Annals of Clinical Microbiology最新文献_第7页

Evaluation of Synergistic Effect of Combined Treatment with Linalool and Colistin on Multidrug-Resistant Acinetobacter baumannii to Expand Candidate for Therapeutic Option 芳樟醇和粘菌素联合治疗多药耐药鲍曼不动杆菌的增效效果评价，扩大治疗选择

Annals of Clinical Microbiology Pub Date : 2020-03-01 DOI: 10.5145/acm.2020.23.1.11

Ung-Jun Kim, Choon-Mee Kim, S. Jang, Seul-Bi Lee, Seong-Sik Cho, Seokjin Jeong, Y. Ko, Seong-Ho Kang, Geon Park, Dong-Min Kim, Nara Yoon, Y. Ahn, Donghoon Lim, J. Kook

{"title":"Evaluation of Synergistic Effect of Combined Treatment with Linalool and Colistin on Multidrug-Resistant Acinetobacter baumannii to Expand Candidate for Therapeutic Option","authors":"Ung-Jun Kim, Choon-Mee Kim, S. Jang, Seul-Bi Lee, Seong-Sik Cho, Seokjin Jeong, Y. Ko, Seong-Ho Kang, Geon Park, Dong-Min Kim, Nara Yoon, Y. Ahn, Donghoon Lim, J. Kook","doi":"10.5145/acm.2020.23.1.11","DOIUrl":"https://doi.org/10.5145/acm.2020.23.1.11","url":null,"abstract":"Background: Acinetobacter baumannii infection is a significant health problem worldwide due to increased drug resistance. The limited antimicrobial A total of 51 strains of A. baumannii clinical isolates, consisting of 10 MDRAB and XDRAB were tested. We determined the minimum inhibitory concentration (MIC) of linalool for the test strains using the broth microdilution method and searched for interactions using the time-kill assay. time-kill assay linalool colistin rate of synergy synergy criteria 2), low rate of indifference (7.8%), a of bactericidal activity (74.5%) the 51 clinical isolates baumannii. The synergy rates for the linalool and colistin combination against MDRAB and XDRAB were 96% 92.1%, respectively. No antagonism was observed for the linalool and colistin combination. Conclusions: The combination of linalool and colistin showed a high synergy rate, which may be beneficial for controlling MDRAB infections. Therefore, this combination is a good candidate for in vivo studies to assess its efficacy in the treatment of MDRAB infections.","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46905028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Frequency of Mycobacterium tuberculosis Among M. tuberculosis Complex Strains Isolated from Clinical Specimen 临床分离结核分枝杆菌复合菌株中结核分枝杆菌的频率

Annals of Clinical Microbiology Pub Date : 2020-03-01 DOI: 10.5145/acm.2020.23.1.21

H. Cho, Jong-Bae Kim, Y. Uh

{"title":"Frequency of Mycobacterium tuberculosis Among M. tuberculosis Complex Strains Isolated from Clinical Specimen","authors":"H. Cho, Jong-Bae Kim, Y. Uh","doi":"10.5145/acm.2020.23.1.21","DOIUrl":"https://doi.org/10.5145/acm.2020.23.1.21","url":null,"abstract":"Background: Rapid and accurate detection of Mycobacterium tuberculosis (MTB) is of primary importance for infection control and selection of anti-tuberculosis drugs. However, most clinical laboratories report MTB complex (MTC) without reporting MTB because MTC comprising MTB, Mycobacterium bovis, Mycobacterium africanum, Mycobacterium microti, Mycobacterium caprae and Mycobacterium pinnipedii have 99.9% similarity at the nucleotide level and identical 16S rRNA sequences. This study was conducted to analyze the species frequency of MTC isolates obtained from clinical specimen. Methods: Of 310 MTC isolates obtained from clinical samples in a tertiary care hospital from February 2017 to August 2018, MolecuTech Real TB-Taq (YD Diagnostics, Korea) real-time PCR was performed, specifically to detect MTB. For DNA showing MTB negative results by MTBspecific real-time PCR or pyrazinamide-resistant strains, PCR-based MTC typing, spoligotyping, and exact tandem repeat D gene sequencing were performed. Results: All the 310 MTC isolates were identified to be MTB. Two MTB strains of East-AfricanIndian 4-Vietnam genotype, which have not been reported in Korea, were also found. Conclusion: There was no zoonotic tuberculosis in this study. Since we investigated only 310 MTC isolates detected in only one medical institution, multi-center study is needed to accurately know the prevalence of zoonotic tuberculosis in Korea.","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46831466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Application of 16S rRNA Gene-Targeted Next-Generation Sequencing for Bacterial Pathogen Detection in Continuous Ambulatory Peritoneal Dialysis Peritonitis 16S rRNA基因靶向下一代测序在连续性腹膜透析性腹膜炎病原菌检测中的应用

Annals of Clinical Microbiology Pub Date : 2020-03-01 DOI: 10.5145/acm.2020.23.1.1

Y. A. Kim, E. Kang, Hye Su Moon, Daewon Kim, D. Yong

{"title":"Application of 16S rRNA Gene-Targeted Next-Generation Sequencing for Bacterial Pathogen Detection in Continuous Ambulatory Peritoneal Dialysis Peritonitis","authors":"Y. A. Kim, E. Kang, Hye Su Moon, Daewon Kim, D. Yong","doi":"10.5145/acm.2020.23.1.1","DOIUrl":"https://doi.org/10.5145/acm.2020.23.1.1","url":null,"abstract":"Background: 16S rRNA gene-targeted next-generation sequencing (NGS) can detect microorganisms in a comprehensive reference database. To date, NGS has been successfully applied to samples such as urine, blood, and synovial fluid. However, there is no data for continuous ambulatory peritoneal dialysis (CAPD) fluid. The purpose of this study was to evaluate the clinical usefulness of microbiome analysis of CAPD fluids for the diagnosis of CAPD peritonitis. Methods: We included 21 patients with high suspicion of CAPD peritonitis. Routine CAPD fluid culture was performed using a pellet of 50 mL CAPD fluid onto the chocolate and blood agar for two days, and thioglycollate broth for one week. 16S rRNA gene-targeted NGS of pellets, stored at -70°C was performed with MiSeq (Illumina, USA). Results: Many colonized or pathogenic bacteria were detected from CAPD fluids using NGS and the microbiomes were composed of 1 to 29 genera with a cut-off 1.0. Compared to the culture results, NGS detected the same pathogens in 6 of 18 valid results (three samples failed with low read count). Additionally, using NGS, anaerobes such as Bacteroides spp. and Prevotella spp. were detected in six patients. In two of five samples in which no bacterial growth was detected, possible pathogens were detected by NGS. Conclusion: To our knowledge, this is the first report about the application of 16S rRNA genetargeted NGS for diagnosis of CAPD peritonitis. Etiology of culture-negative CAPD peritonitis can be better defined in NGS. Furthermore, it also helped the detection of anaerobic bacteria.","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5145/acm.2020.23.1.1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48394650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Season and Temperature Effects on Bloodstream Infection Incidence in a Korean Tertiary Referral Hospital 季节和温度对韩国三级转诊医院血流感染发生率的影响

Annals of Clinical Microbiology Pub Date : 2020-03-01 DOI: 10.5145/acm.2020.23.1.33

Y. Sohn, Jung-Hyun Byun, Y. A. Kim, Dong-Chun Shin, Kyungwon Lee

{"title":"Season and Temperature Effects on Bloodstream Infection Incidence in a Korean Tertiary Referral Hospital","authors":"Y. Sohn, Jung-Hyun Byun, Y. A. Kim, Dong-Chun Shin, Kyungwon Lee","doi":"10.5145/acm.2020.23.1.33","DOIUrl":"https://doi.org/10.5145/acm.2020.23.1.33","url":null,"abstract":"Background: The weather has well-documented effects on infectious disease and reports suggest that summer peaks in the incidences of gram-negative bacterial infections among hospitalized patients. We evaluated how season and temperature changes affect bloodstream infection (BSI) incidences of major pathogens to understand BSI trends with an emphasis on acquisition sites. Methods: Incidence rates of BSIs by Staphylococcus aureus, Enterococcus spp., Escherichia coli, Klebsiella pneumoniae, Acinetobacter spp., and Pseudomonas aeruginosa were retrospectively analyzed from blood cultures during 2008–2016 at a university hospital in Seoul, Korea according to the acquisition sites. Warm months (June–September) had an average temperature of ≥20 °C and cold months (December–February) had an average temperature of ≤5 °C. Results: We analyzed 18,047 cases, where 43% were with community-onset BSI. E. coli (N = 5,365) was the most common pathogen, followed by Enterococcus spp. (N = 3,980), S. aureus (N = 3,075), K. pneumoniae (N = 3,043), Acinetobacter spp. (N = 1,657), and P. aeruginosa (N = 927). The incidence of hospital-acquired BSI by Enterococcus spp. was weakly correlated with temperature, and the median incidence was higher during cold months. The incidence of community-onset BSI by E. coli was higher in warm months and was weakly correlated with temperature. Conclusion: We found seasonal or temperature-associated variation in some species-associated BSIs. This could be a useful information for enhancing infection control and public health policies by taking season or climate into consideration.","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48465911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Case Report of Saccharomyces cerevisiae Fungemia in a Premature Infant Following Probiotic Treatment 早产儿经益生菌治疗后出现酿酒酵母菌血症1例

Annals of Clinical Microbiology Pub Date : 2020-01-01 DOI: 10.5145/acm.2020.23.3.5

引用次数: 0

Evaluation of the AdvanSure TB/NTM Plus Real-Time PCR Assay for the Simultaneous Detection of Mycobacterium tuberculosis and Nontuberculous Mycobacteria from Clinical Specimens advanure TB/NTM + Real-Time PCR同时检测临床标本中结核分枝杆菌和非结核分枝杆菌的评价

Annals of Clinical Microbiology Pub Date : 2020-01-01 DOI: 10.5145/acm.2020.23.2.7

引用次数: 0

Epidemiological Study of an Outbreak of KPC-2-producing Klebsiella pneumoniae in a Tertiary Hospital in Korea 韩国某三级医院爆发产kpc -2肺炎克雷伯菌的流行病学研究

Annals of Clinical Microbiology Pub Date : 2020-01-01 DOI: 10.5145/acm.2020.23.2.5

J. Hong, Byeol Yi Park, Dokyun Kim, K. Kim, K. Lee, N. Cho, S. Jeong

{"title":"Epidemiological Study of an Outbreak of KPC-2-producing Klebsiella pneumoniae in a Tertiary Hospital in Korea","authors":"J. Hong, Byeol Yi Park, Dokyun Kim, K. Kim, K. Lee, N. Cho, S. Jeong","doi":"10.5145/acm.2020.23.2.5","DOIUrl":"https://doi.org/10.5145/acm.2020.23.2.5","url":null,"abstract":"Background: The prevalence of carbapenemase-producing Enterobacteriaceae (CPE), especially the KPC-2-producing Klebisella pneumoniae, is rapidly increasing and becoming a menace to global public health. This study aims to present the molecular epidemiology of the KPC-2-producing K. pneumoniae isolates emerged in a tertiary hospital in South Korea and describe its clinical significance. Methods: This study included carbapenem-resistant K. pneumoniae isolates collected from a tertiary hospital from April to December in 2018. Antimicrobial susceptibility of K. pneumoniae isolates was tested using disk diffusion method. PCR and DNA sequence analyses were performed to identify the resistance genotype. In addition, the molecular epidemiology was investigated using pulsed-field gel electrophoresis (PFGE) and multilocus sequencing typing (MLST). Results: Total 100 KPC-2-producing K. pneumoniae isolates were collected, which were mainly classified into two pulsotypes according to the XbaI restriction digestion pattern by PFGE analysis (pulsotype A, n = 31; pulsotype B, n = 63). The isolates exhibiting pulsotype A belonged to ST395 and the remaining isolates exhibiting pulsotype B were attributed to ST307 by MLST analysis. Conclusion: This study investigated clinical information and molecular bacterial profiles for KPC-2-producing K. pneumoniae isolates. These findings indicate that the proper infection control activities are needed to prevent the spread of multidrug-resistant organisms such as CPE, which could cause high mortality in clinical field.","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70969575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Types, Production and Validation of Reference Materials for Viral Genetic Testing 病毒基因检测标准物质的类型、生产和验证

Annals of Clinical Microbiology Pub Date : 2020-01-01 DOI: 10.5145/acm.2020.23.2.2

引用次数: 1

Evaluation of the Performance of ASTA MicroIDSys, a Novel Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry System, in Identification of Bacterial Clinical Isolates 新型基质辅助激光解吸/电离飞行时间质谱系统ASTA MicroIDSys在临床分离细菌鉴定中的性能评价

Annals of Clinical Microbiology Pub Date : 2020-01-01 DOI: 10.5145/acm.2020.23.3.3

Changseung Liu, E. J. Lee, Dokyun Kim, S. Jeong

{"title":"Evaluation of the Performance of ASTA MicroIDSys, a Novel Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry System, in Identification of Bacterial Clinical Isolates","authors":"Changseung Liu, E. J. Lee, Dokyun Kim, S. Jeong","doi":"10.5145/acm.2020.23.3.3","DOIUrl":"https://doi.org/10.5145/acm.2020.23.3.3","url":null,"abstract":"Background: We evaluated the performance of ASTA MicroIDSys (ASTA, Korea) and Bruker Biotyper (Bruker Daltonics, Germany) systems in the identification of bacterial isolates from clinical microbiology laboratory specimens during the study period. In addition, species for which the identification accuracy using MALDI-TOF MS systems was previously reported to be poor were also identified by comparing the MS results with those obtained using molecular identification. Methods: A total of 889 non-duplicated clinical isolates were included in this study. The results of ASTA MicroIDSys were compared with those of Bruker Biotyper; 16S rRNA sequencing was performed for the species for which results obtained using the two systems did not match. The sequences of rpoB, hisA, and/or recA for the clinical isolates of Acinetobacter species, Klebsiella species, and Burkholderia cepacia complex were analyzed and used as reference identifications. Results: The concordance rates for bacterial identification using ASTA MicroIDSys and Bruker Biotyper were 100% at the genus level and 98.3% at the species level for isolates belonging to the order Enterobacterales. Similarly, the concordance rates at the genus and species levels were 98.8% and 91.0% for glucose non-fermenting bacilli, 100% and 100% for gram-positive cocci, and 98.9% and 98.9% for other isolates, respectively. ASTA MicroIDSys was expected to correctly identify 97.9% of the 108,251 isolates identified in our clinical microbiology laboratory over the past 5 years. Conclusion: ASTA MicroIDSys showed excellent performance in bacterial identification for most of the clinically relevant species. Further extension of the database could improve the identification accuracy of ASTA MicroIDSys.","PeriodicalId":34065,"journal":{"name":"Annals of Clinical Microbiology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70970358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Isolation of Carnobacterium divergens from Blood Culture in Korea : A Case Report and Literature Review 韩国血液培养分离分化肉杆菌1例报告及文献复习

Annals of Clinical Microbiology Pub Date : 2020-01-01 DOI: 10.5145/acm.2020.23.3.4

引用次数: 2