BioTech最新文献 - Book学术

Innovative Approaches to EMT-Related Biomarker Identification in Breast Cancer: Multi-Omics and Machine Learning Methods. 乳腺癌emt相关生物标志物鉴定的创新方法：多组学和机器学习方法。

IF 3.1

BioTech Pub Date : 2025-09-22 DOI: 10.3390/biotech14030075

Ghazaleh Khalili-Tanha, Alireza Shoari

引用次数: 0

Bacillus Pectinases as Key Biocatalysts for a Circular Bioeconomy: From Green Extraction to Process Optimization and Industrial Scale-Up. 果胶芽孢杆菌酶作为循环生物经济的关键生物催化剂：从绿色提取到工艺优化和工业规模化。

IF 3.1

BioTech Pub Date : 2025-09-19 DOI: 10.3390/biotech14030074

Fatima Zohra Kaissar, Khelifa Bouacem, Mohammed Lamine Benine, Sondes Mechri, Shubha Rani Sharma, Vishal Kumar Singh, Mahfoud Bakli, Seif El Islam Lebouachera, Giovanni Emiliani

{"title":"Bacillus Pectinases as Key Biocatalysts for a Circular Bioeconomy: From Green Extraction to Process Optimization and Industrial Scale-Up.","authors":"Fatima Zohra Kaissar, Khelifa Bouacem, Mohammed Lamine Benine, Sondes Mechri, Shubha Rani Sharma, Vishal Kumar Singh, Mahfoud Bakli, Seif El Islam Lebouachera, Giovanni Emiliani","doi":"10.3390/biotech14030074","DOIUrl":"10.3390/biotech14030074","url":null,"abstract":"Pectins are high-value plant cell-wall polysaccharides with extensive applications in the food, pharmaceutical, textile, paper, and environmental sectors. Traditional extraction and processing methodologies rely heavily on harsh acids, high temperatures, and non-renewable solvents, generating substantial environmental and economic costs. This review consolidates recent advances across the entire Bacillus-pectinase value chain, from green pectin extraction and upstream substrate characterization, through process and statistical optimization of enzyme production, to industrial biocatalysis applications. We propose a practical roadmap for developing high-efficiency, low-environmental-footprint enzyme systems that support circular bioeconomy objectives. Critical evaluation of optimization strategies, including submerged versus solid-state fermentation, response surface methodology, artificial neural networks, and design of experiments, is supported by comparative data on strain performance, fermentation parameters, and industrial titers. Sector-specific case studies demonstrate the efficacy of Bacillus pectinases in fruit-juice clarification, textile bio-scouring, paper bio-bleaching, bio-based detergents, coffee and tea processing, oil extraction, animal feed enhancement, wastewater treatment, and plant-virus purification. Remaining challenges, including enzyme stability in complex matrices, techno-economic scale-up, and structure-guided protein engineering, are identified. Future directions are charted toward CRISPR-driven enzyme design and fully integrated circular-economy bioprocessing platforms.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-Based Pipeline for Plant Enzymes: Pilot Study Identifying Novel Ginsenoside Biosynthetic UGTs. 基于结构的植物酶管道：鉴定新型人参皂苷生物合成UGTs的初步研究。

IF 3.1

BioTech Pub Date : 2025-09-12 DOI: 10.3390/biotech14030073

Kisook Jung, Ick-Hyun Jo, Bae Young Choi, Jaewook Kim

{"title":"Structure-Based Pipeline for Plant Enzymes: Pilot Study Identifying Novel Ginsenoside Biosynthetic UGTs.","authors":"Kisook Jung, Ick-Hyun Jo, Bae Young Choi, Jaewook Kim","doi":"10.3390/biotech14030073","DOIUrl":"10.3390/biotech14030073","url":null,"abstract":"Models that predict the 3D structure of proteins enable us to easily analyze the structure of unknown proteins. Though many of these models have been found to be accurate, their application in plant proteins is not always entirely accurate. Thus, we aimed to develop a versatile yet simple pipeline that can predict novel proteins with a specific function. As an example, via benchmark studies, we sought to discover novel UDP-glycosyltransferases (UGTs) potentially involved in ginsenoside biosynthesis. Since the functionality of these UGTs has been shown to be determined by a few amino acids, a 3D-structure-based pipeline was required. Our pipeline includes four sequential steps: a sequence-based homology search, AlphaFold3-based 3D structure prediction, docking simulations with ginsenoside intermediates using SwissDock and CB-Dock2, and MPEK analysis to assess interaction stability. Through the application of this benchmark, we optimized the role of each module in the pipeline and successfully identified four novel UGT candidates. These candidates are predicted to catalyze the conversion of protopanaxadiol (PPD) to compound K (CK) or protopanaxatriol (PPT) to ginsenoside F1. This pilot study demonstrates how our pipeline can be used for the functional annotation of plant proteins and the discovery of enzymes involved in specialized pathways.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Benchmarking the Base Randomization Algorithm as a Possible Tool for the Initial Step of Generating a Virtual RNA Aptamers Library. 基准随机化算法作为生成虚拟RNA适体库初始步骤的可能工具。

IF 3.1

BioTech Pub Date : 2025-09-12 DOI: 10.3390/biotech14030072

Kabelo P Mokgopa, Shina D Oloniiju, Kevin A Lobb, Tendamudzimu Tshiwawa

{"title":"Benchmarking the Base Randomization Algorithm as a Possible Tool for the Initial Step of Generating a Virtual RNA Aptamers Library.","authors":"Kabelo P Mokgopa, Shina D Oloniiju, Kevin A Lobb, Tendamudzimu Tshiwawa","doi":"10.3390/biotech14030072","DOIUrl":"10.3390/biotech14030072","url":null,"abstract":"While databases are emerging across various domains, from small molecules to genomics and proteins, aptamer databases remain scarce, if not entirely absent. Such databases could serve as a comprehensive resource for advancing research, innovation, and the applications of aptamer technology across multiple fields. This advancement would likely lead to improvements in healthcare, environmental monitoring, and biotechnology. Furthermore, the establishment of aptamer databases would facilitate molecular modelling and machine learning, opening doors to further advancements in understanding and utilizing aptamers. Against this backdrop, in this study, we present and benchmark the Base Randomization Algorithm (BRA) as a potential solution to the scarcity of aptamer databases. Through statistical analysis, we examine key factors such as minimum free energy (MFE), base compositions, and base arrangements. Notably, sequences generated using the BRA exhibit a Gaussian distribution pattern. We also examine the details of how each base within a sequence is chosen using mathematical principles, ensuring that the sequences are valid and optimized statistically. Additionally, we explore how the length of the randomized generated sequences can affect the folding of their structures at both the secondary and tertiary levels. Based on composition analysis, we propose that the base mean of the dataset can be approximated as x¯B≈Px × N, for dataset of sequences with the same length and x¯B≈Px × M, where M is the median and N the mean, for a dataset with randomized length that follows a Gaussian distribution.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Role of Tenebrio molitor Frass in Sustainable Agriculture: Effects on Free-Living Nematodes and Suppression of Meloidogyne incognita. 黄粉甲在可持续农业中的双重作用：对游离线虫的抑制作用和对隐棉线虫的抑制作用。

IF 3.1

BioTech Pub Date : 2025-09-08 DOI: 10.3390/biotech14030071

Evgenia Rizou, Nikolaos Monokrousos, Triantafyllia Kardami, Georgia V Baliota, Christos I Rumbos, Christos G Athanassiou, Nikolaos Tsiropoulos, Nikoletta Ntalli

{"title":"Dual Role of Tenebrio molitor Frass in Sustainable Agriculture: Effects on Free-Living Nematodes and Suppression of Meloidogyne incognita.","authors":"Evgenia Rizou, Nikolaos Monokrousos, Triantafyllia Kardami, Georgia V Baliota, Christos I Rumbos, Christos G Athanassiou, Nikolaos Tsiropoulos, Nikoletta Ntalli","doi":"10.3390/biotech14030071","DOIUrl":"10.3390/biotech14030071","url":null,"abstract":"Insect-derived frass is gaining attention as a circular bioeconomy product with fertilizing and pest-suppressive potential. This study investigates Tenebrio molitor frass as a soil amendment for promoting beneficial nematodes and suppressing Meloidogyne incognita. A 40-day pot experiment on clay loam soil tested with six inputs: raw and heat-treated frass (0.5%, 1% w/w), Melia azedarach fruit powder (1.6%), and an untreated control. Soil nematode communities were assessed at 5 and 40 days after application (DAA), and nematicidal activity was evaluated in vitro. Raw frass at 1% induced a rapid response from free-living nematodes at 5 DAA, with increased abundance of bacterivorous taxa such as Rhabditis and Acrobeloides, alongside a higher Enrichment Index (EI), indicating short-term nutrient availability. At 40 DAA, only 1% raw frass consistently supported more cp-1 bacterivores and slightly increased Shannon diversity. Network analysis revealed more connected, modular structures in raw frass treatments, suggesting enhanced food web complexity. However, omnivore and predator effects were limited. Raw frass extracts caused over 80% paralysis of Meloidogyne incognita juveniles within 24 h, significantly outperforming heat-treated frass and Melia extracts. T. molitor frass moderately stimulates opportunistic nematodes and provides strong nematicidal effects, supporting its potential as a multifunctional input for sustainable soil management.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452486/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Single-Nucleus Transcriptomic Atlas of the Mouse Lumbar Spinal Cord: Functional Implications of Non-Coding RNAs. 小鼠腰脊髓单核转录组图谱：非编码rna的功能意义。

IF 3.1

BioTech Pub Date : 2025-09-03 DOI: 10.3390/biotech14030070

Pablo Ruiz-Amezcua, Miguel Nieto Hernández, Javier García Flores, Clara Plaza Alonso, David Reigada, Teresa Muñoz-Galdeano, Eva Vargas, Rodrigo M Maza, Francisco J Esteban, Manuel Nieto-Díaz

{"title":"A Single-Nucleus Transcriptomic Atlas of the Mouse Lumbar Spinal Cord: Functional Implications of Non-Coding RNAs.","authors":"Pablo Ruiz-Amezcua, Miguel Nieto Hernández, Javier García Flores, Clara Plaza Alonso, David Reigada, Teresa Muñoz-Galdeano, Eva Vargas, Rodrigo M Maza, Francisco J Esteban, Manuel Nieto-Díaz","doi":"10.3390/biotech14030070","DOIUrl":"10.3390/biotech14030070","url":null,"abstract":"The adult lumbar spinal cord plays a critical role in locomotor control and somatosensory integration, whose transcriptional architecture under physiological conditions has been characterized in various studies with restricted numbers of individuals (up to four). Here, we present an integrative single-nucleus RNA sequencing (snRNA-seq) atlas of the healthy adult mouse lumbar spinal cord, assembled from over 86,000 nuclei from 16 samples across five public datasets. Using a harmonized computational pipeline, we identify all major spinal cell lineages and resolve 17 transcriptionally distinct neuronal subtypes. A central novelty of our approach is the systematic inclusion of non-coding RNAs (ncRNAs), including long non-coding RNAs (lncRNAs) and pseudogenes. By comparing transcriptomic analyses based on coding-only, non-coding-only, and combined gene sets, we show that ncRNAs, despite accounting to a 10% of the recorded information of each cell, contribute to cell type-specific signatures. This resource offers a high-resolution, ncRNA-inclusive reference for the adult spinal cord and provides a foundation for future studies on spinal plasticity, injury, and regeneration.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organic Acid Production by Basfia succiniciproducens from Agro-Industrial By-Products. 农工副产品琥珀酸Basfia琥珀酸生产有机酸的研究。

IF 3.1

BioTech Pub Date : 2025-09-01 DOI: 10.3390/biotech14030068

Márta Balázs, Izabella Péter, Hunor Bartos, Zsolt Bodor, Emőke Antal, Csilla Albert, Ildikó Miklóssy

{"title":"Organic Acid Production by Basfia succiniciproducens from Agro-Industrial By-Products.","authors":"Márta Balázs, Izabella Péter, Hunor Bartos, Zsolt Bodor, Emőke Antal, Csilla Albert, Ildikó Miklóssy","doi":"10.3390/biotech14030068","DOIUrl":"10.3390/biotech14030068","url":null,"abstract":"A continuous effort is needed to develop sustainable production methods for industrial platform chemicals. B. succiniciproducens, a natural succinic acid-producer, can metabolize five and six carbon atoms containing sugars in pure form as well as from agro-industrial wastes. In our work, we investigate the conversion of industrial by-products, apple pomace from apple juice production, and whey waste from milk processing to succinic acid and other organic acids (lactic, formic, and acetic acid). We obtained a succinic acid yield of 0.224 g/g total consumed fermentable sugars, lactic acid yield was 0.087 g/g, in turn, formic acid was produced at a 0.034 g/g yield, and acetic acid was obtained at 0.010 g/g total consumed fermentable sugars, using a thermal pretreated apple pomace-based medium. In the case of pretreated whey-based medium formulation, we obtained a succinic acid yield of 0.236 g/g consumed lactose, while formic acid and acetic acid were produced as well (0.09 g/g and 0.101 g/g, respectively). We demonstrate that lactose is a promising carbon source for organic acid production by B. succiniciproducens, while our study is the first to propose the use of a similarly available agro-industrial by-product, apple pomace, for the fermentative production of succinic acid by B. succiniciproducens.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Selected Truffle-Associated Bacteria and Fungi on the Mycorrhization of Quercus ilex Seedlings with Tuber melanosporum. 松露伴生细菌和真菌对黑孢块茎栎树幼苗菌根化的影响。

IF 3.1

BioTech Pub Date : 2025-09-01 DOI: 10.3390/biotech14030069

Eva Gómez-Molina, Pedro Marco, Sergi Garcia-Barreda, Vicente González, Sergio Sánchez

{"title":"Effect of Selected Truffle-Associated Bacteria and Fungi on the Mycorrhization of Quercus ilex Seedlings with Tuber melanosporum.","authors":"Eva Gómez-Molina, Pedro Marco, Sergi Garcia-Barreda, Vicente González, Sergio Sánchez","doi":"10.3390/biotech14030069","DOIUrl":"10.3390/biotech14030069","url":null,"abstract":"The success of truffle cultivation is especially dependent on the quality of truffle-mycorrhized seedlings, which are typically produced in nurseries under aseptic conditions to avoid root colonization by undesired ectomycorrhizal fungi. However, such practices may also eliminate beneficial microorganisms that could support truffle symbiosis and improve seedling quality. In this study, twelve endophytic bacterial and fungal strains, isolated from the Tuber melanosporum environment (gleba tissue, mycorrhizae and truffle brûlé), were tested for their effect on T. melanosporum mycorrhization levels in inoculated Quercus ilex seedlings under nursery conditions. Co-inoculation with a strain of Agrobacterium tumefaciens significantly enhanced root colonization by T. melanosporum, supporting its potential role as mycorrhizal helper bacterium. In contrast, a strain of Trichoderma harzianum negatively affected mycorrhization. The remaining strains did not show significant effects on seedling mycorrhization or seedling growth. Our findings support the hypothesis that specific bacterial strains associated with truffles can act as mycorrhizal helper bacteria, highlighting the potential for co-inoculation strategies to enhance quality of truffle-inoculated seedlings in nurseries. However, further research is needed to gain a deeper understanding of the interactions within the mycorrhizosphere that could contribute to improving nursery seedling quality.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in Microbial Communities in Industrial Anaerobic Digestion of Dairy Manure Caused by Caldicellulosiruptor Pretreatment. 钙纤维素裂解剂预处理对奶牛粪便工业厌氧消化微生物群落的影响。

IF 3.1

BioTech Pub Date : 2025-08-28 DOI: 10.3390/biotech14030067

Jakob Young, Maliea Nipko, Spencer Butterfield, Zachary Aanderud

{"title":"Changes in Microbial Communities in Industrial Anaerobic Digestion of Dairy Manure Caused by Caldicellulosiruptor Pretreatment.","authors":"Jakob Young, Maliea Nipko, Spencer Butterfield, Zachary Aanderud","doi":"10.3390/biotech14030067","DOIUrl":"10.3390/biotech14030067","url":null,"abstract":"Extremophilic biological process (EBP) pretreatment increases substrate availability in anaerobic digestion, but the effect on downstream microbial community composition in industrial systems is not characterized. Changes in microbial communities were determined at an industrial facility processing dairy manure in a modified split-stream system with three reactor types: (1) EBP tanks at 70-72 °C; (2) mesophilic Continuously Stirred Tank Reactors (CSTRs); (3) mesophilic Induced Bed Reactors (IBRs) receiving combined CSTR and EBP effluent. All reactors had a two-day hydraulic retention time. Samples were collected weekly for 60 days. pH, volatile fatty acid and bicarbonate concentrations, COD, and methane yield were measured to assess tank environmental conditions. Microbial community compositions were obtained via 16S rRNA gene sequencing. EBP pretreatment increased acetate availability but led to a decline in the relative abundance of acetoclastic Methanosarcina species in downstream IBRs. Rather, syntrophic methanogens, e.g., members of Methanobacteriaceae, increased in relative abundance and became central to microbial co-occurrence networks, particularly in association with hydrogen-producing bacteria. Network analysis also demonstrated that these syntrophic relationships were tightly coordinated in pretreated digestate but absent in the untreated CSTRs. By promoting syntrophic methanogenesis while increasing acetate concentrations, EBP pretreatment requires system configurations that enable acetoclast retention to prevent acetate underutilization and maximize methane yields.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Bacterial Cellulose Production Using Hempseed Meal: Optimal Conditions and Properties. 利用大麻籽粕增强细菌纤维素生产：最佳条件和性能。

IF 3.1

BioTech Pub Date : 2025-08-27 DOI: 10.3390/biotech14030066

Sawichaya Orpool, Suthaphat Kamthai, Thanyaporn Siriwoharn, Patompong Khaw-On, Aree Deenu, Srisuwan Naruenartwongsakul

{"title":"Enhanced Bacterial Cellulose Production Using Hempseed Meal: Optimal Conditions and Properties.","authors":"Sawichaya Orpool, Suthaphat Kamthai, Thanyaporn Siriwoharn, Patompong Khaw-On, Aree Deenu, Srisuwan Naruenartwongsakul","doi":"10.3390/biotech14030066","DOIUrl":"10.3390/biotech14030066","url":null,"abstract":"Hemp (Cannabis sativa L.) seed is progressively emerging as an innovative and sustainable source of plant oil. Defatted hempseed meal is rich in protein and carbohydrates, which bacteria can convert into cellulose using glucose and fructose. The optimal conditions for bacterial cellulose (BC) production from hempseed meal were evaluated by investigating total solid concentrations ranging from 8 to 16 °Brix using Komagataeibacter nataicola under controlled conditions. The changes in pH, bioactive compounds, organic acids, and carbon source concentrations were monitored during the fermentation process. The highest yield of BC, 12.41 g/L, was obtained at 10 °Brix after 14 days of fermentation. It was found that the production of BC was negatively impacted by a decrease in pH and an increase in organic acids. BC exhibited a ribbon-like 3D network structure and a crystallinity index of about 70%, with excellent water-holding capacity, low oil-holding capacity, high emulsifying activity, and high emulsion stability (11.21%, 2.71%, 34.33%, and 39.11%, respectively). This BC possesses exceptional mechanical properties, a high degree of crystallinity, and superior water-holding capacity, making it valuable in various industries such as food, pharmaceuticals, and biotechnology.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0