治疗和预防鱼类原生动物感染的抗原生动物药物的发展问题,以及解决该问题的理论和实践方法

L. Fetisov, A. E. Svyatogorova, K. Kononenko, V. V. Chekrysheva, A. A. Zubenko
{"title":"治疗和预防鱼类原生动物感染的抗原生动物药物的发展问题,以及解决该问题的理论和实践方法","authors":"L. Fetisov, A. E. Svyatogorova, K. Kononenko, V. V. Chekrysheva, A. A. Zubenko","doi":"10.31016/1998-8435-2022-16-3-367-376","DOIUrl":null,"url":null,"abstract":"The purpose of the research is to determine safe parameters for the use of certain fatty acid amides against protozoal diseases of fish.Materials and methods. The synthesis and biological activity determination of new compounds were studied in laboratory conditions of the chemical synthesis creative team of the North Caucasus Zonal Research Veterinary Institute. The protistocidal activity of the new substances was determined in a liquid nutritional medium by serial dilution according to our technique. The ciliate Colpoda steinii was used as a test culture. The solution toxicity was determined on aquarium Guppy (Poecilia reticulata). The efficacy was assessed according to an alternative form of the response: the minimum concentration that all protozoan species died in was considered as protistocidal; the maximum concentration that all fish remained alive in was recognized as safe for the fish, i.e., the first concentration after the absolute lethal concentrations. Compliance with these conditions required the prepared solutions having minimal concentration ranges from 1 to 0.1 µg/mL, as well as continuous monitoring with recording the results. Two compounds were tested, namely, myristic acid amide and oleic acid amide.Results and discussion. The fish remained viable in the aqueous myristic acid amide solution concentration of 0.2 μg/mL for 3 days (monitoring period), and the fish remained alive for 3.5 hours or more (up to 20 hours) in the solution concentration of 0.5 μg/mL. At the same time, a concentration of 0.14 μg/mL had a protistocidal effect on the protozoan C. steinii if exposed for 40 minutes. The maximum tolerated oleic acid amide concentration for fish was 1 µg/mL if exposed for 2 or more days and 2 µg/mL if exposed for 4 h. The minimum protistocidal activity level of oleic acid amide was 0.58 µg/mL at an 18 h exposure.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development issue of antiprotozoal agents to treat and prevent protozoal infections of fish, and theoretical and practical approaches to its solution\",\"authors\":\"L. Fetisov, A. E. Svyatogorova, K. Kononenko, V. V. Chekrysheva, A. A. Zubenko\",\"doi\":\"10.31016/1998-8435-2022-16-3-367-376\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The purpose of the research is to determine safe parameters for the use of certain fatty acid amides against protozoal diseases of fish.Materials and methods. The synthesis and biological activity determination of new compounds were studied in laboratory conditions of the chemical synthesis creative team of the North Caucasus Zonal Research Veterinary Institute. The protistocidal activity of the new substances was determined in a liquid nutritional medium by serial dilution according to our technique. The ciliate Colpoda steinii was used as a test culture. The solution toxicity was determined on aquarium Guppy (Poecilia reticulata). The efficacy was assessed according to an alternative form of the response: the minimum concentration that all protozoan species died in was considered as protistocidal; the maximum concentration that all fish remained alive in was recognized as safe for the fish, i.e., the first concentration after the absolute lethal concentrations. Compliance with these conditions required the prepared solutions having minimal concentration ranges from 1 to 0.1 µg/mL, as well as continuous monitoring with recording the results. Two compounds were tested, namely, myristic acid amide and oleic acid amide.Results and discussion. The fish remained viable in the aqueous myristic acid amide solution concentration of 0.2 μg/mL for 3 days (monitoring period), and the fish remained alive for 3.5 hours or more (up to 20 hours) in the solution concentration of 0.5 μg/mL. At the same time, a concentration of 0.14 μg/mL had a protistocidal effect on the protozoan C. steinii if exposed for 40 minutes. The maximum tolerated oleic acid amide concentration for fish was 1 µg/mL if exposed for 2 or more days and 2 µg/mL if exposed for 4 h. The minimum protistocidal activity level of oleic acid amide was 0.58 µg/mL at an 18 h exposure.\",\"PeriodicalId\":34353,\"journal\":{\"name\":\"Rossiiskii parazitologicheskii zhurnal\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-09-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Rossiiskii parazitologicheskii zhurnal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.31016/1998-8435-2022-16-3-367-376\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rossiiskii parazitologicheskii zhurnal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31016/1998-8435-2022-16-3-367-376","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

本研究的目的是确定使用某些脂肪酸酰胺对抗鱼类原生动物疾病的安全参数。材料和方法。在北高加索地区兽医研究所化学合成创新团队的实验室条件下,对新化合物的合成和生物活性测定进行了研究。根据我们的技术,在液体营养培养基中通过连续稀释测定了新物质的杀质子活性。使用纤毛虫Colpoda steinii作为试验培养物。在水族箱Guppy(Poecilia reticulata)上测定了溶液毒性。疗效根据另一种反应形式进行评估:所有原生动物物种死亡的最低浓度被认为是杀原生动物的;所有鱼类存活的最大浓度被认为对鱼类是安全的,即绝对致死浓度之后的第一个浓度。符合这些条件需要制备的溶液的最小浓度范围为1至0.1µg/mL,并通过记录结果进行连续监测。测试了两种化合物,即肉豆蔻酸酰胺和油酸酰胺。结果和讨论。该鱼在浓度为0.2μg/mL的肉豆蔻酸酰胺水溶液中存活3天(监测期),在浓度为0.5μg/mL溶液中存活3.5小时或更长时间(最多20小时)。同时,0.14μg/mL的浓度暴露40分钟对斯坦尼氏原生动物有杀卵作用。如果暴露2天或2天以上,鱼类的最大耐受油酸酰胺浓度为1µg/mL,如果暴露4小时,则为2µg/mL。暴露18小时时,油酸酰胺的最小杀卵活性水平为0.58µg/mL。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development issue of antiprotozoal agents to treat and prevent protozoal infections of fish, and theoretical and practical approaches to its solution
The purpose of the research is to determine safe parameters for the use of certain fatty acid amides against protozoal diseases of fish.Materials and methods. The synthesis and biological activity determination of new compounds were studied in laboratory conditions of the chemical synthesis creative team of the North Caucasus Zonal Research Veterinary Institute. The protistocidal activity of the new substances was determined in a liquid nutritional medium by serial dilution according to our technique. The ciliate Colpoda steinii was used as a test culture. The solution toxicity was determined on aquarium Guppy (Poecilia reticulata). The efficacy was assessed according to an alternative form of the response: the minimum concentration that all protozoan species died in was considered as protistocidal; the maximum concentration that all fish remained alive in was recognized as safe for the fish, i.e., the first concentration after the absolute lethal concentrations. Compliance with these conditions required the prepared solutions having minimal concentration ranges from 1 to 0.1 µg/mL, as well as continuous monitoring with recording the results. Two compounds were tested, namely, myristic acid amide and oleic acid amide.Results and discussion. The fish remained viable in the aqueous myristic acid amide solution concentration of 0.2 μg/mL for 3 days (monitoring period), and the fish remained alive for 3.5 hours or more (up to 20 hours) in the solution concentration of 0.5 μg/mL. At the same time, a concentration of 0.14 μg/mL had a protistocidal effect on the protozoan C. steinii if exposed for 40 minutes. The maximum tolerated oleic acid amide concentration for fish was 1 µg/mL if exposed for 2 or more days and 2 µg/mL if exposed for 4 h. The minimum protistocidal activity level of oleic acid amide was 0.58 µg/mL at an 18 h exposure.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
38
审稿时长
8 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信