Clocks & Sleep最新文献

{"title":"Cross-Sectional Analysis of Sleep Quality and Vascular Health in Shift- and Day-Working Nurses.","authors":"Gleb Saharov, Barbara Salti, Maram Bareya, Anat Keren-Politansky, Yona Nadir, Tamar Shochat","doi":"10.3390/clockssleep7040058","DOIUrl":"10.3390/clockssleep7040058","url":null,"abstract":"<p><p>Sleep disturbances and shift work are associated with increased cardiovascular risk, possibly through disruptions in endothelial and hemostatic function. While prior studies link acute sleep deprivation to vascular dysfunction, the impact of chronic sleep quality and circadian misalignment on endothelial health in healthy individuals, particularly shift workers, remains underexplored. The aim of this study was to examine the association between objectively measured sleep quality and endothelial/hemostatic function in healthy female hospital nurses, comparing shift and day workers, and considering time-of-day variation. In this repeated-measures study, 100 female nurses (51 shift, 49 day workers) aged 25-50 wore actigraphy devices for 7-14 days to assess total sleep time (TST), sleep efficiency (SEF), and wake after sleep onset (WASO). Endothelial function was measured using EndoPAT (Reactive Hyperemia Index-RHI). Hemostatic markers included plasminogen activator inhibitor-1 (PAI-1), von Willebrand factor (VWF), heparanase and heparanase procoagulant activity assessed by ELISA, and chromogenic assays in morning and evening. TST was not associated with any vascular outcomes. Poor sleep quality (low SEF, high WASO) was significantly associated with reduced RHI and elevated PAI-1 level, heparanase level, and heparanase procoagulant activity levels. Regression models revealed significant main effects of SEF and WASO on endothelial and coagulation markers, with some interactions depending on shift type and time of measurement. No significant associations were found for VWF. Impaired sleep quality, but not sleep duration, is associated with endothelial dysfunction and procoagulant activation, particularly among shift-working nurses. These findings suggest that sleep quality may play a critical role in vascular health and support the use of sleep-based interventions to reduce cardiovascular risk in shift-working populations.</p>","PeriodicalId":33568,"journal":{"name":"Clocks & Sleep","volume":"7 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12551026/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145356242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does Daytime Sleepiness Moderate the Relationship Between Working Memory and Academic Performance in Schoolchildren? A Pilot Study.","authors":"Sergey Malykh, Valeriia Demareva","doi":"10.3390/clockssleep7040057","DOIUrl":"10.3390/clockssleep7040057","url":null,"abstract":"<p><p>Academic performance in adolescence is influenced by both cognitive capacity and physiological factors such as sleepiness. However, the interaction between these dimensions remains understudied. This pilot study examined whether daytime sleepiness moderates the relationship between working memory and academic achievement in a sample of 601 schoolchildren aged 11 to 17 years. Participants completed a digital visuospatial working memory task and self-reported their daytime sleepiness using the Pediatric Daytime Sleepiness Scale (PDSS). Academic performance was assessed through official grades in Mathematics, Language, and Literature. Regression analyses showed that working memory (total score and average reaction time) and daytime sleepiness were independent predictors of academic performance. These findings support our hypotheses that cognitive and physiological factors each contribute to school success. However, no significant moderation effects were found in the full sample. Subgroup analyses revealed that working memory predicted academic outcomes only among students with normal sleepiness levels, whereas in high-sleepiness students, cognitive predictors lost significance and PDSS scores emerged as the dominant predictor. These results suggest that elevated daytime sleepiness can undermine the positive impact of working memory on academic performance. The findings highlight the importance of assessing both cognitive skills and physiological readiness when evaluating students. They also suggest that sleep-focused interventions may improve learning outcomes, especially during adolescence.</p>","PeriodicalId":33568,"journal":{"name":"Clocks & Sleep","volume":"7 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12550925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145356290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-Reported Insomnia and Poor Sleep Quality Are Associated with Self-Reported Cognitive Changes in Older Adults.","authors":"Julia Glueck, Celina Pluim McDowell, Yakeel T Quiroz, Alice Cronin-Golomb, Jeanne F Duffy","doi":"10.3390/clockssleep7040056","DOIUrl":"10.3390/clockssleep7040056","url":null,"abstract":"<p><p>Older adults are vulnerable to changes in sleep with age. Poor sleep quality is associated with self-reported cognitive changes, which can occur before the onset of objective cognitive decline associated with Mild Cognitive Impairment and Alzheimer's disease. The objective of this study was to examine associations between self-reported sleep complaints, objective sleep quality, and self-reported cognitive changes and their relations to symptoms of depression and anxiety in a group of community-dwelling older adults. Adults aged ≥ 50 without dementia (<i>n</i> = 45) were recruited and completed 1-2 weeks of rest-activity monitoring using a wrist-worn device, underwent a test of global cognitive functioning (Mini-Mental State Examination; MMSE), and completed questionnaires assessing insomnia (Insomnia Severity Index; ISI), subjective sleep quality (Pittsburgh Sleep Quality Index; PSQI), self-reported cognitive changes (Cognitive Function Instrument; CFI), and symptoms of depression and anxiety (Beck Depression Inventory-II; BDI-II and Generalized Anxiety Disorder 7-item assessment; GAD-7). Pearson partial correlations assessed relations among subjective and objective sleep quality, insomnia ratings, CFI ratings, and global cognition, while controlling for BDI-II and GAD-7 ratings. Exploratory analyses examined the correlation between PSQI component scores and CFI ratings and global cognition. Greater ISI (<i>r</i> = 0.50, <i>p</i> ≤ 0.001) ratings significantly correlated with higher CFI scores. PSQI total ratings and actigraphy-based measures (<i>n</i> = 41) did not significantly correlate with CFI scores. Exploratory PSQI subscale analyses revealed that worse subjective sleep quality (<i>r</i> = 0.31, <i>p</i> = 0.048), shorter sleep duration (<i>r</i> = 0.32, <i>p</i> = 0.04), and greater use of sleep medications (<i>r</i> = 0.31, <i>p</i> = 0.048) correlated with higher CFI scores. Poorer sleep quality due to less time spent asleep, fragmented or disturbed sleep, and requiring medications to sleep, may be associated with greater memory concerns. Alternatively, worries about cognition may deleteriously affect sleep. Subjective measures of sleep quality may be useful to identify older adults at increased risk of cognitive decline.</p>","PeriodicalId":33568,"journal":{"name":"Clocks & Sleep","volume":"7 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12551010/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145356267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Under the Covers: The Effect of a Temperature-Controlled Mattress Cover on Sleep and Perceptual Measures in Healthy Adults.","authors":"Shauna Stevenson, Haresh Suppiah, Toby Mündel, Matthew Driller","doi":"10.3390/clockssleep7040055","DOIUrl":"10.3390/clockssleep7040055","url":null,"abstract":"<p><p>Ambient temperature and thermoregulation influence sleep quality. This study investigated the effects of a temperature-controlled mattress cover on sleep and perceptual outcomes in healthy adults. In a randomised, counterbalanced, crossover design, 34 healthy adults (20 F, 14 M; age, 30 ± 5 y) used a temperature-controlled mattress cover for 14 nights, following ≥3 nights of familiarisation. The temperature feature was on for 7 nights (POD) and off for 7 nights (CON). Sleep was assessed via wrist actigraphy, while heart rate (HR), heart rate variability (HRV), and respiratory rate (RR) were recorded by embedded sensors in the mattress cover. Participants completed daily and weekly questionnaires evaluating sleep quality, thermal comfort, and thermal sensation. Linear mixed models showed significant main effects of condition favouring POD over CON for all daily perceived outcomes (all <i>p</i> < 0.05). A large, significant improvement in perceived sleep quality was observed (<i>p</i> = 0.001, d = 0.92). No significant differences were found in objective sleep metrics or biometric measures (all <i>p</i> ≥ 0.05). A temperature-controlled mattress cover was associated with improved subjective sleep quality and thermal-related perceptions despite minimal changes in objective or biometric outcomes, which may in part reflect expectancy, or placebo effects. Further research is needed to explore whether these perceptual benefits lead to physiological improvements over time.</p>","PeriodicalId":33568,"journal":{"name":"Clocks & Sleep","volume":"7 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12550930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145356233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reporter-Mediated Evaluation of the Circadian Oscillations of <i>SNAIL</i> Across In Vitro Models.","authors":"Kaitlyn Chhe, Bhavna Kalyanaraman, Sophie A Spielberger, Hui-Hsien Lin, Stephanie R Taylor, Michelle E Farkas","doi":"10.3390/clockssleep7040054","DOIUrl":"10.3390/clockssleep7040054","url":null,"abstract":"<p><p>The protein SNAIL has been widely studied for its roles in promoting cancer invasion and resistance to apoptosis. There are multiple contributors to its expression, including self- and circadian regulation, and it has been posited that <i>SNAIL</i> oscillates in a circadian manner. Given the multiple factors involved, we sought to determine whether this is indeed the case. We developed a luciferase reporter that was used to demonstrate <i>SNAIL</i>'s rhythmic nature (<i>SNAIL:luc</i>) in the circadian model cell line, U2OS. Considering <i>SNAIL</i>'s relevance in breast cancer, we also assessed its oscillations in cellular models representing different levels of aggression. We incorporated the <i>SNAIL:luc</i> reporter in MCF10A breast epithelial cells, and MCF7 and MDA-MB-231 breast cancer cell lines, which are less and more aggressive, respectively. We found that <i>SNAIL</i> oscillations were present but weak in MCF7 and arrhythmic in MDA-MB-231 cells, correlating with those of core clock genes (<i>BMAL1</i> and <i>PER2</i>) in these models. Surprisingly, MCF10A cells, whose core clock genes possess robust circadian expression patterns, did not have rhythmic oscillations of <i>SNAIL</i>. Our findings suggest that <i>SNAIL</i> is under circadian control, but this is cell line/tissue dependent, setting the stage for additional studies to better understand the impacts of various factors contributing to its expression.</p>","PeriodicalId":33568,"journal":{"name":"Clocks & Sleep","volume":"7 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12550899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145356280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Crosstalk Between the Anterior Hypothalamus and the Locus Coeruleus During Wakefulness Is Associated with Low-Frequency Oscillations Power During Sleep.","authors":"Nasrin Mortazavi, Puneet Talwar, Ekaterina Koshmanova, Roya Sharifpour, Elise Beckers, Ilenia Paparella, Fermin Balda, Christine Bastin, Fabienne Collette, Laurent Lamalle, Christophe Phillips, Mikhail Zubkov, Gilles Vandewalle","doi":"10.3390/clockssleep7040053","DOIUrl":"10.3390/clockssleep7040053","url":null,"abstract":"<p><p>Animal studies show that sleep regulation depends on subcortical networks, but whether the connectivity between subcortical areas contributes to human sleep variability remains unclear. We investigated whether the effective connectivity between the LC and hypothalamic subparts during wakefulness relates to sleep electrophysiology. Thirty-three younger (~22 y, 27 women) and 18 late middle-aged (~61 y, 14 women) healthy individuals underwent 7-Tesla functional MRI during wakefulness to assess LC-hypothalamus effective connectivity. Additionally, sleep EEG was recorded at night in the lab to examine the relationships between effective connectivity measures and REM sleep theta energy as well as sigma power prior to REM. Connectivity analyses revealed strong mutual positive influences between the LC and both the anterior-superior and posterior hypothalamus, consistent with animal studies. Aging was negatively associated with the connectivity from the anterior-superior hypothalamus (including the preoptic area) to the LC. In late middle-aged adults, but not younger adults, stronger effective connectivity from the anterior-superior hypothalamus to the LC was associated with lower REM theta energy. This association extended to other low-frequency bands during REM and NREM sleep. These findings highlight the age-dependent modulation of LC-hypothalamus interactions and their potential roles in sleep regulation, providing new insights into neural mechanisms underlying age-related sleep changes.</p>","PeriodicalId":33568,"journal":{"name":"Clocks & Sleep","volume":"7 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12551093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145356245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Cross-Sectional Association Between Hypothyroidism and Circadian Syndrome: Insights from NHANES 2007-2012.","authors":"Ahmed Arabi, Humam Emad Rajha, Osama Alkeilani, Ahmad Hamdan, Dima Nasrallah, Giridhara R Babu","doi":"10.3390/clockssleep7040052","DOIUrl":"10.3390/clockssleep7040052","url":null,"abstract":"<p><strong>Background: </strong>Circadian Syndrome (CircS) encompasses a range of cardiometabolic risk factors that contribute to an increased susceptibility to cardiovascular diseases and type 2 diabetes. Understanding the factors that underpin CircS is essential. This study primarily aims to examine the association between hypothyroidism and CircS in adults. A secondary analysis compares this association with that between hypothyroidism and Metabolic Syndrome (MetS). Additionally, the dose-response relationship between serum free thyroxine (FT4) levels and CircS probability is explored.</p><p><strong>Methods: </strong>This cross-sectional study includes 4050 National Health and Nutrition Examination Survey (NHANES) participants (2007-2012). Hypothyroidism was classified into (1) drug-managed, (2) non-drug-managed (NDM) primary, and (3) NDM central hypothyroidism, based on self-reported medication use and serum TSH/FT4 levels. CircS was defined as having ≥5 of its eight components, including MetS criteria, depression, short sleep, and non-alcoholic fatty liver disease.</p><p><strong>Results: </strong>Our results showed that hypothyroidism was significantly associated with CircS (OR: 1.58, 95% CI 1.26-1.98) and MetS (OR: 1.19, 95% CI 1.01-1.42). An inverse, non-linear relationship between serum FT4 levels and the probability of CircS was observed.</p><p><strong>Conclusions: </strong>The results underscore a significant association between hypothyroidism and CircS and MetS, with FT4 levels inversely related to CircS probability. These findings highlight hypothyroidism's potential role in CircS pathogenesis and prevention.</p>","PeriodicalId":33568,"journal":{"name":"Clocks & Sleep","volume":"7 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12550944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145356293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sleepiness and Fatigue as Consequences of Cumulative Sleep Restriction: Insights from Fine-Grained Subjective Measures and Skin Temperature in the Field.","authors":"Vaida T R Verhoef, Karin C H J Smolders, Geert Peeters, Sebastiaan Overeem, Yvonne A W de Kort","doi":"10.3390/clockssleep7030051","DOIUrl":"10.3390/clockssleep7030051","url":null,"abstract":"<p><p>Diagnosis and monitoring of daytime sleepiness remain challenging and are strongly reliant on subjective assessments. To revisit common monitoring tools and explore new assessment modalities, we investigated the response of daily and momentary subjective measures of sleepiness and fatigue and skin temperature to sleep restriction, assessed between- and within-day variations in these responses, and studied their convergence. Seventeen healthy participants (aged 19-32 years, seven females, ten males) participated in a field study employing ecological momentary assessment. After a one-week baseline, two sleep conditions (4 h/night vs. 7-9 h/night, actigraphy-controlled; three nights each) were counterbalanced across participants. During the experimental conditions, sleepiness and fatigue were assessed using subjective rating scales administered in daily diary questionnaires and experience sampling questionnaires (10 notifications per day), while distal and proximal skin temperatures were continuously recorded. Results revealed significant effects of sleep restriction on distal and proximal skin temperature and daily reports of sleepiness and fatigue, independent of the number of sleep-restricted nights. The effects on momentary assessments were moderated by day, reflecting a cumulative effect of the sleep restriction from Days 1 to 3. The effects of sleep restriction on momentary sleepiness and fatigue ratings or hourly skin temperature metrics were not significantly moderated by time of day. Hourly skin temperatures (distal skin temperature and temperature gradient) were significantly related to subjective sleepiness and fatigue. In conclusion, all self-reports were sensitive to the sleep restriction, but momentary assessments illustrate the manipulation's cumulative effects and captured temporal dynamics in sleepiness and fatigue within days. This investigation showed overlap between sleepiness and fatigue experiences as reflected in medium to strong associations. Skin temperature correlates with momentary subjective sleepiness (and fatigue); however, considering it a proxy for daytime sleepiness remains exploratory.</p>","PeriodicalId":33568,"journal":{"name":"Clocks & Sleep","volume":"7 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452710/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Clinical Impact of Restless Legs Syndrome in Pediatric Populations with Attention-Deficit/Hyperactivity Disorder: A Systematic Review.","authors":"Toni Ghayad, Anaïs Mungo, Matthieu Hein","doi":"10.3390/clockssleep7030050","DOIUrl":"10.3390/clockssleep7030050","url":null,"abstract":"<p><p>Attention Deficit Hyperactivity Disorder (ADHD) is a prevalent disorder in the pediatric population. Furthermore, there appears to be a special relationship between ADHD and Restless Legs Syndrome (RLS). The objective of this review was therefore to provide an updated overview of the current literature regarding the prevalence of RLS and its potential clinical impact in pediatric ADHD subjects (<18 years). A systematic literature review was carried out in May 2025 in the PubMed-Medline database according to PRISMA criteria. After evaluation by two readers of the 147 identified articles, 9 articles investigating the prevalence of RLS with or without assessment of its potential clinical impact were selected for this systematic literature review. The prevalence of RLS in children and adolescents with ADHD showed significant variation, ranging from 11% to 54%. One study found a significant impact of RLS on academic performance and life skills in pediatric ADHD subjects. Three studies highlighted higher severity of ADHD complaints in subjects with comorbid RLS. One study reported higher RLS severity scores in the ADHD+RLS group and significantly more severe scores in the \"hyperactive-impulsive\" ADHD subtype. Two studies identified a significant association between a family history of RLS and RLS+ADHD comorbidity. Compared with the general pediatric population, the prevalence of RLS appears to be higher in pediatric ADHD subjects. Finally, this comorbid sleep disorder could worsen the severity of ADHD symptoms and complicate its clinical management.</p>","PeriodicalId":33568,"journal":{"name":"Clocks & Sleep","volume":"7 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sleep Quality Assessment in Intensive Care Units: Comparing Actigraphy and the Richards Campbell Sleep Questionnaire-A Pilot Study in the Moroccan Context.","authors":"Abdelmajid Lkoul, Keltouma Oumbarek, Youssef Bouchriti, Asmaa Jniene, Tarek Dendane","doi":"10.3390/clockssleep7030049","DOIUrl":"10.3390/clockssleep7030049","url":null,"abstract":"<p><p>Sleep in intensive care unit (ICU) patients is frequently disrupted, which may adversely affect their overall health and recovery. Despite the implementation of various strategies to promote sleep, accurately assessing its quality remains complex. This pilot study aimed to evaluate both the quality and quantity of sleep in ICU patients using actigraphy (ACT) and the Richards-Campbell Sleep Questionnaire (RCSQ) and to compare the diagnostic performance of these two tools. We conducted a prospective observational study including 228 ICU patients. Sleep was assessed using both RCSQ and ACT. Receiver Operating Characteristic (ROC) curve analysis was used to evaluate the discriminative ability of each tool (Area Under the Curve [AUC], sensitivity, specificity), with optimal cut-off points determined using Youden's Index. The Mann-Whitney U test was used to compare sleep parameters between patients classified as having good or poor sleep based on ACT measurements. The mean RCSQ score was 38.16 ± 17.09, indicating poor perceived sleep quality. Sleep onset latency (based on RCSQ) was 35.71 ± 21.44 min, with a mean of 40.32 ± 20.03 awakenings. According to ACT, sleep latency was 39.23 ± 22.09 min, and total sleep duration was 198.15 ± 128.42 min (approximately 3 h and 18 min), which is significantly below recommended levels. The average number of awakenings recorded was 24.85. In terms of diagnostic performance, the RCSQ demonstrated excellent discriminative ability (AUC = 1.00 for the total score), while ACT showed more variable results: total sleep duration had a good AUC of 0.91, while sleep latency showed a lower performance with an AUC of 0.50. The RCSQ proved to be more reliable than ACT in assessing sleep quality in ICU patients, providing consistent results across multiple parameters, including sleep depth, latency, and number of awakenings. Conversely, ACT yielded less consistent findings, particularly regarding sleep latency and nighttime interruptions. Further studies are warranted to refine objective tools for evaluating sleep in critically ill patients.</p>","PeriodicalId":33568,"journal":{"name":"Clocks & Sleep","volume":"7 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}