Annals of Clinical Microbiology最新文献

Screening for potential infections in fecal microbiota transplantation guidelines and an experience of microbiota bank operation in Korea: a narrative review 粪便微生物群移植中潜在感染的筛查指南和韩国微生物群库的运作经验：叙述性综述

Annals of Clinical Microbiology Pub Date : 2024-03-20 DOI: 10.5145/acm.2024.27.1.3

Dongeun Yong

{"title":"Screening for potential infections in fecal microbiota transplantation guidelines and an experience of microbiota bank operation in Korea: a narrative review","authors":"Dongeun Yong","doi":"10.5145/acm.2024.27.1.3","DOIUrl":"https://doi.org/10.5145/acm.2024.27.1.3","url":null,"abstract":"Fecal microbiota transplantation (FMT) involves the transfer of fecal microbiota from healthy donors to patients to rectify dysbiosis and restore the functionality of the gut microbiota to a healthy state. Donor selection is important to minimize the risk of FMT. Donor selection for FMT is primarily focused on screening for potential infections. A complete consensus on screening tests and checkpoints is lacking and controversial; nevertheless, most guidelines agree to rule out certain infections, such as syphilis; hepatitis A, B, and C; and HIV. In most guidelines, stool testing includes testing for Clostridioides difficile and other enteric pathogens. The Korean FMT guidelines for C. difficile infections were published in 2022. The guidelines recommend serological and stool testing for donor candidates, with recommendations for stool testing providing targets for screening using specific test methods. Donor screening by Microbiotix Inc., a fecal microbiota bank in Korea, between 2017 and 2023 showed that only 5% of potential FMT donors were eligible for repeat donation. The future of FMT remains uncertain, with possibilities ranging from continuation to restrictions, and the development of synthetic microbiota preparations. Legislative support is crucial for advancing this field and providing hope and a potential cure for previously incurable patients.","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":" 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laboratory diagnosis of Clostridioides dif f icile infection: guidelines and status of practice in Korea 梭状芽孢杆菌感染的实验室诊断：韩国的指南和实践现状

Annals of Clinical Microbiology Pub Date : 2024-03-20 DOI: 10.5145/acm.2024.27.1.2

Hae-Sun Chung

{"title":"Laboratory diagnosis of Clostridioides dif f icile infection: guidelines and status of practice in Korea","authors":"Hae-Sun Chung","doi":"10.5145/acm.2024.27.1.2","DOIUrl":"https://doi.org/10.5145/acm.2024.27.1.2","url":null,"abstract":"Clostridioides difficile infection (CDI) is a common healthcare-associated infection that is expected to increase with the increases in the elderly population, immunocompromised patients treated with chemotherapy and immunosuppressive drugs, antimicrobial-resistant bacteria, and invasive medical technologies. Accurate diagnosis is critical for proper treatment and management of CDI. Clinical laboratories typically use four methods to diagnose CDI: C. difficile culture, toxin detection using immunoassays, detection of glutamate dehydrogenase using immunoassays, and detection of toxin A/B gene. Each CDI diagnostic test has strengths and limitations, and varies in performance. Guidelines for CDI diagnosis have been developed by organizations that include the European Society of Clinical Microbiology and Infectious Diseases, Infectious Diseases Society of America/Society for Healthcare Epidemiology of America, and American College of Gastroenterology. Certain other countries have guidelines. In Korea, surveys on CDI diagnosis performed in 2015 and 2018 revealed a shift in CDI testing in clinical laboratories in Korea. It is necessary to develop standardized diagnostic guidelines for CDI appropriate for the Korean context.","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":" 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ACM’s transition to an English journal: A leap towards international recognition ACM 向英文期刊过渡：迈向国际认可的飞跃

Annals of Clinical Microbiology Pub Date : 2024-03-20 DOI: 10.5145/acm.2024.27.1.1

Hae-Sun Chung

引用次数: 0

Emergence of Vanrija humicola as a pathogen of urinary tract infections in Korea Vanrija humicola 成为韩国尿路感染的病原体

Annals of Clinical Microbiology Pub Date : 2024-03-20 DOI: 10.5145/acm.2024.27.1.5

Jae Won Lee, Eun Jeong Won, H. Sung, Mi-Na Kim

引用次数: 0

Quantum dot-based lateral flow immunoassay for the rapid detection of pathogens 用于快速检测病原体的基于量子点的侧流免疫分析法

Annals of Clinical Microbiology Pub Date : 2023-12-20 DOI: 10.5145/acm.2023.26.4.99

Soo-Kyung Kim

引用次数: 0

Evaluation of the efficacy of three medical device detergents on bacteria and yeast derived biofilm: a comparative study 评估三种医疗器械清洁剂对细菌和酵母衍生生物膜的功效：一项比较研究

Annals of Clinical Microbiology Pub Date : 2023-12-20 DOI: 10.5145/acm.2023.26.4.117

Kuenyoul Park, Mi-Na Kim, H. Sung

{"title":"Evaluation of the efficacy of three medical device detergents on bacteria and yeast derived biofilm: a comparative study","authors":"Kuenyoul Park, Mi-Na Kim, H. Sung","doi":"10.5145/acm.2023.26.4.117","DOIUrl":"https://doi.org/10.5145/acm.2023.26.4.117","url":null,"abstract":"Background: This study aimed to evaluate the efficacy of three medical detergents against bacteria and yeast-derived biofilms. Methods: The biofilm removal efficacy of EmpowerTM (Metrex, USA), CidezymeTM (Johnson and Johnson Medical Inc, USA), and Matrix mintTM (Whiteley Medical, Australia) were compared to that of chlorine bleach. Biofilms were produced using Staphylococcus aureus RN9120, Escherichia coli ATCC35218, Pseudomonas aeruginosa ATCC27853, Candida albicans ATCC14053, and clinical isolates of Enterococcus faecalis, E. coli, Klebsiella pneumoniae, Candida auris, and Trichosporon asahii. The organisms were suspended in tryptic soy broth (TSB) in 96-well microplates and cultured for 72 hours. They were treated with the detergents, and the residual biofilm mass was quantified using crystal violet staining followed by optical density measurements at 620 nm (OD620 ). Results: EmpowerTM and CidezymeTM significantly reduced the biofilm mass derived from all species by > 50% of OD620 at 37ºC except those from E. faecalis, T. asahii, and C. auris. Matrix mintTM had no effect on the biofilms under any condition. Conclusion: The culture conditions and the species of the biofilm-producing organism influenced the effectiveness of the detergent. Biofilms produced by E. faecalis, C. auris, and T. asahii were resistant to all detergent treatments under all conditions.","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":"127 44","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138953557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance evaluation of 4-day versus 5-day blood cultures using the BD BACTEC FX system 使用 BD BACTEC FX 系统进行 4 天与 5 天血液培养的性能评估

Annals of Clinical Microbiology Pub Date : 2023-12-20 DOI: 10.5145/acm.2023.26.4.125

Jiyeon Kim, H. Sung, Mi-Na Kim

{"title":"Performance evaluation of 4-day versus 5-day blood cultures using the BD BACTEC FX system","authors":"Jiyeon Kim, H. Sung, Mi-Na Kim","doi":"10.5145/acm.2023.26.4.125","DOIUrl":"https://doi.org/10.5145/acm.2023.26.4.125","url":null,"abstract":"Background: Blood culture (BC) systems have evolved to increase sensitivity and reduce turnaround times. This study compared the performance of a 4-day versus a 5-day BC incubation period using the BD BACTEC™ FX (Becton, Dickinson and Company, USA). Methods: A total of 37,379 consecutive sets of BC were evaluated over a 3-month period in a 2,700-bed tertiary care hospital. Positive BC results were reviewed to assess time-to-positivity (TTP) and species identification of the isolates. The BCs were performed in pairs of vials, utilizing either BD BACTEC Plus Aerobic/F or Peds Plus/F with BD BACTEC Lytic Anaerobic media. Results: A total of 14,899 episodes, averaging 2.51 sets per episode, were analyzed. Of these, 1,398 (9.38%) were positive, yielding 1,465 isolates. TTP (hours) were < 12 in 48.87%, 12-24 in 31.40%, 24-48 in 13.38%, 48-72 in 3.28%, 72-96 in 1.43%, and >96 in 1.64%. The two most prevalent organisms, Escherichia coli and Klebsiella spp. were detected within 12 hours in 88.75% and 78.90%, respectively. The respective median TTP (T50) values for E. coli, Klebsiella spp., Enterococcus faecalis/E. faecium, and Staphylococcus aureus were 9.24, 9.60, 13.75, and 14.20. T50 values for these species were significantly shorter in anaerobic bottles than in aerobic bottles. Of 24 BCs with TTP > 96, only 4 containing anaerobic bacteria or molds were first detected after 96 hours. Conclusion: A 4-day incubation has demonstrated excellent sensitivity. However, a 5-day incubation may be beneficial for hospitals caring for patients at high risk for infections with slow-growing fungi or fastidious bacteria.","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":"14 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139168494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental culture for carbapenemase-producing Enterobacterales 产碳青霉烯酶肠杆菌的环境培养

Annals of Clinical Microbiology Pub Date : 2023-12-20 DOI: 10.5145/acm.2023.26.4.159

Hae-Sun Chung

引用次数: 0

Clinical manifestations, diagnosis, and antimicrobial resistance of group A streptococci infections A 组链球菌感染的临床表现、诊断和抗菌药耐药性

Annals of Clinical Microbiology Pub Date : 2023-12-20 DOI: 10.5145/acm.2023.26.4.89

Sunjoo Kim

引用次数: 0

Taenia saginata infection incidentally detected during workup for lymphoma from an 8-year-old boy in Korea: a case report 韩国一名 8 岁男孩在淋巴瘤检查过程中意外发现矢形梭菌感染：病例报告

Annals of Clinical Microbiology Pub Date : 2023-12-20 DOI: 10.5145/acm.2023.26.4.165

Eun Jeong Won, Min Jae Kim, Jina Lee, Hyery Kim, H. Sung, Mi-Na Kim

{"title":"Taenia saginata infection incidentally detected during workup for lymphoma from an 8-year-old boy in Korea: a case report","authors":"Eun Jeong Won, Min Jae Kim, Jina Lee, Hyery Kim, H. Sung, Mi-Na Kim","doi":"10.5145/acm.2023.26.4.165","DOIUrl":"https://doi.org/10.5145/acm.2023.26.4.165","url":null,"abstract":"Human taeniasis is presumed to have almost disappeared from Korea. Recently, we incidentally detected a Taenia saginata infection in an 8-year-old boy undergoing lymphoma diagnosis. The patient had been suffering for 4 months from intensifying snoring and obstructive sleep apnea. A neck computed tomography scan revealed a nasopharyngeal mass, and malignant B-cell lymphoma was supported by punch biopsy. On day 6 of the lymphoma workup period, the patient experienced anal itching, and two proglottids were detected in his stool. The patient had experienced four or five similar episodes within the past 2 years. He self-reported a history of raw beef and fish consumption and no history of traveling abroad. Laboratory findings revealed mild eosinophilia (eosinophil count: 791/μL). Two proglottids exhibited movement and possessed more than 15 branched uterine structures. Long segments approximately 84 cm in length were expelled after praziquantel treatment. Sequencing of the cytochrome oxidase 1 gene confirmed T. saginata, ruling out related Taenia species. After treatment, no proglottids or ova were detected in his stool, and the patient finally started chemotherapy for lymphoma. This case highlights the importance of timely diagnosis of hidden taeniasis in low-frequency endemic regions.","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":"129 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138953457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0