BioTech最新文献

{"title":"The Role of Microarray in Modern Sequencing: Statistical Approach Matters in a Comparison Between Microarray and RNA-Seq.","authors":"Isaac D Raplee, Samiksha A Borkar, Li Yin, Guglielmo M Venturi, Jerry Shen, Kai-Fen Chang, Upasana Nepal, John W Sleasman, Maureen M Goodenow","doi":"10.3390/biotech14030055","DOIUrl":"10.3390/biotech14030055","url":null,"abstract":"<p><p>Gene expression analysis is crucial in understanding cellular processes, development, health, and disease. With RNA-seq outpacing microarray as the chosen platform for gene expression, is there space for array data in future profiling? This study involved 35 participants from the Adolescent Medicine Trials Network for HIV/AIDS Intervention protocol. RNA was isolated from whole blood samples and analyzed using both microarray and RNA-seq technologies. Data processing included quality control, normalization, and statistical analysis using non-parametric Mann-Whitney U tests. Differential expression analysis and pathway analysis were conducted to compare the outputs of the two platforms. The study found a high correlation in gene expression profiles between microarray and RNA-seq, with a median Pearson correlation coefficient of 0.76. RNA-seq identified 2395 differentially expressed genes (DEGs), while microarray identified 427 DEGs, with 223 DEGs shared between the two platforms. Pathway analysis revealed 205 perturbed pathways by RNA-seq and 47 by microarray, with 30 pathways shared. Both microarray and RNA-seq technologies provide highly concordant results when analyzed with consistent non-parametric statistical methods. The findings emphasize that both methods are reliable for gene expression analysis and can be used complementarily to enhance the robustness of biological insights.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12285979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring of the Single-Cell Behavior of an <i>Escherichia coli</i> Reporter Strain Producing L-phenylalanine in a Scale-Down Bioreactor by Automated Real-Time Flow Cytometry.","authors":"Prasika Arulrajah, Sophi Katharina Riessner, Anna-Lena Heins, Dirk Weuster-Botz","doi":"10.3390/biotech14030054","DOIUrl":"10.3390/biotech14030054","url":null,"abstract":"<p><p>Large-scale bioprocesses often suffer from spatial heterogeneities, which impact microbial performance and often lead to phenotypic population heterogeneity. To better understand these effects at the single-cell level, this study applied, for the first time, automated real-time flow cytometry (ART-FCM) to monitor L-phenylalanine production with an <i>Escherichia coli</i> triple reporter strain in a fed-batch process with glycerol as the carbon source. The strain was cultivated in both a well-mixed stirred-tank bioreactor (STR) and a scale-down two-compartment bioreactor (TCB), consisting of an STR and a coiled flow inverter (CFI) in bypass, to simulate spatial heterogeneities. ART-FCM enabled autonomous, high-frequency sampling every 20 min, allowing for real-time tracking of fluorescence signals linked to growth (<i>rrnB</i>-mEmerald), oxygen availability (<i>narGHIJ</i>-CyOFP1), and product formation (<i>aroFBL</i>-mCardinal2). The STR exhibited uniform reporter expression and higher biomass accumulation, while the TCB showed delayed product formation and pronounced phenotypic diversification depending on the set mean residence time in the CFI. Single-cell fluorescence distributions revealed that the shorter mean residence time in the CFI resulted in pronounced subpopulation formation, whereas longer exposure attenuated heterogeneity, indicating transcriptional adaptation. This finding highlights a critical aspect of scale-down studies: increased exposure duration to perturbations can enhance population robustness. Overall, this study demonstrates the relevance of ART-FCM, in combination with a multi-reporter strain, as a pioneering tool for capturing dynamic cellular behavior and correlating it to process performance, providing deeper insights into microbial heterogeneity under fluctuating bioprocess conditions.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12285959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the Cytotoxicity of Cu(II), Au(III), and Pd(II) Complexes with 2,4-Dithiouracil and 6-Propyl-2-thiouracil Derivatives.","authors":"Petya Marinova, Denica Blazheva, Aleksandar Slavchev, Petia Genova-Kalou","doi":"10.3390/biotech14030053","DOIUrl":"10.3390/biotech14030053","url":null,"abstract":"<p><p>This study investigates the cytotoxic properties of metal complexes incorporating thio-uracil derivatives, specifically 2,4-dithiouracil and 6-propyl-2-thiouracil. The research focuses on the cytotoxic effects of Cu(II) and Pd(II) complexes with 6-propyl-2-thiouracil, as well as mixed-ligand transition metal Cu(II) and Au(III) complexes of 2,4-dithiouracil with 2-thiouracil and uracil. Cytotoxic activity was assessed against human cervical carcinoma cells (HeLa) and normal kidney cells from the African green monkey. The results demonstrated that incorporating Cu(II) and Au(III) into the compound structures significantly enhanced their cytotoxic effects. Notably, all tested complexes exhibited a stronger inhibitory effect on cancer cell proliferation compared to normal cells, with the palladium(II) complex of 6-propyl-2-thiouracil showing the lowest CD₅₀ value against the tumor cell line (0.00064 mM), which were 149 times lower than that of the ligand (0.0955 mM). These findings suggest that thio-uracil-based metal complexes, particularly those containing palladium (II) and gold(III), hold significant potential for further development as anticancer agents.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12285946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reactive Molecules in Cigarette Smoke: Rethinking Cancer Therapy.","authors":"Vehary Sakanyan","doi":"10.3390/biotech14030052","DOIUrl":"10.3390/biotech14030052","url":null,"abstract":"<p><p>Science has made significant progress in detecting reactive oxygen species (ROS) in tobacco smoke, which is an important step for precision cancer therapy. An important advance is also the understanding that superoxide can be produced by electrophilic molecules. The dual action of hydrogen peroxide, directly or via electrophilic molecules, in the development of oxidative stress allows for the identification of target proteins that can potentially stop unwanted signals in cancer development. However, despite advances in proteomics, reliable inhibitors to stop ROS-associated cancer progression have not yet been proposed for the treatment of tobacco cigarette smokers. This is likely due to an imperfect understanding of the diversity of molecular mechanisms of anti-ROS action. Fluorescent protein detection in living cells, called in-gel, offers a direct route to a better understanding of the rapid interaction of ROS and electrophilic compounds with targeted proteins. It seemed that the traditional paradigm of pharmaceutical innovation \"one drug, one disease\" did not solve the problem of tobacco smoking causing cancer. However, among the various therapeutic treatments for tobacco smokers, the best way to combat cancer today is smoking cessation, which fits into the \"one-cure\" paradigm.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12286076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and Biological Activity Analysis of Recombinant Fibronectin3 Protein in <i>Bacillus subtilis</i>.","authors":"Chaozheng Lu, Guangxin Xu, Yin Tian, Zhiwei Yi, Xixiang Tang","doi":"10.3390/biotech14030051","DOIUrl":"10.3390/biotech14030051","url":null,"abstract":"<p><p>Fibronectin (FN), a primary component of the extracellular matrix (ECM), features multiple structural domains closely linked to various cellular behaviors, including migration, spreading, adhesion, and proliferation. The FN3 domain, which contains the RGD sequence, is critical in tissue repair because it enables interaction with integrin receptors on the cell surface. However, the large molecular weight of wild-type FN presents challenges for its large-scale production through heterologous expression. Therefore, this study focused on cloning the FN3 functional domain of full-length FN for expression and validation. This study selected <i>Bacillus subtilis</i> as the expression host due to its prominent advantages, including efficient protein secretion, absence of endotoxins, and minimal codon bias. The recombinant vector pHT43-FN3 was successfully constructed through homologous recombination technology and transformed into <i>Bacillus subtilis</i> WB800N. The FN3 protein was successfully expressed after induction with IPTG. Following purification of the recombinant FN protein using a His-tag nickel column, SDS-PAGE analysis showed that the molecular weight of FN3 was approximately 27.3 kDa. Western blot analysis confirmed the correct expression of FN3, and the BCA protein assay kit determined a protein yield of 5.4 mg/L. CCK8 testing demonstrated the good biocompatibility of FN3. In vitro cell experiments showed that FN3 significantly promoted cell migration at a 20 μg/mL concentration and enhanced cell adhesion at 10 μg/mL. In summary, this study successfully utilized <i>Bacillus subtilis</i> to express the FN3 functional domain peptide from FN protein and has validated its ability to promote cell migration and adhesion. These findings not only provide a strategy for the expression of FN protein in <i>B. subtilis</i>, but also establish an experimental foundation for the potential application of FN3 protein in tissue repair fields such as cutaneous wound healing and cartilage regeneration.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12286206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Halotolerant Bacteria from Saline Environments: Isolation and Biomolecule Production.","authors":"Simona Neagu, Mihaela Marilena Stancu","doi":"10.3390/biotech14020049","DOIUrl":"10.3390/biotech14020049","url":null,"abstract":"<p><p>Microorganisms from saline environments have garnered significant interest due to their unique adaptations, which enable them to thrive under high-salt conditions and synthesize valuable biomolecules. This study investigates the biosynthesis of biomolecules, such as extracellular hydrolytic enzymes, biosurfactants, and carotenoid pigments, by four newly halotolerant bacterial strains isolated from saline environments in the Băicoi (soil, water) and Curmătura (mud) area (Prahova County, Romania). Isolation was performed on two selective culture media with different NaCl concentrations (1.7 M, 3.4 M). Based on their phenotypic and molecular characteristics, the four halotolerant bacteria were identified as <i>Halomonas elongata</i> SB8, <i>Bacillus altitudinis</i> CN6, <i>Planococcus rifietoensis</i> CN8, and <i>Halomonas stenophila</i> IB5. The two bacterial strains from the <i>Halomonas</i> genus exhibited growth in MH medium containing elevated NaCl concentrations (0-5 M), in contrast to the other two strains from <i>Bacillus</i> (0-2 M) and <i>Planococcus</i> (0-3 M). The growth of these bacteria under different salinity conditions, hydrocarbon tolerance, and biomolecule production were assessed through biochemical assays, spectrophotometry, and high-performance thin-layer chromatography. The antimicrobial properties of biosurfactants and carotenoids produced by <i>H. elongata</i> SB8, <i>B. altitudinis</i> CN6, <i>P. rifietoensis</i> CN8, and <i>H. stenophila</i> IB5 were evaluated against four reference pathogenic microorganisms from the genera <i>Escherichia</i>, <i>Pseudomonas</i>, <i>Staphylococcus</i>, and <i>Candida</i>. <i>H. elongata</i> SB8 showed the highest hydrocarbon tolerance. <i>B. altitudinis</i> CN6 exhibited multiple hydrolase activities and, along with <i>H. elongata</i> SB8, demonstrated biosurfactant production. <i>P. rifietoensis</i> CN8 produced the highest carotenoid concentration with antifungal and antimicrobial activity. Exploring these organisms opens new pathways for bioremediation, industrial bioprocessing, and sustainable biomolecule production.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12191407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leaf Organogenesis Improves Recovery of Solid Polyploid Shoots from Chimeric Southern Highbush Blueberry.","authors":"Emily Walter, Akshaya Biswal, Peggy Ozias-Akins, Ye Chu","doi":"10.3390/biotech14020048","DOIUrl":"10.3390/biotech14020048","url":null,"abstract":"<p><p>Interspecific and intersectional crosses have introduced valuable genetic traits for blueberry (<i>Vaccinium</i> sect. <i>Cyanococcus</i>) cultivar improvement. Introgression from <i>Vaccinium</i> species at the diploid, tetraploid, and hexaploid levels has been found in cultivated blueberries. Continued efforts to integrate wild blueberry genetic resources into blueberry breeding are essential to broaden the genetic diversity of cultivated blueberries. However, performing heteroploid crosses among <i>Vaccinium</i> species is challenging. Polyploid induction through tissue culture has been useful in bridging ploidy barriers. Mixoploid or chimeric shoots often are produced, along with solid polyploid mutants. These chimeras are mostly discarded because of their genome instability and the difficulty in identifying periclinal mutants carrying germline mutations. Since induced polyploidy in blueberries often results in a low frequency of solid mutant lines, it is important to recover solid polyploids through chimera dissociation. In this study, two vegetative propagation methods, i.e., axillary and adventitious shoot induction, were evaluated for their efficiency in chimera dissociation. Significantly higher rates of chimera dissociation were found in adventitious shoot induction compared to axillary shoot induction. Approximately 89% and 82% of the adventitious shoots induced from mixoploid lines 145.11 and 169.40 were solid polyploids, respectively, whereas only 25% and 53% of solid polyploids were recovered through axillary shoot induction in these lines. Effective chimera dissociation provides useful and stable genetic materials to enhance blueberry breeding.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12190331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Inflammatory Function Analysis of <i>Lacticaseibacillus rhamnosus</i> CP-1 Strain Based on Whole-Genome Sequencing.","authors":"Hanyu Chu, Lijie Zhou, Yanzhen Mao, Ren Liu, Jiaojiao Han, Xiurong Su, Jun Zhou","doi":"10.3390/biotech14020047","DOIUrl":"10.3390/biotech14020047","url":null,"abstract":"<p><p><i>Lacticaseibacillus rhamnosus</i> (<i>L. rhamnosus</i>) is a safe probiotic with no side effects, providing benefits such as gut microbiota regulation and immune enhancement, making it highly valuable with strong potential. However, strains from different sources have unique traits, and whole-genome sequencing (WGS) helps analyse these differences. In this study, we used WGS to examine <i>L. rhamnosus</i> strains from mice with fish oil-treated smoking-induced pneumonia to better understand their biological functions and explore possible anti-inflammatory mechanisms.</p><p><strong>Methods: </strong>We isolated a strain, <i>Lacticaseibacillus rhamnosus</i> CP-1 (<i>L. rhamnosus</i> CP-1), from mice intestines where fish oil alleviated smoking-induced pneumonia. Identification of probiotic-related genes by WGS and characterised the strain's probiotic properties.</p><p><strong>Results: </strong><i>L. rhamnosus</i> CP-1 has a single circular chromosome (2,989,570 bp, 46.76% GC content) and no plasmids. COG, GO, and KEGG databases revealed genes linked to carbohydrate metabolism. The CAZy database identified GH25 lysozyme and PL8 polysaccharide lyase genes. KEGG highlighted an antimicrobial peptide ABC transporter permease, while TCDB noted the ABC-type antimicrobial peptide transporter (the main active transport component). KEGG also showed 10 genes for terpenoid skeleton biosynthesis and 5 for keto-glycan unit biosynthesis. Additionally, <i>L. rhamnosus</i> CP-1 carries metabolic regulators and bacteriocin-related genes.</p><p><strong>Conclusions: </strong>Whole-genome sequencing analysis revealed that <i>L. rhamnosus</i> CP-1 has carbohydrate utilisation and potential anti-inflammatory effects at the molecular level. Potential functional genes include carbohydrate transport and hydrolase, antimicrobial peptide ABC transporter and its osmotic enzyme components, bacteriocin immune protein, terpenoid skeleton, and keto-glycan synthesis.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12190639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green-Synthesized Silver Nanoparticles (AgNPs) Enhance In Vitro Multiplication and Rooting of Strawberries (<i>Fragaria</i> × <i>ananassa</i> Duchesne).","authors":"José Luis Aguirre-Noyola, Marco A Ramírez-Mosqueda, Jorge David Cadena-Zamudio, José Humberto Caamal-Velázquez, Esmeralda J Cruz-Gutiérrez, Alma Armenta-Medina","doi":"10.3390/biotech14020045","DOIUrl":"10.3390/biotech14020045","url":null,"abstract":"<p><p>Nanobiotechnology applications in plant tissue culture have improved the development and physiology of explants, resulting in plants with high genetic homogeneity and phytosanitary quality. Silver nanoparticles (AgNPs) are well-known for their microbicidal properties, but their biochemical effects on plants require further exploration. In this work, green-synthesized AgNPs were evaluated in strawberry in vitro culture, photosynthetic pigment production, and acclimatization. AgNPs produced by <i>Lysinibacillus fusiformis</i> were characterized. Strawberry explants were grown in vitro on MS medium with 0, 100, 200, and 300 mg L^-1 AgNPs at 24 ± 2 °C and a photoperiod of 16:8 h light/dark. Shoot height and number, number of leaves, number of roots, and root length were evaluated, and chlorophyll (a, b, and total) was quantified. Rooted shoots were acclimatized ex vitro on substrates containing 0 and 200 mg L^-1 AgNPs. The results showed that low AgNPs concentrations had a positive impact on shoot multiplication, development, and rooting, but at higher concentrations, the effects decayed. However, chlorophyll production improved with increasing AgNP concentration. Shoots treated with AgNPs showed higher ex vitro survival. Our study has direct implications for the profitability and sustainability of commercial strawberry production.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12191388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Antioxidant Potential, Antidiabetic Activities, and GC-MS Analysis of Lipid Extracts of <i>Chlorella</i> Microalgae.","authors":"Somruthai Kaeoboon, Rattanaporn Songserm, Rungcharn Suksungworn, Sutsawat Duangsrisai, Nuttha Sanevas","doi":"10.3390/biotech14020046","DOIUrl":"10.3390/biotech14020046","url":null,"abstract":"<p><p>Microalgae represent promising biotechnological platforms for bioactive compound production with pharmaceutical applications. This study investigated the phytochemical composition and biological activities of lipid extracts from three <i>Chlorella</i> species to evaluate their potential as antioxidant and antidiabetic sources. Lipid extraction using chloroform-methanol (2:1) followed by GC-MS analysis revealed distinct compound distributions: 29 compounds in <i>C. ellipsoidea</i>, 33 in <i>C. sorokiniana</i>, and 19 in <i>C. vulgaris</i>. Major bioactive compounds included 2-hexanol, 1,3,6-heptatriene, 4-(2,3-dimethyl-2-cyclopenten-1-yl)-4-methylpentanal, <i>n</i>-hexadecanoic acid, and octadecanoic acid. Biological activity screening encompassed antioxidant assessment through DPPH• and •NO radical scavenging assays and FRAP analysis, while antidiabetic potential was evaluated using α-glucosidase and α-amylase inhibition assays. <i>C. sorokiniana</i> exhibited superior bioactivity with the highest antioxidant capacity (DPPH• IC₅₀ = 329.03 ± 4.30 µg/mL; •NO IC₅₀ = 435.53 ± 10.20 µg/mL; FRAP = 94.74 ± 5.72 mg TE/g) and strongest enzyme inhibition (α-glucosidase IC₅₀ = 752.75 ± 57.95 µg/mL; α-amylase IC₅₀ = 3458.50 ± 104.01 µg/mL). This is the first report on <i>C. sorokiniana</i> strain KU.B2's biological properties and phytochemical profile. These findings establish <i>C. sorokiniana</i> as a valuable biotechnological platform for pharmaceutical bioactive compound development.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12190495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}