BioTech最新文献

{"title":"Establishment of a High-Efficiency In Vitro Regeneration and <i>Agrobacterium</i>-Mediated Genetic Transformation System for <i>Ajania achilleoides</i> (Turcz.) Poljakov.","authors":"Xiaoyue Zheng, Hao Li, Shu Wang, Huiyan Cheng, Huien Zhao","doi":"10.3390/biotech15030059","DOIUrl":"10.3390/biotech15030059","url":null,"abstract":"<p><p><i>Ajania achilleoides</i>, a wild species with strong environmental adaptability, is a valuable germplasm resource for genetic improvement and functional gene studies. However, efficient in vitro regeneration and genetic transformation systems for this species remain undeveloped, limiting functional gene characterization and genetic improvement of this valuable germplasm resource. In this study, leaf explants were used to establish an efficient regeneration system by evaluating different combinations of plant growth regulators and to develop an <i>Agrobacterium</i> tumefaciens-mediated genetic transformation system for <i>A. achilleoides</i>. MS medium supplemented with 2.0 mg/L 6-benzyladenine (6-BA) and 1.0 mg/L α-naphthaleneacetic acid (NAA) achieved the highest adventitious shoot regeneration rate (99%), while 1/2 MS medium containing 0.5 mg/L indole-3-butyric acid (IBA) was optimal for rooting, resulting in a 100% rooting rate. Critical hygromycin concentrations for shoot regeneration and rooting selection were determined to be 25 mg/L and 10 mg/L, respectively. For transformation, the best results were obtained with a 3-day preculture, bacterial suspension at OD₆₀₀ = 0.2, 30 min infection, and 2-day co-cultivation. PCR and sequencing confirmed the successful integration of the target genes into the genome, yielding three positive transgenic lines with a molecular confirmation rate of 30%. This study establishes a stable and efficient regeneration and transformation system for <i>A. achilleoides</i>, providing a platform for functional gene analysis and molecular breeding of wild chrysanthemum species.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13511080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148833060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Omics Approaches in Hantavirus Research: Current Advances, Challenges, and Future Perspectives.","authors":"Soroosh Najafi, Maryam Jojani, Kianoosh Najafi, Giovanni N Roviello","doi":"10.3390/biotech15030058","DOIUrl":"10.3390/biotech15030058","url":null,"abstract":"<p><p>Hantaviruses are zoonotic RNA viruses responsible for two severe human diseases: hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome (HCPS). Worldwide case fatality rates vary from 1% to 40%. Although single-layer genomics, transcriptomics, proteomics, and metabolomics studies have advanced our understanding of hantavirus biology, true multi-omics integration remains scarce, leaving systems-level mechanisms of disease severity and host-pathogen interactions unresolved. High-throughput omics technologies have greatly advanced the study of hantavirus-host interactions. This review summarizes genomic, transcriptomic, proteomic, metabolomic, and AI-enabled approaches in hantavirus research. Genomic studies have clarified viral diversity, evolution, and reassortment, while transcriptomics has identified regulatory networks governing endothelial and innate immune responses. Proteomics has revealed host proteins involved in immune regulation, endothelial dysfunction, and potential therapeutic targeting, whereas metabolomics indicates substantial metabolic reprogramming during infection, although dedicated studies remain limited. AI-based approaches are increasingly applied to outbreak prediction, surveillance, and risk modeling. Drawing on successful multi-omics frameworks developed for other viral infections, we discuss opportunities to improve biomarker discovery, surveillance, therapeutic development, and future precision medicine strategies for hantavirus infections.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13511750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148833196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Psychrophilic Quorum Sensing Genes Enable Unimodal, Adjustable Protein Expression Across the Entire <i>Escherichia coli</i> Population.","authors":"Ekaterina Scheglova, Sabina Nebieva, Kamilla Mekhantseva, Siarhei Bukhalovich, Anna Kudryavtseva, Nikolay Bondarev, Nikolay Ilyinsky, Sergey Bazhenov, Ilya Manukhov","doi":"10.3390/biotech15030057","DOIUrl":"10.3390/biotech15030057","url":null,"abstract":"<p><p>A previous study demonstrated that quorum sensing (QS) genes from the psychrophilic bacterium <i>Aliivibrio logei</i> can be used in <i>Escherichia coli</i> to obtain bacterial cultures with controlled amounts of a target protein. However, the QS system may be induced non-uniformly across the cell population. In this study, we used an expression vector containing <i>luxR/luxI</i> regulatory genes from <i>A. logei</i> carrying <i>sfGFP</i> as a reporter gene. Reporter expression was regulated by the autoinducer 3OxoC6-HSL, activated at 22 °C, and terminated at 37 °C. Flow cytometry was used to assess the GFP fluorescence distribution at the single-cell level. The system provided dose-dependent unimodal expression lacking formation of distinct ON/OFF subpopulations, while the robust coefficient of variation decreased with increasing autoinducer concentration. The autoinducer synthase LuxI enabled autoinduction, but under the tested conditions, no substantial effect on expression homogeneity was detected. Raising the temperature to 37 °C effectively halted expression, allowing intermediate target protein values to be fixed at the single-cell level. Overall, the developed system represents a promising tool for biotechnological applications requiring precise and uniform control of expression.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13398223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148580397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Betalain Biosynthesis and Metabolic Engineering for Sustainable Natural Pigment Production.","authors":"Saravanan Monisha, Marimuthu Kanchana, Aiyar Balasubramanian, Rajendran K Selvakesavan","doi":"10.3390/biotech15030056","DOIUrl":"10.3390/biotech15030056","url":null,"abstract":"<p><p>Betalains are water-soluble pigments containing nitrogen, and they exist naturally in the plants of the order Caryophyllales. They have gained increasing attention in recent years because of their intense colours, antioxidant activity, and safety, thus making them suitable replacements for artificial dyes. The increasing interest in natural pigments has led to intensified research on betalain biosynthesis and optimization of pigment production. Nonetheless, their application in industry faces limitations, such as their low natural occurrence, sensitivity to environmental conditions, and instability during manufacturing and storage. Unlike previous reviews that primarily focused on betalain chemistry, biosynthesis pathways, or biological activity, the present review highlights recent developments in the engineering of the biosynthesis pathways, synthetic biology, elicitation approaches, omics-based pathway identification, and nanobiotechnology for betalain pigments. Special attention is paid to the comparison of plant, plant cell, yeast, and bacterial production systems, as well as recent advancements towards industrial production of betalain pigments and bottlenecks in the commercialization of sustainable betalain bio-factories.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13397832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148580266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AAV Vector Toolkit for the Delivery and Expression of the Artificial microRNA in the Murine Heart.","authors":"Ivan I Galkin, Viktoriia V Skopenkova, Maria Y Shubina, Anna V Polikarpova, Svetlana G Vassilieva, Irina M Savchenko, Olga S Lebedeva, Daria V Goliusova, Margarita Y Sharikova, Vladimir V Gureev, Tatiana N Malorodova, Alexey V Deikin, Tatiana V Egorova, Maryana V Bardina","doi":"10.3390/biotech15030055","DOIUrl":"10.3390/biotech15030055","url":null,"abstract":"<p><strong>Background: </strong>Adeno-associated virus (AAV) vector-mediated gene transfer is an emerging treatment strategy for severe cardiac disorders with genetic etiology. We refined the AAV toolkit to achieve efficient and selective expression of the therapeutic transgene in mouse hearts.</p><p><strong>Methods: </strong>Using vectors with a reporter transgene, we evaluated AAV administration routes, AAV serotype tropism to the myocardium, and cardiospecific promoters.</p><p><strong>Results: </strong>We showed that systemic AAV administration provides potent delivery and uniform transduction of cardiac tissue, outperforming localized injection techniques. The MyoAAV 2A capsid variant enabled an improved heart-to-liver transduction ratio compared to the parental AAV9 serotype. Screening a panel of cardiac and pan-muscular promoters in vitro and in vivo verified the superiority of the cardiac troponin T (cTnT) promoter for robust heart-specific transgene expression. Finally, we demonstrated that the cumulative properties of systemic AAV delivery, the MyoAAV 2A serotype, and the cTnT promoter allowed for efficient cardiac synthesis of the therapeutic transgene-an artificial miRNA designed for the gene suppression strategy of FLNC-related cardiomyopathy.</p><p><strong>Conclusions: </strong>Our findings establish an effective AAV approach for transgene transfer into the mouse heart and promote the development of gene therapy for cardiac disorders.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13398323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148580330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular Matrix-Based and Extracellular Matrix-Bioinspired Scaffolds for Extracellular Vesicle Delivery in Dental Pulp Regeneration: A Narrative Review.","authors":"Nevena Cvetic, Anđelka Zivaljevic, Kristina Krstic, Suzana Zivanovic, Milos Papic, Natalija Arsenijevic, Miona Glisic, Tamara Milunovic, Renata Petrovic, Milica Popovic","doi":"10.3390/biotech15030054","DOIUrl":"10.3390/biotech15030054","url":null,"abstract":"<p><p>Vital pulp therapy aims to preserve pulp vitality by stimulating reparative processes. However, conventional approaches often result in incomplete tissue regeneration. Extracellular vesicles (EVs) have emerged as promising cell-free therapeutic agents because of their ability to regulate angiogenesis, odontogenesis, and immune responses through the transfer of bioactive molecules. Despite their significant regenerative potential, the clinical application of EVs remains limited by rapid clearance, insufficient local retention, and uncontrolled release following administration. To address these challenges, various extracellular matrix (ECM)-based and ECM-bioinspired scaffolds have been developed as delivery platforms. These scaffolds can provide structural support and enable controlled, localized release of EVs. This narrative review critically evaluates the current evidence regarding scaffold systems as EV delivery platforms for dental pulp regeneration, comparing their biological performance, methodological quality, and translational potential. Across the available studies, scaffold-assisted EV delivery consistently enhanced angiogenesis, odontogenic differentiation, mineralization, and immunomodulation; however, the evidence remains preclinical and is characterized by substantial heterogeneity regarding EV source, isolation and characterization methods, scaffold composition, experimental models, and outcome assessment. Current findings support the feasibility of scaffold-assisted EV delivery for regenerative endodontics, but important challenges remain, including standardization of EV production and characterization, scalable manufacturing, regulatory approval, and demonstration of long-term safety and functional pulp-dentin complex regeneration. Further well-designed translational and clinical studies will be essential before routine clinical implementation can be considered.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13398307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148580261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA-seq Co-Expression Analysis Reveals a Midgut-Associated Digestive Gene Module in <i>Helicoverpa armigera</i>.","authors":"Bairon J Matabanchoy Pejendino, Vicente E Mallama Cadena, María C Díaz Rodríguez, Claudia Salazar Gonzalez, Pedro A Velasquez-Vasconez","doi":"10.3390/biotech15030053","DOIUrl":"10.3390/biotech15030053","url":null,"abstract":"<p><p><i>Helicoverpa armigera</i> is one of the most destructive polyphagous pests, yet the transcriptional organization underlying its digestive capacity remains poorly resolved. Here, we compiled 579 publicly available RNA-seq libraries representing 54 independent experiments and quantified transcript abundance across tissues and developmental stages. This complete dataset was used to support broader tissue-level expression profiling. After metadata harmonization and quality filtering, a subset of 130 biologically comparable libraries from five tissue/developmental categories was retained for weighted gene co-expression network analysis. WGCNA identified four biologically informative modules, among which the turquoise module was positively associated with fourth- and fifth-instar larval midgut samples. Independent expression profiling revealed strong midgut-biased expression of several trypsin- and chymotrypsin-like serine proteases, although only a subset of these genes was assigned to the turquoise module. Descriptive functional annotation of this module identified 202 co-expressed loci, including digestive enzymes, nutrient transporters, detoxification-related proteins, epithelial components and putative transcriptional or signaling-associated genes. Phylogenetic analyses and manual inspection of genomic locations further showed that several digestive protease genes occur in local clusters and have closely related counterparts in <i>H. zea</i>, suggesting partial conservation of local genomic organization. Collectively, these results describe a midgut-associated co-expression module containing genes associated with digestive, absorptive and protective functions and provide candidate genes for future functional studies.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13398162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148580380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global Patent Landscape and Technological Trends in Biosafety Level 3 (BSL-3) Laboratories Technologies.","authors":"Milca de J Silva, Roni D Vinhas, Helena S da Hora, Saada L C Fernandez, Hayna Malta-Santos, Hugo Saba, Camila D F Ribeiro, Marilda de S Gonçalves, Bruna A S Machado","doi":"10.3390/biotech15030052","DOIUrl":"10.3390/biotech15030052","url":null,"abstract":"<p><p>Biosafety Level 3 (BSL-3) laboratories are essential for handling high-risk pathogens and strengthening global health security. This study presents a patent landscape analysis of BSL-3-related technologies using the Derwent World Patents Index (DWPI) to identify technological trends, geographic distribution, patent classifications, and temporal evolution. Patent documents associated with laboratory infrastructure, ventilation systems, containment devices, and biosafety procedures were screened and analyzed. A total of 58 patent documents filed between 2009 and 2024 met the inclusion criteria. The results showed that China and the United States are the leading contributors to BSL-3 patent development, reflecting continued investments in biosafety and biosecurity infrastructure. The most frequent International Patent Classification (IPC) categories were C12M (microbiological devices), E04H (specialized construction infrastructure), and F24F (ventilation and air control systems), highlighting the multidisciplinary nature of innovations in laboratory containment and safety. The temporal trends revealed increases in patent activity following major public health emergencies, including SARS, Ebola, and particularly the COVID-19 pandemic. Furthermore, a significant increase in patent expirations is expected by 2029, creating opportunities for technology transfer, open innovation, and broader access to critical biosafety technologies. These findings emphasize the strategic importance of continued investment in BSL-3 technologies, especially in developing countries with growing biosafety demands.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13397928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148580439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stabilizing Nanoemulsions with Blended Biosurfactants: Role of Sophorolipids and Lecithin in Emulsion Performance.","authors":"Yew Seng Leow, Dayang Radiah Awang Biak, Nur Syakina Jamali, Huey Fang Teh, Norhafizah Abdullah","doi":"10.3390/biotech15030051","DOIUrl":"10.3390/biotech15030051","url":null,"abstract":"<p><p>Sophorolipids (SLs) produced from <i>Starmerella bombicola</i> using four different secondary substrates such as refined, bleached, and deodorized palm olein (RBD PO), RBD palm kernel olein (RBD PKO), RBD coconut olein (RBD CO) and fatty acid methyl ester (FAME) waste are reported. Their interfacial characteristics at medium-chain triglyceride (MCT) oil-water interface and ability to form nano/submicron emulsions were studied. The effects of SLs from different sources, SL concentrations and blend ratios of SLs and soybean lecithin on characteristics of emulsions produced by ultrasonication were examined. Initially, emulsion formed using SLs coded (from F2 to F5) showed large droplets (d₃₂ > 1000 nm) and poor stability. They were then blended with soybean lecithin at a ratio of 3:1 to produce emulsions coded F6 to F9 with smaller droplets (d₃₂ < 400 nm) and great stability over a range of temperatures (from 40 °C to 90 °C) and pH values (from 3 to 9). However, highly acidic (pH 2) and low ionic strength (1 mM NaCl) processing caused the separation of the emulsions. These emulsions also displayed potential antimicrobial activities towards <i>Bacillus cereus</i> and <i>Pseudomonas aeruginosa</i>, as well as cytotoxic effects against the human epithelial colorectal adenocarcinoma cell line (Caco-2). These results illustrated that stable emulsions required a mixture of SLs and soybean lecithin.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13397820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148580437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Next-Generation Metabolic Engineering of Capsaicinoids Biosynthesis in Chilli Pepper: Bridging Genomic Insights to Biotechnological Applications.","authors":"Thumadath Palayullaparambil Ajeesh Krishna, Deepa Harikrishnan, Mathew Veena, Theivanayagam Maharajan, M James, Minisha Udhayakumar, Parimala Gnana Soundari Arockiam Jeyasundar, Sherrie Jesulyn David, Ramar Dineshkumar, Reshma Rajan, Periyasamy Rathinapriya","doi":"10.3390/biotech15030050","DOIUrl":"10.3390/biotech15030050","url":null,"abstract":"<p><p>Chilli peppers (<i>Capsicum</i> species) have been widely used around the world because of their economic value and distinctive sensory characteristics. They contain abundant functional metabolites, especially a group of vanillylamide compounds belonging to the family of capsaicinoids, which have been exploited for medicinal, nutritional, agricultural, and cosmetic uses. The demand for capsaicinoid molecules is increasing day by day due to their high economic value and wide range of applications. Therefore, increasing bioactive metabolites, especially capsaicinoids in chilli peppers, is a major priority in the current scenario. Multi-omics approaches such as genomics, transcriptomics, proteomics, and metabolomics have substantially contributed to understanding the complex regulatory networks governing capsaicinoid biosynthesis. Key structural genes, transcription factors, and signaling pathways involved in the phenylpropanoid and branched-chain fatty acid pathways have been identified, providing valuable targets for metabolic engineering in chilli pepper. Despite these advances, the integration of genetic modification approaches for the targeted enhancement of capsaicinoid production remains limited in chilli pepper. Recent developments in biotechnology, particularly CRISPR/Cas-mediated genome-editing, enable the precise genetic modification of metabolic pathways and regulatory networks in plants. Therefore, it can contribute to the precise modification of key genes involved in the capsaicinoid biosynthesis pathway, offering potential strategies to enhance the capsaicinoid content in chilli pepper. However, CRISPR/Cas-mediated genome editing in chilli pepper is still in its early stages. There are currently no reports available on the successful enhancement of capsaicinoid content in chilli peppers through CRISPR/Cas-mediated genome editing. To date, no comprehensive review has evaluated the CRISPR-Cas-mediated genome-editing approaches for capsaicinoid metabolic engineering in chilli pepper. This review critically evaluates the recent advances in CRISPR/Cas-mediated metabolic engineering in chilli peppers, with particular emphasis on regulatory genes involved in capsaicinoid biosynthesis. Furthermore, multi-omics approaches are expected to complement these strategies by enabling the identification of key regulatory genes, the optimization of genome-editing targets, and the prediction of metabolic outcomes for enhanced capsaicinoid production. Overall, this review provides insights into improving capsaicinoid accumulation in chilli peppers through advanced genome-editing technologies.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13398006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148580356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}