BioTech最新文献 - Book学术

Functional Bio-Based Additives for Sustainable Polymers: A Systematic Review of Processing and Performance Enhancers. 可持续聚合物的功能性生物基添加剂：加工和性能增强剂的系统综述。

IF 3.1

BioTech Pub Date : 2026-04-22 DOI: 10.3390/biotech15020031

Odilon Souza Leite-Barbosa, Debora Cristina da Silva Santos, Cláudia Carnaval de Oliveira Pinto, Fernanda Cristina Fernandes Braga, Marcia Gomes de Oliveira, Marcelo Ferreira Leão de Oliveira, Valdir Florêncio da Veiga-Junior

{"title":"Functional Bio-Based Additives for Sustainable Polymers: A Systematic Review of Processing and Performance Enhancers.","authors":"Odilon Souza Leite-Barbosa, Debora Cristina da Silva Santos, Cláudia Carnaval de Oliveira Pinto, Fernanda Cristina Fernandes Braga, Marcia Gomes de Oliveira, Marcelo Ferreira Leão de Oliveira, Valdir Florêncio da Veiga-Junior","doi":"10.3390/biotech15020031","DOIUrl":"https://doi.org/10.3390/biotech15020031","url":null,"abstract":"Background: The transition from fossil-derived polymer additives to renewable alternatives is essential to mitigate environmental persistence and ensure chemical safety within the plastics industry. This review provides a comprehensive overview of recent developments in bio-based functional additives and their integration into circular economy frameworks. Methods: Following PRISMA guidelines, a systematic literature search was conducted using the Scopus database for studies published between 2023 and 2026. Search terms targeted bio-based plasticizers, flame retardants, antioxidants, and compatibilizers. Studies were screened against predefined inclusion criteria, specifically focusing on experimental validation in polymer matrices, while data mining was employed to map emerging research fronts. Results: From an initial 996 records, 54 studies were selected after removing duplicates and ineligible articles. The findings highlight a paradigm shift from passive physical fillers toward active, multifunctional macromolecular agents. Recent literature demonstrates that targeted molecular interventions, such as phosphorylated lignin and biomimetic structures, can resolve trade-offs between ductility and thermal stability at low loadings (<5 wt%). Synthesis routes, performance outcomes, and end-of-life trajectories for each additive class are summarized. Conclusions: Bio-based additives have evolved from simple substitutes into strategic tools for the molecular programming of sustainable polymers. Although challenges regarding scalability and high-temperature processing persist, their integration into circular economy strategies establishes a clear roadmap for next-generation bioplastics.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13108088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147783430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene Flow and Hybridization Potential Between GM/NGT Crops and Conventional Varieties or Wild Relatives: A Scoping Literature Review with Emphasis on Oilseed Rape (Brassica napus L.) and Potato (Solanum tuberosum L.). 转基因/NGT作物与常规品种或野生近缘种的基因流动和杂交潜力——以油菜和马铃薯为重点的文献综述

IF 3.1

BioTech Pub Date : 2026-04-08 DOI: 10.3390/biotech15020030

Lelde Grantina-Ievina, Nils Rostoks

{"title":"Gene Flow and Hybridization Potential Between GM/NGT Crops and Conventional Varieties or Wild Relatives: A Scoping Literature Review with Emphasis on Oilseed Rape (Brassica napus L.) and Potato (Solanum tuberosum L.).","authors":"Lelde Grantina-Ievina, Nils Rostoks","doi":"10.3390/biotech15020030","DOIUrl":"https://doi.org/10.3390/biotech15020030","url":null,"abstract":"Genetically modified (GM) plants have been commercially grown for 30 years, and their acceptance depends on a thorough risk assessment. Environmental Risk Assessment (ERA) evaluates potential impacts of releasing GM plants into the environment, whether through cultivation or import for food, feed, and processing. A key component is assessing potential gene flow to crop wild relatives or non-GM crops. For gene flow to significantly affect the environment, transferred genes must provide a selective advantage. Since most GM plants are engineered for herbicide tolerance, insect resistance, or stacked traits, evaluating such advantages is relatively straightforward. New genomic techniques (NGTs) can generate plants with a wider range of traits, including tolerance to biotic and abiotic stress. Although still considered GM in the EU, their genomic changes can complicate detection, identification, and ERA, especially when such traits may offer advantages under stress conditions. This scoping review focuses on gene flow in two crops: oilseed rape (canola) (Brassica napus L.) and potato (Solanum tuberosum L.). In canola, transgene movement can increase weediness, fitness, herbicide resistance, or genetic diversity in feral or related populations. Gene flow in potato is less studied, with concerns centered on contamination risks in the Andean diversity center. Limited data exist for NGT plants, though many are expected to resemble conventionally bred varieties, suggesting comparable environmental impacts.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13108222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147783437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DFT Calculations on Electronic, Thermochemical and Vibrational Properties of Se₆ Selenium Clusters as 5-Fluorouracil Drug Delivery System. 5-氟尿嘧啶给药体系Se6硒团簇的电子、热化学和振动性质的DFT计算

IF 3.1

BioTech Pub Date : 2026-03-31 DOI: 10.3390/biotech15020029

Levi Isai Solano-González, Raúl Mendoza-Báez, Ricardo Agustín-Serrano, José Isrrael Rodríguez-Mora, Marco A Morales

{"title":"DFT Calculations on Electronic, Thermochemical and Vibrational Properties of Se6 Selenium Clusters as 5-Fluorouracil Drug Delivery System.","authors":"Levi Isai Solano-González, Raúl Mendoza-Báez, Ricardo Agustín-Serrano, José Isrrael Rodríguez-Mora, Marco A Morales","doi":"10.3390/biotech15020029","DOIUrl":"https://doi.org/10.3390/biotech15020029","url":null,"abstract":"In this work, the electronic, thermochemical, and vibrational characterization of the drug delivery system formed by clusters of selenium (Se6 allotrope) and 5-fluorouracil (5-FU) are studied, based on density functional theory (DFT) calculations. Computational calculations were performed using the B3LYP functional and the 6-31G(d,p) base set, considering an aqueous medium through the CPCM solvation model. We propose evaluating two different interaction modes based on experimental observations: Se-H(N) (through the amino groups of 5-FU) and Se-O(C) (through the carbonyl oxygen of 5-FU). All complexes proved to be energetically stable, exhibiting chemisorption as their adsorption process. Analysis of adsorption energy and thermodynamic parameters indicates that both interaction pathways are equally viable, which agrees with previous experimental findings. The theoretical FT-IR spectra of these complexes also coincide with the experimental results. Furthermore, global molecular descriptors show that the stability of the selenium carrier is not affected by post-functionalization, which is desirable for more controlled drug delivery systems.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13108189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147783408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypoxia and DNA-Repair Radiosensitivity Signatures Are Associated with Radiotherapy-Modified Survival in TCGA Breast Cancer, with External Prognostic Validation of the Hypoxia Score in METABRIC. 缺氧和dna修复放射敏感性特征与TCGA乳腺癌放疗改善生存率相关，并在METABRIC中对缺氧评分进行外部预后验证。

IF 3.1

BioTech Pub Date : 2026-03-31 DOI: 10.3390/biotech15020028

Jimmy Carter Osei, Mei-Han Chen, Tim A D Smith

{"title":"Hypoxia and DNA-Repair Radiosensitivity Signatures Are Associated with Radiotherapy-Modified Survival in TCGA Breast Cancer, with External Prognostic Validation of the Hypoxia Score in METABRIC.","authors":"Jimmy Carter Osei, Mei-Han Chen, Tim A D Smith","doi":"10.3390/biotech15020028","DOIUrl":"https://doi.org/10.3390/biotech15020028","url":null,"abstract":"Radiotherapy (RT) is one of the main treatments for breast cancer, but response varies between patients. Tumour hypoxia and intrinsic radiosensitivity are major determinants of response to RT. Using TCGA-BRCA, a 563-gene hypoxia meta-signature was built by combining curated hypoxia gene sets from MSigDB with published hypoxia metagenes (Buffa, Winter, Elvidge, Fardin, and related sets). After Cox screening and penalised regression, a simple three-gene hypoxia score (CP, GPC3, STC1) was derived. In parallel, based on DSB-repair factors highlighted by Mladenov et al. as key regulators of intrinsic radiosensitivity, a four-gene radiosensitivity (RS) signature (ATR, RPA2, BLM, MRE11A) was trained using only RT-treated patients. In TCGA, both signatures were prognostic and showed significant interaction with RT status in Cox models. The hypoxia score was strongly associated with worse outcomes in RT-untreated patients, but this effect was much weaker in RT-treated patients (Hypoxia × RT HR = 0.009, p = 0.044). The RS score showed a similarly strong interaction with RT (RS × RT HR = 0.011, p = 0.003). When we combined both signatures into one interaction model, it gave the best performance (C-index = 0.785), and both interaction terms stayed independently significant. The hypoxia score was then validated externally in METABRIC (N = 1979; 1143 events), where it remained associated with overall survival, although more weakly than in TCGA (HR = 1.34, 95% CI: 1.10-1.63; p = 0.0042). Overall, these results suggest that hypoxia and DSB-repair capacity capture two complementary sides of radiosensitivity and RT-modified survival patterns, and they support further prospective testing and validation in independent datasets with strong RT annotation.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13108141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147783397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioremediation of Lubricant Oil by Environmentally Adapted Pseudomonas aeruginosa, Pseudomonas putida, and Proteus vulgaris in Houston, Texas. 环境适应性铜绿假单胞菌、恶臭假单胞菌和寻常变形杆菌对润滑油的生物修复。

IF 3.1

BioTech Pub Date : 2026-03-26 DOI: 10.3390/biotech15020027

Sadith Mosquera, Jason A Rosenzweig

{"title":"Bioremediation of Lubricant Oil by Environmentally Adapted Pseudomonas aeruginosa, Pseudomonas putida, and Proteus vulgaris in Houston, Texas.","authors":"Sadith Mosquera, Jason A Rosenzweig","doi":"10.3390/biotech15020027","DOIUrl":"https://doi.org/10.3390/biotech15020027","url":null,"abstract":"Lubricating oil (LO) is manufactured in various formulations for different applications. The inappropriate disposal of petroleum hydrocarbons can increase soil contamination, promoting deleterious environmental and human health impacts. More specifically, following prolonged exposure, LO contaminants are known to have carcinogenic and neurotoxic effects in humans. Bioremediation provides an effective and attractive strategy to expedite the clean-up processes of LO contaminants. We isolated and identified environmentally adapted strains of Pseudomonas aeruginosa, Pseudomonas putida, and Proteus vulgaris from Houston watershed bayou soils. Interestingly, all three exhibited increased resistance, vis-a-vis surrogate strains, to various antibiotic challenges (of chloramphenicol, tetracycline, kanamycin, penicillin, streptomycin, etc.) and increased biofilm formation ranging from 1.6 to 6.7-fold. In fact, all three environmental strains were significantly better at producing enhanced biofilm formation in the presence of spent LO rather than clean LO as well as outproducing biofilm made by the surrogate strains. Finally, the environmental isolates P. aeruginosa, P. putida, and P. vulgaris demonstrated an enhanced ability to sequester clean (2-, 2.5- and 1.14-fold) and spent (1.4-, 1.5, and 1.2-fold) LO when compared to their commercially acquired surrogate reference strains. Our three environmentally isolated organisms from Houston watershed soils appeared to be environmentally adapted to tolerate LO exposures. In the presence of LOs, all three environmentally isolated strains exhibited enhanced growth, enhanced biofilm production, and improved bioaccumulation of LOs relative to commercial reference strains. Taken together, environmentally adapted organisms can promote the bioremediation of contaminants threatening our environment and, potentially, human health.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13108208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147783243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotechnological Potential of Yucca decipiens Trel Based on Proximate Composition, Multi-Elemental Analysis, and Nursery Growth Performance. 基于近似成分、多元素分析和苗期生长性能的丝兰生物技术潜力。

IF 3.1

BioTech Pub Date : 2026-03-25 DOI: 10.3390/biotech15020026

Selena Del Rocío Martínez-Betancourt, Jorge Cadena-Iñiguez, Laura Araceli López-Martínez, Janet María León Morales, Ramón Marcos Soto-Hernández, Gerardo Loera-Alvarado, Víctor Manuel Ruiz-Vera, Concepción López-Padilla

{"title":"Biotechnological Potential of Yucca decipiens Trel Based on Proximate Composition, Multi-Elemental Analysis, and Nursery Growth Performance.","authors":"Selena Del Rocío Martínez-Betancourt, Jorge Cadena-Iñiguez, Laura Araceli López-Martínez, Janet María León Morales, Ramón Marcos Soto-Hernández, Gerardo Loera-Alvarado, Víctor Manuel Ruiz-Vera, Concepción López-Padilla","doi":"10.3390/biotech15020026","DOIUrl":"https://doi.org/10.3390/biotech15020026","url":null,"abstract":"Yucca decipiens is a native species from arid and semi-arid regions with emerging nutritional and biotechnological potential. This study evaluated its proximate composition, elemental profile determined by inductively coupled plasma mass spectrometry (ICP-MS), and growth performance under nursery conditions. Proximate analysis revealed a high dietary fiber content in leaves (58.93%) and higher carbohydrate levels in stems (28.83%). Free amino acid content was significantly higher in stems (2.75 g histidine equivalents kg-1) than in leaves (1.76 g kg-1). Multi-elemental profiling (63 elements) showed organ-specific accumulation patterns, with essential macro- and micronutrients predominantly concentrated in leaves, including potassium (28,334 ppm) and calcium (15,345 ppm), while iron was the most abundant trace element in stems (1253 ppm). Principal component analysis (PCA) revealed clear organ-specific mineral partitioning between leaves and stems, indicating differentiated physiological roles and potential selective biomass utilization. Growth assessment conducted over a two-year period demonstrated steady biomass accumulation and good adaptive performance under nursery conditions. Overall, the results highlight the emerging nutritional and agroindustrial relevance of Yucca decipiens for applications in semi-arid environments.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13108216/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147783446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and Biological Activity of Rutin Extracted from Filipendula ulmaria (L.) Maxim. 乌桕芦丁提取物的特性及生物活性研究的格言。

IF 3.1

BioTech Pub Date : 2026-03-23 DOI: 10.3390/biotech15010025

Anna Vesnina, Violeta Le, Svetlana Ivanova, Anna Frolova, Irina Milentyeva, Victor Atuchin, Alexander Prosekov

{"title":"Characterization and Biological Activity of Rutin Extracted from Filipendula ulmaria (L.) Maxim.","authors":"Anna Vesnina, Violeta Le, Svetlana Ivanova, Anna Frolova, Irina Milentyeva, Victor Atuchin, Alexander Prosekov","doi":"10.3390/biotech15010025","DOIUrl":"10.3390/biotech15010025","url":null,"abstract":"In this work, Filipendula ulmaria (L.) Maxim, a perennial herbaceous plant from the Rosaceae family, was considered a novel source of obtaining rutin for pharmaceutical purposes. Rutin was extracted from the plant parts collected in the flowering summer period and dried at 40 ± 3 °C. The process was carried out using the ethanol extraction and fractionation of extracted compounds, and it yields the 95 wt% purity crystalline product. The phase composition of the extracted rutin was verified by the XRD analysis and NMR measurements. It was found that 2.85% of rutin could be extracted from Filipendula ulmaria, which is 1.2 times higher than the results of similar studies. The biological activity of the isolated rutin was tested on rats. It was established in vivo that the extracted rutin normalizes blood glucose levels (glucose and glycosylated hemoglobin), insulin resistance (HOMA-IR index) and reduces the severity of dystrophic changes in the liver caused by high-fat and high-carbohydrate diets. The introduction of rutin corrects lipid profile indicators (triglycerides, cholesterol, cholesterol fractions in lipoproteins and atherogenic indices), cytolysis indicators of hepatocytes, and liver steatosis (ALT, AST/ALT, triglycerides). Thus, the novel source of rutin opens the possibility for a wide use of this flavonoid in the food technology and pharmaceutical industry.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13024509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147533120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automation of Polysaccharide Quantification: A Rapid High-Throughput Assay Enabled by Liquid Handling Technology. 多糖定量自动化：液体处理技术实现的快速高通量分析。

IF 3.1

BioTech Pub Date : 2026-03-20 DOI: 10.3390/biotech15010024

Samuel Nicacio, Winston Umakanth Balasundaram, Aboli Bhingarkar, Daniel Cho, Rashmi Ghayal, Anup Datta, Subhash V Kapre

{"title":"Automation of Polysaccharide Quantification: A Rapid High-Throughput Assay Enabled by Liquid Handling Technology.","authors":"Samuel Nicacio, Winston Umakanth Balasundaram, Aboli Bhingarkar, Daniel Cho, Rashmi Ghayal, Anup Datta, Subhash V Kapre","doi":"10.3390/biotech15010024","DOIUrl":"10.3390/biotech15010024","url":null,"abstract":"Different methods are used today for polysaccharide quantitation, including HPLC and various colorimetric assays. Among these, the anthrone-sulfuric acid assay (anthrone assay) is popular when the sample matrix is suitable, such as in purified polysaccharides and monovalent bulk conjugate components of glycoconjugate vaccines. While relatively safe, quick, and affordable, the anthrone assay requires significant operator time to complete and is not suited to high-throughput processing. Furthermore, the anthrone-sulfuric acid reagent presents a unique challenge to automation efforts due to its corrosive properties. Reported here is an automated anthrone assay via a liquid handling system (LHS). Twenty-three serotypes of pneumococcal (PNU) polysaccharide were quantified with the traditional anthrone assay and subsequently analyzed using the anthrone LHS method. The anthrone LHS method was evaluated for accuracy compared to the manual method and later validated according to ICH Q2 (R2) guidelines. To our knowledge, this is the first fully unattended and corrosion-mitigated anthrone assay validated under ICH Q2 (R2), capable of overnight batch operation. The developed assay can quantify polysaccharides with an accuracy of 81-115%, is precise to a coefficient of variation of <7.0%, and is linear between 30 and 650 µg/mL range (R2 ≥ 0.993). The assay can process eight samples per hour, can be utilized in overnight operation, and completes all pipetting, incubation, and data export steps automatically.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13023583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147533112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-Transfected Plasmids Enhance Transient Expression of Reporter Genes. 共转染质粒增强报告基因的瞬时表达。

IF 3.1

BioTech Pub Date : 2026-03-04 DOI: 10.3390/biotech15010023

Shih-Yen Lo, Chee-Hing Yang, Yu-Ru Chan, Yi-Tzu Chao, Meng-Jiun Lai, Hui-Chun Li

{"title":"Co-Transfected Plasmids Enhance Transient Expression of Reporter Genes.","authors":"Shih-Yen Lo, Chee-Hing Yang, Yu-Ru Chan, Yi-Tzu Chao, Meng-Jiun Lai, Hui-Chun Li","doi":"10.3390/biotech15010023","DOIUrl":"10.3390/biotech15010023","url":null,"abstract":"Transient DNA transfection is routinely used to study gene function and elucidate the regulation of biological pathways, and it is also widely applied in biotechnology for large-scale recombinant protein production. The results of recent studies involving mammalian cells have highlighted that competition for cellular resources during gene expression can bias data interpretation, directly affecting co-transfection experiments. In this study, our results showed that co-transfected plasmids markedly enhance transient-but not stable-expression of various reporter genes across different cell types. The enhancement of transient reporter gene expression by additional plasmid DNA occurs when these DNAs are co-delivered simultaneously and is unlikely to be mediated by cytokine induction. Furthermore, co-transfected plasmids were shown to upregulate transcription, but not translation, of the reporter gene during transient expression. Thus, the observed enhancement may result from competition between co-transfected plasmids and reporter constructs for cellular proteins that interact with transfected DNA, such as histones. Indeed, Pracinostat (SB939), an inhibitor of histone deacetylase, was able to enhance the transient expression of the reporter gene dose-dependently. Overall, this study provides insights that may facilitate improved transient expression of recombinant genes in biotechnological applications.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13023970/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147533182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial Intelligence and Digital Technology in Cardiovascular Imaging: A Narrative Review. 人工智能与数字技术在心血管成像中的应用综述。

IF 3.1

BioTech Pub Date : 2026-03-03 DOI: 10.3390/biotech15010022

Constantinos H Papadopoulos, Dimitris Karelas, Christina Floropoulou, Konstantina Tzavida, Dimitrios Oikonomidis, Athanasios Tasoulis, Evangelos Tatsis, Ioannis Kouloulias, Nikolaos P E Kadoglou

{"title":"Artificial Intelligence and Digital Technology in Cardiovascular Imaging: A Narrative Review.","authors":"Constantinos H Papadopoulos, Dimitris Karelas, Christina Floropoulou, Konstantina Tzavida, Dimitrios Oikonomidis, Athanasios Tasoulis, Evangelos Tatsis, Ioannis Kouloulias, Nikolaos P E Kadoglou","doi":"10.3390/biotech15010022","DOIUrl":"10.3390/biotech15010022","url":null,"abstract":"The rapid expansion of digital technologies and artificial intelligence (AI) has profoundly transformed cardiovascular imaging, enabling more precise, efficient, and reproducible assessment of cardiac structure and function. This narrative review summarizes recent advances in AI-driven methods across echocardiography, cardiac computed tomography, cardiac magnetic resonance, and nuclear imaging, with emphasis on image acquisition, automated quantification, and diagnostic and prognostic interpretation. We reviewed contemporary literature describing machine-learning and deep-learning applications for image reconstruction, segmentation, radiomics, and multimodal data integration. Current evidence demonstrates that AI improves image quality, reduces acquisition and analysis time, and enables automated, highly reproducible measurements of chamber volumes, function, tissue characterization, coronary anatomy, and myocardial perfusion, while facilitating advanced pattern recognition for differential diagnosis and risk stratification. Furthermore, digital platforms support remote acquisition, tele-echocardiography, and AI-assisted training of non-expert operators. Despite these advances, challenges remain regarding external validation, generalizability across vendors and populations, explainability, data governance, and regulatory compliance. In conclusion, AI and digital technologies are reshaping cardiovascular imaging by enhancing accuracy, efficiency, and accessibility, but their safe and effective clinical integration requires robust multicenter validation, transparent reporting, and ethical-legal frameworks that ensure trust, equity, and accountability.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13024323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147533087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0