Journal of Biological Researches最新文献_第9页

Genetic diversity of Lactobacillus spp of natural Ethawah goat milk fermented was determined by using 16srDNA with DDGE analysis 采用16srDNA和DDGE分析法，对发酵的天然鄂西瓦羊奶乳酸菌进行了遗传多样性测定

Journal of Biological Researches Pub Date : 2014-02-15 DOI: 10.23869/BPHJBR.18.2.20133

Nur Kusmiyati, N. Hidayat, F. Fatchiyah

引用次数: 0

Human sperm protein 116 kDa: a candidate antigen for immunocontraception technology 人精子蛋白116 kDa:免疫避孕技术的候选抗原

Journal of Biological Researches Pub Date : 2014-02-01 DOI: 10.23869/BPHJBR.18.2.20131

U. Lestari, A. Aulanni’am, B. Purnomo, S. Sumitro

{"title":"Human sperm protein 116 kDa: a candidate antigen for immunocontraception technology","authors":"U. Lestari, A. Aulanni’am, B. Purnomo, S. Sumitro","doi":"10.23869/BPHJBR.18.2.20131","DOIUrl":"https://doi.org/10.23869/BPHJBR.18.2.20131","url":null,"abstract":"Spermatozoa membrane antigenic protein utilized as the substance candidate of immunocontraception has important criteria, that are only expressed in spermatozoa, cannot be found in the other tissue, and also should not have kinase activities. The observations done finally conclude 116kD, a sperm head’s protein is within the above criteria. It’s antigenic characters are shown with ability of it’s polyclonal antibody to bind the human spermatozoa, and interestingly this polyclonal antibody enable to block fertilization of other species in an in-vitro test experiments. This reseach also acquired that 116 kDa protein is specific and exists only in spermatozoa, and not in the other tissue such as spleen, kidney, pancreas, and epidydimic. This 116 kDa protein distribute along the whole area of the sperm, but mostly accumulated around the head up to the the neck. The imunohistological staining of the testis also indicate that this protein exists in the spermatid of the testes, but not be found in other somatic tissues, and it’s antibody is evidently was recognised by the goat and cow spermatozoa membrane protein resulted in blocking the fertilization of both species respectively. And the conclussion, 116 kD is Non-kinase protein specific only exists in the area of the head of human spermatozoa. It is therefore believed to be adequate candidate for antigen substance of immunocontraception technology.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"4 1","pages":"86-90"},"PeriodicalIF":0.0,"publicationDate":"2014-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84116993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Pemanfaatan Ekstrak Jintan Hitam Untuk Menurunkan Kadar Enzim Lp-Pla2 Sebagai Kandidat Pengobatan Aterosklerosis 黑孜然提取物的使用降低了中枢神经放松酶作为动脉粥样硬化治疗的候选人的水平

Journal of Biological Researches Pub Date : 2012-10-17 DOI: 10.23869/BPHJBR.18.1.20127

Retno Susilowati, E. Savitri, Kholifah Holil

{"title":"Pemanfaatan Ekstrak Jintan Hitam Untuk Menurunkan Kadar Enzim Lp-Pla2 Sebagai Kandidat Pengobatan Aterosklerosis","authors":"Retno Susilowati, E. Savitri, Kholifah Holil","doi":"10.23869/BPHJBR.18.1.20127","DOIUrl":"https://doi.org/10.23869/BPHJBR.18.1.20127","url":null,"abstract":"Ox-LDL deposits in the sub-endothelial easily occur in individuals who have hyperlipidemia accompanied with oxidative stress. The enzyme Lp-PLA2 is an enzyme marker of three proaterogenik conditions, those are hyperlipidemia, oxidative stress andinflammation. Black cumin seeds (Nigella sativa L.) have antioxidants ingredient that can inhibit lipid peroxidation, and expected toinhibit atherosclerosis through decreased levels of the enzyme Lp-PLA2 and F2-Isp. \u0000 \u0000This study used posttest only control group designusing experimental animals Rattusnorvegicus Wistar males. The study analysed the number of foam cells, lipid profi le, Lp-PLA2 andF2-Isp plasma levels in hyperlipidemia rats were given extracts of black cumin seeds with 3 different doses (0; 3.6 and 7.2 mg / kg). \u0000 \u0000The results showed that the extract of black cumin seeds can decrease serum levels of Lp-PLA2, tend to reduce the formation of foam cells,but not significant to decreasing levels of F2-Isp. Black cumin seeds extract can decrease cholesterol, TG, LDL and increase HDL inthe blood serum. The extract of black cumin seeds is anti atherogenic by reducing the enzyme Lp-PLA2 and improve lipid profiles.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"16 1","pages":"39-45"},"PeriodicalIF":0.0,"publicationDate":"2012-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81833307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KAJIAN HISTOLOGI OVARIUM IKAN SIDAT, Anguilla bicolor McClelland, YANG TERTANGKAP DI SEGARA ANAKAN CILACAP:

Journal of Biological Researches Pub Date : 2012-10-17 DOI: 10.23869/BPHJBR.18.1.20128

F. Rachmawati, U. Susilo

引用次数: 8

Eksplorasi dan identifikasi bakteri selulolitik pada limbah daduk tebu Saccharum officinarum L 用于勘探和鉴定甘蔗沙棘菌属污水的纤维素细菌

Journal of Biological Researches Pub Date : 2012-10-17 DOI: 10.23869/bphjbr.18.1.20125

E. Ekawati, Ni’matuzahroh Ni’matuzahroh, Tini Surtiningsih, Agus Supriyanto

引用次数: 3

Deteksi senyawa isoflavon daidzein dan genistein pada kultur invitro kalus kedelai Glycine max Merr 检测大豆乙酰菌菌培养中的二硝基苯丙胺和genistein化合物

Journal of Biological Researches Pub Date : 2012-10-17 DOI: 10.23869/bphjbr.18.1.201212

Tintrim Rahayu

引用次数: 1

Study on the habitat preference of Diadema setosum in Bama Coast Baluran National Park 巴马海岸Baluran国家公园中斑蝶栖息地偏好的研究

Journal of Biological Researches Pub Date : 2012-10-17 DOI: 10.23869/BPHJBR.18.1.20123

C. Puspita, Noer Moehammadi, B. Irawan

{"title":"Study on the habitat preference of Diadema setosum in Bama Coast Baluran National Park","authors":"C. Puspita, Noer Moehammadi, B. Irawan","doi":"10.23869/BPHJBR.18.1.20123","DOIUrl":"https://doi.org/10.23869/BPHJBR.18.1.20123","url":null,"abstract":"Diadema setosum is an ecologically important sea urchin which is exists on Bama Coast, but there is no report about the presence of D. setosum in Bama Coast yet. This study was done in order to know the habitat type occurs in Bama Coast, the presence status of D. setosum, distribution pattern, the habitat preference and the character of habitat which is preferred by D. setosum in Bama Coast. Quadrate transect method was used to collect the data. Eight line transect was positioned parallel to the shore line. The transect line was 160 m in length and placed 30 m distance between transects. Quadrate plots of 1 × 1 m2 placed 4 m distance from each plot along the transect. The habitat type was defi ned by estimated the dominant benthic cover of each plot. Measurement of physical and chemical factor of water includes sea water temperature and salinity. The Distribution pattern was determined by Poisson distribution and Chi-square simultaneously, then continued using Green coeffi cient, the habitat preference was analyzed by Chi-square and the character of habitat which is preferred by D. setosum was analyzed by selection index. The result of this study shows that there are fi ve habitat types in Bama Coast, which are sand, rubble, seagrass, live coral and dead coral. The distribution pattern of D. setosum in Bama Coast is clumped and only presence in three habitat types, which are seagrass, live coral and dead coral. Dadema setosum prefers several habitat type, which are dead coral and live coral and the character of habitat which is preferred by D. setosum is hard and solid substratum with simple architecturally structure.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"103 1","pages":"19-23"},"PeriodicalIF":0.0,"publicationDate":"2012-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76140507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Potensi dan konservasi jenis-jenis Hoya dataran tinggi Pulau Jawa 爪哇岛高地霍亚的潜力和保护

Journal of Biological Researches Pub Date : 2012-10-17 DOI: 10.23869/bphjbr.18.1.20121

S. Rahayu

引用次数: 2

Pemanfaatan biji labu kuning Cucurbita moschata untuk pemulihan morfologi dan motilitas spermatozoa mencit Mus musculus yang terpapar 2metoksietanol 使用黄瓜籽cucurita moschata用于形态学的恢复和精子精子的目的

Journal of Biological Researches Pub Date : 2012-10-17 DOI: 10.23869/bphjbr.18.1.20126

A. Hayati, N. Puspita, I. B. R. Pidada

{"title":"Pemanfaatan biji labu kuning Cucurbita moschata untuk pemulihan morfologi dan motilitas spermatozoa mencit Mus musculus yang terpapar 2metoksietanol","authors":"A. Hayati, N. Puspita, I. B. R. Pidada","doi":"10.23869/bphjbr.18.1.20126","DOIUrl":"https://doi.org/10.23869/bphjbr.18.1.20126","url":null,"abstract":"Research has done to investigate the effect of pumpkin seed extract on morphology and motility of the mices after given of 2-ME. Thirty male mices, 56–63 days with 23–27 g weight were used as experimental animals into 5 groups (2 groups is control and 3 groups is treatment), 6 mices each. The fi rst group is K0 which given 0.1 mL aquades by gavage for 40 days each day, and then K1 were given 0.05 mL 2-ME 200 mg/kg by intraperitoneally everyday within 5 days, and continued on the giving of aquadest. The next groups are treated groups which given 2-ME, in the fi rst 5 days, then continued to the givings of pumpkin seed extract by gavage. Cucurbita moschata seed extract were given in three doses: 0.5; 1.0; and 2.0 g/kg BB for 35 days. Sperm collected from cauda epididymis. Parameter of sperm quality are morphology and motility. The result shown that Cucurbita moschata seed extract doses 0.5–1.0 g/kg BB showed no signifi cant effect and then doses 2.0 g/kg BB increased recovery process morphology and motility with signifi cant differences p < 0.05. The conclutions were Cucurbita moschata seed extract increased recovery process morphology and motility of the mices.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"117 11 1","pages":"35-38"},"PeriodicalIF":0.0,"publicationDate":"2012-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84258420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect hydraulic agitation using anaerobic biofilter for treated domestic wastewater 厌氧生物滤池水力搅拌对生活污水处理的影响

Journal of Biological Researches Pub Date : 2012-10-17 DOI: 10.23869/bphjbr.18.1.201213

N. I. Oktavitri, A. Soegianto, T. W. C. Putranto, A. P. Dewayanti, A. L. H. Suryawan

引用次数: 0