Jurnal Fitopatologi Indonesia最新文献_第2页

Antifungal Activity of Leaf Extract of Alchornea cordifolia Against Aspergillus flavus the causal Agent of Yam Tuber Rot 胡枝子叶提取物对山药块茎腐烂病病原菌黄曲霉的抗真菌活性

Jurnal Fitopatologi Indonesia Pub Date : 2023-12-04 DOI: 10.14692/jfi.19.6.253-264

D. Enyiukwu, A. C. Amadioha, I. Bassey

{"title":"Antifungal Activity of Leaf Extract of Alchornea cordifolia Against Aspergillus flavus the causal Agent of Yam Tuber Rot","authors":"D. Enyiukwu, A. C. Amadioha, I. Bassey","doi":"10.14692/jfi.19.6.253-264","DOIUrl":"https://doi.org/10.14692/jfi.19.6.253-264","url":null,"abstract":"Rots and mycotoxin contamination of agro-produce are prevalent in tropical world. Aspergillus flavus is a major mycotoxigenic rot contaminant of tubers in Abia state, Nigeria. Consumption of mycotoxin contaminated foodstuffs accounts for 25% of medically important human diseases in Nigeria. The objective of this study was to assess antifungal activity of Alchornea cordifolia against A. flavus a mycotoxigenic rot pathogen of stored tuber produce both in vitro and in vivo. Isolates were made from rotted yam tubers and pathogenicity test carried out to confirm A. flavus as pathogen. Five concentrations (100-500 mg mL-1) of methanol leaf extracts of the plant and thiophanate-methyl–a standard fungicide–were evaluated against the growth of the fungus in culture and pathogen-induced rot development and spread in vivo. The experiment was made up of 7 treatments and 3 replicates laid out in CRD. The plant leaf extract demonstrated varying levels of inhibition of A. flavus in vitro and rot development and spread in living tissues of yam. About 56.38%–68.22% and 67.245–80.01% inhibition was recorded by 100 mg mL-1 and 500 mg mL-1 of A. cordifolia extract for spore germination respectively whereas the same concentrations reduced rot development from 60% in the control experiment to 21.80% and 12.10% respectively which compared favorably (P<0.05) with standard fungicide, thiophanate-methyl. The plant demonstrated strong antifungal activity in vitro and minimized A. flavus induced rot advancement in living yam tissues.","PeriodicalId":31619,"journal":{"name":"Jurnal Fitopatologi Indonesia","volume":"51 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138602133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathogenicity of Botryodiplodia theobromae on Sandalwood Stems and Its in Vitro Inhibition by Trichoderma spp. Botryodiplodia theobromae 在檀香树茎上的致病性及其在体外对毛霉菌属的抑制作用

Jurnal Fitopatologi Indonesia Pub Date : 2023-11-29 DOI: 10.14692/jfi.19.6.238-245

Sane Wolagole, Agnes Virginia Simamora, M. V. Hahuly

引用次数: 0

Morphometric Analysis of Chili Leaves with Yellow Curly Symptom Using Digital Image Processing Approach and Data Mining Algorithm 利用数字图像处理方法和数据挖掘算法对有黄色卷曲症状的辣椒叶进行形态计量分析

Jurnal Fitopatologi Indonesia Pub Date : 2023-11-28 DOI: 10.14692/jfi.19.6.231-237

Asmar Hasan, Muhammad Taufik, L. O. S. Bande, Andi Khaeruni, Rahayu Mallarangeng, Gusnawaty HS, Asniah, Syair, Abdul Rahman

{"title":"Morphometric Analysis of Chili Leaves with Yellow Curly Symptom Using Digital Image Processing Approach and Data Mining Algorithm","authors":"Asmar Hasan, Muhammad Taufik, L. O. S. Bande, Andi Khaeruni, Rahayu Mallarangeng, Gusnawaty HS, Asniah, Syair, Abdul Rahman","doi":"10.14692/jfi.19.6.231-237","DOIUrl":"https://doi.org/10.14692/jfi.19.6.231-237","url":null,"abstract":"Gejala kuning keriting pada daun cabai umumnya disebabkan oleh infeksi Begomovirus. Daun tanaman terinfeksi tidak hanya mengalami perubahan warna sebagai indikator rusaknya klorofil tetapi juga mengalami perubahan morfologi bentuk. Penelitian ini bertujuan menguantifikasi gejala infeksi Begomovirus berdasarkan perubahan morfologi bentuk daun menggunakan pengolahan citra digital dan algoritma data mining yang akan memudahkan dalam pemantauan dan analisis perkembangan penyakit tanaman. Total 33 citra daun cabai rawit bergejala kuning keriting maupun tidak bergejala menjadi dataset penelitian ini. Citra daun cabai tersebut diolah dan diekstrak karakteristik bentuknya berupa circularity, aspect ratio, roundness, dan solidity menggunakan aplikasi Fiji-ImageJ. Selanjutnya dilakukan uji beda (uji-t), pengelompokan citra menggunakan algoritma Simple K-Means, dan evaluasi ketepatan hasil pengelompokan berdasarkan indeks ARI dan NMI. Hasil penelitian menunjukkan bahwa secara umum ada perbedaan bentuk yang nyata antara daun bergejala dengan daun tidak bergejala. Daun cabai rawit bergejala kuning keriting memiliki rata-rata nilai aspect ratio dan solidity yang lebih kecil dibandingkan daun cabai tidak bergejala, sebaliknya memiliki rata-rata nilai circularity dan roundness yang lebih besar dibandingkan daun cabai tidak bergejala. Evaluasi ketepatan pengelompokan sampel daun cabai rawit bergejala maupun tidak bergejala berdasarkan indeks ARI dan NMI menghasilkan nilai terbaik untuk pengelompokkan ke dalam dua kelompok.","PeriodicalId":31619,"journal":{"name":"Jurnal Fitopatologi Indonesia","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139226665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Etiology of Anthracnose Disease on Shallots Caused by Colletotrichum gloeosporioides 青葱炭疽病菌的病原学研究

Jurnal Fitopatologi Indonesia Pub Date : 2023-11-03 DOI: 10.14692/jfi.19.5.206-214

Rizki Abi Amrullah, Suryo Wiyono, Awang Maharijaya, Agus Purwito

引用次数: 0

Detection and Practical Differentiation of Phytoplasmas from Several Host Plants Using PCR-RFLP 利用PCR-RFLP技术检测几种寄主植物的植物原体并进行实用分化

Jurnal Fitopatologi Indonesia Pub Date : 2023-10-31 DOI: 10.14692/jfi.19.5.188-195

Kikin Mutaqin, Purnama Hidayat, Budi Tjahjono, Yayi Munara Kusumah, Rusmilah Suseno

{"title":"Detection and Practical Differentiation of Phytoplasmas from Several Host Plants Using PCR-RFLP","authors":"Kikin Mutaqin, Purnama Hidayat, Budi Tjahjono, Yayi Munara Kusumah, Rusmilah Suseno","doi":"10.14692/jfi.19.5.188-195","DOIUrl":"https://doi.org/10.14692/jfi.19.5.188-195","url":null,"abstract":"Phytoplasma as a phytopathogenic prokaryote with a wide host range is a pathogen that needs more attention in Indonesia. This pathogen is relatively difficult to detect and identify due to its complicated biological properties. This study involved detection of phytoplasmas by polymerase chain reaction (PCR) technique with P1/P7 primers from seven symptomatic plants, i.e. Bermuda grass white leaf, bamboo yellows, witches’ broom of peanut, soybean, yard long bean, and cactus, and sweet potato little leaf. The phytoplasma DNA of the 16S rRNA gene resulting from PCR amplification was examined by digestion reaction using three endonuclease enzymes AluI, RSaI, and MSeI to generate restriction fragment length polymorphism (RFLP) profile. The seven diseased plants were confirmed positive to be associated with phytoplasma as indicated by the PCR product of 1800 bp. Based on the RFLP profiles of the three enzymes, the phytoplasmas were divided into two groups, namely group I (Bermuda grass and bamboo) and group II (peanuts, soybeans, yard long beans, cactus, and sweet potatoes). Cactus phytoplasma is a sub-group (strain) because it has a slightly different fragment of MSeI RFLP profile.","PeriodicalId":31619,"journal":{"name":"Jurnal Fitopatologi Indonesia","volume":"67 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135814865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Low Temperature and Antiviral for Elimination of Chrysanthemum stunt viroid and Its Effect on the Growth of Shoot Tip Culture of Chrysanthemum 低温和抗病毒技术对菊苗类病毒的去除及其对菊茎尖培养苗生长的影响

Jurnal Fitopatologi Indonesia Pub Date : 2023-10-31 DOI: 10.14692/jfi.19.5.196-205

Erniawati Diningsih, Safani Aryantika, Indijarto Budi Rahardjo, Wakiah Nuryani, None Hanudin, Ifa Manzila

{"title":"Application of Low Temperature and Antiviral for Elimination of Chrysanthemum stunt viroid and Its Effect on the Growth of Shoot Tip Culture of Chrysanthemum","authors":"Erniawati Diningsih, Safani Aryantika, Indijarto Budi Rahardjo, Wakiah Nuryani, None Hanudin, Ifa Manzila","doi":"10.14692/jfi.19.5.196-205","DOIUrl":"https://doi.org/10.14692/jfi.19.5.196-205","url":null,"abstract":"Chrysanthemum stunt viroid (CSVd) telah banyak dilaporkan menjadi salah satu faktor pembatas dalam usaha produksi bunga potong krisan. Penelitian bertujuan untuk mengetahui pengaruh perlakuan suhu rendah (5 ℃) dan antiviral ribavirin terhadap pertumbuhan dan pembebasan CSVd pada planlet krisan yang dikultur dari ujung tunas. Tanaman terinfeksi CSVd diperoleh dari kebun percobaan milik pemerintah di Cianjur, Jawa Barat. Penelitian dilakukan dengan beberapa tahap, yaitu 1) pengambilan sampel tanaman, 2) deteksi viroid dengan metode RT-PCR, 3) inisiasi eksplan dan perbanyakkan bahan tanaman secara in vitro, 4) perlakuan suhu rendah 5 ℃ dengan tiga taraf waktu inkubasi (1, 3, dan 5 bulan), 5) perlakuan antiviral ribavirin dengan tiga taraf konsentrasi (25, 100, dan 125 ppm), dan 6) konfirmasi bahan tanaman bebas viroid dengan RT-PCR. Hasil penelitian menunjukkan bahwa penyimpanan suhu rendah dan perlakuan ribavirin menurunkan laju pertambahan tinggi tunas dan jumlah daun. Sampai pada konsentrasi 125 ppm, walaupun menurunkan laju pertumbuhan tunas dan jumlah daun, ribavirin tidak menimbulkan toksisitas terhadap tanaman. Akan tetapi, kombinasi perlakuan suhu rendah (1, 3, dan 5 bulan) dengan ribavirin (25, 100, dan 125 ppm) belum mampu mengeliminasi CSVd dari jaringan pada semua taraf perlakuan.","PeriodicalId":31619,"journal":{"name":"Jurnal Fitopatologi Indonesia","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135869452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The First Occurrence of Zucchini yellow mosaic virus Infecting Cucumber in Padang, West Sumatra 西苏门答腊岛巴东地区首次发生黄瓜黄花叶病毒感染

Jurnal Fitopatologi Indonesia Pub Date : 2023-10-30 DOI: 10.14692/jfi.19.5.183-187

Muhammad Arif Ridho, None Martinius, None Fadli, Yenny Liswarni, Lailatul Najmi, Jumsu Trisno

引用次数: 0

Cover Jurnal Fitopatologi Vol. 19 No. 4, Juli 2023 封面 Jurnal Phytopathologi 第 19 卷第 4 期，2023 年 7 月

Jurnal Fitopatologi Indonesia Pub Date : 2023-10-24 DOI: 10.14692/jfi.19.4.i

None Editors Jurnal Fitopatologi Indonesia

引用次数: 0

Combination of Barrier Corp, Chitosan, Bougaivillea Leaf Extract to Manage Virus on Soybean 屏障剂、壳聚糖、三角梅叶提取物联合防治大豆病毒

Jurnal Fitopatologi Indonesia Pub Date : 2023-09-27 DOI: 10.14692/jfi.19.4.166-175

Iwe Cahyati, Tri Asmira Damayanti, Sugeng Santoso

{"title":"Combination of Barrier Corp, Chitosan, Bougaivillea Leaf Extract to Manage Virus on Soybean","authors":"Iwe Cahyati, Tri Asmira Damayanti, Sugeng Santoso","doi":"10.14692/jfi.19.4.166-175","DOIUrl":"https://doi.org/10.14692/jfi.19.4.166-175","url":null,"abstract":"Infeksi virus menjadi salah satu pembatas produksi kedelai di Indonesia. Penelitian bertujuan menguji efektivitas kitosan, ekstrak daun bugenvil, dan kombinasinya, serta insektisida dalam mengendalikan infeksi virus pada kedelai di lapangan. Efektivitas perlakuan diuji dalam petak utama tanpa dan dengan pembatas jagung. Peubah penyakit, populasi serangga, dan peubah agronomi diamati mingguan. Populasi kutudaun pada perlakuan kontrol dengan tanaman pembatas lebih rendah daripada perlakuan kontrol tanpa tanaman pembatas, menunjukkan tanaman pembatas mampu menghalangi kutudaun masuk ke pertanaman kedelai. Inkorporasi perlakuan kitosan, ekstrak daun bugenvil, dan kombinasinya mampu menekan populasi kutudaun sebanding dengan perlakuan insektisida pada kedua petak utama. Area under population progress curve (AUPPC) kutudaun selaras dengan lebih rendahnya area under disease progress curve (AUDPC), insidensi dan keparahan penyakit serta AUDPC secara nyata dibandingkan dengan kontrol tanpa perlakuan. Efektivitas perlakuan pada petak dengan tanaman pembatas mampu menurunkan populasi kutudaun, insidensi dan keparahan penyakit lebih tinggi dibandingkan dengan perlakuan yang sama pada petak tanpa tanaman pembatas. AUDPC antarperlakuan kitosan, ekstrak daun bugenvil, kombinasi, dan insektisida secara umum berbeda nyata lebih rendah pada kedua petak utama dibandingkan dengan kontrol tanpa perlakuan. Hasil panen kedelai menunjukkan lebih tinggi secara signifikan daripada perlakuan kitosan, ekstrak daun bugenvil, dan kombinasinya dibandingkan dengan perlakuan kontrol pada kedua petak utama. Penggunaan tanaman pembatas yang disatukan dengan salah satu perlakuan kitosan dan ekstrak daun bugenvil dapat menjadi paket pengelolaan yang ramah lingkungan untuk mengendalikan infeksi virus pada tanaman kedelai di lapangan.","PeriodicalId":31619,"journal":{"name":"Jurnal Fitopatologi Indonesia","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135538384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perlakuan Air Panas dan Plant Growth Promoting Rhizobacteria untuk Menekan Cendawan Terbawa Benih pada Padi Varietas IPB-3S 热水处理和植物生长促进根瘤菌抑制水稻品种 IPB-3S 的种子真菌

Jurnal Fitopatologi Indonesia Pub Date : 2023-08-30 DOI: 10.14692/jfi.16.6.235-242

Efi Toding Tondok, Desi Andini

引用次数: 0