IF 2.7

BioTech Pub Date : 2025-05-24 DOI: 10.3390/biotech14020039

Cameron Louttit

{"title":"The Origins and Proliferation of Unfounded Comparisons Regarding the Safety of Mifepristone.","authors":"Cameron Louttit","doi":"10.3390/biotech14020039","DOIUrl":"10.3390/biotech14020039","url":null,"abstract":"As part of the substantial public discourse surrounding the distribution and use of mifepristone, which is used with misoprostol to facilitate drug-induced abortions, claims comparing the safety of this regimen to that of common pharmaceuticals have emerged and proliferated. Offered in forums ranging from social media to the Supreme Court, these claims have so gained public acceptance that they are now echoed without scrutiny and, at times, reference. Yet the simplistic slogan that \"mifepristone is safer than Tylenol\", though easily disseminated, defies both an intuitive understanding of how we evaluate drug safety and our norms and regulations for doing so. Indeed, if such an assertion was attributable to the manufacturer, it would precipitate a reprimand by the FDA given the lack of specific, controlled, and head-to-head evidence rightly required for its support. To the extent that these claims persist, however, including among the outputs of medical societies, abortion centers, clinical researchers, and government officials, and to the extent that they aim to inform both individual and public decision-making, it is critical that the evidence offered for their support be thoroughly explored. Such examination reveals these claims to be wholly unfounded, offering deficient and disingenuous representations of safety for any of the drugs compared.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12191252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategy for the Construction of SARS-CoV-2 S and N Recombinant Proteins and Their Immunogenicity Evaluation. SARS-CoV-2 S和N重组蛋白的构建策略及其免疫原性评价

IF 2.7

BioTech Pub Date : 2025-05-23 DOI: 10.3390/biotech14020038

Paulo Henrique Guilherme Borges, Barbara Gregio, Helena Tiemi Suzukawa, Gislaine Silva-Rodrigues, Emanuella de Castro Andreassa, Isabela Madeira de Castro, Guilherme Bartolomeu-Gonçalves, Emerson José Venancio, Phileno Pinge-Filho, Viviane Monteiro Góes, Celso Vataru Nakamura, Eliandro Reis Tavares, Tatiana de Arruda Campos Brasil de Souza, Sueli Fumie Yamada-Ogatta, Lucy Megumi Yamauchi

{"title":"Strategy for the Construction of SARS-CoV-2 S and N Recombinant Proteins and Their Immunogenicity Evaluation.","authors":"Paulo Henrique Guilherme Borges, Barbara Gregio, Helena Tiemi Suzukawa, Gislaine Silva-Rodrigues, Emanuella de Castro Andreassa, Isabela Madeira de Castro, Guilherme Bartolomeu-Gonçalves, Emerson José Venancio, Phileno Pinge-Filho, Viviane Monteiro Góes, Celso Vataru Nakamura, Eliandro Reis Tavares, Tatiana de Arruda Campos Brasil de Souza, Sueli Fumie Yamada-Ogatta, Lucy Megumi Yamauchi","doi":"10.3390/biotech14020038","DOIUrl":"10.3390/biotech14020038","url":null,"abstract":"This study reports the construction, expression, and purification of synthetic SARS-CoV-2 spike (S) and nucleoprotein (N) containing immunodominant epitopes. The pET28aS_epit construct included epitopes 287-317, 402, 507, 524-598, and 601-640, while the pET28aN_epit construct included residues 42-62, 153-172, and 355-401. Commercial sequences of both proteins were used as controls. The four constructs were expressed using the Escherichia coli BL21(DE3) star strain at 37 °C. The results show that the S protein constructs were insoluble, unlike the N protein constructs. Both recombinant proteins induced immune responses in mice and were recognized by antibodies present in sera from COVID-19-positive and/or SARS-CoV-2-vaccinated humans. No significant differences in immune recognition were observed between our constructs and the commercially available proteins. In conclusion, S_epit and N_epit could be promising starting points for the development of new strategies based on immunological reactions for the control of SARS-CoV-2 infections.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12191430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Genetic Diversity and Population Structure of Australian Passion Fruit Germplasm. 澳洲百香果种质资源遗传多样性及群体结构研究。

IF 2.7

BioTech Pub Date : 2025-05-16 DOI: 10.3390/biotech14020037

Xinhang Sun, Peter Bundock, Patrick Mason, Pragya Dhakal Poudel, Rajeev Varshney, Bruce Topp, Mobashwer Alam

{"title":"Exploring Genetic Diversity and Population Structure of Australian Passion Fruit Germplasm.","authors":"Xinhang Sun, Peter Bundock, Patrick Mason, Pragya Dhakal Poudel, Rajeev Varshney, Bruce Topp, Mobashwer Alam","doi":"10.3390/biotech14020037","DOIUrl":"10.3390/biotech14020037","url":null,"abstract":"Evaluating the genetic variability of germplasms is essential for enhancing and developing superior cultivars. However, there is limited information on cultivated germplasm diversity for Australian passion fruit breeding programs. The genetic diversity of Australian passion fruit (Passiflora spp.), including 94 rootstocks and 95 scions, was evaluated to support breeding programs aimed at enhancing productivity, fruit quality, and overall crop resilience. Rootstocks were genotyped using high-density 24k Diversity Arrays Technology (DArT)-based single-nucleotide polymorphism (SNP) markers, while genetic characterization of scions was conducted using eight simple sequence repeat (SSR) markers. The resulting genetic relationships revealed significant variation within rootstock populations. Bayesian cluster analysis in STRUCTURE showed that the rootstock population was divided into six distinct genetic groups, whereas only two subpopulations were identified among the scion accessions. SNP-based genotyping further highlighted the allelic diversity of Australian rootstocks, suggesting a rich reservoir of genetic traits for rootstock improvement. These findings underscore the importance of preserving and utilizing genetic diversity in Australian passion fruit germplasm to drive the development of superior cultivars with enhanced adaptability and performance under diverse environmental conditions.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioremediation Potential of a Non-Axenic Cyanobacterium Synechococcus sp. for Municipal Wastewater Treatment in the Peruvian Amazon: Growth Kinetics, Ammonium Removal, and Biochemical Characterization Within a Circular Bioeconomy Framework. 在秘鲁亚马逊河流域处理城市污水的非无菌蓝藻共生球菌的生物修复潜力：在循环生物经济框架内的生长动力学、铵离子去除和生化表征。

IF 2.7

BioTech Pub Date : 2025-05-13 DOI: 10.3390/biotech14020036

Remy G Cabezudo, Juan C Castro, Carlos G Castro, Hicler N Rodriguez, Gabriela L García, Paul M Vizcarra, Carmen Ruiz-Huamán, Marianela Cobos

{"title":"Bioremediation Potential of a Non-Axenic Cyanobacterium Synechococcus sp. for Municipal Wastewater Treatment in the Peruvian Amazon: Growth Kinetics, Ammonium Removal, and Biochemical Characterization Within a Circular Bioeconomy Framework.","authors":"Remy G Cabezudo, Juan C Castro, Carlos G Castro, Hicler N Rodriguez, Gabriela L García, Paul M Vizcarra, Carmen Ruiz-Huamán, Marianela Cobos","doi":"10.3390/biotech14020036","DOIUrl":"10.3390/biotech14020036","url":null,"abstract":"Effective wastewater management is critical for mitigating environmental and health impacts in ecologically sensitive regions like the Peruvian Amazon, where rapid urbanization has led to increased discharge of nutrient-rich effluents into freshwater systems. Conventional treatment methods often fail to address nutrient imbalances while generating secondary pollutants. This study aims to evaluate the bioremediation potential of a non-axenic cyanobacterium, Synechococcus sp., isolated from the Amazon Basin, for municipal wastewater treatment within a circular bioeconomy framework. The strain was cultivated in different concentrations of municipal wastewater (25%, 50%, 75%, 100%) from Moronacocha Lake in the Peruvian Amazon to assess growth kinetics, ammonium removal efficiency, and biochemical composition. The cyanobacterium exhibited optimal performance in 25% wastewater, achieving the highest specific growth rate (22.8 × 10-2 μ·day-1) and biomass increase (393.2%), exceeding even the standard BG-11 medium. This treatment also demonstrated exceptional ammonium removal efficiency (95.4%) and enhanced phycocyanin production (33.6 μg/mg, 56% higher than the control). As wastewater concentration increased, both growth parameters and removal efficiency progressively declined. Biochemical analysis revealed that higher wastewater concentrations resulted in decreased protein content and increased lipid accumulation in the biomass. These findings demonstrate the dual potential of Synechococcus sp. for effective wastewater remediation and production of valuable biomass with modifiable biochemical characteristics, offering a sustainable approach for wastewater management in the Peruvian Amazon region.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Potential of Chitinase and Chitosanase from the Strain Bacillus thuringiensis B-387 for the Production of Antifungal Chitosan Oligomers. 苏云金芽孢杆菌B-387几丁质酶和壳聚糖酶生产抗真菌壳聚糖低聚物的比较潜力。

IF 2.7

BioTech Pub Date : 2025-05-08 DOI: 10.3390/biotech14020035

Gleb Aktuganov, Alexander Lobov, Nailya Galimzianova, Elena Gilvanova, Lyudmila Kuzmina, Polina Milman, Alena Ryabova, Alexander Melentiev, Sergey Chetverikov, Sergey Starikov, Sergey Lopatin

{"title":"Comparative Potential of Chitinase and Chitosanase from the Strain Bacillus thuringiensis B-387 for the Production of Antifungal Chitosan Oligomers.","authors":"Gleb Aktuganov, Alexander Lobov, Nailya Galimzianova, Elena Gilvanova, Lyudmila Kuzmina, Polina Milman, Alena Ryabova, Alexander Melentiev, Sergey Chetverikov, Sergey Starikov, Sergey Lopatin","doi":"10.3390/biotech14020035","DOIUrl":"10.3390/biotech14020035","url":null,"abstract":"The depolymerization of chitosan using chitinolytic enzymes is one of the most promising approaches for the production of bioactive soluble chitooligosaccharides (COS) due to its high specificity, environmental safety, mild reaction conditions, and potential for development. However, the comparative efficacy of bacterial chitinases and chitosanases in terms of yield, solubility, and antimicrobial activity of produced COS remains understudied. In this work, chitinase (73 kDa) and chitosanase (40 kDa) from the strain Bacillus thuringiensis B-387 (Bt-387) were purified using various chromatographic techniques and compared by their action on chitosan (DD 85%). The molecular mass and structure of generated COS was determined using TLC, LC-ESI-MS, HP-SEC, and C13-NMR techniques. Chitosanase converted the polymer more rapidly to short COS (GlcN2-GlcN4), than chitinase, and was more specific in its action on mixed bonds between GlcN and GlcNAc. Chitosanase needed a noticeably shorter incubation time and enzyme-substrate ratio than chitinase for production of larger oligomeric molecules (Mw 2.4-66.5 and 15.4-77.7 kDa, respectively) during controlled depolymerization of chitosan. Moreover, chitosanase-generated oligomers demonstrate better solubility and a higher antifungal activity in vitro against the tested plant pathogenic fungi. These features, as well as the high enzyme production and its simplified purification protocol, make chitosanase B-387 more suitable for the production of antifungal chitooligomers than chitinase.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined Effect of Spent Mushroom Substrate and Agro-Industrial Residues on Pleurotus columbinus Production and Intra-Cellular Polysaccharide Synthesis. 食用菌废底物与农工残留物对侧耳生产及胞内多糖合成的联合影响

IF 2.7

BioTech Pub Date : 2025-05-02 DOI: 10.3390/biotech14020034

Marianna Dedousi, Chrysavgi Gardeli, Seraphim Papanikolaou, Panagiota Diamantopoulou

{"title":"Combined Effect of Spent Mushroom Substrate and Agro-Industrial Residues on Pleurotus columbinus Production and Intra-Cellular Polysaccharide Synthesis.","authors":"Marianna Dedousi, Chrysavgi Gardeli, Seraphim Papanikolaou, Panagiota Diamantopoulou","doi":"10.3390/biotech14020034","DOIUrl":"10.3390/biotech14020034","url":null,"abstract":"Spent mushroom substrate (SMS), spent coffee grounds from espresso production (SCG), faba bean harvest residues (FBR), pistachio shells (PS) wheat straw (WS) (control) agro-industrial waste were combined in different ratios, with or without supplements (wheat bran, soybean flour), to create novel substrates for Pleurotus columbinus growth. The impact of the substrates on the mycelial growth rate (Kr), biomass production, laccase, total cellulases and carbohydrate synthesis, along with the C and N consumption by P. columbinus, were examined in fully colonized substrates. The incubation period, earliness and biological efficiency (B.E.) (%) were also determined. Then, the intracellular polysaccharide (ICP) contents of the P. columbinus produced mushrooms were evaluated in the most promising substrates. P. columbinus was grown successfully in a wide range of C/N ratios of substrates and the fastest Kr (7.6 mm/d) was detected on the 70 SMS-30 FBR, without supplements, whereas substrates consisting of SCG enhanced biomass production (700.0-803.7 mg/g d.w.). SMS and PS or SCG led to the shortest incubation and earliness period of P. columbinus. The C content was reduced and the N content was substantially increased in all the colonized substrates. The 70 SMS-30 FBR and 80 SMS considerably enhanced the laccase production (up to 59,933.4 U/g d.w.) and substrates consisting of PS promoted total cellulases activities. Greater amounts of carbohydrates (3.8-17.4 mg/g d.w.) than that in the control were recorded for all the substrates. The combination of SMS and SCG or WS led to the highest B.E. values (59.3-87.1%) and ICP amounts (34.7-45.9%, w/w), regardless of the supplement addition. These findings support the effective utilization of agro-industrial waste in P. columbinus cultivation, producing high-value-added compounds and supporting mushroom growth.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovations in Proteomic Technologies and Artificial Neural Networks: Unlocking Milk Origin Identification. 蛋白质组学技术和人工神经网络的创新：解锁牛奶来源鉴定。

IF 2.7

BioTech Pub Date : 2025-04-28 DOI: 10.3390/biotech14020033

Achilleas Karamoutsios, Emmanouil D Oikonomou, Chrysoula Chrysa Voidarou, Lampros Hatzizisis, Konstantina Fotou, Konstantina Nikolaou, Evangelia Gouva, Evangelia Gkiza, Nikolaos Giannakeas, Ioannis Skoufos, Athina Tzora

{"title":"Innovations in Proteomic Technologies and Artificial Neural Networks: Unlocking Milk Origin Identification.","authors":"Achilleas Karamoutsios, Emmanouil D Oikonomou, Chrysoula Chrysa Voidarou, Lampros Hatzizisis, Konstantina Fotou, Konstantina Nikolaou, Evangelia Gouva, Evangelia Gkiza, Nikolaos Giannakeas, Ioannis Skoufos, Athina Tzora","doi":"10.3390/biotech14020033","DOIUrl":"10.3390/biotech14020033","url":null,"abstract":"Milk's biological origin determination, including its adulteration and authenticity, presents serious limitations, highlighting the need for innovative advanced solutions. The utilisation of proteomic technologies combined with personalised algorithms creates great potential for a more comprehensive approach to analysing milk samples effectively. The current study presents an innovative approach utilising proteomics and neural networks to classify and distinguish bovine, ovine and caprine milk samples by employing advanced machine learning techniques; we developed a precise and reliable model capable of distinguishing the unique mass spectral signatures associated with each species. Our dataset includes a diverse range of mass spectra collected from milk samples after MALDI-TOF MS (Matrix-assisted laser desorption/ionization-time of flight mass spectrometry) analysis, which were used to train, validate, and test the neural network model. The results indicate a high level of accuracy in species identification, underscoring the model's potential applications in dairy product authentication, quality assurance, and food safety. The current research offers a significant contribution to agricultural science, providing a cutting-edge method for species-specific classification through mass spectrometry. The dataset comprises 648, 1554, and 2392 spectra, represented by 16,018, 38,394, and 55,055 eight-dimensional vectors from bovine, caprine, and ovine milk, respectively.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of Multifunctional Hydrolysates from the Lupinus mutabilis Protein Using a Micrococcus sp. PC7 Protease. 利用微球菌PC7蛋白酶从变异狼疮蛋白生产多功能水解物。

IF 2.7

BioTech Pub Date : 2025-04-27 DOI: 10.3390/biotech14020032

Keyla Sofía Llontop-Bernabé, Arturo Intiquilla, Carlos Ramirez-Veliz, Marco Santos, Karim Jiménez-Aliaga, Amparo Iris Zavaleta, Samuel Paterson, Blanca Hernández-Ledesma

{"title":"Production of Multifunctional Hydrolysates from the Lupinus mutabilis Protein Using a Micrococcus sp. PC7 Protease.","authors":"Keyla Sofía Llontop-Bernabé, Arturo Intiquilla, Carlos Ramirez-Veliz, Marco Santos, Karim Jiménez-Aliaga, Amparo Iris Zavaleta, Samuel Paterson, Blanca Hernández-Ledesma","doi":"10.3390/biotech14020032","DOIUrl":"10.3390/biotech14020032","url":null,"abstract":"The growing demand for functional foods has driven the search for bioactive compounds derived from plant proteins. Lupinus mutabilis \"Tarwi\", a legume native to the Peruvian Andes, stands out for its high protein content and potential as a source of bioactive peptides (BPs). In this study, the functionality of the proteins contained in the albumin fraction (AF) isolated by tangential ultrafiltration (TFF) was investigated by using the OmicsBox software. The identified proteins were functionally classified into three groups: cellular component (35.57%), molecular function (33.45%), and biological process (30.97%). The isolated AF was hydrolysed with the native protease PC7 (HAP), optimizing the E/S ratio and time parameters. Additionally, sequential hydrolysis of the PC7 protease and alcalase (HAPA) was performed. In vitro multifunctionality assays, HAP and HAPA demonstrated the ability to scavenge radicals (ABTS and ORAC) and inhibit angiotensin-converting enzyme (ACE)-I and dipeptidyl peptidase IV (DPP-IV). The findings of this study highlight the potential of L. mutabilis albumin hydrolysate as a multifunctional ingredient for functional foods aimed at managing chronic conditions associated with oxidative stress, hypertension, and/or metabolic disorders.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vaginal Microbiota Patterns Associated with Yeast Infection in Mexican Women, a Pilot Study. 墨西哥妇女阴道微生物群模式与酵母菌感染相关，一项初步研究。

IF 2.7

BioTech Pub Date : 2025-04-26 DOI: 10.3390/biotech14020031

Janet Pineda-Díaz, Carolina Miranda-Brito, Carmen Josefina Juárez-Castelán, Alberto Piña-Escobedo, Noemí Del Socorro Lázaro-Pérez, Alejandra de la Cruz-Munguía, Daniela Ramírez-Sánchez, Yuliana Gómez-Meraz, Juan Manuel Vélez-Ixta, Jaime García-Mena

{"title":"Vaginal Microbiota Patterns Associated with Yeast Infection in Mexican Women, a Pilot Study.","authors":"Janet Pineda-Díaz, Carolina Miranda-Brito, Carmen Josefina Juárez-Castelán, Alberto Piña-Escobedo, Noemí Del Socorro Lázaro-Pérez, Alejandra de la Cruz-Munguía, Daniela Ramírez-Sánchez, Yuliana Gómez-Meraz, Juan Manuel Vélez-Ixta, Jaime García-Mena","doi":"10.3390/biotech14020031","DOIUrl":"10.3390/biotech14020031","url":null,"abstract":"Recurrent vulvovaginal candidiasis (RVVC) is a common condition that affects women of reproductive age. The etiology of RVVC remains largely unknown, but it is believed to be associated with changes in vaginal microbiota composition. This study investigates the vaginal microbiota in 57 women with RVVC and 38 healthy controls. Bacterial DNA was analyzed using high-throughput 16S rRNA gene sequencing, and Candida and Saccharomyces species were determined by PCR. RVVC cases had a higher prevalence of Nakaseomyses glabratus (former Candida glabrata) compared to controls. Alpha diversity metrics were similar between groups, but beta diversity analysis revealed significant differences in vaginal microbiota composition. The Firmicutes abundance was altered in RVVC cases, with genus Bifidobacterium and phylum Actinobacteriota being more abundant than in the controls. At the genus level, Lactobacillus dominated controls using antibiotics, while Bifidobacterium was higher in cases with no antibiotic intake. Our study provides evidence that Nakaseomyses glabratus (former Candida glabrata) is a significant pathogen in RVVC, while Candida albicans was more prevalent in healthy women. The vaginal microbiota composition differs significantly between the two groups, with distinct patterns of bacterial abundance and changes in Firmicutes abundance.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and Biotechnology of Three New Strains of Basidial Fungi as Promising Sources of Biologically Active Substances. 三种具有生物活性物质的担子真菌新菌株的鉴定与生物技术研究。

IF 2.7

BioTech Pub Date : 2025-04-25 DOI: 10.3390/biotech14020030

Maria Alexandrovna Sysoeva, Ilyuza Shamilevna Prozorova, Elena Vladislavovna Sysoeva, Tatyana Vladimirovna Grigoryeva, Ruzilya Kamilevna Ismagilova

{"title":"Characterization and Biotechnology of Three New Strains of Basidial Fungi as Promising Sources of Biologically Active Substances.","authors":"Maria Alexandrovna Sysoeva, Ilyuza Shamilevna Prozorova, Elena Vladislavovna Sysoeva, Tatyana Vladimirovna Grigoryeva, Ruzilya Kamilevna Ismagilova","doi":"10.3390/biotech14020030","DOIUrl":"10.3390/biotech14020030","url":null,"abstract":"The study of new strains of basidiomycetes as sources of biologically active substances is a promising direction in modern biotechnology. This work aims to isolate new cultures of the fungi Daedaleopsis tricolor, Pycnoporellus fulgens and Trichaptum abietinum from natural fruiting bodies and to improve their growth conditions on solid nutrient media. The identification of fungi was performed based on their morphological features and using the Sanger sequencing method. Cultivation was carried out by placing inoculum in the middle of a Petri dish and at the edge, which provided a more comprehensive definition of the characteristics of colonies and fungus hyphae. New strains were registered in Genbank Overview. The optimal cultivation temperature was 27 °C for all studied strains. The highest radial growth was observed on synthetic medium for D. tricolor (5.26 mm/day) and T. abietinum (7.5 mm/day), and on synthetic medium with lignin for P. fulgens (2.98 mm/day). The biomass amount of D. tricolor KS11 was 133.25 mg at 9 days of cultivation, that of P. fulgens KS12 was 86.73 mg at 16 days, and that of T. abietinum KS10 was 227.33 mg at 6 days. New strains of fungi can be used to obtain biologically active substances for the food, pharmaceutical and cosmetic industries.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"14 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101347/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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