BioTech最新文献 - Book学术

Direct Shoot Regeneration from the Finger Millet's In Vitro-Derived Shoot Apex and Genetic Fidelity Study with ISSR Markers. 五味子离体茎尖直接再生及ISSR标记遗传保真度研究。

IF 2.7

BioTech Pub Date : 2025-04-18 DOI: 10.3390/biotech14020029

Theivanayagam Maharajan, Veeramuthu Duraipandiyan, Thumadath Palayullaparambil Ajeesh Krishna

{"title":"Direct Shoot Regeneration from the Finger Millet's In Vitro-Derived Shoot Apex and Genetic Fidelity Study with ISSR Markers.","authors":"Theivanayagam Maharajan, Veeramuthu Duraipandiyan, Thumadath Palayullaparambil Ajeesh Krishna","doi":"10.3390/biotech14020029","DOIUrl":"https://doi.org/10.3390/biotech14020029","url":null,"abstract":"Globally, people are cultivating finger millet, an important cereal, to improve food availability and health benefits for humans. However, the biotechnological research on this millet is limited and insufficient in this field. The primary focus of this study is to optimize an efficient regenerated protocol for initiating further plant transformation studies, using the shoot apex as an explant and various growth regulators. For example, three cytokinins (BAP, TDZ, and Kin) at different concentrations were used to induce multiple shoots of finger millet. Among these, TDZ (4.5 µM) provided the maximum number (17.3) of shoots as compared to BAP and Kin. IBA (2.46 µM), along with MS medium, was used for the induction of roots, where 5.6 roots were produced in an individual shoot and the length of the root was longer with a size of 8.2 cm after two weeks of incubation. The clonal fidelity of the in vitro regenerated plantlets of finger millet was confirmed by ISSR primers. Overall, the present work developed a robust and reliable procedure for the establishment of efficient and reproducible regeneration through the shoot apex that will be useful for the genetic improvement of this crop. The genetic enhancement of these millets as well as the successful creation of transgenic plant varieties modified for resistance to biotic and abiotic challenges in the near future would be aided by this study.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Matrix Metalloproteinases in Glioma: Drivers of Invasion and Therapeutic Targets. 基质金属蛋白酶在胶质瘤中的作用：侵袭的驱动因素和治疗靶点。

IF 2.7

BioTech Pub Date : 2025-04-15 DOI: 10.3390/biotech14020028

Ella E Aitchison, Alexandra M Dimesa, Alireza Shoari

{"title":"Matrix Metalloproteinases in Glioma: Drivers of Invasion and Therapeutic Targets.","authors":"Ella E Aitchison, Alexandra M Dimesa, Alireza Shoari","doi":"10.3390/biotech14020028","DOIUrl":"https://doi.org/10.3390/biotech14020028","url":null,"abstract":"Matrix metalloproteinases (MMPs) are a family of zinc-dependent proteolytic enzymes that are crucial for the remodeling of the extracellular matrix, a process that is often co-opted by cancers, including brain tumors, to facilitate growth, invasion, and metastasis. In gliomas, MMPs contribute to a complex interplay involving tumor proliferation, angiogenesis, and immune modulation, thereby influencing tumor progression and patient prognosis. This review provides a comprehensive analysis of the roles of various MMPs in different types of gliomas, from highly malignant gliomas to metastatic lesions. Emphasis is placed on how the dysregulation of MMPs impacts tumor behavior, the association between specific MMPs and the tumor grade, and their potential as biomarkers for diagnosis and prognosis. Additionally, the current therapeutic approaches targeting MMP activity are discussed, exploring both their challenges and future potential. By synthesizing recent findings, this paper aims to clarify the broad significance of MMPs in gliomas and propose avenues for translational research that could enhance treatment strategies and clinical outcomes.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144045317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AsNAC Genes: Response to High Mercury Concentrations in Allium sativum Seed Clove. 大蒜种子丁香AsNAC基因对高汞浓度的响应。

IF 2.7

BioTech Pub Date : 2025-04-08 DOI: 10.3390/biotech14020027

Brenda Mendoza-Almanza, María de la Luz Guerrero-González, Marcos Loredo-Tovias, María Elena García-Arreola, Catarina Loredo-Osti, Erika Padilla-Ortega, Pablo Delgado-Sánchez

{"title":"AsNAC Genes: Response to High Mercury Concentrations in Allium sativum Seed Clove.","authors":"Brenda Mendoza-Almanza, María de la Luz Guerrero-González, Marcos Loredo-Tovias, María Elena García-Arreola, Catarina Loredo-Osti, Erika Padilla-Ortega, Pablo Delgado-Sánchez","doi":"10.3390/biotech14020027","DOIUrl":"https://doi.org/10.3390/biotech14020027","url":null,"abstract":"Heavy metal contamination in soils is a growing concern due to anthropogenic activities, and Allium sativum (garlic) has shown tolerance to mercury pollution. We analyzed the physiological and molecular responses of garlic cloves exposed to HgCl2 at 0, 5000, 23,000, and 46,000 mg/kg for 2, 3, and 4 h. The germination percentage was lower than 46,000 mg/kg Hg for 4 h. We also analyzed the expression levels of NAC transcription factors and found that AsNAC11 had higher expression at 46,000 mg/kg at 2 h; AsNAC17 was underexpressed and the maximum was at 2 h at 23,000 mg/kg. AsNAC20 had the highest expression (30 times more than the control) at 3 and 4 h with 23,000 mg/Kg. AsNAC27 showed the highest expression at 3 h with 23,000 mg/kg. The tissues exhibited a maximum Hg bioconcentration factor of 0.037 at 23,000 mg/kg, indicating moderate mercury absorption. However, at a concentration of 46,000 mg/kg, the BCF decreased to 0.023. Our in-silico analysis revealed that the analyzed AsNACs are associated with various abiotic stress responses. This study provides valuable insights into genes that could be utilized for genetic improvement to enhance crop resistance to mercury soil contamination.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review of Classical and Rising Approaches the Extraction and Utilization of Marine Collagen. 海洋胶原蛋白提取与利用的经典方法及新兴方法综述。

IF 2.7

BioTech Pub Date : 2025-04-03 DOI: 10.3390/biotech14020026

Cesia Deyanira Gutierrez-Canul, Luis Alfonso Can-Herrera, Emmanuel de Jesús Ramírez-Rivera, Witoon Prinyawiwatkul, Enrique Sauri-Duch, Victor Manuel Moo-Huchin, Emanuel Hernández-Núñez

{"title":"A Review of Classical and Rising Approaches the Extraction and Utilization of Marine Collagen.","authors":"Cesia Deyanira Gutierrez-Canul, Luis Alfonso Can-Herrera, Emmanuel de Jesús Ramírez-Rivera, Witoon Prinyawiwatkul, Enrique Sauri-Duch, Victor Manuel Moo-Huchin, Emanuel Hernández-Núñez","doi":"10.3390/biotech14020026","DOIUrl":"https://doi.org/10.3390/biotech14020026","url":null,"abstract":"This comprehensive review explores the extraction and utilization of marine collagen, a sustainable alternative to traditional mammalian sources. The review covers conventional extraction methods like acid and pepsin solubilization, highlighting their limitations and contributing to the search for improved efficiency and sustainability. It also delves into innovative extraction technologies, such as ultrasound-assisted extraction, deep eutectic solvents, and supercritical carbon dioxide, showing their potential to revolutionize the field. The significance of collagen hydrolysis in generating bioactive peptides with diverse functionalities is also discussed, emphasizing their potential applications in various sectors. By providing an analysis of marine collagen extraction and its implications, this review presents a perspective for leveraging this valuable bioresource, promoting a circular economy, and satisfying the increasing demand for high-quality collagen in diverse industries.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green Biocatalysis of Xylitol Monoferulate: Candida antarctica Lipase B-Mediated Synthesis and Characterization of Novel Bifunctional Prodrug. 单酸木糖醇的绿色生物催化：南极念珠菌脂肪酶b介导的新型双功能前药合成与表征。

IF 2.7

BioTech Pub Date : 2025-04-02 DOI: 10.3390/biotech14020025

Federico Zappaterra, Francesco Presini, Domenico Meola, Chaimae Chaibi, Simona Aprile, Lindomar Alberto Lerin, Pier Paolo Giovannini

{"title":"Green Biocatalysis of Xylitol Monoferulate: Candida antarctica Lipase B-Mediated Synthesis and Characterization of Novel Bifunctional Prodrug.","authors":"Federico Zappaterra, Francesco Presini, Domenico Meola, Chaimae Chaibi, Simona Aprile, Lindomar Alberto Lerin, Pier Paolo Giovannini","doi":"10.3390/biotech14020025","DOIUrl":"https://doi.org/10.3390/biotech14020025","url":null,"abstract":"Natural compounds with significant bioactive properties can be found in abundance within biomasses. Especially prominent for their anti-inflammatory, neuroprotective, antibacterial, and antioxidant activities are cinnamic acid derivatives (CAs). Ferulic acid (FA), a widely studied phenylpropanoid, exhibits a broad range of therapeutic and nutraceutical applications, demonstrating antidiabetic, anticancer, antimicrobial, and hepato- and neuroprotective activities. This research investigates the green enzymatic synthesis of innovative and potentially bifunctional prodrug derivatives of FA, designed to enhance solubility and stability profiles. Selective esterification was employed to conjugate FA with xylitol, a biobased polyol recognized for its bioactive antioxidant properties and safety profile. Furthermore, by exploiting t-amyl alcohol as a green solvent, the enzymatic synthesis of the derivative was optimized for reaction parameters including temperature, reaction time, enzyme concentration, and molar ratio. The synthesized derivative, xylitol monoferulate (XMF), represents a novel contribution to the literature. The comprehensive characterization of this compound was achieved using advanced spectroscopic methods, including 1H-NMR, 13C-NMR, COSY, HSQC, and HMBC. This study represents a significant advancement in the enzymatic synthesis of high-value biobased derivatives, demonstrating increased biological activities and setting the stage for future applications in green chemistry and the sustainable production of bioactive compounds.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144056849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of Non-Conventional Yeasts on Low-Cost Carbon Sources and Valorization of Mizithra Secondary Cheese Whey for Metabolite Production. 低成本碳源的非常规酵母筛选及Mizithra次级奶酪乳清代谢产物的生产

IF 2.7

BioTech Pub Date : 2025-04-01 DOI: 10.3390/biotech14020024

Gabriel Vasilakis, Rezart Tefa, Antonios Georgoulakis, Dimitris Karayannis, Ioannis Politis, Seraphim Papanikolaou

{"title":"Screening of Non-Conventional Yeasts on Low-Cost Carbon Sources and Valorization of Mizithra Secondary Cheese Whey for Metabolite Production.","authors":"Gabriel Vasilakis, Rezart Tefa, Antonios Georgoulakis, Dimitris Karayannis, Ioannis Politis, Seraphim Papanikolaou","doi":"10.3390/biotech14020024","DOIUrl":"https://doi.org/10.3390/biotech14020024","url":null,"abstract":"The production of microbial metabolites such as (exo)polysaccharides, lipids, or mannitol through the cultivation of microorganisms on sustainable, low-cost carbon sources is of high interest within the framework of a circular economy. In the current study, two non-extensively studied, non-conventional yeast strains, namely, Cutaneotrichosporon curvatus NRRL YB-775 and Papiliotrema laurentii NRRL Y-3594, were evaluated for their capability to grow on semi-defined lactose-, glycerol-, or glucose-based substrates and produce value-added metabolites. Three different nitrogen-to-carbon ratios (i.e., 20, 80, 160 mol/mol) were tested in shake-flask batch experiments. Pretreated secondary cheese whey (SCW) was used for fed-batch bioreactor cultivation of P. laurentii NRRL Y-3594, under nitrogen limitation. Based on the screening results, both strains can grow on low-cost substrates, yielding high concentrations of microbial biomass (>20 g/L) under nitrogen-excess conditions, with polysaccharides comprising the predominant component (>40%, w/w, of dry biomass). Glucose- and glycerol-based cultures of C. curvatus promote the secretion of mannitol (13.0 g/L in the case of glucose, under nitrogen-limited conditions). The lipids (maximum 2.2 g/L) produced by both strains were rich in oleic acid (≥40%, w/w) and could potentially be utilized to produce second-generation biodiesel. SCW was nutritionally sufficient to grow P. laurentii strain, resulting in exopolysaccharides secretion (25.6 g/L), along with dry biomass (37.9 g/L) and lipid (4.6 g/L) production.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developments in Extracellular Matrix-Based Angiogenesis Therapy for Ischemic Heart Disease: A Review of Current Strategies, Methodologies and Future Directions. 基于细胞外基质的血管生成治疗缺血性心脏病的进展：当前策略、方法和未来方向的综述。

IF 2.7

BioTech Pub Date : 2025-03-19 DOI: 10.3390/biotech14010023

Jad Hamze, Mark Broadwin, Christopher Stone, Kelsey C Muir, Frank W Sellke, M Ruhul Abid

{"title":"Developments in Extracellular Matrix-Based Angiogenesis Therapy for Ischemic Heart Disease: A Review of Current Strategies, Methodologies and Future Directions.","authors":"Jad Hamze, Mark Broadwin, Christopher Stone, Kelsey C Muir, Frank W Sellke, M Ruhul Abid","doi":"10.3390/biotech14010023","DOIUrl":"https://doi.org/10.3390/biotech14010023","url":null,"abstract":"Ischemic heart disease (IHD) is the leading cause of mortality worldwide, underscoring the urgent need for innovative therapeutic strategies. The cardiac extracellular matrix (ECM) undergoes extreme transformations during IHD, adversely influencing the heart's structure, mechanics, and cellular signaling. Researchers investigating the regenerative capacity of the diseased heart have turned their attention to exploring the modulation of ECM to improve therapeutic outcomes. In this review, we thoroughly examine the current state of knowledge regarding the cardiac ECM and its therapeutic potential in the ischemic myocardium. We begin by providing an overview of the fundamentals of cardiac ECM, focusing on the structural, functional, and regulatory mechanisms that drive its modulation. Subsequently, we examine the ECM's interactions within both chronically ischemic and acutely infarcted myocardium, emphasizing key ECM components and their roles in modulating angiogenesis. Finally, we discuss recent ECM-based approaches in biomedical engineering, focusing on different types of scaffolds as delivery tools and their compositions, and conclude with future directions for therapeutic research. By harnessing the potential of these emerging ECM-based therapies, we aim to contribute to the development of novel therapeutic modalities for IHD.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11940646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying Antimicrobial Agents from Chlorella sorokiniana: A Biotechnological Approach Utilizing Eco-Friendly Extraction and Characterization Methods. 从小球藻中鉴定抗菌药物：采用环保提取和表征方法的生物技术方法。

IF 2.7

BioTech Pub Date : 2025-03-18 DOI: 10.3390/biotech14010022

Elia Lio, Martina Dramis, Gianluca Ottolina, Francesco Secundo

{"title":"Identifying Antimicrobial Agents from Chlorella sorokiniana: A Biotechnological Approach Utilizing Eco-Friendly Extraction and Characterization Methods.","authors":"Elia Lio, Martina Dramis, Gianluca Ottolina, Francesco Secundo","doi":"10.3390/biotech14010022","DOIUrl":"https://doi.org/10.3390/biotech14010022","url":null,"abstract":"Natural compounds are increasingly favored over synthetic ones for their lower environmental impact. However, extraction and characterization processes typically rely on harsh conditions and conventional solvents, which are unsustainable and cause pollution. This study aimed to develop an eco-friendly extraction method to isolate and evaluate the antimicrobial properties of bioactive compounds from Chlorella sorokiniana. Using dimethyl carbonate (DMC), methoxycyclopentane (CPME), and butan-2-one (MEK) as green solvents alongside chloroform as a non-green reference solvent, on both untreated and sodium hydroxide pre-treated microalgae biomass, extract yields of up to 182 ± 27 mg/g DW were obtained using MEK. Extracts from untreated microalgae biomass exhibited lower MIC values compared to those obtained with the same solvent from pre-treated biomass, when tested as antimicrobial agents against Escherichia coli, Bacillus megaterium, and Bacillus subtilis. The lowest MIC value (4.89 ± 0.05 µg/mL) was observed against E. coli using the extract from the untreated microalgae biomass with CPME, which was comparable to the vancomycin control (1.55 ± 0.03 µg/mL). Principal component analysis highlighted correlations between GC-MS-identified compounds and antimicrobial activity. ANOVA and post hoc tests (p < 0.05) confirmed solvent choice, and pre-treatment influenced yield and bioactivity. The results underscore green solvents as sustainable alternatives for extracting bioactive compounds from autotrophic microalgae.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic Characterization of Extremely Antibiotic-Resistant Strains of Pseudomonas aeruginosa Isolated from Patients of a Clinic in Sincelejo, Colombia. 哥伦比亚Sincelejo一家诊所患者中分离的极耐抗生素铜绿假单胞菌的基因组特征

IF 2.7

BioTech Pub Date : 2025-03-16 DOI: 10.3390/biotech14010021

Nerlis Pajaro-Castro, Erick Diaz-Morales, Kenia Hoyos

{"title":"Genomic Characterization of Extremely Antibiotic-Resistant Strains of Pseudomonas aeruginosa Isolated from Patients of a Clinic in Sincelejo, Colombia.","authors":"Nerlis Pajaro-Castro, Erick Diaz-Morales, Kenia Hoyos","doi":"10.3390/biotech14010021","DOIUrl":"https://doi.org/10.3390/biotech14010021","url":null,"abstract":"Pseudomonas aeruginosa is an opportunistic pathogen classified as a priority and a great public health concern; therefore, this research focuses on the genomic characterization of extremely resistant strains of P. aeruginosa isolated from patients in a clinic in Sincelejo, Colombia. Seven strains were analyzed by whole genome sequencing using the Illumina NovaSeq platform, with a focus on the identification of resistance genes and virulence factors through the CARD and VFDB databases. An ANI (Average Nucleotide Identity) analysis was carried out to determine the genetic relationship between the strains, complemented by a phylogenomic analysis to place the strains in different evolutionary clades. The results revealed that six of the strains are of Colombian origin, while one strain (547256) belongs to the high-risk clone ST773, previously unidentified in Colombia. Genome size ranged from 6 to 7.4 Mbp, indicating differences in genetic content among strains. Phylogenomic analysis confirmed that five strains belong to a multidrug-resistant (MDR) group, while one strain (572897) showed high alignment with a laboratory strain, and strain 547256 was not associated with any specific clade. Clinically, 100% of strains showed carbapenem resistance, resistance genes, and virulence factors that make them difficult to treat. This study provides key insights into the genetic diversity and resistance patterns of P. aeruginosa in this region, underscoring the need to monitor high-risk clones and optimize therapeutic strategies.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144052565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using Nano-Luciferase Binary (NanoBiT) Technology to Assess the Interaction Between Viral Spike Protein and Angiotensin-Converting Enzyme II by Aptamers. 利用纳米荧光素酶二元（NanoBiT）技术评估病毒刺突蛋白与血管紧张素转换酶II的核酸适配体相互作用。

IF 2.7

BioTech Pub Date : 2025-03-15 DOI: 10.3390/biotech14010020

Meng-Wei Lin, Cheng-Han Lin, Hua-Hsin Chiang, Irwin A Quintela, Vivian C H Wu, Chih-Sheng Lin

{"title":"Using Nano-Luciferase Binary (NanoBiT) Technology to Assess the Interaction Between Viral Spike Protein and Angiotensin-Converting Enzyme II by Aptamers.","authors":"Meng-Wei Lin, Cheng-Han Lin, Hua-Hsin Chiang, Irwin A Quintela, Vivian C H Wu, Chih-Sheng Lin","doi":"10.3390/biotech14010020","DOIUrl":"https://doi.org/10.3390/biotech14010020","url":null,"abstract":"Nano-luciferase binary technology (NanoBiT)-based pseudoviral sensors are innovative tools for monitoring viral infection dynamics. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects host cells via its trimeric surface spike protein, which binds to the human angiotensin-converting enzyme II (hACE2) receptor. This interaction is crucial for viral entry and serves as a key target for therapeutic interventions against coronavirus disease 2019 (COVID-19). Aptamers, short single-stranded DNA (ssDNA) or RNA molecules, are highly specific, high-affinity biorecognition elements for detecting infective pathogens. Despite their potential, optimizing viral infection assays using traditional protein-protein interaction (PPI) methods often face challenges in optimizing viral infection assays. In this study, we selected and evaluated aptamers for their ability to interact with viral proteins, enabling the dynamic visualization of infection progression. The NanoBiT-based pseudoviral sensor demonstrated a rapid increase in luminescence within 3 h, offering a real-time measure of viral infection. A comparison of detection technologies, including green fluorescent protein (GFP), luciferase, and NanoBiT technologies for detecting PPI between the pseudoviral spike protein and hACE2, highlighted NanoBiT's superior sensitivity and performance, particularly in aptamer selection. This bioluminescent system provides a robust, sensitive, and early-stage quantitative approach to studying viral infection dynamics.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11940275/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144039064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0