Annals of Clinical Microbiology最新文献_第9页

Evaluating the Use of Distilled Water for Washing Sodium Hydroxide in Mycobacterial Culture 用蒸馏水洗涤分枝杆菌培养中氢氧化钠的效果评价

Annals of Clinical Microbiology Pub Date : 2020-01-01 DOI: 10.5145/acm.2020.23.4.5

Hae-Kyong Back, Kozar' Mi, M. Lee

引用次数: 0

Cellulitis Caused by a Novel Cupriavidus Species Strain J1218 Identified by Whole Genome Sequencing 全基因组测序鉴定一株铜斑菌J1218引起蜂窝织炎

Annals of Clinical Microbiology Pub Date : 2019-12-01 DOI: 10.5145/acm.2019.22.4.105

Y. Ko, Soo-Young Yoon, C. Lim, C. Lee

引用次数: 1

Antimicrobial Resistance in Bacterial Isolates Recovered from Nursing Hospitals between 2014 and 2017 2014年至2017年间疗养院回收的细菌分离株的耐药性

Annals of Clinical Microbiology Pub Date : 2019-12-01 DOI: 10.5145/acm.2019.22.4.96

S. Yun, Bareum Gwon, H. L. Hong, Hwan Seop Lim, K. Lee, I. Jang, Eun-Jeong Yoon, S. Jeong

{"title":"Antimicrobial Resistance in Bacterial Isolates Recovered from Nursing Hospitals between 2014 and 2017","authors":"S. Yun, Bareum Gwon, H. L. Hong, Hwan Seop Lim, K. Lee, I. Jang, Eun-Jeong Yoon, S. Jeong","doi":"10.5145/acm.2019.22.4.96","DOIUrl":"https://doi.org/10.5145/acm.2019.22.4.96","url":null,"abstract":"Background: Antimicrobial resistance (AMR) is an issue not only with regard to public health, but also in terms of economic impact. AMR surveillance has mainly been carried out in general hospitals, and not in nursing hospitals. This study was conducted to investigate the AMR rate for bacterial strains isolated from nursing hospital samples. Methods: Antimicrobial susceptibility testing (AST) results from a total of 23,518 bacterial isolates recovered from clinical specimens taken in 61 nursing hosals were analyzed. AST was conducted using Vitek 2 with AST cards specific for the bacterial strains. Results: A total of 19,357 Gram-negative and 4,161 Gram-positive bacterial strains were isolated. Pseudomonas aeruginosa (n=6,384) and Escherichia coli (n= 5,468) were the most prevalent bacterial species and, among Gram-positive bacteria, Staphylococcus aureus (n=1,565) was common. The AMR rate was high for the following strains: cefotaxime-resistant Klebsiella pneumoniae, 77.4%; cefotaxime-resistant E. coli, 70.6%; imipenem-resistant Acinetobacter baumannii, 90.3%; imipenem-resistant P. aeruginosa, 49.3%; oxacillin-resistant S. aureus, 81.1%, penicillin-resistant Enterococcus faecalis, 44.8%, and vancomycin-resistant Enterococcus faecium, 53.5%. AMR rate change varied by bacterial species and antimicrobial drug. Conclusion: AMR rates of major pathogens from nursing hospitals were higher than those from general hospitals with the exception of imipenem-resistant A. baumannii. Continuous monitoring and infection control strategies are needed. (Ann Clin Microbiol 2019;22:96-104)","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43305541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of BacT/Alert FAN Plus Bottles for the Culture of Peritoneal Dialysate BacT/Alert FAN Plus瓶用于腹膜透析液培养的评价

Annals of Clinical Microbiology Pub Date : 2019-12-01 DOI: 10.5145/acm.2019.22.4.90

Min-Seung Park, I. Yoo, O. Kang, Jung Eun Lee, D. Kim, H. Huh, N. Lee

{"title":"Evaluation of BacT/Alert FAN Plus Bottles for the Culture of Peritoneal Dialysate","authors":"Min-Seung Park, I. Yoo, O. Kang, Jung Eun Lee, D. Kim, H. Huh, N. Lee","doi":"10.5145/acm.2019.22.4.90","DOIUrl":"https://doi.org/10.5145/acm.2019.22.4.90","url":null,"abstract":"Background: A major complication of peritoneal dialysis (PD) is peritonitis, and bacterial culture of PD effluent in a blood culture bottle is the preferred technique for diagnosis of peritonitis. In this study, we compared dialysate inoculation and culture using the BacT/AlerTR Fastidious Antimicrobial Neutralization Plus blood culture bottles (FAN Plus; bioMérieux, France) to the conventional centrifugation culture method. Methods: A total of 170 PD effluents were simultaneously processed by the conventional centrifugation culture method and by culture using FAN Plus media with two different inoculation procedures: inoculation after centrifugation and direct bedside inoculation. Results: Of the 52 cultures that were positive on at least one of the culture methods, 27 samples were positive on conventional centrifugation. However, 46 samples showed growth following inoculation into the FAN Plus media after centrifugation, and 47 samples were positive on the direct FAN Plus inoculation method. Using the case definition for PD peritonitis to classify samples, sensitivity of the conventional method was 50.0% (95% CI, 33.7-66.3%), whereas the sensitivity of the FAN Plus media was 78.9% (95% CI, 62.2-89.9%) by inoculation after centrifugation and 86.8% (95% CI, 71.1-95.1%) by direct inoculation. Use of both inoculation methods with FAN Plus media resulted in 92.1% sensitivity (95% CI, 89.2-99.9%). Conclusion: Culture using FAN Plus media demonstrated a superior bacterial recovery rate to the conventional centrifugation culture method. A combination of the two inoculation methods with FAN Plus media is recommended for the best diagnostic yield, while direct inoculation alone can be useful due to its simplicity and cost-effectiveness. (Ann Clin Microbiol 2019;22:90-95)","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45730726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance Evaluation of Newly Developed Korean Antimicrobial Susceptibility Testing Panels for MicroScan System Using Clinical Isolates from Teaching Hospitals in Korea 使用韩国教学医院临床分离物的微扫描系统最新开发的韩国抗菌药物敏感性测试板的性能评估

Annals of Clinical Microbiology Pub Date : 2019-09-01 DOI: 10.5145/acm.2019.22.3.61

Dayoung Kang, J. Hong, Byeol Yi Park, I. Jang, Dokyun Kim, Hyukmin Lee, S. Jeong

{"title":"Performance Evaluation of Newly Developed Korean Antimicrobial Susceptibility Testing Panels for MicroScan System Using Clinical Isolates from Teaching Hospitals in Korea","authors":"Dayoung Kang, J. Hong, Byeol Yi Park, I. Jang, Dokyun Kim, Hyukmin Lee, S. Jeong","doi":"10.5145/acm.2019.22.3.61","DOIUrl":"https://doi.org/10.5145/acm.2019.22.3.61","url":null,"abstract":"Background: Antimicrobial resistant continues to pose a threat to public health. Therefore, rapid and accurate antimicrobial susceptibility testing is very important. The objectives of this study were to evaluate the performance of the MicroScan system (Beckman Coulter, USA) with newly developed Korean Antimicrobial Susceptibility Testing Panels (KSCM panels) for antimicrobial susceptibility testing (AST) against clinical isolates in South Korea. Methods: Three KSCM panels were designed in this study. For the performance evaluation, a total of 1,325 clinical isolates including 1,027 of Gram-negative bacilli and 298 Gram-positive cocci collected from eight general hospitals in South Korea were used. The results by KSCM panels were compared with those by conventional methods. Results: By KSCM-1 panel for Gram-positive cocci, the rates of categorical agreement (CA) were >90% in all the antimicrobials tested in this study. The rates of major error (ME) were also <3%, and only three very major error (VME) were identified; each of ampicillin, tetracycline, and quinupristin-dalfopristin in enterococcal isolates. By KSCM-2 panel for Enterobacteriaceae, the rates of CA were also above 90%, and those of ME and VME were less than 3% and 1.5%, respectively. KSCM-3 panels for glucose-non-fermenting Gram-negative bacilli, also showed good agreement rates, i.e., CA rates >90%, ME rates <3%, and VME rates <1.5%. Conclusion: The newly developed three KSCM panels for MicroScan system (Beckman Coulter) showed excellent performance in AST against a large number of clinical isolates, and they are applicable to clinical microbiology laboratories. (Ann Clin Microbiol 2019;22:61-70)","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5145/acm.2019.22.3.61","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49162080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Staphylococcus pettenkoferi Isolated from Blood Culture 从血培养物中分离得到的pettenkoferi葡萄球菌的鉴定

Annals of Clinical Microbiology Pub Date : 2019-09-01 DOI: 10.5145/acm.2019.22.3.77

Hyein Kang, N. Ryoo

引用次数: 2

Serotyping and Antimicrobial Susceptibility of Salmonella Isolated in Korea in 2015 2015年韩国分离的沙门氏菌的血清分型和耐药性

Annals of Clinical Microbiology Pub Date : 2019-09-01 DOI: 10.5145/acm.2019.22.3.55

S. Kim, E. Park, I. Hwang, Hyukmin Lee, S. Song, Miae Lee, Seungok Lee, Soo-young Kim, Jin Ju Kim, Jongcheol Shin, S. Hong, K. Shin, Sunjoo Kim, N. Ryoo, Woonhyoung Lee, S. Jang, J. Shin

{"title":"Serotyping and Antimicrobial Susceptibility of Salmonella Isolated in Korea in 2015","authors":"S. Kim, E. Park, I. Hwang, Hyukmin Lee, S. Song, Miae Lee, Seungok Lee, Soo-young Kim, Jin Ju Kim, Jongcheol Shin, S. Hong, K. Shin, Sunjoo Kim, N. Ryoo, Woonhyoung Lee, S. Jang, J. Shin","doi":"10.5145/acm.2019.22.3.55","DOIUrl":"https://doi.org/10.5145/acm.2019.22.3.55","url":null,"abstract":"Department of Clinical Laboratory Science, Semyung University, Jecheon, Department of Infectious Disease, Busan Institute of Health and Environment, Busan, Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Department of Laboratory Medicine, Inje University College of Medicine, Busan, Department of Laboratory Medicine, Ewha Womans University College of Medicine, Seoul, Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Department of Laboratory Medicine, Inha University College of Medicine, Incheon, Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Department of Laboratory Medicine, Chungbuk National University College of Medicine, Cheongju, Department of Laboratory Medicine, Gyeongsang National University College of Medicine, Jinju, Department of Laboratory Medicine, Keimyung University College of Medicine, Daegu, Department of Laboratory Medicine, Kosin University College of Medicine, Busan, Department of Laboratory Medicine, College of Medicine, Chosun University, Gwangju, Paik Institute for Clinical Research, Inje University, Busan, Korea","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5145/acm.2019.22.3.55","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43408833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Prevalence and Species Spectrum of Pulmonary Nontuberculous Mycobacteria Isolates at a Tertiary Care Center 某三级保健中心肺非结核分枝杆菌的流行及种类谱分析

Annals of Clinical Microbiology Pub Date : 2019-09-01 DOI: 10.5145/acm.2019.22.3.71

Youngsung Joo, N. Kwak, Gun Han Kim, Eun-Jeong Yoon, S. Jeong

{"title":"Prevalence and Species Spectrum of Pulmonary Nontuberculous Mycobacteria Isolates at a Tertiary Care Center","authors":"Youngsung Joo, N. Kwak, Gun Han Kim, Eun-Jeong Yoon, S. Jeong","doi":"10.5145/acm.2019.22.3.71","DOIUrl":"https://doi.org/10.5145/acm.2019.22.3.71","url":null,"abstract":"Background: Pulmonary infection with nontuberculous mycobacteria (NTM) is increasing in South Korea. Since treatment strategy differs by NTM species, ac-curate identification is necessary. In this study, using Mycobacterium pulmonary isolates recently recovered from a general hospital in Seoul, the prevalence of NTM isolates was investigated. Methods: A total of 483 Mycobacterium pulmonary strains isolated between May and November 2018 from an 814-bed general hospital in South Korea were analyzed. Bacterial species were identified based on nucleotide sequences of the 16S-23S rDNA internal transcribed spacer and the rpoB gene. Results: From a total of 1,209 pulmonary specimens from patients suspected to be infected with mycobacteria, 324 deduplicate strains were isolated, compris-ing 90 Mycobacterium tuberculosis and 229 NTM strains. Among the NTM isolates, 61.5% (n=144) were Mycobacterium avium complex (MAC), including 92 M. avium and 52 Mycobacterium intracellulare , while 8.1% (n=19) represented Mycobacterium abscessus , including 10 M. abscessus subsp. abscessus and 9 M. abscessus subsp. massiliense . In addition, 12 (5.1%) Mycobacterium lentiflavum , 12 (5.1%) Mycobacterium gordonae , 6 (2.6%) Mycobacterium kansasii , and 5 (2.1%) Mycobacterium fortuitum were identified. In addition, Mycobacterium mucogenicum (n=2), Mycobacterium septicum (n=1), Mycobacterium colombiens (n=1), Mycobacterium asiaticum (n=1), and Mycobacterium celatum (n=1) were identified. Conclusion: Among the recently recovered Mycobacterium pulmonary strains, more than half were identified as NTM, and MAC was the most prevalent NTM, followed by M. . (Ann","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44952922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Comparison of Multiplex Real-Time Polymerase Chain Reaction Assays for Detection of Respiratory Viruses in Nasopharyngeal Specimens 多重实时聚合酶链反应法检测鼻咽标本中呼吸道病毒的比较

Annals of Clinical Microbiology Pub Date : 2019-06-01 DOI: 10.5145/ACM.2019.22.2.35

Uwizeyimana Jean Damascene, Kim Min-kyung, Kim Dae-won, Byun Jung-Hyun, Yong Dong-Eun

{"title":"Comparison of Multiplex Real-Time Polymerase Chain Reaction Assays for Detection of Respiratory Viruses in Nasopharyngeal Specimens","authors":"Uwizeyimana Jean Damascene, Kim Min-kyung, Kim Dae-won, Byun Jung-Hyun, Yong Dong-Eun","doi":"10.5145/ACM.2019.22.2.35","DOIUrl":"https://doi.org/10.5145/ACM.2019.22.2.35","url":null,"abstract":"Background: Respiratory tract infections are major public health threats, and the identification of their causative microbes helps clinicians to initiate timely and appropriate antimicrobial therapy and prevent the secondary spread of infection. The main goal of this study was to compare two multiplex real-time polymerase chain reaction (PCR) assays used to detect respiratory viral pathogens in nasopharyngeal swab specimens. Methods: Between September and October 2017, a total of 84 nasopharyngeal specimens were obtained consecutively from patients in a tertiary hospital using a flocked swab with 3 mL universal transport medium (COPAN Diagnostics, USA). A total of 64 positive and 20 negative sample results from the LG AdvanSure RV real-time RT-PCR kit (LG Life Sciences, Korea) were further retested using a new AdvanSure RV-plus a real-time RT-PCR kit to compare their performance. Results: Statistical analysis of positive and negative agreement between the two different kits was conducted between the newly introduced AdvanSure RV-plus real-time RT-PCR kit and the AdvanSure RV real-time RT-PCR. The overall agreement was 96.4%, with positive agreement of 98.4% and negative agreement of 90%. The evaluated sensitivity and specificity of AdvanSure RV-plus real-time RT-PCR were 96.9% and 94.7%, respectively, with a kappa value of 0.9 ( P ＜ 0.001). Conclusion: The performances of LG AdvanSure RV real-time RT-PCR and the new AdvanSure RV-plus real-time RT-PCR kit showed strong overall agreement. AdvanSure RV-plus real-time RT-PCR had a better detection rate and could detect coronavirus 229E and enterovirus, especially with a high detection rate in coinfection. AdvanSure RV-plus real-time RT-PCR can be considered a useful tool for respiratory virus diagnosis in clinical laboratories. (Ann Clin Microbiol 2019;22:35-41)","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5145/ACM.2019.22.2.35","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47077902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antimicrobial Susceptibility and Genetic Analysis of Campylobacter jejuni Isolated from Diarrhea Patients in Busan 釜山地区腹泻患者空肠弯曲杆菌的药敏及遗传分析

Annals of Clinical Microbiology Pub Date : 2019-06-01 DOI: 10.5145/ACM.2019.22.2.42

S. Park, Byeong Jun Kim, Young-Hee Kwon, I. Hwang, Gyung-hye Sung, E. Park, S. Jin

{"title":"Antimicrobial Susceptibility and Genetic Analysis of Campylobacter jejuni Isolated from Diarrhea Patients in Busan","authors":"S. Park, Byeong Jun Kim, Young-Hee Kwon, I. Hwang, Gyung-hye Sung, E. Park, S. Jin","doi":"10.5145/ACM.2019.22.2.42","DOIUrl":"https://doi.org/10.5145/ACM.2019.22.2.42","url":null,"abstract":"Background: Campylobacter jejuni is an important food-borne pathogen that causes human gastroenteritis. This study was conducted to investigate the incidence of isolation, antimicrobial susceptibility pattern, and C. jejuni genotype from diarrhea patients in Busan, Korea. Methods: A total of 97 C. jejuni were isolated from diarrhea patients during five food-borne outbreaks from 2014 to September 2017. Antimicrobial susceptibility tests were carried out by the broth microdilution method for ciprofloxacin (CIP), nalidixic acid (NAL), tetracycline (TET), chloramphenicol, azithromycin (AZI), erythromycin (ERY), streptomycin (STR), gentamicin, and telithromycin. To investigate C. jejuni genotypes, pulsed-field gel electrophoresis (PFGE) profile analysis was performed. Results: The isolation rate of C. jejuni was 2.0% for the last 4 years and increased annually. Antimicrobial resistance rates of C. jejuni were shown to be in the order of NAL (90.9%), CIP (89.4%), TET (13.6%), AZI (3.0%), ERY (3.0%), and STR (1.5%). The proportion of multidrug-resistance was 18.2%, and they commonly contained quinolones (CIP-NAL). Analysis of PFGE patterns of SmaI-restricted DNA of C. jejuni isolates showed 17 clusters; cluster 11 was the major genotype pattern. Conclusion: This study will provide useful data for the proper use of antimicrobials and the management of resistant C. jejuni. Also it will help to provide data for the epidemiological investigation of foodborne diseases caused by C. jejuni, which is expected to increase in the future. (Ann Clin Microbiol 2019;22:42-49)","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5145/ACM.2019.22.2.42","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46040784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3