.21608/bfsa.2023.214630.1759","DOIUrl":"https://doi.org/10.21608/bfsa.2023.214630.1759","url":null,"abstract":"","PeriodicalId":345040,"journal":{"name":"Bulletin of Pharmaceutical Sciences. Assiut","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130727141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation and preparation of atenolol-loaded floating microcapsules","authors":"Hayder A. Hammoodi, Marwa S El-Dahan, T. Borg","doi":"10.21608/bfsa.2023.214057.1756","DOIUrl":"https://doi.org/10.21608/bfsa.2023.214057.1756","url":null,"abstract":"The aim of this investigation is to prepare Atenolol (AT) floating microcapsules with sustained release profiles utilizing ethyl cellulose (EC) as a matrix former. EC is frequently used as a hydrophobic polymeric material for extended drug release applications. Emulsion-solvent evaporation technique was utilized to fabricate the floating microcapsules. The ratios of EC and AT utilized were 1:1, 1:2, 1:3, 1:4","PeriodicalId":345040,"journal":{"name":"Bulletin of Pharmaceutical Sciences. Assiut","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115316384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"POLYMERIC NANOPARTICLES AS ANTICANCER DRUG DELIVERY SYSTEMS OF CERTAIN PHYTOCHEMICALS: A COMPREHENSIVE OVERVIEW","authors":"Mona Y. A. Mostafa, M. Amin, Shady M Abd El-Halim","doi":"10.21608/bfsa.2023.217297.1778","DOIUrl":"https://doi.org/10.21608/bfsa.2023.217297.1778","url":null,"abstract":"Cancer remains a major global health concern with high fatality rates despite the existing therapeutic approaches. Although chemotherapy, radiotherapy, and surgical resection have significantly lowered cancer mortality, the survival rate remains low as a result of their adverse effects, including hepatotoxicity, cardiac cytotoxicity, nephrotoxicity, gastrointestinal toxicity, myelosuppression, mucositis, neurotoxicity","PeriodicalId":345040,"journal":{"name":"Bulletin of Pharmaceutical Sciences. Assiut","volume":"126 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122490880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of Essential oils of selected plants on clinical isolates of Candida Species growth, transition and biofilm formation","authors":"A. Amen, Ehsan Hasan Ahmed, S. Elgendy, S. Bayoumi, Muhamad R. Abdel Hameed, E. Abd-Alrahman","doi":"10.21608/bfsa.2023.207806.1735","DOIUrl":"https://doi.org/10.21608/bfsa.2023.207806.1735","url":null,"abstract":"Candida species are one of common opportunistic pathogens in humans. Detection of antifungal resistance between candida species become increasing, a great interest in creating new antifungals utilizing natural sources like plant essential oils (EOs) . This study aimed to evaluate the effect of EOs of selected plants on Candida species growth, transition, and biofilm formation as well on the expression levels of certain virulence genes. Patients and methods: Fifty candida isolates were collected from napkin dermatitis and oral candidiasis patients, different Candida species were identified phenotypically. Essential oils for some medicinal plants (basil, clove, garlic, and peppermint) were extracted by hydro-distillation. Their antifungal activity was assessed by minimal inhibitory concentration methods. In addition the effect of EOs on different Candida ultrastructure was detected by scanning electron microscope, the antibiofilm characteristics were examined by microtiter plate method. The ability of tested EOs to interfere with transition of candida from blastopore to hyphae form was evaluated. The effect of basil and clove oils on various virulent genes was determined by analyzing the expression levels of HWP, ALS3, SAP3 genes by RT-PCR. Results : C. albicans was the most prevalent species. For all tested Candida isolates, the EOs displayed a high antifungal activity. The EOs decreased the Candida transition, disrupted the Candida ultrastructure and suppressed their ability of biofilm formation, clove oil exposure significantly downregulated the expression of HWP1, ALS3 and SAP3 genes, in addition basil oil downregulated the expression of ALS3 and SAP3. Conclusion : This study concluded that clove oil is the best essential oil among other EOs that inhibited growth, transition and biofilm formation by lowest MIC and downregulated HWP1, AlS3 and SAP3 genes, which suggest their potential application for its use as antifungal therapy.","PeriodicalId":345040,"journal":{"name":"Bulletin of Pharmaceutical Sciences. Assiut","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131422998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metformin and Carvedilol ameliorate isoprenaline-induced myocardial infarction in non-diabetic rats through AMPK activation and suppression of apoptosis","authors":"Eman Abd Almalik Abd Ali, A. Abouelella","doi":"10.21608/bfsa.2023.208021.1736","DOIUrl":"https://doi.org/10.21608/bfsa.2023.208021.1736","url":null,"abstract":"The objective of this study was to clarify the ameliorative effect of metformin (MET), carvedilol (CAR)","PeriodicalId":345040,"journal":{"name":"Bulletin of Pharmaceutical Sciences. Assiut","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127306901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDENTICAL ANTIMICROBIAL EFFECT OF CEFTRIAXONE AND CEFOTAXIME AMONG DIFFERENT BACTERIAL ISOLATES","authors":"Khaled Abu El Aish, M. Taleb, A. H. el Bilbeisi","doi":"10.21608/bfsa.2023.211298.1748","DOIUrl":"https://doi.org/10.21608/bfsa.2023.211298.1748","url":null,"abstract":"Background: This study aimed to screen the antimicrobial-resistant profile of these 3rd generation cephalosporins and to identify their similarity and interchangeability. Method: This cross-sectional study, was conducted in all the government hospitals across the Gaza Strip, Palestine. The study started in November 2017 and continued till December 2022. All clinical samples such as wound swabs (pus), urine, sputum, blood, cerebrospinal fluid (CSF), stool, and others in which ceftriaxone and cefotaxime were examined at the same time were collected from hospitalized patients and outpatient clinic attendants. After the identification of the bacterial isolates, a standard disc diffusion technique for drug susceptibility tests was performed. This study was reviewed and approved by The Palestinian Helsinki Committee. Results: In the current study, 24,120 isolates in which ceftriaxone and cefotaxime were examined at the same time were studied. The predominant organisms isolated were, Escherichia coli 9,720 (40.3%), Klebsiella spp. 5,497 (22.8%), Pseudomonas spp. 2,630 (10.9%), and Staphylococcus aureus 1941 (8.0%). Bacterial isolates showed 57.1% and 57.8% resistance against ceftriaxone and cefotaxime, respectively. In this study, 22,404 (92.9%) bacterial isolates were with identical susceptibility test results to ceftriaxone and cefotaxime. The highest match of susceptibility was seen in Acinetobacter spp. 97.3% (770/791), Klebsiella spp. 95.3% (5,241/5,491), and Escherichia coli 94.9% (9,220/9,720). In Staphylococcus aureus it was 90.6% (1,758/1,941). Conclusion: Ceftriaxone and cefotaxime can be interchangeable in most Enterobacteriaceae bacterial species. Ongoing surveillance of different bacterial antimicrobial resistance and multidrug resistance is strongly recommended together with the consideration of implementing antibiotics stewardship programs in all hospitals.","PeriodicalId":345040,"journal":{"name":"Bulletin of Pharmaceutical Sciences. Assiut","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128477893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}