IF 3.6

BioTech Pub Date : 2026-08-19 DOI: 10.3390/biotech15030070

Eui-Hwan Choi

{"title":"Diverse Roles of Cohesin in Chromosome Dynamics and Stem Cells.","authors":"Eui-Hwan Choi","doi":"10.3390/biotech15030070","DOIUrl":"10.3390/biotech15030070","url":null,"abstract":"The cohesin complex is a highly conserved, ring-shaped protein assembly that plays fundamental roles in chromosome biology. Originally identified as the molecular glue that holds sister chromatids together from DNA replication until cell division, cohesin has since been recognized as a pleiotropic regulator of genome organization, gene expression, DNA repair, and cell fate determination. In embryonic stem cells (ESCs), cohesin's functions extend beyond canonical sister chromatid cohesion to include the maintenance of three-dimensional (3D) chromatin architecture through DNA loop extrusion, regulation of pluripotency-associated transcriptional programs, and facilitation of homologous recombination-mediated DNA repair during the prolonged S phase. Recent discoveries have revealed that meiosis-specific cohesin components, particularly the α-kleisin subunit REC8 and its interacting partner STAG3, are expressed and functionally active in mitotic ESC chromosomes, where they contribute to chromosomal organization and sister chromatid cohesion in concert with mitotic RAD21-containing cohesin. Furthermore, the interplay between cohesin and condensin complexes at shared genomic binding sites has emerged as a critical determinant of chromosome topology, with cohesin depletion leading to aberrant condensin accumulation and chromosome hypercompaction. Importantly, perturbations in cohesin function not only impair ESC self-renewal but also direct lineage-specific differentiation, linking cohesin to stem cell fate determination. Germline mutations in cohesin and its regulators underlie a spectrum of developmental disorders termed cohesinopathies, while somatic mutations are frequently observed in various cancers. This review provides a comprehensive overview of the diverse roles of cohesin in chromosome structure, cell cycle regulation, and stem cell biology, with particular emphasis on recent findings in ESCs that illuminate the complex interplay between mitotic and meiotic cohesin complexes.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13511262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148832983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of Agave Leaf Juice for Optimized Kocuria sediminis AS04 Production and Its Delivery via Immobilized Films to Mitigate Saline Stress in Capsicum annuum var. glabriusculum. 龙舌兰叶汁的优化生产及固定化膜输送缓解盐胁迫的研究

IF 3.6

BioTech Pub Date : 2026-08-15 DOI: 10.3390/biotech15030067

Claudia Estefania Cabrera-Muro, Rosa María Camacho-Ruiz, Miguel Angel Lorenzo-Santiago, Jacobo Rodriguez-Campos, Silvia Maribel Contreras-Ramos

{"title":"Valorization of Agave Leaf Juice for Optimized Kocuria sediminis AS04 Production and Its Delivery via Immobilized Films to Mitigate Saline Stress in Capsicum annuum var. glabriusculum.","authors":"Claudia Estefania Cabrera-Muro, Rosa María Camacho-Ruiz, Miguel Angel Lorenzo-Santiago, Jacobo Rodriguez-Campos, Silvia Maribel Contreras-Ramos","doi":"10.3390/biotech15030067","DOIUrl":"10.3390/biotech15030067","url":null,"abstract":"Halotolerant plant growth-promoting bacteria offer a sustainable strategy to improve crop performance under saline conditions. This study evaluated Agave tequilana Weber var. Azul leaf juice was used as an alternative growth medium for Kocuria sediminis AS04, and the bacterium's ability to alleviate salt stress in chiltepin (Capsicum annuum) was evaluated. The chiltepin seedlings were grown in a specialized chamber and exposed to higher salt levels (200, 400, and 600 mM NaCl) for 10 days. During this time, the protective effect of K. sediminis AS04, which was held in a polymer film, was examined. K. sediminis AS04 grew well in a medium containing 25% agave juice and urea, reaching a density of 1.3 × 1010 CFU mL-1. Compared with conventional Tryptic Soy Broth, the medium formulated from agave leaf juice and urea could reduce the cost per kilogram of biomass by approximately 4-fold. Under severe salinity stress, plants inoculated with immobilized K. sediminis at 600 mM NaCl exhibited the highest shoot biomass (0.31 g-1 plant), root length (50.7 mm), and proline accumulation (11.25 µmol g-1 fresh weight), whereas uninoculated plants displayed reduced biomass (0.16 g plant-1) and shorter roots (34.5 mm). At 600 mM NaCl, inoculation increased shoot biomass, root length, and plant survival by approximately 94%, 47%, and 200%, respectively, compared with uninoculated seedlings. This method values agro-industrial waste and enhances chiltepin performance under high-salinity conditions. It promotes sustainable production and helps create affordable inoculants for agricultural biotechnology.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13510538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148833153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Polyamine Oxidase from Kluyveromyces marxianus with Potential for Total Polyamine Determination. 一种新的多胺氧化酶在马氏克鲁维菌中用于总多胺的测定。

IF 3.6

BioTech Pub Date : 2026-08-15 DOI: 10.3390/biotech15030068

Egor P Sergeev, Denis L Atroshenko

{"title":"A Novel Polyamine Oxidase from Kluyveromyces marxianus with Potential for Total Polyamine Determination.","authors":"Egor P Sergeev, Denis L Atroshenko","doi":"10.3390/biotech15030068","DOIUrl":"10.3390/biotech15030068","url":null,"abstract":"Polyamines and their acetylated derivatives are promising biomarkers for noninvasive cancer diagnostics, creating demand for robust enzymatic tools for their detection. In this study, we screened several phylogenetically diverse yeast polyamine oxidases and identified a new enzyme from Kluyveromyces marxianus (KmaPAO) as a promising candidate for analytical development. Among the tested candidates, only enzymes from K. marxianus and Lachancea thermotolerans were obtained in soluble active form, while only KmaPAO could be purified and characterized in detail. KmaPAO was produced in soluble active form in Escherichia coli at approximately 250 ± 40 mg of active enzyme per liter of culture, purified to near homogeneity in a single IMAC step, and showed a melting temperature of 66.6 ± 0.5 °C. The enzyme preferred acetylated polyamines and spermine, while showing lower activity toward spermidine. Kinetic analysis revealed sub-micromolar or low-micromolar Michaelis constants for several substrates and the highest catalytic efficiency toward spermine, 2.2 × 107 M-1 s-1. Due to its favorable expression level, stability, and substrate profile, KmaPAO represents a promising basis for the development of enzymatic assays for total polyamine determination.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13510455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148832941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risk-Aware Computational Prioritization and Validation Route Design for Medicine-Food Homology Plant Compounds in a Parkinson's Disease Context. 帕金森病背景下药食同源植物化合物的风险意识计算优先级和验证路线设计。

IF 3.6

BioTech Pub Date : 2026-08-10 DOI: 10.3390/biotech15030066

Jinhao Zou, Siyi Wang, Jingjiao Yong, Liangyu Yan, Hong Hui, Ye Sun

{"title":"Risk-Aware Computational Prioritization and Validation Route Design for Medicine-Food Homology Plant Compounds in a Parkinson's Disease Context.","authors":"Jinhao Zou, Siyi Wang, Jingjiao Yong, Liangyu Yan, Hong Hui, Ye Sun","doi":"10.3390/biotech15030066","DOIUrl":"10.3390/biotech15030066","url":null,"abstract":"Network pharmacology studies of medicine-food homology plants have identified broad injury response pathways and hubs that cannot support compound-level or Parkinson's disease (PD)-specific claims. We developed a traceable, non-weighted framework that separates regulatory provenance, PD-context evidence, structural support, and safety/developability liabilities. A ten-plant feasibility panel was locked before overlap with a 1631-gene PD union, yielding 382 plant-associated targets and 190 strict intersections. Leave-one-plant-out analysis retained 173-190 targets, whereas disease source and threshold stress tests showed curation dependence. Whole-blood classifiers showed modest five-fold discrimination (area under the curve, 0.606-0.682) and were excluded from candidate decisions. Redocking-validated AutoDock Vina and protein-ligand interaction fingerprints retained baicalein-MMP9, baicalein-AKT1, and baicalein-BCL2 as caution-tagged follow-up pairs. Quercetin-MMP9 was retained as a liability-tagged comparator, while KCNH2 relations were safety-only. Because no biological validation is presented, these pairs remain hypotheses for prospective MPP+-treated SH-SY5Y testing with orthogonal injury, dopaminergic phenotypes, target dependency, material confirmation and safety controls. Baicalein remains source-pending for the material chain.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13511222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148833184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Orange Peel-Derived Carbon Sources on Nannochloropsis salina Mixotrophic Cultivation. 橘皮碳源对纳米盐绿藻混养栽培的影响。

IF 3.6

BioTech Pub Date : 2026-08-07 DOI: 10.3390/biotech15030065

Carlo Esposito, Giancarlo Aldini, Yanan Yin, Stefano Gandolfi, Gianluca Ottolina, Francesco Secundo

{"title":"Effects of Orange Peel-Derived Carbon Sources on Nannochloropsis salina Mixotrophic Cultivation.","authors":"Carlo Esposito, Giancarlo Aldini, Yanan Yin, Stefano Gandolfi, Gianluca Ottolina, Francesco Secundo","doi":"10.3390/biotech15030065","DOIUrl":"10.3390/biotech15030065","url":null,"abstract":"Microalgae are recognized as sustainable biofactories for metabolites relevant to nutraceutical, pharmaceutical, and marine drug applications. Nannochloropsis salina is notable for its production of bioactive lipids, including the pharmaceutically relevant omega-3 eicosapentaenoic acid (EPA). Here, we evaluated orange peel extract (OPE), a citrus by-product, as a substrate for mixotrophic cultivation of N. salina within a bioeconomy framework. OPE supplementation triggered dose-dependent physiological responses. Among the tested OPE concentrations, 5% supplementation resulted in the highest total fatty acid content, increasing fatty acids from 24.4% (control) to 30.6% and EPA from 6.4% to 9.8%, whereas 10% OPE maintained biomass pigmentation. Higher OPE levels enhanced antioxidant potential, raising total antioxidant capacity from 4.6 (control) to 6.7 mg/g vitamin C equivalents (with 10% OPE), while the highest concentrations induced metabolic stress, reducing biomass and altering lipid composition. Fourier transform infrared spectroscopy analyses revealed biochemical adjustments consistent with metabolic reorganization, including increased intensities of the amide I and II bands associated with protein-rich structures. Overall, OPE emerges as a cost-effective supplement capable of modulating the biochemical quality of N. salina while valorizing agro-industrial residues. The increases in EPA and antioxidant capacity support the potential of OPE-supplemented cultures as a platform for the production of marine-derived bioactive compounds.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13511695/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148832980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Night-Time Biomass and Compositional Dynamics in Chlorella vulgaris: Optimisation of Harvesting Time. 小球藻夜间生物量和组成动态：收获时间的优化。

IF 3.6

BioTech Pub Date : 2026-08-06 DOI: 10.3390/biotech15030064

Sofia Pires, Susana Casal, Tânia G Tavares, José C M Pires, Joana Oliveira

{"title":"Night-Time Biomass and Compositional Dynamics in Chlorella vulgaris: Optimisation of Harvesting Time.","authors":"Sofia Pires, Susana Casal, Tânia G Tavares, José C M Pires, Joana Oliveira","doi":"10.3390/biotech15030064","DOIUrl":"10.3390/biotech15030064","url":null,"abstract":"Global population growth has emphasised the need to have sustainable and alternative sources of nutrients. In this context, microalgae have emerged as a potential solution due to their rich biochemical composition, including high-quality proteins, carbohydrates, lipids, and pigments. This study investigates the variation in microalgal growth and biochemical composition over a light:dark cycle, with a focus on the night period. Batch experiments were performed with eight Chlorella vulgaris cultures over a 7-day period. On the seventh day, biomass samples were collected at four time points in four-hour intervals and stored for subsequent biochemical analyses. During the eight-hour dark period, biomass, carbohydrate, and total chlorophyll concentrations decreased by 9%, 12.5%, and 14.4%, respectively. After four hours of light exposure, these parameters increased significantly by 6%, 15.4%, and 12.7%, respectively. Total protein, carotenoids, and fatty acid contents remained relatively stable throughout the evaluated cycle, although variations were observed in the carotenoid profile. During the dark phase, zeaxanthin decreased by 38.0%, whereas violaxanthin increased by 27.2%, suggesting complementary pigment interconversion consistent with xanthophyll cycle activity. Overall, these results highlight the importance of optimising harvesting time to enhance the production of target compounds in a sustainable production of microalgal biomass.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13510351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148833132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Correlation Across Subpopulations of Single Cells in Subtypes of Acute Myeloid Leukemia. 急性髓系白血病亚型中单细胞亚群的差异相关性

IF 3.6

BioTech Pub Date : 2026-08-05 DOI: 10.3390/biotech15030063

Reginald L McGee, Jake Reed, Gregory K Behbehani, Kevin R Coombes

{"title":"Differential Correlation Across Subpopulations of Single Cells in Subtypes of Acute Myeloid Leukemia.","authors":"Reginald L McGee, Jake Reed, Gregory K Behbehani, Kevin R Coombes","doi":"10.3390/biotech15030063","DOIUrl":"10.3390/biotech15030063","url":null,"abstract":"Mass cytometers can record 40-50 parameters per single cell for millions of cells in a sample. Many methods have been developed to cluster phenotypically similar cells within cytometry data, but there are fewer methods to visualize activity and interactions of pairs of proteins across these populations. We have developed a workflow for analyzing correlations associated with predefined populations. By clustering blood samples from acute myeloid leukemia (AML) patients and normal controls using an established algorithm, we obtained a minimum spanning tree of clusters of single cells. Using surface marker expression, we identified clusters on the tree that belonged to phenotypes of interest. Next, we computed correlations between pairs of proteins in each cluster. We developed a novel, coherent, probability-based statistic to test differences between vectors of correlation coefficients. By comparing all combinations of the normal controls under the statistic, we created an empirical distribution that could provide a conservative threshold of differential correlation. Using this empirically derived distribution to define significance, we compared pooled samples from AML subtypes and normal controls to detect differential correlations. Given the structure present within this cytometry dataset, we found it natural to consider correlations in this manner versus aggregating all data and computing a single correlation. Our approach has the advantage that we can localize the statistical measure to determine contributions from particular phenotypic populations. Differentially correlated pairs of proteins can be further explored as possible testable hypotheses by considering a population's distribution of correlation coefficients or biaxially plotting protein expressions within individual cells in a given population.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13510416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148832949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eisenia andrei and Tenebrio molitor Divergently Restructure the Bacteriome and Mycobiome of Sewage Sludge with Contrasting Biosafety Consequences. 赤色Eisenia andrei和黄粉拟虫（Tenebrio molitor）对污水污泥细菌和真菌群的分化重组及其生物安全后果的对比。

IF 3.6

BioTech Pub Date : 2026-08-03 DOI: 10.3390/biotech15030062

Eduardo Mancilla, Marcos Pérez-Losada, Manuel Aira, Jorge Domínguez

{"title":"Eisenia andrei and Tenebrio molitor Divergently Restructure the Bacteriome and Mycobiome of Sewage Sludge with Contrasting Biosafety Consequences.","authors":"Eduardo Mancilla, Marcos Pérez-Losada, Manuel Aira, Jorge Domínguez","doi":"10.3390/biotech15030062","DOIUrl":"10.3390/biotech15030062","url":null,"abstract":"The use of invertebrates for sewage sludge bioconversion offers a sustainable strategy for waste valorization, yet species-specific effects on microbial communities and biosafety remain unclear. Here, we compared the impacts of the earthworm Eisenia andrei (Ea) and the mealworm Tenebrio molitor (Tm) on the bacteriomes and mycobiomes of sewage sludge (ss) using 16S rRNA and ITS amplicon sequencing. Gut passage in both Ea and Tm markedly altered bacterial and fungal composition relative to ss, but produced distinct community profiles with differential shifts across multiple taxa. Both invertebrates reduced bacterial richness by ~40%, while fungal responses diverged: Ea largely preserved mycobiome richness despite reduced evenness, whereas Tm caused a near-complete collapse (~83% Amplicon Sequence Variant loss). Beta diversity analyses revealed clear, non-overlapping separation among ss, Ea, and Tm for both microbial domains. Tm frass showed strong enrichment of clinically relevant bacterial pathogens, while Ea casts exhibited no such enrichment. For fungi, Ea reshaped pathogen composition, whereas Tm largely eliminated fungal pathogens through broad community collapse. Both treatments reduced predicted antibiotic resistance gene abundance, but functional profiles differed, with Ea showing greater functional stability. These findings demonstrate that microbiome restructuring during bioconversion is species-dependent, with contrasting ecological and biosafety implications for downstream environmental use.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13510514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148832906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of an ExPEC-like Escherichia coli ST536 Isolated from Depurated Retail Mussels: Genomic Insights into Food Safety and One Health Surveillance. 从纯化的零售贻贝中分离出一株类似于大肠杆菌的ST536：对食品安全和健康监测的基因组学见解。

IF 3.6

BioTech Pub Date : 2026-07-31 DOI: 10.3390/biotech15030061

Ricardo J Figueiredo, Guilherme Moreira, Ana Machado, Eliane Silva, Adriano A Bordalo, João R Mesquita

{"title":"Detection of an ExPEC-like Escherichia coli ST536 Isolated from Depurated Retail Mussels: Genomic Insights into Food Safety and One Health Surveillance.","authors":"Ricardo J Figueiredo, Guilherme Moreira, Ana Machado, Eliane Silva, Adriano A Bordalo, João R Mesquita","doi":"10.3390/biotech15030061","DOIUrl":"10.3390/biotech15030061","url":null,"abstract":"Filter-feeding bivalves can accumulate fecal bacteria and antimicrobial resistance determinants, yet routine regulatory monitoring relies mainly on quantitative Escherichia coli criteria. This study provides a whole-genome characterization of an ExPEC-like E. coli ST536 (O21) isolate recovered from commercially depurated retail mussels originating from a Class B harvesting area in Portugal. The isolate was phenotypically identified by MicroScan WalkAway Plus and subjected to antimicrobial susceptibility testing and Oxford Nanopore long-read sequencing, followed by genome assembly, annotation, antimicrobial resistance and virulence screening, plasmid and prophage detection, and phylogenomic analysis. Phenotypically, only resistance to tobramycin was detected using the MicroScan WalkAway system. Genomic analysis revealed a complex mobile element-rich architecture, including multiple resistance-associated determinants, 116 virulence-associated loci, prophage regions, and a mobilizable IncY plasmid carrying CTX-like class A β-lactamase-associated hits detected by ABRicate. Despite its ST536 assignment and ExPEC-associated traits, phylogenomic analysis placed the isolate within a phylogroup A/commensal-like background rather than among classical B2 ExPEC lineages, suggesting acquisition of pathoadaptive modules by an atypical environmental lineage. These findings highlight the potential utility of WGS as a complementary tool and suggest that depurated bivalves may serve as sentinels, warranting further investigation.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13510357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148832960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Germination-Induced Variations in Proximate Composition, Phytochemical Profile and Antioxidant Activities of White Sesame Seeds: A Green Approach for Nutritional Modification. 发芽诱导的白芝麻近似成分、植物化学特征和抗氧化活性的变化：一种绿色的营养修饰方法。

IF 3.6

BioTech Pub Date : 2026-07-29 DOI: 10.3390/biotech15030060

Oneeza Anwar, Iahtisham-Ul-Haq, Ramiz Arif, Waqas Ahmed, Hafiz Ansar Rasul Suleria

{"title":"Germination-Induced Variations in Proximate Composition, Phytochemical Profile and Antioxidant Activities of White Sesame Seeds: A Green Approach for Nutritional Modification.","authors":"Oneeza Anwar, Iahtisham-Ul-Haq, Ramiz Arif, Waqas Ahmed, Hafiz Ansar Rasul Suleria","doi":"10.3390/biotech15030060","DOIUrl":"10.3390/biotech15030060","url":null,"abstract":"Germination is a cost-effective bioprocessing technique for enhancing seed properties such as sesame seeds, an underutilized seed crop. This research evaluates modifications in physicochemical and antioxidant activity of white sesame seeds (Sesamum indicum L.) influenced by germination. In this context, non-germinated seeds and germinated seeds at 24, 48, 72, 96, and 120 h were evaluated separately for their phytochemical profile, including TFC (aluminum chloride colorimetric method), TPC (Folin-Ciocalteu method) and antioxidant properties (DPPH and ABTS radical scavenging assays). Significant variations in the proximate composition, phytochemical densities, and antioxidant activities of white sesame seeds were observed as influenced by germination. The statistical analysis showed an increase in flavonoid content when germination was extended to 48 h, but a decline when extended beyond 48 h, whereas higher polyphenol quantities were observed in sesame seeds germinated for 96 and 120 h. The DPPH activity of germinated (24 h) sesame seed extracts was highest. In contrast, ABTS activity was higher in non-germinated seeds than in seeds germinated for 96 h, and it began to decline at 120 h. In general, a negative correlation between antioxidant activity and extended germination time (120 h) has been observed. For the incorporation of sesame seeds into functional food products, 48 h of germination appeared to have potential suitability for a favorable antioxidant and phytochemical profile.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13510433/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148833110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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