Effect of calcusol TM on CuZnSOD expression in mice renal of nephrolothiasis model

Abstract

This study aimed to determine the effect of traditional medicine, Calcusol TM , on number of cells expressing CuZnSOD in mice renal of nephrolithiasis model. Eight weeks old Swiss strain male mice ( Mus musculus ) were divided into five groups: (A) control, (B) nephrolithiasis, (C) Calcusol TM , (D) nephrolithiasis & Calcusol TM , and (E) nephrolithiasis & Calcusol TM simultaneously. Nephrolithiasis was induced by applying porang tuber ( Amor-phophallus muelleri ) flour 0.06 mg/g of body weight during 3 months. Calcusol TM is traditional medicine, made of tempuyung leaves extract with S accharum lactis as additional substance. The dosage for Calcusol TM treatment was 3.3 mg/g of body weight. After 3 months treatment, the mice were killed by neck-dislocation, the kidneys were isolated and prepared for paraffin histology. CuZnSOD was analyzed by immunohistochemistry (IHC), using rabbit policlonal antibody anti-SOD1 (Bioss, bs-1079R) as primary antibody. Tissues were observed under Olympus BX51, 400x magnification. Images were documented with Olympus Digital Camera DP20. The histology images were analized in Immunoratio software online (URL: http://153.1.200.58:8080/immunoratio/) to receive the percentage of number of cells expressing CuZnSOD. The result showed that Calcusol TM administration could decrease number of cells expressing CuZnSOD in kidney significantly (P<0.05). It was supposed that antioxidant content in Calcusol TM could scavenge ROS directly with no induction of CuZnSOD production in cell.