Journal of Biological Researches最新文献_第8页

Effect of calcusol TM on CuZnSOD expression in mice renal of nephrolothiasis model 钙化醇TM对肾病模型小鼠肾脏CuZnSOD表达的影响

Journal of Biological Researches Pub Date : 2014-10-01 DOI: 10.23869/bphjbr.19.1.20136

Arie Azhari, S. Widyarti

{"title":"Effect of calcusol TM on CuZnSOD expression in mice renal of nephrolothiasis model","authors":"Arie Azhari, S. Widyarti","doi":"10.23869/bphjbr.19.1.20136","DOIUrl":"https://doi.org/10.23869/bphjbr.19.1.20136","url":null,"abstract":"This study aimed to determine the effect of traditional medicine, Calcusol TM , on number of cells expressing CuZnSOD in mice renal of nephrolithiasis model. Eight weeks old Swiss strain male mice ( Mus musculus ) were divided into five groups: (A) control, (B) nephrolithiasis, (C) Calcusol TM , (D) nephrolithiasis & Calcusol TM , and (E) nephrolithiasis & Calcusol TM simultaneously. Nephrolithiasis was induced by applying porang tuber ( Amor-phophallus muelleri ) flour 0.06 mg/g of body weight during 3 months. Calcusol TM is traditional medicine, made of tempuyung leaves extract with S accharum lactis as additional substance. The dosage for Calcusol TM treatment was 3.3 mg/g of body weight. After 3 months treatment, the mice were killed by neck-dislocation, the kidneys were isolated and prepared for paraffin histology. CuZnSOD was analyzed by immunohistochemistry (IHC), using rabbit policlonal antibody anti-SOD1 (Bioss, bs-1079R) as primary antibody. Tissues were observed under Olympus BX51, 400x magnification. Images were documented with Olympus Digital Camera DP20. The histology images were analized in Immunoratio software online (URL: http://153.1.200.58:8080/immunoratio/) to receive the percentage of number of cells expressing CuZnSOD. The result showed that Calcusol TM administration could decrease number of cells expressing CuZnSOD in kidney significantly (P<0.05). It was supposed that antioxidant content in Calcusol TM could scavenge ROS directly with no induction of CuZnSOD production in cell.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"53 1","pages":"26-31"},"PeriodicalIF":0.0,"publicationDate":"2014-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74985602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of genetic variability in soursop Annona muricata L populations from Central Java and East Java based on random amplified polymorphic DNA RAPD marker 基于随机扩增多态性DNA RAPD标记的中爪哇和东爪哇番荔枝居群遗传变异分析

Journal of Biological Researches Pub Date : 2014-08-08 DOI: 10.23869/BPHJBR.19.1.20135

S. Suratman, A. Pitoyo, S. Mulyani

{"title":"Analysis of genetic variability in soursop Annona muricata L populations from Central Java and East Java based on random amplified polymorphic DNA RAPD marker","authors":"S. Suratman, A. Pitoyo, S. Mulyani","doi":"10.23869/BPHJBR.19.1.20135","DOIUrl":"https://doi.org/10.23869/BPHJBR.19.1.20135","url":null,"abstract":"The objective of this research was to determine genetic variability of the soursop (Annona muricata L.) populations from Central Java and East Java based on RAPD markers. Leaves of 40 individuals were collected from 4 soursop populations in Central Java and East Java, include : Sukoharjo, Karanganyar (Central Java), and Ngawi, Pacitan (East Java). Genomic DNA was extracted from the leaves by the CTAB extraction procedure with some modifications. A total of 15 RAPD primers were purchased from commercial source and tested to find specific diagnostic markers for each individuals by RAPD-PCR. The measurement of soursop population genetic distance was based on similarity coefficient using method of Group Average Clustering and Unweight Pair Group Method Arithmetic (UPGMA) of NTSYS program version 2.02i. Results showed that each soursop population collected from different localities seemed have variability in RAPD profiles by using different primers. Four RAPD polymorphic primer was selected from 15 RAPD primers, namely A18, A20, P10 and P11. A total of 58 bands produced, varying from 9 to 20 bands per primer. The selected four RAPD primers produced 57 polymorphic bands, whereas polymorphism for each primer ranged from 95 % to 100 %. Dendrogram indicated that four soursop populations tend to segregate form two separated clade. The sample collected from Sukoharjo formed a separate cluster while the sample collected from Ngawi, Pacitan and Karanganyar grouped together in other cluster and diverged from population Sukoharjo.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"18 1","pages":"15-19"},"PeriodicalIF":0.0,"publicationDate":"2014-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75713605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The effect of bivalve Semele sp meat consumption on estrogen level among the people of Muna Regency Southeast Sulawesi 食用双壳贝对苏拉威西岛东南部穆纳县居民雌激素水平的影响

Journal of Biological Researches Pub Date : 2014-03-15 DOI: 10.23869/bphjbr.18.2.20139

Sjafaraenan Sjafaraenan

引用次数: 4

The effect of biofertilizer fungi on Ciherang rice growth at some level of soil salinity 不同土壤盐分条件下生物肥料真菌对慈和让水稻生长的影响

Journal of Biological Researches Pub Date : 2014-03-15 DOI: 10.23869/BPHJBR.18.2.20136

Y. Subowo

引用次数: 0

CONTAMINATION OF Pb, Cd, Cu, Zn IN BLACK POMFRET (Formio niger) AND TONGUE SAND (Cynoglossus lingua) FISH CAUGHT FROM THE WESTERN PART OF MADURA STRAIT AND ITS SAFE LIMITS CONSUMPTION 马杜拉海峡西部黑鲳鱼和舌沙中铅、镉、铜、锌的污染及其安全食用限量

Journal of Biological Researches Pub Date : 2014-03-15 DOI: 10.23869/BPHJBR.18.2.20135

A. M. Charisma, B. Irawan, A. Soegianto

{"title":"CONTAMINATION OF Pb, Cd, Cu, Zn IN BLACK POMFRET (Formio niger) AND TONGUE SAND (Cynoglossus lingua) FISH CAUGHT FROM THE WESTERN PART OF MADURA STRAIT AND ITS SAFE LIMITS CONSUMPTION","authors":"A. M. Charisma, B. Irawan, A. Soegianto","doi":"10.23869/BPHJBR.18.2.20135","DOIUrl":"https://doi.org/10.23869/BPHJBR.18.2.20135","url":null,"abstract":"Contamination of Pb, Cd, Cu, Zn in the flesh on the two types of fish consisting pelagic fish include Black pomfert ( Formio niger ) whereas among other demersal fish include Tongue sand ( Cynoglossus lingua ), was conducted in March-December 2013, which was taken from the western part of Madura Strait (Gresik-Ujung pangkah, Surabaya-Kenjeran, and Sidoarjo-Sedati). Pb, Cd, Cu, and Zn detected using AAS (Atomic Absorption Spectrometry) brand Shimadzu AA-6200. The highest content of heavy metals, namely Pb in Cynoglossus lingua from the Sedati (5.63ppm±0.05), while a low of Cd in Formio niger from the Ujung pangkah (0.20ppm±0.004). The results show that there is a correlation between heavy metal with fish but not significantly. The content of Pb, Cd, Cu, Zn in 2 fish species originating from all locations stated below the exposure limits for consumption. Safe limit fish consumption of Formio niger is based on the WHO PTWI of 347.43 grams / week and Cynoglossus lingua 191.25 grams / week. Key words: Pelagic fish, Demersal fish, Black pomfert ( Formio niger ), Tongue sand( Cynoglossus lingua ), Pb, Cd, Cu, Zn, Western part of Madura Strait","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"74 1","pages":"102-105"},"PeriodicalIF":0.0,"publicationDate":"2014-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82203581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

COMPARATIVE STUDY OF EFFECTS DAIDZEIN CONTAINED IN YAM TUBER Pachyrhizus erosus AND PURE DAIDZEIN: THE DYNAMICS OF CHEMICAL COMPOUNDS AND ITS POTENTIAL IN MYOMETRIUM 红薯中大豆苷元与纯大豆苷元作用的比较研究:化合物动态及其在肌层中的潜力

Journal of Biological Researches Pub Date : 2014-03-15 DOI: 10.23869/BPHJBR.18.2.201310

C. Primiani, U. Lestari, Mohammad Amin, S. Sumitro

引用次数: 4

In Vitro Gentamicin Release from Bioactive BHAG(ELENA) Implant Against Staphylococcus aureus 生物活性BHAG(ELENA)植入物抗金黄色葡萄球菌庆大霉素体外释放研究

Journal of Biological Researches Pub Date : 2014-03-15 DOI: 10.23869/BPHJBR.18.2.20138

A. S. Budiatin, Muhamad Zainuddin, J. Khotib

{"title":"In Vitro Gentamicin Release from Bioactive BHAG(ELENA) Implant Against Staphylococcus aureus","authors":"A. S. Budiatin, Muhamad Zainuddin, J. Khotib","doi":"10.23869/BPHJBR.18.2.20138","DOIUrl":"https://doi.org/10.23869/BPHJBR.18.2.20138","url":null,"abstract":"Osteomyelitis is a bone-related infectious desease which is difficult to treat, because the antibiotic reaches the target is lower than the MIC and bacteria can’t be \u0000eradicated. This condition can cause the bacteria become resistant. To solve this problem, we can use local antibiotics as BHAG(ELENA) pellets implant, which \u0000can release gentamicin (GEN) continuously for more than a day with a concentration greater than MIC. BHAG(ELENA) pellet that have made contain BHA : \u0000GEL = 20 : 2 (dry state); GEN 10% and cross-linking with glutaraldehyde (GA) 0,5%, cylindrical weigh 100 mg; 4 mm in diameter and 3.2 mm thick. The release \u0000of GEN from BHAG(ELENA) pellet were tested in vitro, by soaking the pellet in phosphate buffer saline pH 7.4 at temperature 370C. The sample were sampled \u0000every day until 28 days. Then, the sample were tested by agar diffusion method that contain Staphylococcus aureus. Results showed that inhibition zone diameter \u0000greater than MIC GEN to S. aureus. Within 28 days, the release of GEN provide a total activity 99,24%, it showed that after 28 days, the pellets are still actively \u0000inhibit the bacterial growth. Furthermore, required to be tested in animal study (in vivo) with a defect in the femoral bone then filled with BHAG(ELENA) pellets \u0000as drug delivery system of GEN and bone fillers. \u0000 \u0000Keywords: agar diffusion method, Bovine Hydroxyapatite (BHA), gelatin (GEL), gentamicin (GEN), glutaraldehyde (GA) and drug delivery system (DDS).","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"10 1","pages":"116-118"},"PeriodicalIF":0.0,"publicationDate":"2014-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81997579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

DIVERSITY OF THE UNIONID FRESHWATER MUSSELS BIVALVIA: UNIONIDAE IN BRANTAS RIVER EAST JAVA INDONESIA 印度尼西亚爪哇东部布兰塔斯河淡水贝的多样性

Journal of Biological Researches Pub Date : 2014-03-15 DOI: 10.23869/BPHJBR.18.2.20137

M. Affandi, L. Chandra, Astra Budi Priatama, B. Irawan, A. Soegianto

{"title":"DIVERSITY OF THE UNIONID FRESHWATER MUSSELS BIVALVIA: UNIONIDAE IN BRANTAS RIVER EAST JAVA INDONESIA","authors":"M. Affandi, L. Chandra, Astra Budi Priatama, B. Irawan, A. Soegianto","doi":"10.23869/BPHJBR.18.2.20137","DOIUrl":"https://doi.org/10.23869/BPHJBR.18.2.20137","url":null,"abstract":"The aims of this study are to provide data and information about diversity, dominance, and distribution of species of the Unionidae mussels in Brantas River, East Java, Indonesia. Mussel samples were collected from 14 sampling stations along the Brantas River, starting from upstream in Tulungagung to downstream in Surabaya (± 170 km) in April-May 2012. The results showed that five species of mussels, namely: Contradens contradens, Elongaria orientalis, Rectidens sumatrensis, Pseudodon vondembuschianus and Pilsbryoconcha exilis were observed in the Brantas River. Contradens contradens was the ―dominant‖ species, followed by Elongaria orientalis as a \"common\" species, and Rectidens sumatrensis, Pseudodon vondembuschianus and Pilsbryoconcha exilis as \"rare\" species. Distribution of each species in the Brantas River tends to be concentrated in downstream of the river, mainly located in station 13. According to the IUCN Red List, Rectidens sumatrensis was categorized in ―Data Deficient‖, while Pseudodon vondembuschianus and Pilsbryoconcha exilis were categorized in ―Least Concern‖.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"27 1","pages":"111-115"},"PeriodicalIF":0.0,"publicationDate":"2014-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85006002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

PATCHOULI ALKOHOL ISOMERS POGOSTEMON HERBA PREDICTED VIRTUALLY 广藿香醇类异构体对广藿香的预测

Journal of Biological Researches Pub Date : 2014-02-15 DOI: 10.23869/BPHJBR.18.2.20134

S. J. Raharjo, C. Mahdi, N. Nurdiana, W. Nellen, F. Fatchiyah

{"title":"PATCHOULI ALKOHOL ISOMERS POGOSTEMON HERBA PREDICTED VIRTUALLY","authors":"S. J. Raharjo, C. Mahdi, N. Nurdiana, W. Nellen, F. Fatchiyah","doi":"10.23869/BPHJBR.18.2.20134","DOIUrl":"https://doi.org/10.23869/BPHJBR.18.2.20134","url":null,"abstract":"The aim of our research is predicting the alpha-patchouli alcohol isomer Pogostemon Herba as inhibitors cyclooxygenase (COX-1 and COX-2) isoenzymes. The data for the alpha-patchouli alcohol isomer (CD521903, CD442384, and/or CD6432585) Pogostemon Herba were explored from the pubchem database. Molecular interaction studies with COX-1 and COX-2 from mouse were done using the molecular docking tools Hex 6.12 and LeadIT2 Bisolve. The analysis of the alpha-patchouli alcohol compounds of patchouli oil showed that alpha-Patchouli alcohol (CD521903) binds to COX-1 at active sites including: LEU223B, ASP228B, LEU237B, ARG 332B, TRP 138A, GLU 139A, SER 142A, ASN 143A, and the interaction to COX-2 at active site including: GLN 289B, GLU 290B, ARG 222B, LYS 211B, THR 212B, HIS 214B, ASN 382B, HEM682B, GLN 454B, HIS 386B, TRP 387B, HIS 388B, VAL 274B, GLN 203B, VAL 291B, VAL 295B. The interaction hydrogen bond energy between alpha-patchouli alcohol: (CD521903-COX-1 complexes (-4 kJ/mol) and CD521903-COX-2 complexes (-8 kJ/ mol) by LeadIT2 Biosolve. This suggests alpha-patchouli alcohol CD521903 as candidate for a selective COX-2 inhibitor. These in silico data need further analyses of biological function activity. Keywords:alpha-patchouli alcohol, COX-1/ COX-2, inhibitor selective, predicted virtually","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"7 1","pages":"98-101"},"PeriodicalIF":0.0,"publicationDate":"2014-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78529245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

ISOLATION OF Pichia manshurica PROTOPLAST FROM Dahlia sp PLANT 大丽花毕赤酵母原生质体的分离

Journal of Biological Researches Pub Date : 2014-02-15 DOI: 10.23869/BPHJBR.18.2.20132

W. Wijanarka, E. Soetarto, K. Dewi, A. Indrianto

引用次数: 0