Folia Medica Indonesiana最新文献

{"title":"Antifungal Activity of Endophytic Bacteria Isolated from Miana Plants (Coleus scutellarioides (L.) Benth.) against Candida albicans","authors":"Melda Yunita, Ruth Magdalena Lumbantobing, Ritha Tahitu","doi":"10.20473/fmi.v59i3.43989","DOIUrl":"https://doi.org/10.20473/fmi.v59i3.43989","url":null,"abstract":"Highlights: 1.Due to the adverse effects associated with current antifungal drugs, research on traditional medicine is necessary to explore other options for candidiasis treatment.2.Following the findings of this study, it is recommended to conduct further research by incorporating Coleus scuttellarioides filtrate into the growth medium, since this may improve bacterial growth by producing optimal secondary metabolites.   Abstract Candida albicans is the most common organism responsible for both mucosal and systemic infections, accounting for approximately 70% of fungal infections worldwide. Miana, scientifically known as Coleus scuttellarioides (L.) Benth., is recognized for its use in traditional medicinal practices. Miana plants contain endophytic bacteria that possess the ability to produce secondary metabolites with potential antifungal agents. The objective of this study was to assess the antifungal activity of nine endophytic bacteria isolates derived from Coleus scuttellarioides against Candida albicans. This study was laboratory-based qualitative experimental research that applied the Kirby-Bauer diffusion method and several modifications. The Candida albicans specimens were spread throughout the entire potato dextrose agar medium. Afterwards, paper discs that had been soaked in a liquid culture of endophytic bacterial isolates were carefully placed on the surface of the medium. The complete setup was then incubated for 1–2 days. The potential antifungal activity of endophytic bacteria was assessed by observing the emergence of a clear zone surrounding their growth, which would indicate inhibition. An additional observation was performed in the follow-up test, involving the use of Sabouraud dextrose agar medium to confirm the initial test result. The results from the inhibitory test revealed that none of the bacterial isolates exhibited any inhibition zone. Conversely, ketoconazole as the positive control showed an inhibition zone with an average diameter of 28.5 mm. In conclusion, endophytic bacterial isolates obtained from Coleus scuttellarioides have no discernible antifungal properties against Candida albicans. This study implies that ketoconazole remains effective in treating infections caused by Candida albicans.","PeriodicalId":32666,"journal":{"name":"Folia Medica Indonesiana","volume":"148 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139341273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofilm Formation in Staphylococcus aureus and Coagulase-Negative Staphylococcus","authors":"Marta Setiabudy, Dewa Ayu Putri Sri Masyeni, Anak Agung Gede Indraningrat, Kadek Suryawan, I Ketut Agus Indra Adhiputra, Muhammad Amirul bin Abdul Rahman","doi":"10.20473/fmi.v59i3.44598","DOIUrl":"https://doi.org/10.20473/fmi.v59i3.44598","url":null,"abstract":"Highlights: 1. The significance of Staphylococcus aureus and coagulase-negative Staphylococcus, which are more likely to infectimmunocompromised patients, needed to be researched in greater depth.2. Coagulase-negative Staphylococcus was found to form significantly more biofilm than Staphylococcus aureus.3. Wound care and changing medical devices in immunocompromised patients on a regular basis may provide benefitsto prevent biofilm formation by Staphylococcus spp.   Abstract Staphylococcus spp. are typically commensal microorganisms that can exist in the human body without causing illness. However, these bacteria have virulence factors, e.g., biofilm formation, that are important to note. Because biofilms shield bacteria from opsonophagocytosis and antimicrobial agents, they can cause persistent or chronic infections. Once they form biofilms, both Staphylococcus aureus and coagulase-negative Staphylococcus (CoNS) can potentially cause incurable infections. This study aimed to compare biofilm formation in Staphylococcus aureus and coagulase-negative Staphylococcus as a guide for the prevention and management of infection, which will maintain and improve the good health of the general population. This was an analytic research with a cross-sectional design. The study began by collecting the samples, identifying the species, and testing the biofilm production with a microtiter plate, which was then analyzed with an enzyme-linked immunosorbent assay (ELISA). Data analysis was conducted using IBM SPSS Statistics for Windows, version 25.0 (IBM Corp., Armonk, N.Y., USA). Comparison tests were conducted using an independent t-test. A value of p<0.05 was used as the cut-off that indicated significance. The total samples were 36 clinical isolates, consisting of 18 Staphylococcus aureus and 18 coagulase-negative Staphylococcus. The specimens consisted of 20 blood samples (55.6%) and 7 wound swabs (19.4%). The biofilm test on the samples showed that 83.3% of the samples produced biofilms. The data revealed that the isolates formed biofilms, with 14 isolates (38.9%) in the strong category, 10 isolates (27.8%) in the moderate category, and each of 6 isolates (16.7%) in the weak and non-existent categories. Both Staphylococcus spp. appeared to have biofilm-forming activity, but coagulase-negative Staphylococcus appeared to be significantly more dominant (p=0.008). Strong biofilm was produced by 61.1% of coagulase-negative Staphylococcus isolates. In conclusion, coagulase-negative Staphylococcus formed a stronger biofilm than Staphylococcus aureus. Its presence as an infection-causing bacteria, particularly in immunocompromised patients, should not be underestimated.","PeriodicalId":32666,"journal":{"name":"Folia Medica Indonesiana","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139341309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metformin Effectiveness in Reducing Mortality among Covid-19 Patients with Type 2 Diabetes Mellitus at a Tertiary Hospital in Indonesia","authors":"Yudivaniel Zihono, Hany Yusmaini, Uswatun Hasanah, Erna Harfiani, Md Ikhsan Mokoagow, Dicky Budiman","doi":"10.20473/fmi.v59i3.46944","DOIUrl":"https://doi.org/10.20473/fmi.v59i3.46944","url":null,"abstract":"Highlights: 1. As there is a scarcity of publications on the use of metformin for COVID-19 in Indonesia, the findings of this present study may contribute more insight to the existing body of research and provide data specific to the Southeast Asian population.2. This study revealed a decreased mortality rate associated with metformin use in diabetic patients with mild to moderate COVID-19 infection.3. This study suggests that diabetic patients may continue metformin treatment during a COVID-19 infection as the medication has sustained therapeutic effects.   Abstract COVID-19 patients with comorbidities, such as type 2 diabetes (T2DM), have a higher mortality rate compared to those without any comorbidities. T2DM patients usually receive metformin as their first-line treatment. However, the effectiveness of metformin in reducing mortality rates still requires further analysis. The objective of this study was to analyze the effectiveness of metformin in reducing mortality rates among COVID-19 patients with T2DM. An analytic observational design with a retrospective cohort approach was used in this study. Samples were acquired from hospitalized COVID-19 patients with T2DM medical records at Fatmawati Central General Hospital, Jakarta, Indonesia, throughout 2020–2021. The samples were collected using a purposive sampling technique and analyzed using Chi-square test (p<0.05; RR<1). This study comprised 137 samples, with 56 samples receiving metformin and 81 not receiving metformin. The mortality rate in the sample group that received metformin was lower (19.6%) compared to the group that was not given the medication (38.3%). The Chi-square test results indicated a statistically significant relationship between metformin treatment and a lower mortality rate among COVID-19-contracted individuals with T2DM (p=0.020; RR=0.513). Therefore, this study concludes that the administration of metformin treatment reduces mortality among COVID-19 patients with T2DM.","PeriodicalId":32666,"journal":{"name":"Folia Medica Indonesiana","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139341375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity Test on the Combination of Caesalpinia sappan and Zingiber officinale in Rattus norvegicus Induced by Complete Freund's Adjuvant","authors":"Nadiah Armadanti Salma, Tukiran, Suyatno Sutoyo, Fauzia Indah Sabila","doi":"10.20473/fmi.v59i3.44455","DOIUrl":"https://doi.org/10.20473/fmi.v59i3.44455","url":null,"abstract":"Highlights:1. This study determined the toxic effects of combining Caesalpinia sappan and Zingiber officinale extracts, aspeople need to be aware of the potential side effects of these common herbal remedies for the treatment ofrheumatoid arthritis.2. The combination of Caesalpinia sappan and Zingiber officinale extracts is efficacious and non-toxic as an antiarthritis treatment, hence, the ingredients can be upgraded to standardized herbal medicines andphytopharmaceuticals.   Abstract Sappanwood (Caesalpinia sappan) and red ginger (Zingiber officinale) are plant species that have been studied for theirefficacy in treating inflammation related to rheumatoid arthritis. This study aimed to examine the effects of combiningsappanwood and red ginger in order to determine the potential toxicity of the herbal extracts in medicine. The toxicitytesting was carried out in vivo using 32 Wistar strain male white rats (Rattus norvegicus) grouped into eight groups of four.The rats were injected with complete Freund's adjuvant to induce a chronic inflammatory effect. The eight groups consistedof the negative control group, the positive control group, the normal group, and five treatment groups. This study wasconducted by observing the animals for toxic symptoms and death to determine the safety of the extracts and drugs. Theobservation results were analyzed using a one-way analysis of variance (p<0.05). The analysis results showed that weightgain and relative organ weight among the groups had no significant differences (p>0.05). Microscopic examination of theorgan preparations observed under a light microscope revealed no significant changes or adverse effects in rats treated withthe extracts or drugs. In conclusion, a combination of sappanwood and red ginger ethanol extracts administered orally hasno toxic effect in rats injected with complete Freund's adjuvant.","PeriodicalId":32666,"journal":{"name":"Folia Medica Indonesiana","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139341380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Moringa oleifera Extract as an Immunomodulator of Lymphocyte Cells and Macrophages in BALB/c Mice Infected with Plasmodium berghei","authors":"Putu Khrisna, Dharma Jaya, Budi Apsari, Pande Made, Alitta Cantika Putri, Nadya Dewi, Dewa Ayu, Agus Sri Laksemi, Ketut Cahyadi, Adi Winata Sutarta, Putu Indah, Budiapsari","doi":"10.20473/fmi.v59i3.45237","DOIUrl":"https://doi.org/10.20473/fmi.v59i3.45237","url":null,"abstract":"Highlights: 1. This study assessed the immunomodulatory potential of Moringa oleifera, which may serve as a natural source forantimalarial treatment.2. Moringa oleifera extract can act as an immunomodulator due to its suppressive effect on Plasmodium berghei infection.   Abstract Malaria is an infectious disease caused by protozoa of the genus Plasmodium. In Indonesia, this disease remains a health concern that must be resolved. Due to its high prevalence in eastern Indonesia, it is a challenge to eradicate this disease. Moringa oleifera contains various substances that are believed to have antimalarial activity. This study aimed to determine the effectiveness of Moringa oleifera leaf extract in increasing immune cells and eradicating parasites by using mice infected with Plasmodium berghei. The research was conducted in vivo on BALB/c strain mice (n=40) that were already infected with Plasmodium berghei. Moringa oleifera leaf extract at 25%, 50%, and 75% concentrations was administered orally every day to the mice, while a peripheral blood smear was performed to evaluate parasitemia levels and macrophage activation. A complete blood count was also performed after all tests on the mice were completed. Statistical analysis was performed using the one-way analysis of variance (ANOVA) test with a=0.05 and 95% confidence interval (CI). The results showed that the administration of Moringa oleifera leaf extract at 25%, 50%, and 75% concentrations caused varying degrees of parasitemia compared to the negative group (p<0.05). The group that received the extract at 50% concentration differed significantly from the control groups in the number of activated macrophages. The results of the complete blood count indicated immunomodulatory effects through the presence of diverse immune cell types. In conclusion, Moringa oleifera leaf extract suppresses Plasmodium berghei infection and enhances immune cell stimulation.","PeriodicalId":32666,"journal":{"name":"Folia Medica Indonesiana","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139341295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemoglobin A1c (HbA1c) Levels from the Examination of Different Blood Proportions in K2EDTA Tubes using an Enzymatic Method","authors":"Museyaroh, Musholli Himmatun, Nabilah Retno, Werdiningsih, Nabilah","doi":"10.20473/fmi.v59i3.40652","DOIUrl":"https://doi.org/10.20473/fmi.v59i3.40652","url":null,"abstract":"Highlights: 1. Research on the effect of blood volume proportion in the examination of HbA1c levels using K2EDTA anticoagulantvhas yet to be widely carried out in Indonesia.2. The analysis conducted utilizing K2EDTA tubes revealed that the varying proportions of blood samples had no effectvon HbA1c levels.3. This article highlights the necessity of evaluating the pre-analytical phase (sample preparation) of a laboratory process to improve the accuracy of the results and minimize false high or low results in the HbA1c test.   Abstract Hemoglobin A1c (HbA1c) examination is the gold standard for diagnosing and monitoring diabetes mellitus patients. In the examination, the pre-analytical phase has the most considerable error rate at 61%. One of the contributing factors is the use of anticoagulants that do not adhere to established guidelines. Additionally, the incompatibility of the sample volume proportions and the anticoagulants in K2EDTA tubes has been observed in numerous cases. The significance of HbA1c testing, particularly in the prevention of diabetes complications, underscores the need for appropriate procedures to be followed throughout the sampling and pre-analytical phases. This study aimed to determine the effect of differences in the proportion of blood sample volume and anticoagulants in K2EDTA tubes on HbA1c levels. This research was conducted in August–September 2022. The research samples were collected from the staff and students at the Department of Medical Laboratory Technology, Politeknik Kesehatan Kemenkes Surabaya, Surabaya, Indonesia. The blood samples were divided into K2EDTA tubes with varying volumes of 1 mL, 2 mL, 3 mL, and 4 mL. The HbA1c levels were then examined at the Clinical Chemistry Laboratory of Politeknik Kesehatan Kemenkes Surabaya. The data were analyzed using a one-way ANOVA test. The statistical test results (p>0.05) indicated that the proportion of samples containing anticoagulants in the K2EDTA tubes did not have any significant effect on HbA1c levels. In conclusion, it is acceptable to utilize K2EDTA tubes with varying blood sample volumes for the measurement of HbA1c levels.","PeriodicalId":32666,"journal":{"name":"Folia Medica Indonesiana","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139341305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Childhood Obesity on Psychomotor Behavior","authors":"Muhammad Adrian Khalfani, Purwo Sri Rejeki, Sakina, Nur Ezza Fazleen Mohd Fathil","doi":"10.20473/fmi.v59i3.45280","DOIUrl":"https://doi.org/10.20473/fmi.v59i3.45280","url":null,"abstract":"Childhood obesity is a complex problem, and its prevalence among school-age children has been found to have a greater impact on their physical well-being compared to their learning difficulties. In some cases, teachers or schools may not pay enough attention to issues that have the potential to affect or delay the psychomotor development of children. Therefore, this study aimed to provide enhanced comprehension regarding the effect of childhood obesity on psychomotor behavior. This study used a systematic review methodology to synthesize information regarding the effect of obesity on children's health and development. The information and data presented in this study were obtained from several scientific sources accessed through online libraries such as PubMed, ScienceDirect, and the Cochrane Library. A comprehensive search was conducted to identify scholarly publications, which resulted in a total of 815 papers published between 2013 and 2022. The selected papers exclusively consisted of original research articles that primarily focused on investigating the relationship between obesity in children aged 5–14 years and their psychomotor abilities and development outcomes. Following the screening process, five studies were found to meet the specified criteria. The findings of the selected studies revealed a substantial resemblance, specifically the correlation between childhood obesity and poor motor skills. Furthermore, the selected studies discovered that an increase in body fat is commonly associated with a rise in total body weight, or the relative body mass index (BMI), in both adults and children. Some studies demonstrated significant differences in the performance of diverse psychomotor variables according to children's BMI. According to the results of the studies, children with a higher BMI showed inferior performance in motor activities as a result of compromised body control and balance that inhibited the children's movement. This systematic review concludes that obesity has an effect on the psychomotor behavior of children.","PeriodicalId":32666,"journal":{"name":"Folia Medica Indonesiana","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139341325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Formulae to Determine Living Stature using Handprint Anthropometry of Tagalog People in the Philippines","authors":"Tharmar Nataraja Moorthy, Ivan Nikkimor, Lao Dinglasa, Myrtati Dyah Artaria, Tharmar Nataraja, Moorthy","doi":"10.20473/fmi.v59i3.47573","DOIUrl":"https://doi.org/10.20473/fmi.v59i3.47573","url":null,"abstract":"Highlights: 1. This is the first-ever anthropological study on Tagalog people in the Philippines that has established formulae for determining stature using handprint length measurements.2. This study has generated formulae that are applicable for personal identification purposes within real crime scenes.   Abstract Forensic science plays a crucial role in the pursuit of justice, particularly through the identification of physical evidence found at crime scenes, such as human fingerprints and handprints. This study aimed to develop formulae for determining living stature using the handprint anthropometry of Tagalog people, an indigenous ethnic group in the Philippines. A total of 360 Tagalog volunteers, comprising 180 men and 180 women, were recruited. This study excluded subjects who had finger and hand-related diseases, injuries, or were under the age of 18. The materials used were a stadiometer for height measurement, a digital vernier caliper for handprint measurements, and a handprint kit to collect handprints. Five length measurements were collected for each handprint. The length measurement spanned the distance from the middle wrist crease to the tips of each of the five fingers. The data were analyzed statistically using regression analysis (p<0.05) in IBM SPSS Statistics for Windows, version 26.0 (IBM Corp., Armonk, N.Y., USA). The analysis results produced equations for determining stature using all the length measurements of the handprints. The study involved the calculation of correlation coefficients (r values) and standard deviations using the stature and handprint lengths of individuals of both genders. The results are presented in the form of tables and figures. The study concluded with the development of regression equations that may be utilized for determining stature based on various handprint length measurements of the Tagalog people. This study represents the first-ever anthropological study conducted on the Philippine Tagalog population within the scope of this research subject matter. The formulae can be applied to actual crime scenes for the purpose of personal identification.","PeriodicalId":32666,"journal":{"name":"Folia Medica Indonesiana","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139341349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Auricular Acupressure Effectively Reduces Labor Anxiety in Primigravidae by Increasing β-Endorphin Levels","authors":"Setiawandari, Koosnadi Saputra, Yuni Khoirul Waroh, Setiana Andarwulan","doi":"10.20473/fmi.v59i3.46738","DOIUrl":"https://doi.org/10.20473/fmi.v59i3.46738","url":null,"abstract":"Highlights: 1. Auricular acupressure at the Shenmen point is effective in reducing anxiety.2. Auricular acupressure is more practical than body acupressure since it does not interfere with expectant mothers' mobilization.3. As a non-pharmacological method, auricular acupressure is cost-effective, efficient, and effective in overcoming maternal anxiety during labor   Abstract Anxiety is a common psychological symptom in expectant mothers, particularly among primigravidae during labor. There is a link between excessive anxiety and prolonged labor. One of the options to manage anxiety is the use of non-pharmacological methods. Numerous studies have investigated the effectiveness of auricular acupressure to treat anxiety, but few have examined its effect on anxiety biomarkers. Thus, the objective of this study was to analyze the effect of auricular acupressure targeting the Shenmen point on the levels of β-endorphins and anxiety among primigravidae during the first stage of labor. This experimental study used a pretest-posttest control group design. The research subjects were primigravidae in the first stage of labor at the independent midwife practice of Yefi Marliandiani in Surabaya, Indonesia. Among 40 primigravidae, 34 individuals met the inclusion criteria and were then equally divided into treatment and control groups using a simple randomization. The treatment group received auricular acupressure at the Shenmen point every 30 minutes for a duration of 5 minutes, while the control group practiced deep breathing relaxation. The anxiety levels were assessed using the Hamilton Anxiety Rating Scale. The β-endorphin levels were measured by drawing 3 cc of venous blood before (4 cm cervix dilation) and after (9–10 cm cervix dilation) treatment. The obtained data were analyzed using paired- and independent-samples t-tests. A decrease in the mean anxiety level was observed in the treatment group (18.59±7.52) relative to the control group (19.76±7.11), albeit not significant (p=0.64). Simultaneously, the average β-endorphin levels of the treatment group (355.4±224.7) increased compared to the control group (225.1±127.5), with a significant difference observed between the two groups (p=0.04). In conclusion, while both auricular acupressure at the Shenmen point and deep breathing relaxation are effective in reducing anxiety, the former is more prominent in its ability to increase β-endorphin levels.","PeriodicalId":32666,"journal":{"name":"Folia Medica Indonesiana","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139341363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact on the Kidney of Pancreas Damage due to Streptozotocin-Induced Hyperglycemia","authors":"Yana Aurora Prathita, A. A. Jusuf, Christina Simadibrata, Wahyuningsih Djaali, Yoshua Viventius","doi":"10.20473/fmi.v59i2.33584","DOIUrl":"https://doi.org/10.20473/fmi.v59i2.33584","url":null,"abstract":"Highlights:\u0000\u0000 This study observed the histology of pancreatic β-cell damage without any intervention to the kidneys of the animal models.\u0000 The histological analysis of the kidneys shows that STZ-induced animal models can be used for assessing kidney abnormalities due to hyperglycemia.\u0000A scoring system for the histological analysis was developed to evaluate the changes in the kidney cells.\u0000\u0000Abstract\u0000The kidneys are one of the organs affected by microvascular complications due to diabetes mellitus. Hyperglycemia plays an important role in glomerular, mesangial cell, and tubular damage in the kidneys. Metabolic dysregulation, including hyperglycemia, initiates cellular damage in the kidneys. Streptozotocin (STZ) is a chemical compound that is known to damage pancreatic cells and cause hyperglycemia. This study aimed to examine the effects of hyperglycemia on the morphology of the kidneys. Kidney tissues were observed histologically using a light microscope. Samples were taken from the kidneys of experimental animals administered with STZ to induce hyperglycemia. Observation was performed afterwards to investigate any damage to pancreatic cells. A total of 12 kidney samples were divided into two groups: the control group and the STZ-induced group. The samples were prepared before staining with hematoxylin-eosin and Masson's trichrome. The endothelium, podocytes, mesangial cells, and basement membrane of the glomerulus were examined. The tubules of the kidneys were also examined, and the presence or absence of connective tissue formation in both groups was statistically tested. The results suggested a significant difference in tubular damage (p<0.05) and an insignificant difference in an increase in the damage of other components of the kidneys (p>0.05) in the STZ-induced group. Significant morphological changes were observed in the hyperglycemic renal tubules due to the administration of STZ. In conclusion, STZ-induced hyperglycemia caused damage to the kidney components but overall had no significant impact on the kidney. ","PeriodicalId":32666,"journal":{"name":"Folia Medica Indonesiana","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41623313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}