IF 2.7

BioTech Pub Date : 2024-07-03 DOI: 10.3390/biotech13030023

Ana Filošević Vujnović, Sara Čabrijan, Martina Mušković, Nela Malatesti, Rozi Andretić Waldowski

{"title":"Systemic Effects of Photoactivated 5,10,15,20-tetrakis(N-methylpyridinium-3-yl) Porphyrin on Healthy Drosophila melanogaster","authors":"Ana Filošević Vujnović, Sara Čabrijan, Martina Mušković, Nela Malatesti, Rozi Andretić Waldowski","doi":"10.3390/biotech13030023","DOIUrl":"https://doi.org/10.3390/biotech13030023","url":null,"abstract":"Porphyrins are frequently employed in photodynamic therapy (PDT), a non-invasive technique primarily utilized to treat subcutaneous cancers, as photosensitizing agents (PAs). The development of a new PA with improved tissue selectivity and efficacy is crucial for expanding the application of PDT for the management of diverse cancers. We investigated the systemic effects of 5,10,15,20-tetrakis(N-methylpyridinium-3-yl)-porphyrin (TMPyP3) using Drosophila melanogaster adult males. We established the oral administration schedule and demonstrated that TMPyP3 was absorbed and stored higher in neuronal than in non-neuronal extracts. Twenty-four hours after oral TMPyP3 photoactivation, the quantity of hydrogen peroxide (H2O2) increased, but exclusively in the head extracts. Regardless of photoactivation, TMPyP3 resulted in a reduced concentration of H2O2 after 7 days, and this was linked with a decreased capacity to climb, as indicated by negative geotaxis. The findings imply that systemic TMPyP3 therapy may disrupt redox regulation, impairing cellular signaling and behavioral outcomes in the process. To determine the disruptive effect of porphyrins on redox homeostasis, its duration, and the mechanistic variations in retention across various tissues, more research is required.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141684141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Machine Learning-Based Web Tool for the Severity Prediction of COVID-19 基于机器学习的 COVID-19 严重性预测网络工具

IF 2.7

BioTech Pub Date : 2024-07-01 DOI: 10.3390/biotech13030022

Avgi Christodoulou, Martha-Spyridoula Katsarou, Christina Emmanouil, Marios Gavrielatos, Dimitrios Georgiou, Annia Tsolakou, M. Papasavva, Vasiliki V Economou, Vasiliki Nanou, Ioannis Nikolopoulos, Maria Daganou, A. Argyraki, Evaggelos Stefanidis, Gerasimos Metaxas, E. Panagiotou, Ioannis Michalopoulos, N. Drakoulis

{"title":"A Machine Learning-Based Web Tool for the Severity Prediction of COVID-19","authors":"Avgi Christodoulou, Martha-Spyridoula Katsarou, Christina Emmanouil, Marios Gavrielatos, Dimitrios Georgiou, Annia Tsolakou, M. Papasavva, Vasiliki V Economou, Vasiliki Nanou, Ioannis Nikolopoulos, Maria Daganou, A. Argyraki, Evaggelos Stefanidis, Gerasimos Metaxas, E. Panagiotou, Ioannis Michalopoulos, N. Drakoulis","doi":"10.3390/biotech13030022","DOIUrl":"https://doi.org/10.3390/biotech13030022","url":null,"abstract":"Predictive tools provide a unique opportunity to explain the observed differences in outcome between patients of the COVID-19 pandemic. The aim of this study was to associate individual demographic and clinical characteristics with disease severity in COVID-19 patients and to highlight the importance of machine learning (ML) in disease prognosis. The study enrolled 344 unvaccinated patients with confirmed SARS-CoV-2 infection. Data collected by integrating questionnaires and medical records were imported into various classification machine learning algorithms, and the algorithm and the hyperparameters with the greatest predictive ability were selected for use in a disease outcome prediction web tool. Of 111 independent features, age, sex, hypertension, obesity, and cancer comorbidity were found to be associated with severe COVID-19. Our prognostic tool can contribute to a successful therapeutic approach via personalized treatment. Although at the present time vaccination is not considered mandatory, this algorithm could encourage vulnerable groups to be vaccinated.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141714116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Critical Parameters on the Extraction of Concentrated C-PE from Thermotolerant Cyanobacteria. 关键参数对从耐热蓝藻中提取浓缩 C-PE 的影响

IF 2.7

BioTech Pub Date : 2024-06-24 DOI: 10.3390/biotech13030021

Ariadna H Vergel-Suarez, Janet B García-Martínez, German L López-Barrera, Néstor A Urbina-Suarez, Andrés F Barajas-Solano

{"title":"Influence of Critical Parameters on the Extraction of Concentrated C-PE from Thermotolerant Cyanobacteria.","authors":"Ariadna H Vergel-Suarez, Janet B García-Martínez, German L López-Barrera, Néstor A Urbina-Suarez, Andrés F Barajas-Solano","doi":"10.3390/biotech13030021","DOIUrl":"10.3390/biotech13030021","url":null,"abstract":"This work aimed to identify the influence of pH, molarity, w/v fraction, extraction time, agitation, and either a sodium (Na2HPO4·7H2O-NaH2PO4·H2O) or potassium buffer (K2HPO4-KH2PO4) used in the extraction of C-phycoerythrin (C-PE) from a thermotolerant strain of Potamosiphon sp. An experimental design (Minimum Run Resolution V Factorial Design) and a Central Composite Design (CCD) were used. According to the statistical results of the first design, the K-PO4 buffer, pH, molarity, and w/v fraction are vital factors that enhance the extractability of C-PE. The construction of a CCD design of the experiments suggests that the potassium phosphate buffer at pH 5.8, longer extraction times (50 min), and minimal extraction speed (1000 rpm) are ideal for maximizing C-PE concentration, while purity is unaffected by the design conditions. This optimization improves extraction yields and maintains the desired bright purple color of the phycobiliprotein.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11270330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141761417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing Bovine Embryo Development In Vitro Using Oil-in-Water Nanoemulsions as Specific Carriers for Essential Lipids 利用水包油型纳米乳液作为必需脂质的特异性载体促进牛胚胎体外发育

BioTech Pub Date : 2024-06-11 DOI: 10.3390/biotech13020019

Daniel Enrique López Angulo, R. Lourenço, A. Bridi, M. A. Chaves, J. D. da Silveira, P. J. Sobral

{"title":"Enhancing Bovine Embryo Development In Vitro Using Oil-in-Water Nanoemulsions as Specific Carriers for Essential Lipids","authors":"Daniel Enrique López Angulo, R. Lourenço, A. Bridi, M. A. Chaves, J. D. da Silveira, P. J. Sobral","doi":"10.3390/biotech13020019","DOIUrl":"https://doi.org/10.3390/biotech13020019","url":null,"abstract":"Worldwide meat consumption and production have nearly quintupled in the last 60 years. In this context, research and the application of new technologies related to animal reproduction have evolved in an accelerated way. The objective of the present study was to apply nanoemulsions (NEs) as carriers of lipids to feed bovine embryos in culture media and verify their impact on the development of embryos produced in vitro. The NEs were characterized by particle size, polydispersity, size distribution, physical stability, morphology using atomic force microscopy (AFM), surface tension, density, pH, and rheological behavior. The NEs were prepared by the emulsification/evaporation technique. A central composite rotatable design (CCRD) was used to optimize the NE fabrication parameters. The three optimized formulations used in the embryo application showed an emulsion stability index (ESI) between 0.046 and 0.086, which reflects high stability. The mean droplet diameter analyzed by laser diffraction was approximately 70–80 nm, suggesting a possible transit across the embryonic zona pellucida with pores of an average 90 nm in diameter. AFM images clearly confirm the morphology of spherical droplets with a mean droplet diameter of less than 100 nm. The optimized formulations added during the higher embryonic genome activation phase in bovine embryos enhanced early embryonic development.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141360734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spectrofluorimetric Analysis of Riboflavin Content during Kombucha Fermentation 昆布茶发酵过程中核黄素含量的荧光光谱分析

BioTech Pub Date : 2024-06-11 DOI: 10.3390/biotech13020020

M. Čakić Semenčić, A. Biedrzycka, Anna Kiczor, S. Beluhan, F. Šupljika

引用次数: 0

Phaseolus coccineus L. Landraces in Greece: Microsatellite Genotyping and Molecular Characterization for Landrace Authenticity and Discrimination 希腊的 Phaseolus coccineus L. 陆种：微卫星基因分型和分子特征描述促进陆地品系的真实性和鉴别性

BioTech Pub Date : 2024-06-07 DOI: 10.3390/biotech13020018

Irene Bosmali, G. Lagiotis, Ioannis Ganopoulos, Eleni Stefanidou, P. Madesis, C. Biliaderis

{"title":"Phaseolus coccineus L. Landraces in Greece: Microsatellite Genotyping and Molecular Characterization for Landrace Authenticity and Discrimination","authors":"Irene Bosmali, G. Lagiotis, Ioannis Ganopoulos, Eleni Stefanidou, P. Madesis, C. Biliaderis","doi":"10.3390/biotech13020018","DOIUrl":"https://doi.org/10.3390/biotech13020018","url":null,"abstract":"Phaseolus coccineus L. is a highly valuable crop for human consumption with a high protein content and other associated health benefits. Herein, 14 P. coccineus L. landraces were selected for genetic characterization: two Protected Geographical Indication (PGI) landraces from the Prespon area, namely “Gigantes” (“G”) and “Elephantes” (“E”), and 12 additional landraces from the Greek Gene Bank collection of beans (PC1–PC12). The genetic diversity among these landraces was assessed using capillary electrophoresis utilizing fluorescence-labeled Simple Sequence Repeat (SSR) and Expressed Sequence Tag (EST); Simple Sequence Repeat (SSR) is a molecular marker technology. The “G” and “E” Prespon landraces were clearly distinguished among them, as well as from the PC1 to PC12 landraces, indicating the unique genetic identity of the Prespon beans. Overall, the genetic characterization of the abundant Greek bean germplasm using molecular markers can aid in the genetic identification of “G” and “E” Prespon beans, thus preventing any form of fraudulent practices as well as supporting traceability management strategies for the identification of authenticity, and protection of the origin of local certified products.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141372612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simulation of Radiation-Induced DNA Damage and Protection by Histones Using the Code RITRACKS 使用 RITRACKS 代码模拟辐射诱导的 DNA 损伤和组蛋白的保护作用

BioTech Pub Date : 2024-06-05 DOI: 10.3390/biotech13020017

Ianik Plante, Devany W. West, Jason Weeks, Viviana I. Risca

{"title":"Simulation of Radiation-Induced DNA Damage and Protection by Histones Using the Code RITRACKS","authors":"Ianik Plante, Devany W. West, Jason Weeks, Viviana I. Risca","doi":"10.3390/biotech13020017","DOIUrl":"https://doi.org/10.3390/biotech13020017","url":null,"abstract":"(1) Background: DNA damage is of great importance in the understanding of the effects of ionizing radiation. Various types of DNA damage can result from exposure to ionizing radiation, with clustered types considered the most important for radiobiological effects. (2) Methods: The code RITRACKS (Relativistic Ion Tracks), a program that simulates stochastic radiation track structures, was used to simulate DNA damage by photons and ions spanning a broad range of linear energy transfer (LET) values. To perform these simulations, the transport code was modified to include cross sections for the interactions of ions or electrons with DNA and amino acids for ionizations, dissociative electron attachment, and elastic collisions. The radiochemistry simulations were performed using a step-by-step algorithm that follows the evolution of all particles in time, including reactions between radicals and DNA structures and amino acids. Furthermore, detailed DNA damage events, such as base pair positions, DNA fragment lengths, and fragment yields, were recorded. (3) Results: We report simulation results using photons and the ions 1H+, 4He2+, 12C6+, 16O8+, and 56Fe26+ at various energies, covering LET values from 0.3 to 164 keV/µm, and performed a comparison with other codes and experimental results. The results show evidence of DNA protection from damage at its points of contacts with histone proteins. (4) Conclusions: RITRACKS can provide a framework for studying DNA damage from a variety of ionizing radiation sources with detailed representations of DNA at the atomic scale, DNA-associated proteins, and resulting DNA damage events and statistics, enabling a broader range of future comparisons with experiments such as those based on DNA sequencing.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141385368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Silico and In Vitro Evaluation of the Antifungal Activity of a New Chromone Derivative against Candida spp. 新型铬酮衍生物对白色念珠菌抗真菌活性的硅学和体外评估

IF 2.7

BioTech Pub Date : 2024-05-25 DOI: 10.3390/biotech13020016

Gleycyelly Rodrigues Araújo, Palloma Christine Queiroga Gomes da Costa, Paula Lima Nogueira, Danielle da Nóbrega Alves, Alana Rodrigues Ferreira, Pablo R da Silva, Jéssica Cabral de Andrade, Natália F de Sousa, Paulo Bruno Araujo Loureiro, Marianna Vieira Sobral, Damião P Sousa, Marcus Tullius Scotti, Ricardo Dias de Castro, Luciana Scotti

{"title":"In Silico and In Vitro Evaluation of the Antifungal Activity of a New Chromone Derivative against Candida spp.","authors":"Gleycyelly Rodrigues Araújo, Palloma Christine Queiroga Gomes da Costa, Paula Lima Nogueira, Danielle da Nóbrega Alves, Alana Rodrigues Ferreira, Pablo R da Silva, Jéssica Cabral de Andrade, Natália F de Sousa, Paulo Bruno Araujo Loureiro, Marianna Vieira Sobral, Damião P Sousa, Marcus Tullius Scotti, Ricardo Dias de Castro, Luciana Scotti","doi":"10.3390/biotech13020016","DOIUrl":"10.3390/biotech13020016","url":null,"abstract":"Candida species are frequently implicated in the development of both superficial and invasive fungal infections, which can impact vital organs. In the quest for novel strategies to combat fungal infections, there has been growing interest in exploring synthetic and semi-synthetic products, particularly chromone derivatives, renowned for their antimicrobial properties. In the analysis of the antifungal activity of the compound (E)-benzylidene-chroman-4-one against Candida, in silico and laboratory tests were performed to predict possible mechanisms of action pathways, and in vitro tests were performed to determine antifungal activity (MIC and MFC), to verify potential modes of action on the fungal cell membrane and wall, and to assess cytotoxicity in human keratinocytes. The tested compound exhibited predicted affinity for all fungal targets, with the highest predicted affinity observed for thymidylate synthase (-102.589 kJ/mol). MIC and CFM values ranged from 264.52 μM (62.5 μg/mL) to 4232.44 μM (1000 μg/mL). The antifungal effect likely occurs due to the action of the compound on the plasma membrane. Therefore, (E)-benzylidene-chroman-4-one showed fungicidal-like activity against Candida spp., possibly targeting the plasma membrane.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11201913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141451795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of Wheat Bran by Co-Cultivation of Fungi with Integrated Hydrolysis to Provide Sugars and Animal Feed 通过联合培养真菌和综合水解来实现小麦麸皮的增值，从而提供糖分和动物饲料

BioTech Pub Date : 2024-05-18 DOI: 10.3390/biotech13020015

Fabian Mittermeier, Fabienne Fischer, Sebastian Hauke, Peter Hirschmann, Dirk Weuster-Botz

引用次数: 0

Challenges for the Post-Market Environmental Monitoring in the European Union Imposed by Novel Applications of Genetically Modified and Genome-Edited Organisms 转基因生物和基因组编辑生物的新应用对欧盟上市后环境监测带来的挑战

BioTech Pub Date : 2024-05-15 DOI: 10.3390/biotech13020014

M. Dolezel, Andreas Lang, Anita Greiter, Marianne Miklau, M. Eckerstorfer, A. Heissenberger, Eva Willée, W. Züghart

{"title":"Challenges for the Post-Market Environmental Monitoring in the European Union Imposed by Novel Applications of Genetically Modified and Genome-Edited Organisms","authors":"M. Dolezel, Andreas Lang, Anita Greiter, Marianne Miklau, M. Eckerstorfer, A. Heissenberger, Eva Willée, W. Züghart","doi":"10.3390/biotech13020014","DOIUrl":"https://doi.org/10.3390/biotech13020014","url":null,"abstract":"Information on the state of the environment is important to achieve the objectives of the European Green Deal, including the EU’s Biodiversity Strategy for 2030. The existing regulatory provisions for genetically modified organisms (GMOs) foresee an obligatory post-market environmental monitoring (PMEM) of potential adverse effects upon release into the environment. So far, GMO monitoring activities have focused on genetically modified crops. With the advent of new genomic techniques (NGT), novel GMO applications are being developed and may be released into a range of different, non-agricultural environments with potential implications for ecosystems and biodiversity. This challenges the current monitoring concepts and requires adaptation of existing monitoring programs to meet monitoring requirements. While the incorporation of existing biodiversity monitoring programs into GMO monitoring at the national level is important, additional monitoring activities will also be required. Using case examples, we highlight that monitoring requirements for novel GMO applications differ from those of GM crop plants previously authorized for commercial use in the European Union.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140976049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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