{"title":"Physiological effects of polycyclic aromatic hydrocarbons (PAH) in fish organism","authors":"T. S. Filatova, D. Abramochkin","doi":"10.55959/msu0137-0952-16-78-3-2","DOIUrl":"https://doi.org/10.55959/msu0137-0952-16-78-3-2","url":null,"abstract":"The pollution of world ocean is a serious threat to the biodiversity of flora and fauna. One of the most important sources of pollution are oil and oil products – every year more than 1 million tons of oil is spilled into the sea as a result of accidents during oil production or transportation. The teratogenic and carcinogenic effects of such oil components as benzopyrene is well known since the middle of the last century. However, after a large oil spill from “Exxon Valdez” tanker in 1989 it became clear that oil and its components have strong toxic effects in fish organism – and to a large extent these effects are caused by di- and tricyclic aromatic hydrocarbons, in particular phenanthrene. Field research have demonstrated that fish embryo and larvae are the most prone to the effects of oil – and the largest oil spills endanger the populations of important commercial fish species that spawn in this area. This review considers the influence of polycyclic aromatic hydrocarbons (PAH) on the physiology of various organ systems in fish. Particular attention is paid to the cardiotoxic effects of PAH which were recently discovered and which are potentially not only the main cause of animals’ death upon the spill of PAH into water, but also underlie the malformation of other organs.","PeriodicalId":334823,"journal":{"name":"Vestnik Moskovskogo universiteta. Seria 16. Biologia","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139252653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Todorenko, N.D. Sidochenko, A. Baizhumanov, L. B. Bratkovskaya, D. Matorin
{"title":"Primary photosynthetic processes of Thalassiosira weissflogii under the effect of ciprofloxacin","authors":"D. Todorenko, N.D. Sidochenko, A. Baizhumanov, L. B. Bratkovskaya, D. Matorin","doi":"10.55959/msu0137-0952-16-78-3-6","DOIUrl":"https://doi.org/10.55959/msu0137-0952-16-78-3-6","url":null,"abstract":"Fluoroquinolone antibiotics such as ciprofloxacin have been actively used in medical practice, including the COVID-19 pandemic, to suppress adverse bacterial infections. Widespread application and improper disposal have resulted in the ubiquity of antibiotics in the environment, which can affect aquatic life, including phytoplankton. The effect of fluoroquinolone antibiotics on the photosynthetic processes of marine diatoms, which are the main producers in marine ecosystems, has been little studied. In this work the effect of the antibiotic ciprofloxacin on the primary photosynthetic processes in the marine diatom Thalassiosira weissflogii was studied. It has been shown that ciprofloxacin affects the functioning of PSII, preventing the transfer of absorbed energy from the excited antenna chlorophyll molecules to the PSII RC (φDo). Under the influence of ciprofloxacin, a decrease in the efficiency of electron donation to P680+ (FV / FO), inhibition of the quantum yield of PSII (FV / FM), a decrease in the proportion of active RCs (ABS / RC), and an increase in the dissipation of absorbed energy in RCs (DIo / RC) were revealed. It has been shown that the mechanism of action of ciprofloxacin is associated with damage of PSII RC. Ciprofloxacin enhances the photosensitivity of microalgae and causes an increase in lipid peroxidation products. It is proposed to apply the parameters of chlorophyll fluorescence analyzing the effect of antibiotics on microalgae.","PeriodicalId":334823,"journal":{"name":"Vestnik Moskovskogo universiteta. Seria 16. Biologia","volume":" 40","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139254661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of the primary reactions of photosynthesis in microalgae single cell by microfluorimetric method","authors":"А.А. Volgusheva, I.V. Konyukhov, T. K. Antal","doi":"10.55959/msu0137-0952-16-78-3-4","DOIUrl":"https://doi.org/10.55959/msu0137-0952-16-78-3-4","url":null,"abstract":"High-resolution chlorophyll fluorescence light induction curves (OJIP transients) are widely used to assess the primary photosynthetic responses of phototrophic microorganisms. Chlorophyll fluorescence measuring methods coupled with microscopy techniques provide a promising opportunity to measure OJIP transients on individual algal cells, allowing scientists to investigate stress adaptation mechanisms related to reorganization of microalgae population or phytoplankton community. In this work, we first characterized the OJIP transients measured on individual algae cells using the original microfluorimeter and compared them with OJIP transients recorded in microalgae suspensions. Based on the results of the study, we proposed a method for analyzing OJIP curves of individual microalgae cells as well as ways to further improve microfluorimeters.","PeriodicalId":334823,"journal":{"name":"Vestnik Moskovskogo universiteta. Seria 16. Biologia","volume":"221 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139250921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. N. Pavlyuchenkova, I. Kutyrev, A. Fedorov, M. Chelombitko, O. E. Mazur, Z. Dugarov
{"title":"Investigation of excretory/secretory products from gull-tapeworm Dibothriocephalus dendriticus and ligula Ligula interrupta plerocercoids anti-inflammatory properties","authors":"A. N. Pavlyuchenkova, I. Kutyrev, A. Fedorov, M. Chelombitko, O. E. Mazur, Z. Dugarov","doi":"10.55959/10.55959/msu0137-0952-16-78-3-1","DOIUrl":"https://doi.org/10.55959/10.55959/msu0137-0952-16-78-3-1","url":null,"abstract":"In this work, the anti-inflammatory potential of secretory-excretory products (SEP) of gull- tapeworm Dibothriocephalus dendriticus and ligula Ligula interrupta plerocercoids was studied for the first time in an in vitro model of LPS-induced activation of macrophages. A monocyte cell line derived from a patient with acute monocytic leukemia, THP-1, was used as a macrophage model. The anti-inflammatory properties of SEP were determined by the content of tumor necrosis factor (TNF) and interleukin-6 cytokines in the incubation medium using commercial kits for enzyme immunoassay. The results of our study indicated that SEP from L. interrupta plerocercoids have a pronounced anti-inflammatory effect, while SEP from D. dendriticus plerocercoids did not have such an effect. Next, we investigated the anti- inflammatory properties of L. interrupta SEP in a carrageenan-induced air-sac inflammation model in mice. A significant decrease in the volume of inflammatory exudate under the influence of L. interrupta SEP was found, as well as an increase in the level of the interleukin-6 cytokine. At the same time, SEP of L. interrupta had no effect on the number of cells per 1 ml of exudate, as well as on the level of the pro-inflammatory cytokine TNF. The low molecular weight fraction of L. interrupta SEP also increased the level of the anti-inflammatory cytokine interleukin-10, which indicates a more pronounced anti-inflammatory effect compared to the high molecular weight fraction. The results obtained, in general, indicate the anti- inflammatory properties of the SEP of L. interrupta plerocercoids. However, the mechanism of anti-inflammatory action has not been elucidated and requires further research.","PeriodicalId":334823,"journal":{"name":"Vestnik Moskovskogo universiteta. Seria 16. Biologia","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139251385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Features of the reproductive biology of representatives of the genus Haslea Simonsen (Bacillariophyta), allowing the use of classical breeding methods","authors":"E.S. Kirienko, N. Davidovich","doi":"10.55959/msu0137-0952-16-78-3-9","DOIUrl":"https://doi.org/10.55959/msu0137-0952-16-78-3-9","url":null,"abstract":"The application of the classical selection of diatoms on the example of the genus Haslea is considered. The ability of some representatives of this genus to synthesize marennine and marennine-like pigments is interesting to researchers and owners of oyster farms because these unique pigments can colorise the tissues of mollusks in a greenish color and improve their organoleptic properties. It has been shown that marennine has antibacterial, antiviral, antioxidant and other biological activities. It is assumed that the efficiency of marennine biosynthesis can be increased by obtaining highly productive strains by selection, which was not previously carried out in diatoms. The assessment of the possibility and prospects of applying the methods of classical breeding in relation to representatives of the genus Haslea is given. It is shown that significant success has been achieved to date in the study of the life cycle, crossing systems and other features of the reproductive biology of representatives of the genus Haslea, which allows them to carry out classical selection by selection, hybridization and obtaining descendants of the next generation.","PeriodicalId":334823,"journal":{"name":"Vestnik Moskovskogo universiteta. Seria 16. Biologia","volume":"49 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139250900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bacteria of the genus Bacillus on the Russian segment of the International Space Station","authors":"R. R. Yenikeyev, L. M. Zakharchuk","doi":"10.55959/msu0137-0952-16-78-3-5","DOIUrl":"https://doi.org/10.55959/msu0137-0952-16-78-3-5","url":null,"abstract":"Pure cultures of 19 strains of spore-forming bacteria were obtained from the equipment surfaces of the Russian segment of the International Space Station. The study of morphological, cultural and physiological-biochemical properties of these bacteria allowed us to attribute all strains to the genus Bacillus. As a result of using MALDI-TOF methods and genome-wide sequencing, it was found that out of 19 bacillus strains, six belong to the species B. paralicheniformis, four to B. pumilus, four to B. subtilis, two to B. cereus and one to B. amyloliquefaciens. In accordance with the requirements and norms of EUCAST 2023, the resistance of bacillus strains obtained from the Russian segment of the International Space Station to antibiotics such as imipenem, meropenem, ciprofloxacin, levofloxacin, norfloxacin, vancomycin, erythromycin, clindamycin and linezolid was studied. Resistance to erythromycin was found in 11 strains of bacilli and five strains showed resistance to clindamycin. Only one strain showed resistance to imipenem, levofloxacin and norfloxacin, respectively. Analysis of the complete genome of bacterial strains in which resistance to erythromycin and (or) clindamycin was found made it possible to establish that resistance to these antibiotics in B. paralicheniformis strains SE71, SE131, SE181, SE182, SE183 provides the ermD antibiotic resistance gene. In B. cereus SE43, resistance to erythromycin encodes the mphL gene.","PeriodicalId":334823,"journal":{"name":"Vestnik Moskovskogo universiteta. Seria 16. Biologia","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139253279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Lavrenov, T. A. Myshliavkina, N. V. Umnova, A. Kim, V. Roumak
{"title":"PECULIARITY OF MARKER GENES’ EXPRESSION IN BANK VOLES CLETHRIONOMYS GLAREOLUS CHARACTERIZING ECOTOXICITY EFFECTS OF THE TERRITORY CONTAMINATED WITH DIOXINS","authors":"A. Lavrenov, T. A. Myshliavkina, N. V. Umnova, A. Kim, V. Roumak","doi":"10.55959/msu0137-0952-16-78-2-6","DOIUrl":"https://doi.org/10.55959/msu0137-0952-16-78-2-6","url":null,"abstract":"To assess the ecotoxicity of low doses of dioxins is almost impossible without considering the in uence of real exposure conditions on these substances’ properties. The best approach to take these into account is the biomonitoring of the initial toxic e ects’ manifestation. We studied bank voles from population naturally exposed to dioxins, the summer-born adults and overwintered functional groups of animals di ered by dioxin body burden. Dioxin-free samples of a vivarium bank voles’ line served as a control. Initial e ects of ecotoxicity were characterized by transcriptional levels of genetic markers: ahr, cyp1a2, keap1, dnmt1, dnmt3a, dnmt3b, LINE-1 and B1-SINE. Summer-born functional group had signi cantly higher expression levels of ahr, keap1, dnmt3a and dnmt3b genes versus their control group. Overwintered functional group had elevated expression levels of cyp1a2 and keap1, but no changes were found versus controls for dnmt1, LINE-1 and SINE B1. The increased expression of marker genes in dioxin-exposed voles was quite well associated with toxic process’ mechanisms - their formation and progression under exposure of several generations to low sub-toxic doses. The data obtained will contribute to the development of a biomonitoring method for assessing the initial e ects of dioxin ecotoxicity.","PeriodicalId":334823,"journal":{"name":"Vestnik Moskovskogo universiteta. Seria 16. Biologia","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124578696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Stakhanova, O. Voskresenskaya, V. Golubovich, A. Kamensky
{"title":"THE EFFECT OF THE AC-D-MPRG PEPTIDE BASED ON THE C-TERMINAL FRAGMENT OF ARGININE-VASOPRESSIN(6-9) ON THE CHARACTER OF RATS OF DIFFERENT AGES","authors":"A. Stakhanova, O. Voskresenskaya, V. Golubovich, A. Kamensky","doi":"10.55959/msu0137-0952-16-78-2-1","DOIUrl":"https://doi.org/10.55959/msu0137-0952-16-78-2-1","url":null,"abstract":"RuIn this work, it was found that Ac-D-MPRG, a tetrapeptide synthesized on the basis of the C-terminal fragment of arginine-vasopressin(6-9), upon chronic neonatal administration, a ects anxiety and exploratory behavior in rats of three age groups. The most signi cant effect of the peptide was manifested under more stressful conditions. The rats of the experimental group showed less anxiety. They did less grooming in the Elevated Plus Maze test and increased time in light in the Light-Dark Chamber test. Motor activity changed from passive holding in the prepubertal period to active avoidance in puberty and adulthood. Based on the data obtained, we conclude that the effects of the peptide are manifested only when exposed to a biologically signi cant stimulus.","PeriodicalId":334823,"journal":{"name":"Vestnik Moskovskogo universiteta. Seria 16. Biologia","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116364010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ADAPTATION OF GREEN PHOTOSYNTHETIC BACTERIA TO DIFFERENT ILLUMINATION INTENSITY ACCORDING TO SPECTROSCOPY DATA ON CHLOROSOMES","authors":"А.G. Yakovlev, А.S. Таisova, Z. Fetisova","doi":"10.55959/msu0137-0952-16-78-2-2","DOIUrl":"https://doi.org/10.55959/msu0137-0952-16-78-2-2","url":null,"abstract":"The absorption and circular dichroism spectra of chlorosomes isolated from green photosynthetic bacteria Chloroflexus aurantiacus grown under di erent illumination were studied. It was found that as the intensity of the growth light decreases, the spectra shift to the red side and become narrower and larger in amplitude. Theoretical modeling of the data obtained was performed using the theory of excitons. The conclusion was formulated that the number of bacteriochlorophyll c molecules in linear chains, which form the basis of the elementary blocks of chlorosomes, become greater as the intensity of light at which bacteria are grown decreases. It was suggested that this phenomenon increases the e ciency of capturing weak light uxes and thereby increases the chances of bacterial survival in conditions of sunlight de ciency.","PeriodicalId":334823,"journal":{"name":"Vestnik Moskovskogo universiteta. Seria 16. Biologia","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121328820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Osmolovsky, A. Shestakova, D.Ye. Surkova, R. Lehotská, E. Piecková
{"title":"INVESTIGATION OF NEW ASPERGILLUS STRAINS AS PRODUCERS OF HEMOSTATICALLY ACTIVE PROTEASES","authors":"A. Osmolovsky, A. Shestakova, D.Ye. Surkova, R. Lehotská, E. Piecková","doi":"10.55959/msu0137-0952-16-78-2-7","DOIUrl":"https://doi.org/10.55959/msu0137-0952-16-78-2-7","url":null,"abstract":"For ve species of Aspergillus micromycetes, the diversity of proteolytic activities against human hemostasis proteins was assessed. For every strain, absence of activities similar to hemostatic proteases - thrombin, plasmin, factor Xa, urokinase, and protein C was shown. In addition, no activating activities towards precursors of the named proteases were demonstrated. For A. glaucus, proteases of high activity (58,52 Utyr) and speci city against brinogen were found. A. athecius, A. caespitosus и A. wentii, were capable of proteolysis at acidic pH.","PeriodicalId":334823,"journal":{"name":"Vestnik Moskovskogo universiteta. Seria 16. Biologia","volume":"110 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124726989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}