Annals of Clinical Microbiology最新文献_第10页

Performance Evaluation of SENTiFIT 270 and FOB Gold Reagent for Detecting Fecal Occult Blood SENTiFIT 270与FOB金试剂检测粪便潜血的性能评价

Annals of Clinical Microbiology Pub Date : 2019-06-01 DOI: 10.5145/acm.2019.22.2.29

Dayoung Kang, Dokyun Kim, Keonhan Kim, I. Jang, S. Jeong

{"title":"Performance Evaluation of SENTiFIT 270 and FOB Gold Reagent for Detecting Fecal Occult Blood","authors":"Dayoung Kang, Dokyun Kim, Keonhan Kim, I. Jang, S. Jeong","doi":"10.5145/acm.2019.22.2.29","DOIUrl":"https://doi.org/10.5145/acm.2019.22.2.29","url":null,"abstract":"Background: Fecal occult blood tests have been widely used to screen for colorectal cancer. SENTiFIT 270 (Sentinel diagnostics, Italy) is a fecal occult blood test with an immunochemical method that utilizes FOB Gold reagents. We evaluated the performance of SENTiFIT 270 using the FOB Gold reagent. In addition, FOB Gold was evaluated with the HITACHI 7180 (Hitachi Ltd., Japan). Methods: The precision and linearity of the SENTiFIT 270 was evaluated in accordance with applicable Clinical and Laboratory Standard Institute guidelines. The comparison study between SENTiFIT 270-FOB Gold and the OC-Sensor (Eiken chemical Co., Japan) was performed using stool specimens. Results: In the precision evaluation, the total precision of SENTiFIT 270-FOB Gold was 4.94% and 2.54% at high and low concentrations, respectively. The HITACHI 7180-FOB Gold had excellent precision of 4.60% and 2.09% at high and low concentrations, respectively. Linearity was also excellent for the SENTiFIT 270-FOB Gold and HITACHI 7180-FOB Gold at 0.9987 and 0.9986, respectively. The SENTITIF 270-FOB Gold showed excellent agreement with a kappa value of 0.830 and a concordance rate of 93.6%. The HITACHI 7180-FOB Gold showed high agreement with a kappa value of 0.832 and a concordance rate of 93.9%. Conclusion: The SENTiFIT 270-FOB Gold showed excellent performance in accuracy, linearity, and com-parative inspection ability. (Ann Clin Microbiol 2019; 22:29-34)","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5145/acm.2019.22.2.29","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42238922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facklamia hominisIsolated from a Wound: A Case Report and Review of the Literature 从创伤中分离的人面神经炎病例报告及文献复习

Annals of Clinical Microbiology Pub Date : 2019-06-01 DOI: 10.5145/acm.2019.22.2.50

Tae Yeul Kim, J. Jo, Namhee Kim, Hyunwoong Park, E. Roh, J. Yoon, Sue Shin

引用次数: 1

Genetic Variants and Haplotypes in the IL10 Gene and Their Association with Opportunistic Infections among HIV-Infected Patients in Korea in the Era of Highly Active Antiretroviral Therapy 在高效抗逆转录病毒治疗时代，IL10基因的遗传变异和单倍型及其与韩国hiv感染者机会性感染的关系

Annals of Clinical Microbiology Pub Date : 2019-03-01 DOI: 10.5145/ACM.2019.22.1.14

I. Kim, H. Kim, Chulhun L. Chang

{"title":"Genetic Variants and Haplotypes in the IL10 Gene and Their Association with Opportunistic Infections among HIV-Infected Patients in Korea in the Era of Highly Active Antiretroviral Therapy","authors":"I. Kim, H. Kim, Chulhun L. Chang","doi":"10.5145/ACM.2019.22.1.14","DOIUrl":"https://doi.org/10.5145/ACM.2019.22.1.14","url":null,"abstract":"Background: Genetic variants and haplotypes of the interleukin-10 ( IL10 ) gene have been shown to affect clinical outcomes, including the incidence of opportunistic infections (OIs), in HIV-infected patients. This study investigated the effect of IL10 gene variants on susceptibility to OIs in HIV-infected Korean patients in the era of highly active antiretroviral therapy (HAART). Methods: Eighty-five HIV-infected patients receiving HAART were enrolled in the study. OIs were diagnosed based on the published criteria of the Korean Society for AIDS. Three promoter SNPs and four haplotype-tagging SNPs (htSNPs) of IL10 were selected and genotyped. The haplotypes were re-constructed according to the genotyping data and linkage disequilibrium (LD) status of these SNPs. Results: During the study, 38 OIs developed in 23 of the 85 patients (27.1%), at a rate of 1.7 episodes/ patient. Carrying the minor alleles at the rs1518111, rs3024490, and rs1800871 SNPs had a protective effect against OIs (adjusted P =0.035). Among the seven assessed variants, only three possible haplotypes were observed. The second most common haplotype, which was composed of the rs1518111 minor allele and the rs3021094 major allele showed a protective effect against OIs ( P =0.0153). Conclusion: This study demonstrated that some IL10 genetic variants and haplotypes are associated with protective effects against OIs in the era of HAART. These data suggest the potential of two htSNPs, rs1518111 and rs3021094, as markers revealing the genetic association of IL10 in Koreans. This is the first report on the association of IL10 with OIs in HIV-infected Korean patients in the era of HAART. (Ann Clin Microbiol 2019;22:14-22)","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5145/ACM.2019.22.1.14","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44632993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial Endocarditis Caused byAbiotrophia defectivain a Healthy Adult: A Case Report with Literature Review 健康成人无营养缺陷所致细菌性心内膜炎1例并文献复习

Annals of Clinical Microbiology Pub Date : 2019-03-01 DOI: 10.5145/ACM.2019.22.1.23

H. Je, Duyeal Song, Chulhun L. Chang

{"title":"Bacterial Endocarditis Caused byAbiotrophia defectivain a Healthy Adult: A Case Report with Literature Review","authors":"H. Je, Duyeal Song, Chulhun L. Chang","doi":"10.5145/ACM.2019.22.1.23","DOIUrl":"https://doi.org/10.5145/ACM.2019.22.1.23","url":null,"abstract":"Infective endocarditis caused by Abiotrophia defectiva is rarely encountered. A 67-year-old male transferred from a local hospital presented with severe dyspnea and pulmonary edema. Preoperative transthoracic echocardiography revealed severe mitral regurgitation with large vegetation. Blood cultures grew A. defectiva , a gram positive, nutritionally deficient streptococcus variant. Emergent mitral valve replacement through right thoracotomy was performed, and after completing six weeks of antibiotic combination therapy (vancomycin, ampicillin, and gentamicin), the patient recovered fully. Because of the need for prompt surgical treatment and long-term antibiotic therapy and lack of laboratory experience with the organism, physicians and laboratory workers should pay close attention to the possibility of A. defectiva infective endocarditis when gram positive cocci are detected in blood cultures. (Ann Clin Microbiol 2019;22:23-27) multiple foci in both cerebral hemi-spheres and left cerebellum, and subarachnoid hemorrhage (SAH) along both parietal and right occipital sulci. Blood cultures were requested and ceftriaxone and vancomycin were started empirically. To prevent further embolism by the cardiac vegetation, emergent mitral valve replacement was conducted through right mini-thoracotomy. Intraoperative findings showed massive destruction of anterior and posterior mitral valve with huge to posterior of the mitral to posterior atrial","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41570818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Development and Evaluation of Multiplex PCR for the Detection of Carbapenemase-Producing Enterobacteriaceae 检测产碳青霉烯酶肠杆菌科细菌的多重PCR方法的建立与评价

Annals of Clinical Microbiology Pub Date : 2019-03-01 DOI: 10.5145/ACM.2019.22.1.9

S. Kim, I. K. Bae, Na Young Kim, S. Song, Sunjoo Kim, Joseph Jeong, J. Shin

{"title":"Development and Evaluation of Multiplex PCR for the Detection of Carbapenemase-Producing Enterobacteriaceae","authors":"S. Kim, I. K. Bae, Na Young Kim, S. Song, Sunjoo Kim, Joseph Jeong, J. Shin","doi":"10.5145/ACM.2019.22.1.9","DOIUrl":"https://doi.org/10.5145/ACM.2019.22.1.9","url":null,"abstract":"Background: The isolation of carbapenemase-produc-ing Enterobacteriaceae (CPE) has become increas-ingly common. Continuous surveillance for these or-ganisms is essential because their infections are closely related to outbreaks of illness and are asso-ciated with high mortality rates. The aim of this study was to develop and evaluate multiplex PCR as a means of detecting several important CPE genes simultaneously. Methods: We aimed to develop a multiplex PCR that could detect seven CPE genes simultaneously. The multiplex PCR was composed of seven primer sets for the detection of KPC, IMP, VIM, NDM-1, GES, OXA-23, and OXA-48. We designed different PCR product sizes of at least 100 bp. We evaluated the performance of this new test using 69 CPE-positive clinical isolates. Also, we confirmed the specificity to rule out false-positive reactions by using 71 carbape-nem-susceptible clinical strains. Results: A total of 69 CPE clinical isolates showed positive results and were correctly identified as KPC (N=14), IMP (N=13), OXA-23 (N=12), OXA-48 (N=11), VIM (N=9), GES (N=5), and NDM (N=5) by the multiplex PCR. All 71 carbapenem-susceptible clinical isolates, including Enterococcus faecalis , Escherichia coli , Klebsiella pneumoniae , Acinetobacter baumannii , and Pseudomonas aeruginosa , showed negative results. Conclusion: This multiplex PCR can detect seven CPE genes at a time and will be useful in clinical laboratories. (Ann Clin Microbiol 2019;22:9-13)","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5145/ACM.2019.22.1.9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47005864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Differences in Antimicrobial Resistance Phenotypes by the Group of CTX-M Extended-Spectrum β-Lactamase CTX-M扩展谱β-内酰胺酶群的耐药表型差异

Annals of Clinical Microbiology Pub Date : 2019-03-01 DOI: 10.5145/ACM.2019.22.1.1

Bareum Gwon, Eun-Jeong Yoon, Dokyun Kim, Hyukmin Lee, Jong-Hee Shin, J. Shin, K. Shin, Y. A. Kim, Y. Uh, H. S. Kim, Young Ree Kim, S. Jeong

引用次数: 0

Comparison of PANA RealTyper HPV Kit with AdvanSure HPV GenoBlot Assay for Human Papillomavirus Genotyping PANA RealTyper HPV检测试剂盒与AdvanSure HPV GenoBlot检测人乳头瘤病毒基因分型的比较

Annals of Clinical Microbiology Pub Date : 2018-12-01 DOI: 10.5145/ACM.2018.21.4.86

Y. Kim, Hae-Sun Chung, Miae Lee

{"title":"Comparison of PANA RealTyper HPV Kit with AdvanSure HPV GenoBlot Assay for Human Papillomavirus Genotyping","authors":"Y. Kim, Hae-Sun Chung, Miae Lee","doi":"10.5145/ACM.2018.21.4.86","DOIUrl":"https://doi.org/10.5145/ACM.2018.21.4.86","url":null,"abstract":"Background: The PANA RealTyper HPV kit (PANAGENE, Korea; PANA RealTyper) was developed to genotype human papillomavirus (HPV) and was based on multiplex real-time PCR amplification and melting curve analysis. In this study, we compared PANA RealTyper to the AdvanSure HPV GenoBlot assay (LG Life Sciences, Korea; AdvanSure assay) and attempted to evaluate the performance of PANA RealTyper. Methods: A total of 60 cervical specimens were collected from women undergoing routine cervical cancer screening. The AdvanSure assay and PANA RealTyper kit identified the same 20 high-risk genotypes. However, the AdvanSure assay identified 15 low-risk genotypes, while the PANA RealTyper kit identified only 2 but detected 18 low-risk genotypes. Results: Among the total 60 specimens, 54 high-risk genotypes (40 specimens) and 20 low-risk genotypes (18 specimens) were detected. The agreement rates of the assays ranged from 94.4 to 100% for high-risk genotypes. Among 9 genotypes that were positive in the PANA RealTyper kit but negative in the AdvanSure assay, 7 were confirmed as true positive (HPV genotypes 16 (n=1), 39 (n=1), 52 (n=1), 58 (n=2), 68 (n=2)). Among 4 genotypes that were negative in the PANA RealTyper kit but positive in the AdvanSure assay, 3 were confirmed as HPV genotype 59. Among the 19 low-risk genotypes positive in the AdvanSure assay, there were 2 cases of HPV 6 and 1 case of HPV 11. In comparison, only 1 positive case of HPV 6 was determined by the PANA RealTyper kit. Conclusion: The PANA RealTyper kit was comparable with the AdvanSure assay. The PANA RealTyper kit would be useful and suitable for HPV genotyping in the clinical laboratory. (Ann Clin Microbiol 2018;21:8691)","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":"21 1","pages":"86-91"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5145/ACM.2018.21.4.86","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48480573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nocardia abscessus Cutaneous Abscess: A Case Report and Review of the Literature 脓肿诺卡菌皮肤脓肿1例报告及文献复习

Annals of Clinical Microbiology Pub Date : 2018-09-01 DOI: 10.5145/ACM.2018.21.3.64

H. Park, B. Son, Min Song, K. Shin

引用次数: 0

Clinical Utility of Fecal Immunochemical Transferrin Test in Gastrointestinal Bleeding Detection 粪便免疫化学转铁蛋白试验在胃肠道出血检测中的临床应用

Annals of Clinical Microbiology Pub Date : 2018-09-01 DOI: 10.5145/ACM.2018.21.3.51

Jongmin Lee, M. Park, W. Heo, K. Park, Y. Park, S. Han, Young-Seok Cho, Y. Park

{"title":"Clinical Utility of Fecal Immunochemical Transferrin Test in Gastrointestinal Bleeding Detection","authors":"Jongmin Lee, M. Park, W. Heo, K. Park, Y. Park, S. Han, Young-Seok Cho, Y. Park","doi":"10.5145/ACM.2018.21.3.51","DOIUrl":"https://doi.org/10.5145/ACM.2018.21.3.51","url":null,"abstract":"Background: Gastrointestinal (GI) bleeding can result from various conditions, including ulcers, neoplasms and infectious enterocolitis. The aim of this study was to evaluate the utility of the fecal immunochemical transferrin test compared with the fecal Hb test in various clinical settings. Methods: A total of 1,116 clinical stool specimens submitted for fecal occult blood testing were prospectively examined using both FIT Hb and FIT Tf kits (AlfresaPharma, Japan). To verify the specificity of the two tests, stool specimens from 265 health check-up examinees were also included. Results: A review of medical records revealed that 396 patients had clinical conditions associated with GI bleeding. FIT Hb and FIT Tf results were positive in 156 (39.4%) and 137 (34.6%) cases, respectively, and an additional 194 (49.0%) cases tested positive with either FIT Hb or FIT Tf. The two tests showed a moderate strength of agreement (kappa value; 0.56). Colitis (n=71) was associated with the most GI bleedings, followed by acute gastroenteritis (n=29), GI ulcers (n=27) and GI cancers (n=15). While the first two groups had higher positive rates on FIT Tf, patients in the latter two groups had higher positive rates on FIT Hb. Notably, four of nine specimens from premature babies tested positive only on FIT Tf. The specificity of FIT Hb and FIT Tf was 100% and 99.6%, respectively. Conclusion: Concurrent use of FIT Hb and FIT Tf improved the detection rate of occult GI bleeding, especially in patients with infectious GI disease (such as colitis or gastroenteritis) and in premature babies. (Ann Clin Microbiol 2018;21:51-57)","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":"21 1","pages":"51-57"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5145/ACM.2018.21.3.51","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44442598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Detection of Rifampicin Resistance in Mycobacterium tuberculosis by Using Middlebrook 7H9 Broth Medium with 2,3-Diphenyl-5-Thienyl-(2)-Tetrazolium Chloride 用含2,3-二苯基-5-噻吩基-（2）-四唑氯化铵的Middlebrook 7H9肉汤培养基检测结核分枝杆菌对利福平的耐药性

Annals of Clinical Microbiology Pub Date : 2018-09-01 DOI: 10.5145/ACM.2018.21.3.47

S. Lee, Kyung Jun Kim, Chulhun L. Chang

{"title":"Detection of Rifampicin Resistance in Mycobacterium tuberculosis by Using Middlebrook 7H9 Broth Medium with 2,3-Diphenyl-5-Thienyl-(2)-Tetrazolium Chloride","authors":"S. Lee, Kyung Jun Kim, Chulhun L. Chang","doi":"10.5145/ACM.2018.21.3.47","DOIUrl":"https://doi.org/10.5145/ACM.2018.21.3.47","url":null,"abstract":"Background: A simple and cost-effective method is needed for the detection of rifampicin resistance in Mycobacterium tuberculosis in resource-limited settings. We suggest a broth medium-based method using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride (STC) for detection of rifampin resistance of tubercle bacilli within a reasonable time frame. Methods: The type strain ( M. tuberculosis H37Rv) and 45 cultured clinical strains of M. tuberculosis (35 rifampin-susceptible and 10 rifampin-resistant) were used. Phenotypes of rifampicin resistance were tested by the Korea Institute of Tuberculosis, and con-firmed by GenoType MTBDRplus (Hain Lifescience, Germany). Susceptibility tests were performed using STC-containing OADC-enriched broth (BD, USA). Results: All tests were finished in 3 to 6 days. The same results were obtained with the standard and current methods for all 45 clinical isolates (100% sensitivity and specificity for resistance detection). Conclusion: The current method using STC is a good alternative for detecting M. tuberculosis rifampin resistance in a cost-effective and timely fashion, which is particularly important in resource-limited settings. (Ann Clin Microbiol 2018;21:47-50)","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":"21 1","pages":"47-50"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5145/ACM.2018.21.3.47","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45359122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0