BioTech最新文献

{"title":"High-Resolution Melting Analysis Potential for <i>Saccharomyces cerevisiae</i> var. <i>boulardii</i> Authentication in Probiotic-Enriched Food Matrices.","authors":"Monika Borkowska, Michał Kułakowski, Kamila Myszka","doi":"10.3390/biotech13040048","DOIUrl":"10.3390/biotech13040048","url":null,"abstract":"<p><p>To date, the only probiotic yeast with evidence of health-promoting effects is <i>Saccharomyces cerevisiae</i> var. <i>boulardii</i>. The expanded market including dietary supplements and functional foods supplemented with <i>Saccharomyces cerevisiae</i> var. <i>boulardii</i> creates an environment conductive to food adulterations, necessitating rapid testing to verify product probiotic status. Herein, qPCR-HRM analysis was tested for probiotic yeast identification. The effectiveness of the primer pairs' set was examined, designed to amplify heterogeneous regions in (a) rDNA sequences previously designed to identify food-derived yeast and (b) genes associated with physiological and genotypic divergence of <i>Saccharomyces cerevisiae</i> var. <i>boulardii.</i> Preliminary tests of amplicons' differentiation power enabled the selection of interspecies sequences for <i>18SrRNA</i> and ITS and genus-specific sequences <i>HO</i>, <i>RPB2</i>, <i>HXT9</i> and <i>MAL11.</i> The multi-fragment qPCR-HRM analysis was sufficient for culture-dependent <i>Saccharomyces cerevisiae</i> var. <i>boulardii</i> identification and proved effective in the authentication of dietary supplements' probiotic composition. The identification of <i>S. cerevisiae</i> var. <i>boulardii</i> in complex microbial mixtures of kefir succeeded with more specific intragenus sequences <i>HO</i> and <i>RPB2.</i> The predominance of <i>S. cerevisiae</i> var. <i>boulardii</i> in the tested matrices, quantitatively corresponded to the probiotic-enriched food, was crucial for identification with qPCR-HRM analysis. Considering the reported assumptions, qPCR-HRM analysis is an appropriate tool for verifying probiotic-enriched food.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and Characterization of Sialic Acid-Binding Variable Lymphocyte Receptors from Hagfish.","authors":"Mark Rickard N Angelia, Abigail Joy D Rodelas-Angelia, Cheolung Yang, Sojeong Park, Seung Pyo Jeong, Hyeok Jang, Dennis Berbulla Bela-Ong, Hobin Jang, Kim D Thompson, Taesung Jung","doi":"10.3390/biotech13040046","DOIUrl":"10.3390/biotech13040046","url":null,"abstract":"<p><p>Sialic acid is a diverse group of monosaccharides often found on the termini of <i>N</i>- and <i>O</i>-linked glycans as well as being components of glycoconjugates. Hypersialylation has been associated with the progression of chronic inflammation-mediated diseases such as cardiovascular disease and cancer. Given its role in infection and disease-related processes, sialic acid is a promising target for therapeutic approaches that utilize carbohydrate-binding molecules. In this study, we screened for sialic acid-recognizing variable lymphocyte receptors (VLRBs) or ccombodies from inshore hagfish (<i>Eptatretus burgeri</i>) using a synthetic Neu5Ac-glycoconjugate as an antigen in immunoassay. Resulting ccombodies, 2D8, 5G11, 4A1, and 5F8 were further characterized in terms of their binding activity and specificity. A competitive ELISA using free haptens showed strong inhibition using either <i>N</i>-acetylneuraminic acid (Neu5Ac) and <i>N</i>-glycolylneuraminic acid (Neu5Gc). The half-maximal inhibitory concentrations (IC₅₀) for Neu5Ac ranged from 7.02 to 17.06 mM, with candidates 4A1 and 5G11 requiring the least and highest amounts, respectively. IC₅₀ values for Neu5Gc ranged from 8.12 to 13.91 mM, for 4A1 and 5G11, respectively. Candidate ccombodies also detected naturally occurring sialic acid from known sialoglycoproteins using a dot blot assay. Neu5Gc-5G11 and Neu5Ac-2D8 yielded the strongest and weakest docking interactions with affinity values of -5.9 kcal/mol and -4.9 kcal/mol, respectively. Hydrogen bonding and hydrophobic interactions were predicted to be the predominant noncovalent forces observed between the ccombodies and sialic acid. This study demonstrates that glycan-binding VLRBs from hagfish hold promise in augmenting the glycobiologists' toolkit in investigating the roles of glycans in human and animal health and disease.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial Insemination as a Possible Convenient Tool to Acquire Genome-Edited Mice via In Vivo Fertilization with Engineered Sperm.","authors":"Masahiro Sato, Emi Inada, Issei Saitoh, Kazunori Morohoshi, Shingo Nakamura","doi":"10.3390/biotech13040045","DOIUrl":"10.3390/biotech13040045","url":null,"abstract":"<p><p>Advances in genome editing technology have made it possible to create genome-edited (GE) animals, which are useful for identifying isolated genes and producing models of human diseases within a short period of time. The production of GE animals mainly relies on the gene manipulation of pre-implantation embryos, such as fertilized eggs and two-cell embryos, which can usually be achieved by the microinjection of nucleic acids, electroporation in the presence of nucleic acids, or infection with viral vectors, such as adeno-associated viruses. In contrast, GE animals can theoretically be generated by fertilizing ovulated oocytes with GE sperm. However, there are only a few reports showing the successful production of GE animals using GE sperm. Artificial insemination (AI) is an assisted reproduction technology based on the introduction of isolated sperm into the female reproductive tract, such as the uterine horn or oviductal lumen, for the in vivo fertilization of ovulated oocytes. This approach is simpler than the in vitro fertilization-based production of offspring, as the latter always requires an egg transfer to recipient females, which is labor-intensive and time-consuming. In this review, we summarize the various methods for AI reported so far, the history of sperm-mediated gene transfer, a technology to produce genetically engineered animals through in vivo fertilization with sperm carrying exogenous DNA, and finally describe the possibility of AI-mediated creation of GE animals using GE sperm.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11587059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultraviolet, Did the Cell See It from the Side or the Bottom? Assessment and Modeling of UV Effects on Cultured Cells Using the CL-1000 UV-Crosslinker.","authors":"Takahiro Oyama, Kai Yanagihara, Anna Arai, Takanori Kamiya, Midori Oyama, Takashi Tanikawa, Takehiko Abe, Tomomi Hatanaka","doi":"10.3390/biotech13040044","DOIUrl":"10.3390/biotech13040044","url":null,"abstract":"<p><p>Numerous natural extracts and compounds have been evaluated for their ability to mitigate the adverse effects of ultraviolet (UV) overexposure. However, variability in the UV doses that trigger biological responses across studies likely arises from inconsistencies in UV exposure standardization. We hypothesize that these discrepancies are due to variations in culture plates and dishes. The UV dose (D) required to reduce cell viability by 50% differed by a factor of ten between 3.5 cm dishes and 96-well plates. Similarly, the EC₅₀ dose for IL-6 release (<i>D</i>_1/2) varied, potentially correlating with the surface area (S). UV exposure to wells with increasing height in 3.5 cm dishes resulted in a decrease in IL-6 release, suggesting that the greater the well height, the more it may influence UV exposure through reflection or shielding effects, thereby contributing to the physiological effects on the cells. To compare these differences among plates, we defined the height-to-diameter ratio (r). Analysis revealed a linear correlation between <i>D</i>_1/2 and S in a log-log plot, and between <i>D</i>_1/2 and r in a semi-log plot. From this, we defined two empirical indices <i>σ</i> and ρ for UV dose adjustment. A deductive model was also developed to derive a D' value that adjusts UV doses without requiring training. As with σ and ρ, the UV dose D was effectively adjusted using D' as well. These attempts suggest that D' offers a foundational framework for evaluating UVB effects on cultured cells.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11587097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial Protein and Metabolite Profiles of <i>Klebsiella oxytoca M5A1</i> in a Bubble Column Bioreactor.","authors":"Tawakalt Ayodele, Musiliu Liadi, Abodunrin Tirmidhi Tijani, Kudirat Alarape, Christiana Bitrus, Clairmont L Clementson, Ademola Hammed","doi":"10.3390/biotech13040043","DOIUrl":"https://doi.org/10.3390/biotech13040043","url":null,"abstract":"<p><p>The production of microbial proteins (MPs) has emerged as a critical focus in biotechnology, driven by the need for sustainable and scalable alternatives to traditional protein sources. This study investigates the efficacy of two experimental setups in producing MPs using the nitrogen-fixing bacterium <i>Klebsiella oxytoca M5A1</i>. <i>K. oxytoca M5A1</i>, known for its facultative anaerobic growth and capability to fix atmospheric nitrogen, offers a promising avenue for environmentally friendly protein production. This research compares the performance of a simple bubble column (BC) bioreactor, which promotes efficient mixing and cross-membrane gas transfer, with static fermentation, a traditional method lacking agitation and aeration. The study involved the parallel cultivation of <i>K. oxytoca M5A1</i> in both systems, with key parameters such as microbial growth, glucose utilization, protein concentration, and metabolite profiles monitored over a 48 h period. The results indicate that the BC bioreactor consistently outperformed static fermentation regarding the growth rate, protein yield, and glucose utilization efficiency. The BC exhibited a significant increase in protein production, reaching 299.90 µg/mL at 48 h, compared to 219.44 µg/mL in static fermentation. The organic acid profile reveals both synthesis and utilization regimes of varying patterns. These findings highlight the advantages of the BC bioreactor for MP production, particularly its ability to maintain aerobic conditions that support higher growth and yield.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11503442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Composition, Antioxidant, Anti-<i>Helicobacter pylori</i>, and Enzyme Inhibitory Evaluations of <i>Cleistocalyx operculatus</i> Flower Bud and Leaf Fractions.","authors":"Doan Thien Thanh, Mai Thanh Tan, Nguyen Thi My Thu, Pham Nhat Phuong Trinh, Pham Thi Hoai Thuong, Pham Thi Giang Tuyet, Luong Thi My Ngan, Tran Trung Hieu","doi":"10.3390/biotech13040042","DOIUrl":"https://doi.org/10.3390/biotech13040042","url":null,"abstract":"<p><p>Six solvent fractions isolated from flower bud and leaf ethanolic extracts of <i>Cleistocalyx operculatus</i> were analyzed for their phytochemical contents, including phenolics, flavonoids, saponins, tannins, and alkaloids. Antioxidant activities were measured using the ABTS, DPPH, and FRAP assays. The results showed that the flower bud aqueous fraction (BAF) and the leaf aqueous fraction (LAF) rich in phenolic content (768.18 and 490.74 mg GAE/g dry extract, respectively) exhibited significantly higher antioxidant activities than the other fractions. The flower bud hexane fraction (BHF) had remarkably high flavonoid and saponin contents (134.77 mg QE/g and 153.33 mg OA/g dry extract, respectively), followed by that of the leaf hexane fraction (LHF) (76.54 mg QE/g and 88.25 mg OA/g dry extract, respectively). The BHF and LHF were found to have extremely high antibacterial activity against two <i>H. pylori</i> strains, ATCC 51932 and 43504 (MICs of 125 µg/mL). Interestingly, DMC (2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone) isolated from the BHF displayed greater antibacterial activity against the bacterial strains (MICs of 25-50 µg/mL) than those of the fractions. In addition, DMC presented potent inhibitory effects on <i>H. pylori</i> urease (IC₅₀ of 3.2 µg/mL) and α-amylase (IC₅₀ of 83.80 µg/mL), but no inhibition against α-glucosidase. It was also demonstrated that DMC showed pronounced inhibitory effects on the urease activity and biofilm formation of <i>H. pylori</i>, and could increase the membrane permeability of the bacterial cells. Scanning electron micrographs depicted that the BHF and DMC had strong effects on the cell shape and significantly induced the distortion and damage of the cell membrane. The fractions and DMC showed no significant toxicity to four tested human cell lines. Efforts to reduce antibiotic use indicate the need for further studies of the flower buds and DMC as potential products to prevent or treat gastric <i>H. pylori</i> infections.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11503338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural Analysis and Substrate Specificity of D-Carbamoylase from <i>Pseudomonas</i>.","authors":"Marina Paronyan, Haykanush Koloyan, Hovsep Aganyants, Artur Hambardzumyan, Tigran Soghomonyan, Sona Avetisyan, Sergey Kocharov, Henry Panosyan, Vehary Sakanyan, Anichka Hovsepyan","doi":"10.3390/biotech13040040","DOIUrl":"https://doi.org/10.3390/biotech13040040","url":null,"abstract":"<p><p>The synthesis of enantiomeric forms of D-amino acids can be achieved by a two-step \"hydantoinase process\" based on the sequential catalysis of substrates by specific enzymes, D-carbamoylase and D-hydantoinase. Here, we describe the structural features of D-carbamoylase from <i>Pseudomonas</i>, the encoded gene of which was chemically synthesized and cloned into <i>Escherichia coli</i>. A significant fraction of the overexpressed recombinant protein forms insoluble inclusion bodies, which are partially converted to a soluble state upon treatment with N-lauroylsarcosine or upon incubation of cells at 28 °C. Purified His-tagged protein exhibits the highest activity towards N-carbamoyl-D-alanine and N-carbamoyl-D-tryptophan. Comprehensive virtual analysis of the interactions of bulky carbamylated amino acids with D-carbamoylase provided valuable information. Molecular docking analysis revealed the location of the substrate binding site in the three-dimensional structure of D-carbamoylase. Molecular dynamics simulations showed that the binding pocket of the enzyme in complex with N-carbamoyl-D-tryptophan was stabilized within 100 nanoseconds. The free energy data showed that Arg176 and Asn173 formed hydrogen bonds between the enzyme and substrates. The studies of D-carbamoylases and the properties of our previously obtained D-hydantoinase suggest the possibility of developing a harmonized biotechnological process for the production of new drugs and peptide hormones.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11503299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss of Cell-Cell Contact Inhibits Cellular Differentiation of α-Catenin Knock Out P19 Embryonal Carcinoma Cells and Their Colonization into the Developing Mouse Embryos.","authors":"Masahiro Sato, Emi Inada, Naoko Kubota, Masayuki Ozawa","doi":"10.3390/biotech13040041","DOIUrl":"https://doi.org/10.3390/biotech13040041","url":null,"abstract":"<p><p>Cadherin-catenin cell-cell adhesion complexes, composed of cadherin, β-catenin or plakoglobin, and α-catenin (α-cat) molecules, are crucial for maintaining cell-cell contact and are commonly referred to as \"adherens junctions (AJs).\" Inactivating this system leads to loss of cell-cell contact and developmental arrest in early embryos. However, it remains unclear whether the loss of cell-cell contact affects the differentiation of embryonic cells. In this study, we explored the use of a murine embryonal carcinoma cell line, P19, as an in vitro model for early embryogenesis. P19 cells easily form embryoid bodies (EBs) and are susceptible to cellular differentiation in response to retinoic acid (RA) and teratoma formation. Using CRISPR/Cas9 technology to disrupt the endogenous <i>α-cat</i> gene in P19 cells, we generated <i>α-cat</i> knockout (KO) cells that exhibited a loss of cell-cell contact. When cultivated on non-coated dishes, these <i>α-cat</i> KO cells formed EBs, but their structures were labile. In the RA-containing medium, the <i>α-cat</i> KO EBs failed to produce differentiated cells on their outer layer and continued to express SSEA-1, an antigen specific to pluripotent cells. Teratoma formation assays revealed an absence of overt differentiated cells in tumors derived from <i>α-cat</i> KO P19 cells. Aggregation assays revealed the inability of the KO cells to colonize into the zona pellucida-denuded 8-cell embryos. These findings suggest that the AJs are essential for promoting the early stages of cellular differentiation and for the colonization of early-developing embryos.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Sugar Extraction Process from Date Waste Using Full Factorial Design Toward Its Use for New Biotechnological Applications.","authors":"Islam Sayah, Mondher Njehi, Nicola Cicero, Vincenzo Nava, Manel Ben M'hadheb, Hatem Majdoub, Sami Achour, Teresa Gervasi","doi":"10.3390/biotech13040039","DOIUrl":"https://doi.org/10.3390/biotech13040039","url":null,"abstract":"<p><p>In Tunisia, the date industry generates a large quantity of waste, raising environmental concerns. However, dates are rich in sugars, which offer a renewable source of nutrients for various applications. In this study, sugar extraction from two low-grade pitted date fruits (Alig and Kentichi) under ultrasound, was optimized using full factorial design. At 40 °C, for20 min, and with a liquid-to-solid ratio of 10 mL/g, the optimum sugar contents were 60.87% and 50.79% for the varieties Alig and Kentichi, respectively. The date extracts were chemically analyzed, revealing low fat and protein contents, but significant polyphenol and mineral contents in both varieties. HPLC-IR analysis revealed more inverted sugars (glucose and fructose) in the Alig variety and more sucrose in the Kentichi variety. FTIR and SEM analysis showed the efficiency of the ultrasonic treatment of the biomass in terms of improving mass transfer diffusion through ultrasonic cavitation. Thus, ultrasound-assisted extraction constitutes an effective method for the recovery of sugar from date waste.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11503435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical, Textural and Antioxidant Properties of Oat-Fermented Beverages with Different Starter Lactic Acid Bacteria and Pectin.","authors":"Dmitrii V Khrundin, Elena V Nikitina","doi":"10.3390/biotech13040038","DOIUrl":"https://doi.org/10.3390/biotech13040038","url":null,"abstract":"<p><p>Currently, starter cultures for fermenting plant-based beverages are not widely available commercially, but producers can use starter cultures for dairy products. Therefore, the aim of this study was to determine the physicochemical, rheological, antioxidant and sensory properties of oat beverages with/without pectin fermented by four different dairy starter cultures. The use of a mono-starter with <i>Lactobacillus bulgaricus</i> or <i>Sreptococcus thermophilus</i> allows for the efficient use of glucose, and more lactic acid is accumulated. The beverage with <i>L. bulgaricus</i> is characterised by high adhesion, syneresis and low cohesiveness, and it has high antioxidant activity and a low sensory profile. Using starter with <i>L. bulgaricus</i>, <i>S. thermophilus</i> and some <i>Lactococcus</i> for fermentation yields a product with high sensory capacity, forming a high-viscosity beverage matrix with low syneresis, high water retention, chewy texture and stickiness. It has been observed that the absence of lactococci and the presence of <i>Lactobacillus casei</i>, <i>L. Rhamnosus</i> and <i>L. paracasei</i> in the starter yields a product with high antioxidant activity, especially in the presence of pectin. The use of pectin significantly improves the viscosity and textural properties of oat yoghurt, enhancing the drink's flavour and giving it body. For many reasons, the use of different commercial starters in the dairy industry results in different viscosities of oat fermented beverages, forming a matrix with different textural, sensory and antioxidant properties.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11503288/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}