Rossiiskii parazitologicheskii zhurnal最新文献

{"title":"Effects of Eimeria stiedae parasitism in the liver of rabbits on blood parameters and histoarchitecture of organs","authors":"V. Stafford","doi":"10.31016/1998-8435-2022-16-3-303-308","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-3-303-308","url":null,"abstract":"The purpose of the research is to evaluate the parasitism effects on clinical parameters of laboratory animals.Materials and methods. The venous blood of 20 rabbits aged 1 year was taken to perform biochemical and general blood tests, for which purpose hematological analyzers were used. The animals were dissected using the Shor’s method. The topography of the organ complex was evaluated, and pathological material was collected and placed to 10% buffered formalin. For pathomorphological examination, liver, spleen, lung, and kidney samples were taken. To determine the histological pattern, paraffin-embedded samples on Thermo Scientific semi-automatic equipment were used. Histologic specimens were stained with hematoxylin and eosin. The histoarchitecture of the specimens was evaluated using an Axio A1.0 microscope, and photography was conducted with the AxioVision software.Results and discussion. The article presents the data of the general and biochemical blood tests of the blood from the rabbits intended to be used in the experiment. Further, the pathoanatomical picture of the liver was shown in animals infected with Eimeria spp., and the histological pattern was presented for parenchymal organs. We found that the main biochemical values that exceeded reference values were liver values, namely, aspartate aminotransferase and alanine aminotransferase. We also observed an increase in monocytes and granulocytes in the blood. Pathological and anatomical changes were only expressed in the liver, while no changes in the macro pattern were observed in other organs. Histological examination of parenchymal organs showed a significant pathology in the liver due to endogenous stages of oocysts occurred in its structure. Additionally, we observed a strong eosinophil response in the spleen and a high content of eosinophils in the pulmonary veins.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41356530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The elimination period of ivermectin residuals from the body of broiler chickens after Iverbutan","authors":"E. Indyuhova, M. Arisov","doi":"10.31016/1998-8435-2022-16-3-309-318","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-3-309-318","url":null,"abstract":"The purpose of the research is to study the elimination period of Ivermectin residuals after three oral administrations of Iverbutan to broiler chickens.Materials and methods. For the experiment, 18 broiler chickens aged 28 days were selected. The birds were kept in the conditions of the Podolsk Experimental Production Department of the VNIIP – FSC VIEV. Iverbutan contains 0.4% of ivermectin and 10.0% of butaphosphan as active substances. The drug was administered orally by the group method at the rate of 1.0 mL of Iverbutan per 1 Liter of drinking water. Iverbutan was given three times: twice with a 24-hour interval and once after 14 days. The birds were killed and samples of their organs and tissues were taken at 9, 14, and 19 days after the drug was administered three times. Organs and tissues of each following type were collected from 6 birds: muscles, liver, kidneys, and skin with subcutaneous adipose tissue. The technique was based on the determination of Ivermectin by high performance liquid chromatography with modified pre-column accomplished with N-methylimidazole and trifluoroacetic anhydride, followed by fluorescence detection. The quantification was performed by the internal standardization.Results and discussion. The period was studied for elimination of Ivermectin residuals from the chickens’ body after the drug was administered three times. The residuals that exceed the maximum allowable levels were determined at 9 and 14 days after the drug was completed. It was found that the chickens’ organs and tissues did not contain Ivermectin at 19 days. Thus, poultry meat can be used for food at 19 days after Iverbutan is administered three times.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46243318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of syphaciosis on biochemical and clinical blood parameters of laboratory rats","authors":"N. B. Emelyanova, O. Kurnosova","doi":"10.31016/1998-8435-2022-16-3-296-302","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-3-296-302","url":null,"abstract":"The purpose of the research is to study the effect of syphaciosis on biochemical and clinical blood parameters of outbred rats.Materials and methods. Outbred male rats weighing 180–200 g were examined for helminth eggs by coproovoscopy and a Scotch tape test using a microscope Micromed 1 ver. 2-20. A biochemical blood assay was conducted on a Beckman Coulter DxC 700AU analyzer (USA), and a haematology test panel was made on a PCE 90-Vet analyzer (USA). Fenbendazole was used for preventive dehelminthization. Statistical processing was performed using the software Studet200.Results and discussion. The study results on biochemistry and hematology of the outbred rats’ blood showed a significant decrease in LDH levels and an increase in hematocrit in the animals infected with Syphacia spp.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45551178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of 2.0% Equiverm in high doses on the clinical state of the horses’ organism","authors":"M. Musaev, N. B. Emelyanova, E. Belova","doi":"10.31016/1998-8435-2022-16-3-319-326","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-3-319-326","url":null,"abstract":"The purpose of the research is to study the effect of the new antiparasitic 2.0% Equiverm in high doses on the clinical state of horses.Materials and methods. The experiment was conducted on 15 two-year-old crossbred horses weighing up to 300 kg spontaneously infected with Strongylata. To determine the effect of the antiparasitic paste on the horses, three groups of five horses each were formed. The first group of the horses was administered 2.0% Equiverm at a therapeutic dose; the second, at a three-fold increased dose, and the third, at a five-fold increased dose (0.2; 0.6 and 1.0 mg/kg for the active substance (AS), and 1.0; 3.0 and 5.0 mL per 100 kg of body weight for the drug). The horses’ clinical state was studied using standard methods. Blood samples for the study were taken from the jugular vein before the drug on the first, third and seventh days. The results obtained were statistically processed using the computer tool Microsoft Excel 2007.Results and discussion. It was found that the antiparasitic paste 2.0% Equiverm had no negative effect on clinical, hematological or biochemical parameters after a single oral administration at a therapeutic, three- and five-fold increased dose (0.2; 0.6 and 1.0 mg/kg for the AS, and 1.0; 3.0 and 5.0 mL per 100 kg of the body weight for the drug).","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43709567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effectiveness of methods to diagnose eimeriosis in turkeys on industrial farms and the species identification","authors":"E. Chalysheva, R. Safiullin","doi":"10.31016/1998-8435-2022-16-3-274-281","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-3-274-281","url":null,"abstract":"The purpose of the research is a comparative assessment of the effectiveness of methods to diagnose eimeriosis in turkeys and the species identification.Materials and methods. A comparative effectiveness assessment of life-time diagnostic methods for eimeriosis in turkey poults was conducted using coproscopic examinations: with sodium chloride alone, and with sodium chloride and glycerin according to Darling; with sodium chloride and glucose according to McMaster; and with sodium chloride alone according to Fülleborn. The diagnostic strength of different methods was evaluated with Eimeria oocysts artificially placed in standard litter samples free from infection. Morphological examinations and characteristics determination of Eimeria species in the turkey poults were conducted in the laboratory after the completed sporulation.Results and discussion. The diagnostic strength of the Fülleborn’s flotation method for turkey eimeriosis was 62.4%, 79.2% for the combined Darling1 methods, 85.6% for the combined Darling2 methods, and 90.4% for the McMaster’s methods. The combined Darling’s and McMaster’s methods used by us provide, according to their technology, for double centrifugation: water settling and flotation with saline, thus the microscopically examined sample contained not so many feed residues or other particles, which affected the diagnostic strength of the method. The young turkeys from the Penza and Moscow Regions’ farms were found to have the following types of Eimeria: Eimeria meleagrimitis in 62–80%, E. meleagridis in 15–16%, E. adenoides in 5–13%, and E. gallopavonis in 9%. E. meleagrimitis and E. meleagridis dominated on the turkey farms in the said regions. E. adenoides and E. gallopavonis were significantly less common.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44042804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digestive system of the hepatotrematode Parafasciolopsis fasciolaemorpha Ejsmont, 1932 (Plathelminthes, Trematoda): morphofunctional organization, histological and histochemical features to adaptive specialization","authors":"N. S. Manikovskaya, L. V. Nacheva","doi":"10.31016/1998-8435-2022-16-3-263-273","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-3-263-273","url":null,"abstract":"The purpose of the research is to study the morphofunctional organization, histological and histochemical features of the digestive system of the marita Parafasciolopsis fasciolaemorpha, which provide adaptation to parasitism in the endostasis – the liver Alces alces.Materials and methods. Mature specimens of the trematode P. fasciolaemorpha (Ejsmont, 1932) collected from the bile ducts of the Alces alces liver served as the material. Maritas were fixed in 70 and 80% alcohols, Schaffer alcohol-formalin 1 : 9, and 10% neutral formalin. Histological stains: with hematoxylin-eosin and by the Mallory method, followed by additional staining of the nuclei with Orta lithium carmine. Histochemical stains: sublimate-bromophenol blue according to Bonheg, Schick reaction according to McManus with additional staining of nuclei with Mayer's hemalaune, alcian blue according to Steedman and Mowry (pH 3.0 and 2.2) and toluidine blue (pH 2.0-5.0).Results and discussion. Parafasciolopsis fasciolaemorpha (Ejsmont, 1932) is the most pathogenic hepatoparasite of Alces alces L. The study of the features of the morphological organization of the digestive system as one of the border systems of the organism of the parasite revealed a number of features: the muscles of the large oral sucker contain many total proteins, which is confirmed by intense bromophenolophilia; in the thickness of the sucker wall there are small secretory cells and neurosecretory cells with abundant alcian- and toluidinophilic vacuolated cytoplasm; the presence of single and grouped digestive glands located in the parenchyma at the junction of the prepharynx to the pharynx, and on the border between the pharynx and the esophagus; in the apical part of the intestinal epithelium, a “brush border” is formed, the thin microvilli of which contribute to an increase in the working surface of parietal digestion in P. fasciolaemorpha, enhancing the trophic processes of the helminth to survive in the body owner. The revealed histological and histochemical features of the parafasciolopsis digestive system can be considered as examples of adaptive specialization at the site of endostasis.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42470777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The problem of anthelminthic resistance in horse breeding","authors":"O. Panova, I. Arkhipov, M. V. Baranova, A. Khrustalev","doi":"10.31016/1998-8435-2022-16-2-230-242","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-2-230-242","url":null,"abstract":"The purpose of the research is to outline the growing problem of anthelmintic resistance of horse nematodes in the world, to describe the methods currently used to detect it, and the proposed ways to overcome it.Materials and methods. A review of the world literature on the resistance of horse nematodes to anthelmintic drugs is carried out.Results and discussion. Currently, veterinary parasitology is faced with the growing problem of the emergence of resistant races of helminths, against which previously tested anthelmintic drugs in recommended doses are ineffective. This phenomenon has been noted in many animal species and manifests itself in relation to many drugs of the main groups of anthelmintics. This is evidenced by numerous reports. In horse breeding, benzimidazoles have been used for over 40 years, leading to widespread resistance to them in intestinal nematodes. There is a loss or weakening of the effectiveness of treatment of nematodes of horses with anthelmintic drugs: thiabendazole, pyrantel pamoat, drugs from the benzimidazole groups and macrocyclic lactones. There is no doubt that a similar situation of the spread of resistant races of helminths is also typical for Russia, since here the same anthelmintic drugs are used for treatment as abroad. However, this problem in our country remains practically unexplored. In this situation, the uncontrolled use of anthelmintics, which does not take into account the possibility of resistance to them, inevitably leads (and possibly in some cases has already led) to the emergence and spread of resistant populations of helminths, against which existing drugs will be ineffective.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46067088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modifcation of anthelmintic drugs by nanotechnology (review)","authors":"A. I. Varlamova, I. Arkhipov, S. Khalikov, M. Arisov","doi":"10.31016/1998-8435-2022-16-2-213-229","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-2-213-229","url":null,"abstract":"The purpose of the research is to analyze and summarize the literature data and the results of our own studies on the use of drug delivery systems and methods for increasing the solubility and efcacy of anthelmintic drugs.Helminthoses are one of the most important problems in veterinary medicine and animal husbandry, not only due to the pathological effects, but also due to the spread of anthelmintic resistance. Since the development of new anthelmintic substances takes many years and investment, some strategies are currently focused on modifying existing drugs to increase their efcacy, reduce side effects and overcome anthelmintic resistance. Literature data analysis on various methods and means of increasing the solubility and efcacy of anthelmintic drugs was carried out and the prospects for their use, including using modern nanotechnological methods of drug delivery were given.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47081622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of the 2% supramolecular complex of ivermectin in overdoses on horses","authors":"M. Musaev, V. V. Zaschepkina, S. Khalikov, A. Z. Dzhamalova","doi":"10.31016/1998-8435-2022-16-2-203-212","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-2-203-212","url":null,"abstract":"The purpose of the research is to study effects of the 2.0% supramolecular complex of ivermectin in overdoses on the clinical condition of horses.Materials and methods. The experiment was conducted on 20 horses of different breeds in the Seradin Equestrian Center, the North Caucasus Federal District, the Chechen Republic, in October 2020. The horses were divided according to the principle of analogues into 3 test groups and one control group of 5 animals each. The 2.0% supramolecular complex of ivermectin was administered to the horses from group 1 at a therapeutic dose of 0.2 mg/kg; group 2 received a tripled dose of 0.6 mg/kg, and the group 3, a 5-fold increased dose of 1,0 mg/kg for the active substance. The drug was administered once individually in a mixture with compound feed. The body temperature was measured with a non-contact thermometer, and the pulse rate and the respiratory rate were measured per 1 min. Urine of the horses was examined on days 1, 7 and 14 after the drug. The urine color, transparency and consistency were determined. Multifunctional test paper was used to study pH, protein, glucose, ketone bodies, nitrites, bilirubin, and urobilinogen. Blood was taken in the morning from the horses’ jugular vein on days 1, 7 and 14 after the drug. Hematological and biochemical parameters were studied on analyzers.Results and discussion. Clinical fndings of the horses (body temperature, pulse and respiration) did not differ from those of the control animals after the 2.0% supramolecular complex of ivermectin was administered in a therapeutic, tripled and 5-fold increased doses on days 1, 7 and 14. A slight increase in ketone bodies was observed in the urine on day 1, which may indicate the drug effect on the central nervous system at a 5-fold increased dose; all parameters were within the normal range on days 7 and 14. The 2.0% supramolecular complex of ivermectin in overdoses (0.6; 1.0 mg/kg for the active substance) did not adversely affect the horses’ hematological or biochemical blood parameters.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47210920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood parameters in dermatitis of various etiologies in cattle","authors":"D. A. Taranukha, B. Bagamaev, E. Gorchakov","doi":"10.31016/1998-8435-2022-16-2-185-192","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-2-185-192","url":null,"abstract":"The purpose of the research is to identify a general pattern in the change in hematological and biochemical parameters in any lesions of cattle skin.Materials and methods. The work was carried out in October-November 2021 in the conditions of farms in the Ipatovsky district of the Stavropol Territory on cattle aged 9–12 months and 90–120 kg of body weight. During the veterinary clinical examination, four groups of fve heads each were formed, one of the groups was a control. Animals of the control group had no clinical signs of dermatitis. The experimental groups of animals were divided depending on the degree of intensity of skin lesions into mild, moderate and generalized forms of manifestation of dermatitis. The results obtained were processed statistically.Results and discussion. A veterinary clinical examination of this livestock made it possible to establish the dependence in case of damage to the skin not only in changes in temperature, respiratory rate and pulse, but also in blood parameters. The body temperature of calves with a generalized form of dermatitis increased by an average of 0.5 degrees, breathing increased by six units per minute, the pulse rate increased by 15–18 beats per minute. In calves with mild and moderate manifestation of dermatitis, these parameters did not have signifcant changes and were within the normal range. From hematological indicators, hemoglobin had a general tendency to decrease with increasing intensity of the lesion or aggravation of the pathological process. Such indicators as ESR, leukocytes, neutrophils, eosinophils, on the contrary, tended to increase, especially in the generalized form. The slowdown of erythropoiesis and thrombocytopenia in the generalized form of dermatitis is presumably associated with intoxication of the body of calves. An increase in ESR, the number of eosinophils indicates an inflammatory process occurring in the animal's body. From biochemical indicators, an increase in the level of bilirubin, AST and ALT, creatinine, alkaline phosphatase indicates toxic liver damage due to intoxication of the body of calves due to inflammatory factors and decay products of affected skin cells.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48976561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}