Environmental Advances最新文献

{"title":"Improvement in mixture risk assessment: A multi-national application of the Pesticide Risk Metric","authors":"Cath A. Neelamraju ,&nbsp;Reinier M. Mann ,&nbsp;Zoe T. Bainbridge ,&nbsp;Hannah C. Mitchell ,&nbsp;Ryan D.R. Turner","doi":"10.1016/j.envadv.2025.100676","DOIUrl":"10.1016/j.envadv.2025.100676","url":null,"abstract":"<div><div>Significant advancements have been made in the development of methods to assess mixture risk, with the hazard unit and concentration addition methods being commonly used for tier one screening risk assessments. However, a reduction in risk estimate uncertainty is required for higher tier assessments and targeted management action, particularly in the context of policy frameworks such as the European Green Deal, which aims to reduce the use and risk of pesticides that may pollute air, water and soil. This study proposes the Pesticide Risk Metric (PRM), an approach grounded in existing ecotoxicological methods for this purpose. By combining the species sensitivity distribution approach with the independent action model of joint toxicity, the PRM reduces uncertainty and provides more reliable risk estimates for individual toxicants and mixtures. The PRM method was applied to aqueous pesticide monitoring data from eight countries to demonstrate the utility of the method. Of the 706 sites, 61 (8.6 %) did not meet the annual average environmental quality standard (AA-EQS) for individual pesticide active ingredients, with Italy showing the highest number of exceedances. When assessed as a mixture, 359 sites (51 %) failed to meet the AA-EQS, with Italy and the Netherlands showing the highest annual average mixture toxicity. The primary contributors to mixture toxicity were insecticides (61 %), followed by other herbicides (32 %) and photosystem II inhibiting herbicides (6.1 %), with significant variation at the country and site levels. Fourteen pesticides contributed 99 % of the annual average mixture toxicity at the country level. The adaptability and reliable risk estimates of the PRM make it a valuable tool for second-tier risk assessment, further enhancing targeted policy and management decisions.</div></div>","PeriodicalId":34473,"journal":{"name":"Environmental Advances","volume":"22 ","pages":"Article 100676"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145519632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing physicochemical and biological indicators and their interactions in high-altitude lakes of Gokyo Valley, Nepal","authors":"Yadav Uprety ,&nbsp;Narayan Prasad Ghimire ,&nbsp;Binit Timalsina ,&nbsp;Susma Tamang ,&nbsp;Keshav Raj Pokharel","doi":"10.1016/j.envadv.2025.100675","DOIUrl":"10.1016/j.envadv.2025.100675","url":null,"abstract":"<div><div>The high-altitude Gokyo Lake Cluster (GLC) in eastern Nepal, part of the Hindu-Kush Himalayan region, represents a unique alpine freshwater ecosystem. Despite its Ramsar designation, little is known about the biotic and abiotic indicators and their interactions that govern its ecological structure and functions. This study examines the water chemistry, algal communities, benthic macroinvertebrates, and the relationships among these components across three lakes in the GLC. Water chemistry analysis revealed a dominance of Ca²⁺ &gt; Na⁺ &gt; Mg²⁺ &gt; K⁺ cations and an anion sequence of HCO₃⁻ &gt; Cl⁻ &gt; SO₄²⁻ &gt; NO₃⁻ &gt; PO₄³⁻. Piper and Gibbs plots indicated a mixed water type and a hydro-chemical regime dominated by rock weathering, primarily from carbonate and silicate lithologies. Macroinvertebrates represent 22 families belonging to 10 orders, where the Shannon diversity index ranged from 0.64 - 1.16. A total of 143 algal species from 80 genera were observed. Bacillariophyceae dominated (66.67 %) of the algal assemblage, indicating an oligotrophic lake, while Diptera and Ephemeroptera comprised 68.57 % and 17.86 % of macroinvertebrate abundance, respectively. The third lake exhibited the highest algal and invertebrate richness, potentially influenced by localized nutrient input from anthropogenic activities. Non-metric multidimensional scaling (NMDS) highlighted calcium hardness (r² = 0.620, p = 0.038) and temperature (r² = 0.671, p = 0.029) as significant environmental drivers of algal and macroinvertebrate community structure, respectively. These findings emphasize the sensitivity of alpine aquatic ecosystems to physicochemical gradients and human activities, underscoring the need for long-term monitoring and targeted management strategies, particularly given the area's Ramsar status within the protected area and increasing tourism pressure.</div></div>","PeriodicalId":34473,"journal":{"name":"Environmental Advances","volume":"22 ","pages":"Article 100675"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145466652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geochemical thresholds and bioavailability of potentially toxic elements in Amazonian soils: a risk-based framework for environmental management in mineralized watersheds","authors":"Mariana Maha Jana Costa de Figueiredo ,&nbsp;José Tasso Felix Guimarães ,&nbsp;Gabriel Negreiros Salomão ,&nbsp;Markus Gastauer ,&nbsp;Prafulla Kumar Sahoo ,&nbsp;Edilson Freitas da Silva ,&nbsp;Luiza Santos Reis ,&nbsp;Michelle Matos de Sousa ,&nbsp;Renato Oliveira da Silva Junior ,&nbsp;Pedro Walfir Martins e Souza-Filho ,&nbsp;Wendel Valter da Silveira Pereira ,&nbsp;Sílvio Junio Ramos","doi":"10.1016/j.envadv.2025.100667","DOIUrl":"10.1016/j.envadv.2025.100667","url":null,"abstract":"<div><div>This study evaluates the distribution, mobility, and lithological control of potentially toxic elements (PTE: As, Ba, Co, Cr, Cu, Hg, Mn, Mo, Ni, Pb, Sn, V, Zn), Al and Fe in surface soils of the Verde River Watershed (VRW), a highly mineralized and anthropogenically influenced basin within the Carajás Mineral Province (CMP), Brazilian Amazon. The CMP hosts world-class Fe ore reserves, large-tonnage IOCG (iron oxide–copper–gold), and Ni laterite deposits, while also being affected by small-scale Cu and Au mining, which contributes to regional environmental pressures. A total of 113 topsoil samples were analyzed for pseudo-total PTE (ICP–MS) and bioavailable (DTPA) fractions. Geochemical baselines were established using the modified median absolute deviation method, and multivariate analyses (clr-PCA, HCA), contamination indices (Igeo, EF, PLI, RAC), and chondrite-normalized REE patterns were applied to distinguish between geogenic enrichment and potential anthropogenic contributions. Results indicated that soil texture, mineralogy, and organic matter content are key drivers of PTE behavior. Soils developed over mafic–ultramafic lithologies in the CB showed higher clay and Fe/Al oxide content, promoting PTE retention. In contrast, felsic-derived soils in the CCD exhibited greater metal mobility, particularly for Cu, Zn, and Mn. RAC assessments identified Mn and Zn as the most mobile and potentially hazardous elements, while Fe remained largely immobile. The spatial variability of risk classifications aligned with lithological and pedogenic differences, underscoring the influence of geodiversity on contaminant dynamics. These findings contribute to a better understanding of metal behavior in tropical soil systems and offer a scientific basis for environmental monitoring and sustainable land management in mining-affected landscapes.</div></div>","PeriodicalId":34473,"journal":{"name":"Environmental Advances","volume":"22 ","pages":"Article 100667"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145098164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential benefits in all-cause and cause-specific mortality due to separate and joint hypothetical interventions of multiple air pollutants: A parametric g-computation analysis","authors":"Lan Chen ,&nbsp;Shengtao Wei ,&nbsp;Dashan Zheng ,&nbsp;Zhenhe Huang ,&nbsp;Kin-Fai Ho ,&nbsp;Fei Tian ,&nbsp;Weiyi Yang ,&nbsp;Wanqi Wen ,&nbsp;Zilong Zhang ,&nbsp;Hualiang Lin","doi":"10.1016/j.envadv.2025.100668","DOIUrl":"10.1016/j.envadv.2025.100668","url":null,"abstract":"<div><div>Although studies have demonstrated the adverse influence of air pollution, the optimal strategy for reducing air pollution levels to alleviate mortality burden remains elusive. With data from the UK Biobank, annual mean concentrations of five air pollutants (PM_2.5, PM₁₀, NO₂, NO_x, and SO₂) for each participant were estimated. The parametric g-computation was applied to estimate the risk differences (RDs) in all-cause and cause-specific mortality under various air pollution intervention strategies. We implemented both dynamic threshold interventions and percentage decremental interventions (e.g., 15%, 25% decrease in concentration) for each pollutant individually (separate strategies) and for multiple pollutants simultaneously (joint strategies). During a median follow-up of 11.51 years, 22,159 (5.98%) deaths occurred among the 370,357 participants. Both separate and joint air pollution intervention strategies were significantly associated with reduced risks of all-cause and cause-specific mortality, with all-cause mortality risk reduced by up to 2.0%. Notably, joint intervention strategies contributed to greater risk reductions compared to separate interventions. For example, the RDs for cancer mortality under the joint intervention scenarios were 1.3 to 3.4 times greater than those under separate interventions when the concentrations of PM_2.5, NO₂, and SO₂ were concurrently lowered by 35%. Our study indicates that targeting air pollution reduction could significantly reduce mortality risks. Joint intervention strategies are more highly recommended than separate intervention strategies.</div></div>","PeriodicalId":34473,"journal":{"name":"Environmental Advances","volume":"22 ","pages":"Article 100668"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145098165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the impact of fluctuating viral loads on virus recovery from electronegative membrane filters used for wastewater surveillance of biologicals","authors":"A.G. Shakallis ,&nbsp;H. Fallowfield ,&nbsp;K. Ross ,&nbsp;H. Whiley","doi":"10.1016/j.envadv.2025.100674","DOIUrl":"10.1016/j.envadv.2025.100674","url":null,"abstract":"<div><div>The emergence of SARS-CoV-2 hastened the adoption of wastewater-based epidemiology (WBE) for public health surveillance. While passive sampling results for SARS-CoV-2 appeared to agree with clinical sampling and infection numbers within the community, at the time there was limited investigation into factors that may affect the accuracy of results obtained through passive sampling. This study investigated the impact of fluctuating viral loads on virus recovery from a passive sampling material (electronegative membrane filters [EMFs]) using F-RNA bacteriophage MS2 as a model virus. Three models of fluctuating viral loads were tested: steady-state, substantial increase/decrease, and intermittent exposure. This resulted in eight concentration fluctuation scenarios. Experiments assessed whether concentration fluctuations impact virus recovery from EMFs, and whether there is an average or cumulative uptake of the exposed viral load. Findings showed that virus recovery reflected neither scenario, indicating that concentration fluctuations in wastewater impact the predictability of recovery from EMFs. Two predicted recovery models were calculated based on each recovery hypothesis, whether EMFs have an average or cumulative recovery. These models consistently under-predicted virus recovery during scenarios of increasing concentrations and over-predicted recovery during stable or decreasing concentrations. The measured recovery sometimes reflected the most recent concentration the samplers were exposed to rather than providing a reliable cumulative or average measurement, highlighting their limitations in accurately capturing dynamic or steady viral load conditions. This research enhances understanding of the complexities associated with using passive sampling for WBE, informing public health surveillance and policy development.</div></div>","PeriodicalId":34473,"journal":{"name":"Environmental Advances","volume":"22 ","pages":"Article 100674"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145466721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toward high-precision analysis of soil micro-and nanoplastics: A review of spectroscopy and machine learning approaches","authors":"Thi Kim Anh Tran ,&nbsp;Daniel Irving ,&nbsp;Wartini Ng ,&nbsp;Yijia Tang ,&nbsp;Thi Thanh Mai Nguyen ,&nbsp;Budiman Minasny ,&nbsp;Alex McBratney","doi":"10.1016/j.envadv.2025.100666","DOIUrl":"10.1016/j.envadv.2025.100666","url":null,"abstract":"<div><div>Micro-nanoplastics (MNPs) in soil have been recognised as a threat to soil and environment, impacting soil and food security, human health and ecosystem services. Current methods for analysing MNPs in soil are time consuming and expensive and hence cannot meet the growing need to quantify this threat on a larger scale. This review summarises existing knowledge on MNPs in soil and current analytical approaches. This includes examining the opportunities and challenges associated with various spectroscopy methods, such as visible–near-infrared (Vis-NIR), mid-infrared (MIR), Raman, terahertz (THz), and hyperspectral imaging (HSI), in detecting and quantifying MNPs in soil through machine learning enhanced analysis. While these methods show promise as cost-effective methods to facilitate routine analysis, key challenges persist. These include a lack of validation on naturally contaminated samples, difficulty detecting nanoplastics, a focus on classification rather than quantification, and detection limits that often exceed environmentally relevant concentrations. The review recommends expanding spectral libraries, enhancing resolution through spectral fusion and HSI, and integrating soil properties into machine learning models to improve detection accuracy.</div></div>","PeriodicalId":34473,"journal":{"name":"Environmental Advances","volume":"22 ","pages":"Article 100666"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145098163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations between rainfall and leaf area index-LAI and nutritional and health indicators in two districts of Southern Madagascar: The HydroNut project","authors":"Alan R. Patlán-Hernández ,&nbsp;Laure Ngabirano ,&nbsp;Myriam Ait Aissa ,&nbsp;Tom Heath ,&nbsp;Fara Pascale Rakotomandrindra ,&nbsp;Camille C. Ollivier ,&nbsp;Jean Lapegue ,&nbsp;Jacques Gardon ,&nbsp;Simon D. Carriere ,&nbsp;Dieynaba S. N'Diaye","doi":"10.1016/j.envadv.2025.100677","DOIUrl":"10.1016/j.envadv.2025.100677","url":null,"abstract":"<div><div>Climate crises affect human health, including nutritional status. However, evidence on this association remains limited. We investigated the associations between hydroclimatic indicators (rainfall and Leaf Area Index (LAI)) and health and nutrition outcomes (Severe Acute Malnutrition (SAM), diarrhea, Acute Respiratory Infections (ARI), Malaria) as part of the HydroNut project led by Action contre la Faim.</div><div>Time series data on monthly nutritional and health outcomes of children under five, along with hydroclimatic indicators, were collected for Amboasary (2014–2021) and Betioky (2016–2021) districts in Madagascar. Associations were assessed using negative binomial regression models adjusted for seasonality and trends providing the relative risk (RR) and its 95 % confidence interval (95 % CI). Lagged effects were also explored.</div><div>Data covered 54–90 months, depending on the indicator and district. In Amboasary and Betioky respectively, crude models showed positive associations of rainfall with SAM (RR[95 %CI]=1.005[1.002-1.007]; 1.003[1.000-1.005]), diarrhea (1.002[1.001-1.004]; 1.004[1.001-1.006]), and ARI only in Betioky (1.002[1.000-1.004]). Similar stronger associations were observed for LAI with SAM (3.138[2.174-4.530]; 2.122[1.504-2.994]), diarrhea (1.925[1.512-2.450]; 2.220[1.701-2.898]), and ARI in Betioky (1.813[1.368-2.401]). Overall, lag effects appeared within three months for rainfall and two months for LAI, showing a protective role between months 4–10 according to district and outcome. Malaria showed negative associations, turning positive after 4–7 months for rainfall and 2–6 months for LAI.</div><div>In conclusion, SAM, ARI, and diarrhea increased within three months of rainfall or two months of LAI increases. Further research is needed to better understand the mechanisms underlying these relationships, thereby improving humanitarian response strategies.</div></div>","PeriodicalId":34473,"journal":{"name":"Environmental Advances","volume":"22 ","pages":"Article 100677"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145614773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interannual analysis of noise levels and spectral trends in the marine soundscape of Kongsfjorden, Svalbard","authors":"Virginia Sciacca ,&nbsp;Francesco Paladini de Mendoza ,&nbsp;Giovanni de Vincenzi ,&nbsp;Maurizio Azzaro ,&nbsp;Federico Giglio ,&nbsp;Patrizia Giordano ,&nbsp;Leonardo Langone ,&nbsp;Stefano Miserocchi ,&nbsp;Francesco Filiciotto","doi":"10.1016/j.envadv.2025.100669","DOIUrl":"10.1016/j.envadv.2025.100669","url":null,"abstract":"<div><div>Until recently, the Arctic soundscape remained pristine due to low anthropogenic activity. Climate change and economic interests have rapidly increased underwater noise and may cause irreversible acoustic changes. Understanding environmental, biological, and anthropogenic components of the soundscape is key to predicting variations and impacts. This study presents the first interannual investigation of underwater noise levels at the innermost part of Kongsfjorden, Svalbard, a strategic hydrological and biological area. An autonomous recorder was deployed at 76 m and collected acoustic data between September 2021 and July 2023 (50 % duty cycle). Noise levels were analyzed using power spectral density and sound pressure level metrics in one-third octave frequency bands from 10 Hz to 10 kHz, at different temporal scales. Two main frequency cluster bands were identified, describing the distribution and variability of noise levels. Noise levels were lower in frequencies below 160 Hz, indicating minimal human-generated noise at the mooring site, and the greatest contribution to the spectrum was associated with the environmental component. Environmental variables showed complex and seasonally variable effects on the soundscape. Wind speed was identified as the primary driver, whilst air temperature and salinity contributed significantly during winter, likely due to their impact on water column stratification and acoustic propagation dynamics. Analysis revealed significant interannual differences in noise levels. These findings enhance our understanding of the sources and drivers of underwater sounds in this Arctic fjord, providing a foundation for long-term monitoring of the soundscape within Kongsfjorden.</div></div>","PeriodicalId":34473,"journal":{"name":"Environmental Advances","volume":"22 ","pages":"Article 100669"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145119946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of acute air pollution exposure on pregnant women's blood pressure: Insights from personal exposure assessment","authors":"Lorraine Barret ,&nbsp;Gaëlle Uzu ,&nbsp;Valérie Siroux ,&nbsp;Anouk Marsal ,&nbsp;Sarah Lyon-Caen ,&nbsp;Sam Bayat ,&nbsp;Rémy Slama ,&nbsp;Johanna Lepeule ,&nbsp;Marion Ouidir","doi":"10.1016/j.envadv.2025.100671","DOIUrl":"10.1016/j.envadv.2025.100671","url":null,"abstract":"<div><h3>Background</h3><div>Exposure to air pollution has been associated with adverse cardiovascular outcomes. Pregnant women may be especially vulnerable to air pollution due to physiological changes that affect blood pressure regulation. Traditional methods of estimating air pollution exposure based on home addresses and using either local monitoring stations, land use regression or dispersion models may not accurately capture individual exposure levels to explore its association with blood pressure.</div></div><div><h3>Objectives</h3><div>This study uses personal exposure to air pollutants to investigate short term associations with blood pressure outcomes at different stages of pregnancy.</div></div><div><h3>Method</h3><div>This study is based on data from the SEPAGES cohort. Blood pressure was measured during 3 visits throughout pregnancy, and air pollution exposure (benzene, toluene, ethylbenzene, o-xylene, m-xylene and p-xylene, nitrogen dioxide, fine particulates (PM_2.5), and PM_2.5 oxidative potential) was assessed using personal sensors during the week before blood pressure measurements. Multiple linear regressions were conducted for each outcome and pollutant, adjusting for confounding factors.</div></div><div><h3>Results</h3><div>478 pregnant women were included. Around the 19th week, we found a lower heart rate and pulse pressure associated with elevated PM_2.5 levels and their oxidative potential. In the later stages of pregnancy, we observed higher diastolic blood pressure and mean arterial pressure as PM_2.5 concentrations increased. Nonetheless, these findings should be interpreted cautiously, as no consistent pattern emerges across timeframes and pollutants.</div></div><div><h3>Conclusions</h3><div>Our study highlights the complex relationship between air pollution and blood pressure during pregnancy, suggesting various impacts depending on exposures and stage of pregnancy. Further research in larger cohorts using personal sensors is necessary to confirm these associations.</div></div>","PeriodicalId":34473,"journal":{"name":"Environmental Advances","volume":"22 ","pages":"Article 100671"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145268947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ice nucleation activity of two Antarctic sea ice bacteriophages PANV1 and OANV1 and the impact of freezing on their infectivity","authors":"Nina S. Atanasova ,&nbsp;Svetlana Sofieva-Rios ,&nbsp;Ari Laaksonen ,&nbsp;Rasmus Malmgren ,&nbsp;Linnea Mustonen ,&nbsp;Martin Romantschuk ,&nbsp;Aki Virkkula ,&nbsp;André Welti ,&nbsp;Ana A. Piedehierro","doi":"10.1016/j.envadv.2025.100659","DOIUrl":"10.1016/j.envadv.2025.100659","url":null,"abstract":"<div><div>Viruses constitute a special class of submicron-sized primary biological aerosol particles that are poorly characterized in terms of ice nucleation activity. Only a handful of studies have been performed about virus ice nucleation with the reported activity ranging between temperatures of approximately –14°C and –28°C. Because none of the previously known bacterial ice nucleation proteins are present in viruses, it has been hypothesized that the observed ice nucleation activity likely results from the high crystallinity and polydispersity of the overall structural organization of the virus particle and its subparts. Microbial ice nucleation has been suggested as a survival mechanism to tolerate cold atmospheric conditions but to date no reports exists whether virus infectivity persists during and after the ice nucleation process. In this work, we study the ice nucleation potential of two environmental viruses originating from Antarctic sea ice, PANV1 and OANV1 and describe the preservation of infectivity before and after the ice nucleation process. This is the first study that investigates the ice nucleating activity of viruses with an icosahedral head and a helical tail, which is one of the most common and abundant environmental virus morphotypes. It is possible that freezing in nature protects viruses and other biological ice nucleating particles, making this process an important mechanism contributing to the preservation of their viability in the environment.</div></div>","PeriodicalId":34473,"journal":{"name":"Environmental Advances","volume":"21 ","pages":"Article 100659"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}