Rossiiskii parazitologicheskii zhurnal最新文献_第5页

The efficacy of combined micronized Niclosamideand Albendazole-based dosage forms against bothriocephalosis of cyprinids 微粉硝沙美和阿苯达唑联合剂型对鲤双头病的疗效观察

Rossiiskii parazitologicheskii zhurnal Pub Date : 2023-01-02 DOI: 10.31016/1998-8435-2022-16-4-450-456

T. A. Vasilyeva, D. P. Skachkov

{"title":"The efficacy of combined micronized Niclosamideand Albendazole-based dosage forms against bothriocephalosis of cyprinids","authors":"T. A. Vasilyeva, D. P. Skachkov","doi":"10.31016/1998-8435-2022-16-4-450-456","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-4-450-456","url":null,"abstract":"The purpose of the research is to develop and test new combined dosage forms against bothriocephalosis of cyprinids in the cage culture fishery.Materials and methods. Combined micronized Niclosamide- and Albendazole-based dosage forms were developed; labscale batches of dosage forms of drugs and medicated feeds were developed with these drug forms; Bothriocephalus sp. infection rate in fish was determined during the growing period; and preliminary test effectiveness was confirmed for combined micronized niclosamide- and albendazole-based dosage forms against bothriocephalosis in cyprinids in the incubation shop of a cage culture fishery according to standard practices. The efficacy was assessed for combined micronized niclosamide- and albendazole-based dosage forms.Results and discussion. The preliminary tests of combined micronized Albendazole- and Niclosamide-based dosage forms as part of medicated feeds showed 100% extense-effectiveness with 100% intense-effectiveness, and 60% extenseeffectiveness with 77.8% intense-effectiveness when administered once at a dose of 5% of the fish weight (doses for the active substance of 50 and 40 mg/kg, respectively).","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46472965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Trichinella spiralis and Echinococcus multilocularis extracts after single and multiple injections on mitosis and hematological and biochemical parameters of mice 旋毛虫和多房棘球蚴单次和多次注射提取物对小鼠有丝分裂及血液生化指标的影响

Rossiiskii parazitologicheskii zhurnal Pub Date : 2023-01-02 DOI: 10.31016/1998-8435-2022-16-4-411-420

T. S. Novik, E. Koveshnikova, S. Chukina, L. Napisanova, O. Andreyanov, I. Arkhipov, K. Kurochkina

{"title":"Effects of Trichinella spiralis and Echinococcus multilocularis extracts after single and multiple injections on mitosis and hematological and biochemical parameters of mice","authors":"T. S. Novik, E. Koveshnikova, S. Chukina, L. Napisanova, O. Andreyanov, I. Arkhipov, K. Kurochkina","doi":"10.31016/1998-8435-2022-16-4-411-420","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-4-411-420","url":null,"abstract":"The purpose of the research is to study effects of Trichinella spiralis and Echinococcus multilocularis extracts on mitosis in a bone marrow cell population, and on hematological and biochemical blood parameters of mice after single and multiple intraperitoneal injections.Materials and methods. The experiment was conducted on outbred male mice. The T. spiralis and E. multilocularis extracts were administered intraperitoneally once or multiple times daily for 10 days at a dose of 80 μg/mouse. Bone marrow cell isolation, microscopic preparations, mitotic index and individual stage determination were made as described in the literature (Ford C. E., Hamerton J. L., 1956). The mouse main peripheral blood parameters were determined with a MicroCC-20 Plus hematological analyzer, and the leucogram was determined by a conventional method. Biochemical blood parameters of the mice were determined with a Clima MC-15 analyzer.Results and discussion. The T. spiralis and E. multilocularis extracts after a single intraperitoneal injection at a dose of 80 μg/ mouse had a pronounced negative effect on the mouse bone marrow cell mitosis with the cell division terminated in the metaphase and a decreased proportion of other mitosis stages. Characteristics were detected of the effects made by the T. spiralis and E. multilocularis extracts on the mouse bone marrow cell mitosis after multiple administration for 10 days. In the reinjection mode of the test extracts to the mice, hematological and biochemical parameters did not change.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46815642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Tolerability study of Imidacloprid-Pyriproxyfen-Moxidectin-based drugs in dogs and cats 吡虫啉-吡唑醚-莫西替丁类药物对猫狗的耐受性研究

Rossiiskii parazitologicheskii zhurnal Pub Date : 2023-01-02 DOI: 10.31016/1998-8435-2022-16-4-439-449

O. Tochieva, M. Arisov

{"title":"Tolerability study of Imidacloprid-Pyriproxyfen-Moxidectin-based drugs in dogs and cats","authors":"O. Tochieva, M. Arisov","doi":"10.31016/1998-8435-2022-16-4-439-449","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-4-439-449","url":null,"abstract":"The purpose of the research is to study tolerance of Imidacloprid-Pyriproxyfen-Moxidectin-based drugs for veterinary use by target animal species.Materials and methods. The research was conducted on 15 clinically healthy outbred cats aged 1–2 years weighing 3–5 kg, 15 clinically healthy outbred kittens aged 7–9 weeks weighing 700–900 g, 15 clinically healthy outbred dogs aged 1–2 years weighing 18–20 kg, and 15 clinically healthy outbred puppies aged 7 weeks up to three months weighing 6–9 kg, in the Kurilovo Experimental Base, the Podolsk Department of the VNIIP – FSC VIEV, in 2021. Combined drugs, Insacar Total S and Insacar Total K (Imidacloprid, Pyriproxyfen and Moxidectin), were used as drips, which was resulted in good absorption of active substances through the skin. The effect of combined Imidacloprid-Pyriproxyfen-Moxidectin-based drugs was evaluated on general condition and behavior, body weight gain dynamics, animal morphological and biochemical blood parameters, and physicochemical urine parameters.Results and discussion. Drugs for veterinary use, Insacar Total K and Insacar Total S, in increased therapeutic doses (twice and five-fold) do not adversely affect the general condition and behavior of cats, kittens, dogs or puppies; no deviations were recorded in morphological and biochemical blood parameters, or physicochemical urine parameters.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45361645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prophylactic efficacy of drugs against equine piroplasmidosis in Gorny Altai 阿尔泰省马螺旋体病药物预防效果观察

Rossiiskii parazitologicheskii zhurnal Pub Date : 2022-09-26 DOI: 10.31016/1998-8435-2022-16-3-359-366

V. A. Marchenko, V. Rar, I. Biryukov

{"title":"Prophylactic efficacy of drugs against equine piroplasmidosis in Gorny Altai","authors":"V. A. Marchenko, V. Rar, I. Biryukov","doi":"10.31016/1998-8435-2022-16-3-359-366","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-3-359-366","url":null,"abstract":"The purpose of the research is to study the prophylactic efficacy of piroplasmicides and to elucidate the effect of prophylactic doses of Imidocarb Dipropionate on the pathogen viability.Materials and methods. Babezan 12% was intramuscularly injected to 150 horses based on the active substance of Imidocarb Dipropionate at 2.5 mg/kg of the body weight and Neosidin M was injected to 30 horses twice at a dose of 2.5 mL per 100 kg of the live weight with a 15-day interval. The control group of animals did not receive the drug. The clinical follow-up of the experimental and control animals’ condition was done for 72 days. Before the experiment and 14 days after the drugs, blood samples were examined by the nested PCR in the presence of genus-specific primers from the 18S rRNA gene sequence for the Babesia spp. / Theileria spp. DNA. Species identification and genotyping of the detected Piroplasma were performed by sequencing 18S rRNA gene fragments.Results and discussion. Among 12 examined horses, 8 animals (66.7%) had the Piroplasmida DNA found in the blood samples, of which 50.0% were identified as Theileria equi and 16.7% as Babesia caballi. A high T. equi DNA prevalence (over 50.0%) indicates an endemic course of equine theileriosis. Babesan 12% in early chemotherapy of the horses helped to prevent the incidence of theileriosis within 48 and 59 days. Double chemotherapy of the horses with Neosidin M with a 15-day interval prevented the morbidity for 52 days. Babesan 12% at the preventive dose had no effect on the viability of persistent T. equi stages.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48405041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The effect of Farmayod on nematodes of different trophic groups in vitro and in vivo 法马洛德对不同营养类群线虫体外和体内的影响

Rossiiskii parazitologicheskii zhurnal Pub Date : 2022-09-26 DOI: 10.31016/1998-8435-2022-16-3-352-358

A. Konrat, T. S. Novik, A. Shesteperov

{"title":"The effect of Farmayod on nematodes of different trophic groups in vitro and in vivo","authors":"A. Konrat, T. S. Novik, A. Shesteperov","doi":"10.31016/1998-8435-2022-16-3-352-358","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-3-352-358","url":null,"abstract":"The purpose of the research is to study the effect of Farmayod on nematodes of different trophic groups, including rootknot nematode larvae, in vitro and in vivo.Materials and methods. The object of the research were larvae of the root-knot nematode Meloidogyne incognita obtained from the roots of infected plants from the Vladimir Region. The study of the effect of Farmayod in three concentrations was carried out in laboratory, using the biotest method on pumpkin plants. The effect of liquid Farmayod on the viability of nematodes of different trophic groups was studied in vitro and in vivo.Results and discussion. Liquid 0.1% Farmayod showed phytotoxicity, and not a single plant germinated. The drug in the form of a 0.01% solution showed phytotoxicity but to a lesser extent. The root system was less developed (60%) than in the control. The plant height was also 15% less. Farmayod at a concentration of 0.01% did not have phytotoxicity and reduced meloidoginosis versus the control. The biological efficacy of such dose was 56% higher, and the plant height was 30% more. The drug at a concentration of 0.01% had no effect on the plants damaged by meloidoginosis due to its phytotoxicity and poorly developed root system of the plants versus the control. Thus, at low concentrations, Farmayod acts on plants as a trace element necessary for plant vegetation, which affected the size of the plant. On the other hand, it can significantly reduce the infection of pumpkin roots with root-knot nematodes. Since this concentration is not toxic to nematodes, it can be assumed that the drug affects the nematode indirectly through the plant.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47377960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development issue of antiprotozoal agents to treat and prevent protozoal infections of fish, and theoretical and practical approaches to its solution 治疗和预防鱼类原生动物感染的抗原生动物药物的发展问题，以及解决该问题的理论和实践方法

Rossiiskii parazitologicheskii zhurnal Pub Date : 2022-09-26 DOI: 10.31016/1998-8435-2022-16-3-367-376

L. Fetisov, A. E. Svyatogorova, K. Kononenko, V. V. Chekrysheva, A. A. Zubenko

{"title":"Development issue of antiprotozoal agents to treat and prevent protozoal infections of fish, and theoretical and practical approaches to its solution","authors":"L. Fetisov, A. E. Svyatogorova, K. Kononenko, V. V. Chekrysheva, A. A. Zubenko","doi":"10.31016/1998-8435-2022-16-3-367-376","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-3-367-376","url":null,"abstract":"The purpose of the research is to determine safe parameters for the use of certain fatty acid amides against protozoal diseases of fish.Materials and methods. The synthesis and biological activity determination of new compounds were studied in laboratory conditions of the chemical synthesis creative team of the North Caucasus Zonal Research Veterinary Institute. The protistocidal activity of the new substances was determined in a liquid nutritional medium by serial dilution according to our technique. The ciliate Colpoda steinii was used as a test culture. The solution toxicity was determined on aquarium Guppy (Poecilia reticulata). The efficacy was assessed according to an alternative form of the response: the minimum concentration that all protozoan species died in was considered as protistocidal; the maximum concentration that all fish remained alive in was recognized as safe for the fish, i.e., the first concentration after the absolute lethal concentrations. Compliance with these conditions required the prepared solutions having minimal concentration ranges from 1 to 0.1 µg/mL, as well as continuous monitoring with recording the results. Two compounds were tested, namely, myristic acid amide and oleic acid amide.Results and discussion. The fish remained viable in the aqueous myristic acid amide solution concentration of 0.2 μg/mL for 3 days (monitoring period), and the fish remained alive for 3.5 hours or more (up to 20 hours) in the solution concentration of 0.5 μg/mL. At the same time, a concentration of 0.14 μg/mL had a protistocidal effect on the protozoan C. steinii if exposed for 40 minutes. The maximum tolerated oleic acid amide concentration for fish was 1 µg/mL if exposed for 2 or more days and 2 µg/mL if exposed for 4 h. The minimum protistocidal activity level of oleic acid amide was 0.58 µg/mL at an 18 h exposure.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43289113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Testing of micronized praziquantel and albendazole dosage forms against bothriocephalosis of cyprinids 微粉化吡喹酮和阿苯达唑剂型防治塞浦路斯双头病的试验

Rossiiskii parazitologicheskii zhurnal Pub Date : 2022-09-24 DOI: 10.31016/1998-8435-2022-16-3-341-351

T. A. Vasilyeva, D. P. Skachkov

{"title":"Testing of micronized praziquantel and albendazole dosage forms against bothriocephalosis of cyprinids","authors":"T. A. Vasilyeva, D. P. Skachkov","doi":"10.31016/1998-8435-2022-16-3-341-351","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-3-341-351","url":null,"abstract":"The purpose of the research is to develop and test new dosage forms against bothriocephalosis of cyprinids.Materials and methods. The study was performed in the experimental therapy laboratory of the VNIIP – FSC VIEV, and on a cage fish farm of Cherepetsky Rybkhoz JSC (Suvorov, Tula Region). A formulation was developed for two micronized dosage forms of albendazole and praziquantel to prevent and treat cestodosis in fish, namely, micronized albendazole and micronized praziquantel. For experiments to test micronized dosage forms of the drugs against bothriocephalosis of cyprinids, medicated feeds were developed using a special technology with added hot water and beet molasses. Each medicated feed contained 2% of the praziquantel- and albendazole-based dosage form. To determine the infection of the fish with cestodes, carp yearlings were studied at Cherepetsky Rybkhoz JSC. Clinical examination and pathoanatomical dissection of the fish were conducted selectively using a common method, after which the prevalence and intensity of the Bothriocephalus sp. infection were determined in fish. The medicated feed was given at the rate of 5% of fish weight in the trays, and put into the feeders installed at the bottom of the trays. The first group of fish received the medicated food with micronized albendazole, and the second, with micronized praziquantel. The doses were 25 and 30 mg/kg for the active substance, respectively. The effectiveness of experimental batches of the medicated feeds with praziquantel and albendazole dosage forms was recorded by results of the helminthological dissection of all experimental and control fish on day 4 of the therapeutic feeding.Results and discussion. Preliminary tests of the micronized albendazole and praziquantel dosage forms in the medicated feeds showed a 58.3% extense-effectiveness with a 61.9% intense-effectiveness, and a 75.0% extense-effectiveness with a 88.1% intense-effectiveness when applied once at a dose of 5% of fish weight (doses of 25 and 30 mg/kg for the AS, respectively).","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44134042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the efficacy of benzimidazole anthelmintics against different stages of gastrointestinal nematodes of young cattle 苯并咪唑驱虫剂对犊牛不同阶段胃肠道线虫的防治效果评价

Rossiiskii parazitologicheskii zhurnal Pub Date : 2022-09-24 DOI: 10.31016/1998-8435-2022-16-3-335-340

I. Arkhipov, A. I. Varlamova, A. Radionov

{"title":"Evaluation of the efficacy of benzimidazole anthelmintics against different stages of gastrointestinal nematodes of young cattle","authors":"I. Arkhipov, A. I. Varlamova, A. Radionov","doi":"10.31016/1998-8435-2022-16-3-335-340","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-3-335-340","url":null,"abstract":"The purpose of the research is to study the efficacy of benzimidazole anthelmintics against different development stages of gastrointestinal nematodes of young cattle.Materials and methods. The efficacy of benzimidazole drugs against early development stages of gastrointestinal nematodes was evaluated on 58 male calves aged 12–18 months spontaneously infected with gastrointestinal strongylates on the Moscow Region farms contaminated by nematode parasites. The animals were weighed, numbered and divided into experimental and control groups of 7–10 animals each. The male calves from different experimental groups were orally administered Panacur, Febtal, Fenbendazole (substance), Alben, Alvet, Valbazen, Closalben and Albendazole 10% powder once at a dose of 7.5 mg/kg for the active substance. The control animals did not receive the drug. The drug efficacy was recorded in the experiments of the \"control test\" type based on the coproovoscopic examination results by the flotation method using a VIGIS counting chamber, and on the results of helminthological dissections of the digestive tract of 3–5 animals from each group. The drug efficacy was recorded as per the Guidelines Approved by the World Association for the Advancement of Veterinary Parasitology (1995). The results were processed statistically using the Microsoft Excel computer tool.Results and discussion. We established the 94.4–97.2% efficacy of the drugs based on benzimidazoles, namely, Panacur, Febtal, Alben, Alvet, Valbazen and Closalben in therapeutic doses against imaginal gastrointestinal strongylates and 44.2–69.2% activity against nematode larvae.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43652182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of the acute toxicity and cumulative properties of the combined drug based on imidacloprid, pyriproxyfen and moxidectin 吡虫啉、吡唑醚和莫昔丁联合用药的急性毒性和累积特性研究

Rossiiskii parazitologicheskii zhurnal Pub Date : 2022-09-24 DOI: 10.31016/1998-8435-2022-16-3-327-334

O. Tochieva, M. Arisov

{"title":"Study of the acute toxicity and cumulative properties of the combined drug based on imidacloprid, pyriproxyfen and moxidectin","authors":"O. Tochieva, M. Arisov","doi":"10.31016/1998-8435-2022-16-3-327-334","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-3-327-334","url":null,"abstract":"The purpose of the research is the study of acute oral and dermal toxicity, and cumulative properties of the combined drug based on imidacloprid, pyriproxyfen and moxidectin on mice and rats.Materials and methods. The studies were conducted as provided by the Guidelines of the State PharmacologicalCommittee, in the VNIIP – FSC VIEV vivarium in 2021. We studied the acute oral and dermal toxicity, as well as cumulative properties of the combined drug in the form of a solution for external use that contains imidacloprid, pyriproxyfen and moxidectin as active substances. Outbred male mice and male rats were used in studying toxicological characteristics of the drug. General methods were used in studying the acute oral toxicity in the mice and rats, acute dermal toxicity in the rats and cumulative properties of the prototype product in the mice.Results and discussion. The LD 50 of the prototype product was 800 mg/kg of the animal weight when administered orally to the mice, and 2520±916.7 mg/kg, to the rats. Subject to the established median lethal doses, the drug was classified as the 3rd hazard class according to the general hygienic classification (GOST 12.1.007-76). When studying the acute dermal toxicity in the rats, the LD50 of the drug exceeded the maximum possible dose of 10,000 mg/kg. According to the general hygienic classification (GOST 12.1.007-76), the drug was classified as the 4th hazard class. The accumulation factor was 8.25, in which case the drug can be classified as the group of substances with weak cumulative activity.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42114000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bovine Leukemia Virus (BLV) isolates genetic analysis in perinatally infected cattle at young age 牛白血病病毒(BLV)分离遗传分析在围产期感染牛幼龄

Rossiiskii parazitologicheskii zhurnal Pub Date : 2022-09-23 DOI: 10.31016/1998-8435-2022-16-3-282-295

N. Kozyreva, I. Abashin, L. Ivanova

{"title":"Bovine Leukemia Virus (BLV) isolates genetic analysis in perinatally infected cattle at young age","authors":"N. Kozyreva, I. Abashin, L. Ivanova","doi":"10.31016/1998-8435-2022-16-3-282-295","DOIUrl":"https://doi.org/10.31016/1998-8435-2022-16-3-282-295","url":null,"abstract":"The purpose of the research is to identify perinatal infection in the dynamics, and assess the number and genetic status of bovine leukemia proviruses isolated from young animals, and correlations between some indicators of the infectious process based on gene diagnostics methods.Materials and methods. We used the material from cattle of different age groups: 1, calves (30–40 minutes after birth before colostrum and 15 to 45 days); and 2, heifers (not older than two years). Radial immunodiffusion (RID), real-time polymerase chain reaction (PCR), and phylogenetic analysis were used. Results and discussion. An assessment is given for perinatal infection of the young cattle. The detection rate of the infection in the calves was 4.15% (PCR) and 1.09% (RID); and 1.1% (PCR) and 0.88% (RID) in the heifers. A 36-fold decrease of the infection was found in positive dynamics (2013–2022) from 14.5 to 0.4% with passing through 0% (2020) and being at the level of 0% (2022). The proviral load ranged from 2.02 × 104 to 8.38 × 106 GE/mL in the blood of the examined animals. The BLV isolates obtained were shown to belong to two genotypes, GIV and GVII (env), and clade 1 (pol). We assessed an overestimation of the number of the proviruses by a factor of three in the animals under two years of age (3.83 × 106GE/mL) relative to that in the 1-month-old calves (1.3 × 106 GE/mL), and by a factor of nine for GIV relative to GVII. It is important to develop gene diagnostics algorithms to increase the effectiveness of routine tools to prevent the spread of this retrovirus infection in young animals at an early stage, which is confirmed by a decrease to 0% of detected retrovirus infection in young animals over time. The provirus number was higher in the heifers than the calves; the proviral load level was higher in the multiparous dairy cows than the nulliparous animals, and quantitative indicators were higher in the animals’ blood with the GIV genotype relative to those with the GVII genetic variant of the BLV.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45335189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0